FastQCFastQC Report
Thu 26 May 2016
SRR937833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191834
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30951.6133740629919617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19611.0222379765839216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.9914822190018454No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17940.9351835441058415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.7058185723073074No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.4253677658809179No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5620.2929616230699459No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5290.2757592501850558No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.24969504884431332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.23509909609349752No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4390.2288436877717193No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4190.2184180072354223No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4140.21581158710134804No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3530.18401326146564218No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3030.15794906012489965No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2590.13501256294504624No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2580.1344912789182314No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.13136357475734228No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.11885275811378587No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2260.11781019006015618No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.11103349771156312No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2050.10686322549704431No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1970.10269295328252552No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCAC405.3775456E-447.4988253
GGGGACG459.606167E-442.221187
CGCAGAA608.266131E-539.582359
TACTATA500.001612656637.999062
ATGCACT500.001612656637.999064
ATACTAT801.0731195E-535.624126
CCTAATC550.002574604534.54463
TATACTG550.002574604534.54465
GCACTGC550.002574604534.54466
GGAGGGT550.002574604534.54468
TAATCAT1201.4950638E-731.6658825
ATCATCA600.00394329931.6658827
AATCATC804.480495E-429.6867666
ACTTATT650.005832803429.2300478
GGGGCCG650.005832803429.2300479
CTTAATC700.00837546327.1421893
TCATCAG700.00837546327.1421898
TCAATAC1000.001648881823.7494133
TACAGTG1503.016825E-522.1661195
GAGTACT17150.022.15688712-13
Produced by FastQC (version 0.11.5)