##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191834 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.172300009383115 33.0 31.0 34.0 31.0 34.0 2 32.368469614354076 34.0 31.0 34.0 31.0 34.0 3 32.409442538861725 34.0 31.0 34.0 30.0 34.0 4 36.03015628095124 37.0 35.0 37.0 35.0 37.0 5 35.96452662197525 37.0 35.0 37.0 35.0 37.0 6 36.03505113796303 37.0 35.0 37.0 35.0 37.0 7 35.99259776681923 37.0 35.0 37.0 35.0 37.0 8 35.97557263050346 37.0 35.0 37.0 35.0 37.0 9 37.620286289187526 39.0 37.0 39.0 35.0 39.0 10-11 37.56303366452245 39.0 37.0 39.0 35.0 39.0 12-13 37.48081935423335 39.0 37.0 39.0 35.0 39.0 14-15 38.6070587070071 40.0 38.0 41.0 34.0 41.0 16-17 38.59035676678795 40.0 38.0 41.0 34.5 41.0 18-19 38.59942971527467 40.0 38.0 41.0 34.0 41.0 20-21 38.527320495845366 40.0 38.0 41.0 34.0 41.0 22-23 38.45550058905095 40.0 38.0 41.0 34.0 41.0 24-25 38.39203686520638 40.0 38.0 41.0 34.0 41.0 26-27 38.140697165257464 40.0 37.0 41.0 34.0 41.0 28-29 38.02963499692443 40.0 37.0 41.0 33.5 41.0 30-31 37.917016795771346 40.0 37.0 41.0 33.5 41.0 32-33 37.63755642899591 40.0 35.5 41.0 33.0 41.0 34-35 37.377878791038086 40.0 35.0 41.0 33.0 41.0 36-37 37.11650697999312 39.5 35.0 41.0 33.0 41.0 38-39 36.8970333726034 39.0 35.0 41.0 32.5 41.0 40-41 36.62120896191499 39.0 35.0 41.0 31.0 41.0 42-43 36.25972715994037 39.0 35.0 41.0 30.5 41.0 44-45 35.79340471449274 39.0 35.0 41.0 29.5 41.0 46-47 35.23292012886141 39.0 35.0 41.0 26.0 41.0 48-49 34.708995798450744 39.0 35.0 40.5 23.5 41.0 50-51 34.334247317993686 38.5 34.5 40.0 21.0 41.0 52-53 34.04054286518553 38.0 34.0 40.0 19.0 41.0 54-55 33.76443696112264 38.0 34.0 40.0 14.5 41.0 56-57 33.41536693182647 38.0 33.5 40.0 8.5 41.0 58-59 33.15038262247568 38.0 33.0 40.0 4.5 41.0 60-61 32.75736313687876 37.5 33.0 40.0 2.0 41.0 62-63 32.3291387345309 37.0 32.5 40.0 2.0 41.0 64-65 31.918669266136348 36.0 32.0 39.5 2.0 41.0 66-67 31.456863746781075 36.0 31.0 39.0 2.0 40.5 68-69 30.975666461628286 35.0 31.0 39.0 2.0 40.0 70-71 30.520392631129 35.0 30.5 38.0 2.0 40.0 72-73 30.037227498774982 35.0 29.5 37.5 2.0 40.0 74-75 29.547038064159636 35.0 29.0 37.0 2.0 39.0 76-77 28.281146720602187 33.5 27.5 35.5 2.0 38.5 78-79 28.78999551695737 34.0 29.0 36.0 2.0 38.5 80-81 28.577921536328283 34.0 29.0 36.0 2.0 37.5 82-83 28.153679743945286 34.0 28.5 35.0 2.0 37.0 84-85 27.733868865790214 34.0 27.0 35.0 2.0 37.0 86-87 27.41817925915114 34.0 26.5 35.0 2.0 36.0 88-89 27.00184795187506 34.0 26.0 35.0 2.0 36.0 90-91 26.548622767601152 33.0 25.0 35.0 2.0 35.0 92-93 26.113048260475203 33.0 24.0 35.0 2.0 35.0 94-95 25.63453558806051 33.0 21.5 35.0 2.0 35.0 96-97 25.173178372968295 33.0 19.0 35.0 2.0 35.0 98-99 24.492657714482313 32.0 4.5 34.0 2.0 35.0 100-101 22.389534701877665 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31613961845812355 1101 2 0.24123314917247285 1101 3 0.340454257672242 1101 4 0.25911896055358596 1101 5 0.16126416462839188 1101 6 0.15096090673385731 1101 7 0.2934215866914087 1101 8 0.2592986380018232 1101 9 0.42023389949789447 1101 10-11 0.35919120144537686 1101 12-13 0.4752699376100509 1101 14-15 0.5807932905168869 1101 16-17 0.5605942432988797 1101 18-19 0.7252424030794415 1101 20-21 0.5691009586285034 1101 22-23 0.27388252410322167 1101 24-25 0.25682532880797737 1101 26-27 0.3800265284247146 1101 28-29 0.6508803241811734 1101 30-31 0.25156593369228375 1101 32-33 0.41859677828430364 1101 34-35 0.45003235447897794 1101 36-37 0.5685886307617238 1101 38-39 0.6605696349021102 1101 40-41 0.21235202883082138 1101 42-43 0.6855841045255104 1101 44-45 1.0809593153767736 1101 46-47 1.032611369784334 1101 48-49 1.0089734193308288 1101 50-51 1.3135463965064744 1101 52-53 1.184387514641763 1101 54-55 1.5551904002207806 1101 56-57 1.6058343989630686 1101 58-59 1.5781920775535667 1101 60-61 1.7843842515573485 1101 62-63 1.6728288431969531 1101 64-65 1.9411515139318851 1101 66-67 1.983234902704634 1101 68-69 1.555048288253051 1101 70-71 1.448531770128831 1101 72-73 1.3813094711944736 1101 74-75 1.3914814166436749 1101 76-77 1.3041604544608525 1101 78-79 1.4084174516895303 1101 80-81 1.6261371328097916 1101 82-83 1.059295079111262 1101 84-85 1.1134046261543915 1101 86-87 1.4211858783970506 1101 88-89 1.7773450782463271 1101 90-91 1.5794585654170312 1101 92-93 1.6369381159216694 1101 94-95 2.2169337548755017 1101 96-97 2.29537062781586 1101 98-99 2.595945525291693 1101 100-101 2.615436425884482 1102 1 0.3180782595272049 1102 2 0.3088032602208486 1102 3 0.0043353041619837995 1102 4 0.06883098765691642 1102 5 0.06595078235849172 1102 6 0.31678059429267336 1102 7 0.22063784869027359 1102 8 0.16454990847048379 1102 9 0.2028049516341639 1102 10-11 0.49130112051181385 1102 12-13 0.3552812306648221 1102 14-15 0.592058112651273 1102 16-17 0.3848460784202814 1102 18-19 0.4133752846905878 1102 20-21 0.43664283703994045 1102 22-23 0.28969280078305815 1102 24-25 0.6830910920302671 1102 26-27 0.46225878891784333 1102 28-29 0.6708878528843556 1102 30-31 0.6302126492955225 1102 32-33 0.6660840735976947 1102 34-35 0.785624298766578 1102 36-37 0.9165464700239099 1102 38-39 0.5470556126924322 1102 40-41 -0.2480469924377715 1102 42-43 -0.4134089314240441 1102 44-45 0.04680723557252264 1102 46-47 -0.11511119750345244 1102 48-49 -0.24107363506405477 1102 50-51 -0.20625409285923268 1102 52-53 0.25175058635265657 1102 54-55 -0.35964114504554345 1102 56-57 -0.32991753026712445 1102 58-59 -0.3697281181927181 1102 60-61 -0.4774358124366316 1102 62-63 -0.5672238577253168 1102 64-65 -0.26936984876339665 1102 66-67 -0.009537542241723429 1102 68-69 0.27355948719903367 1102 70-71 0.4644832099933147 1102 72-73 0.6014111086874188 1102 74-75 0.376800445440967 1102 76-77 0.0904205711557502 1102 78-79 -0.002583865833525323 1102 80-81 0.21948364137369225 1102 82-83 -0.05598818834591768 1102 84-85 -0.3490177340940548 1102 86-87 -0.10657621458242872 1102 88-89 -0.029054319457415545 1102 90-91 0.13566974554125366 1102 92-93 0.4632227009019054 1102 94-95 0.5806077620277712 1102 96-97 0.5921709289639878 1102 98-99 0.8910801008610534 1102 100-101 1.3734921382880216 1103 1 0.2906061548715684 1103 2 0.28860318326817236 1103 3 0.3646277243235829 1103 4 0.45455626661036064 1103 5 0.16298810440542866 1103 6 0.11862745847120948 1103 7 -0.028265575703720458 1103 8 -0.041413923772687156 1103 9 0.3271219966514778 1103 10-11 0.11329034713744335 1103 12-13 0.2607256323577829 1103 14-15 0.29961871288212905 1103 16-17 0.3322296875006927 1103 18-19 0.23122830508305015 1103 20-21 0.2225220213415966 1103 22-23 0.06893481694311987 1103 24-25 0.4126024425496979 1103 26-27 0.3493499778366598 1103 28-29 0.2726000498832022 1103 30-31 0.41945851308928894 1103 32-33 0.7774923075338194 1103 34-35 0.670964814349496 1103 36-37 0.5626218836445318 1103 38-39 0.8506531502068597 1103 40-41 1.1262508144110228 1103 42-43 1.088938125136174 1103 44-45 1.0952297057533613 1103 46-47 0.8472974211960462 1103 48-49 1.0839552221887345 1103 50-51 1.5402253223004365 1103 52-53 1.5404385432591567 1103 54-55 1.6036139530691145 1103 56-57 1.715946263499717 1103 58-59 1.7436424089331055 1103 60-61 1.9521140144198554 1103 62-63 1.756631471247367 1103 64-65 1.6707994471196344 1103 66-67 1.6704547624908663 1103 68-69 1.669153946606496 1103 70-71 1.9987271499702288 1103 72-73 2.0477558566674112 1103 74-75 2.022529572028116 1103 76-77 2.0107937916251615 1103 78-79 2.0050921634084915 1103 80-81 2.0568826409889276 1103 82-83 2.381968718156582 1103 84-85 2.1563881912618506 1103 86-87 2.0830159362448093 1103 88-89 2.304516284621073 1103 90-91 2.1132765173765726 1103 92-93 2.5627225085202205 1103 94-95 2.589611224898107 1103 96-97 2.19501817790589 1103 98-99 2.3521612859322367 1103 100-101 1.72279956422107 1104 1 -0.008252500326513257 1104 2 0.23281788842076878 1104 3 0.23659861176052033 1104 4 0.09544618473127997 1104 5 0.19549161941660032 1104 6 0.1806570672711416 1104 7 0.28601777554927565 1104 8 0.36926343955541086 1104 9 0.10646200161384911 1104 10-11 0.32204065728548414 1104 12-13 0.23622393688180665 1104 14-15 0.20483747063583024 1104 16-17 0.16257870743287128 1104 18-19 0.23038056709610544 1104 20-21 0.013440886613288683 1104 22-23 0.027705803627434022 1104 24-25 0.017101656841319368 1104 26-27 -0.026384039200813447 1104 28-29 0.04447582525331484 1104 30-31 -0.06381416907099435 1104 32-33 -0.0992755363169735 1104 34-35 -0.5725634297990467 1104 36-37 -0.4804059883263534 1104 38-39 -0.44615852790082045 1104 40-41 -0.38419083640363283 1104 42-43 -0.49695220651546634 1104 44-45 -0.8888286847850537 1104 46-47 -1.2038149764550994 1104 48-49 -1.0502975277905904 1104 50-51 -0.9711260961099484 1104 52-53 -0.986689072322676 1104 54-55 -0.6807504502060482 1104 56-57 -1.2973901023922707 1104 58-59 -1.393153555007018 1104 60-61 -1.3381225251550362 1104 62-63 -1.668788262438845 1104 64-65 -0.9590285240986276 1104 66-67 -1.094990610100318 1104 68-69 -1.4244900861451377 1104 70-71 -1.7623554734686948 1104 72-73 -1.2607587409671943 1104 74-75 -1.0356335244899562 1104 76-77 -1.1524888152725303 1104 78-79 -0.8019337236149156 1104 80-81 -0.9954289957656819 1104 82-83 -0.9302263029334874 1104 84-85 -0.704686161562563 1104 86-87 -0.5907289980038044 1104 88-89 -0.5194850386772458 1104 90-91 -0.25331847063103297 1104 92-93 -0.5533762426169879 1104 94-95 -0.7715458340713752 1104 96-97 -0.6205677951319082 1104 98-99 -0.45790405192236605 1104 100-101 -0.3985923802003981 1105 1 0.2882415905600979 1105 2 0.1180970260363523 1105 3 0.018183935401921758 1105 4 -0.11221341077878577 1105 5 0.14154314490737363 1105 6 0.31931107508402334 1105 7 0.3299776232474443 1105 8 0.4271678058709867 1105 9 0.3548178340818282 1105 10-11 0.2887246309788054 1105 12-13 0.2816672440073589 1105 14-15 0.5110482207718121 1105 16-17 0.3821430648476962 1105 18-19 0.42822460606164725 1105 20-21 0.4683313578589008 1105 22-23 0.28831253853323346 1105 24-25 0.641625713608363 1105 26-27 0.821103969502154 1105 28-29 1.0517942250950796 1105 30-31 0.6416573237836687 1105 32-33 0.6764130361005627 1105 34-35 1.0707634752101 1105 36-37 1.2763808385539335 1105 38-39 1.5333450076774824 1105 40-41 1.7125925290713155 1105 42-43 1.4037178226592317 1105 44-45 2.054985289402751 1105 46-47 1.7098600470330112 1105 48-49 2.0621239724813734 1105 50-51 2.0898811728412454 1105 52-53 1.7305635608178065 1105 54-55 1.818297663328046 1105 56-57 1.5483548414835155 1105 58-59 1.8670328663943536 1105 60-61 2.1961206632937618 1105 62-63 2.133627303995418 1105 64-65 2.075350648131014 1105 66-67 2.114548034017769 1105 68-69 2.3503825835873435 1105 70-71 2.4754677970648586 1105 72-73 2.501319091204092 1105 74-75 2.2522317773940372 1105 76-77 2.0357621860625805 1105 78-79 2.176815720087802 1105 80-81 2.1908316975043576 1105 82-83 2.2993143191305023 1105 84-85 2.0128755256252937 1105 86-87 2.354086311297486 1105 88-89 2.5931218940231417 1105 90-91 2.0979770839355467 1105 92-93 2.4327534117369645 1105 94-95 2.6248221627639126 1105 96-97 2.8593436917889257 1105 98-99 3.376368805714975 1105 100-101 3.3419876524357086 1106 1 -0.44072343718541873 1106 2 -0.4035509285384329 1106 3 -0.6692596057001587 1106 4 -0.23074483418825764 1106 5 0.016268915974606557 1106 6 -0.04854284155037902 1106 7 -0.1187576974391007 1106 8 -0.10703015515623804 1106 9 -0.4064739455026327 1106 10-11 -0.40413896432381335 1106 12-13 -0.2352542942556468 1106 14-15 -0.4247499467336695 1106 16-17 -0.6087912492573508 1106 18-19 -0.6459301235808894 1106 20-21 -0.6606530272230486 1106 22-23 -0.6765055025043196 1106 24-25 -0.7995441147565856 1106 26-27 -0.5486372874342251 1106 28-29 -0.551461033647989 1106 30-31 -0.7091298745645034 1106 32-33 -0.44860309501312656 1106 34-35 -0.5379440999608818 1106 36-37 -0.1964704971022826 1106 38-39 0.09196547483453799 1106 40-41 0.08888301816668331 1106 42-43 -0.11309079780792075 1106 44-45 -0.018330622327795254 1106 46-47 0.07892379719432085 1106 48-49 0.06857641795821223 1106 50-51 0.23656138964341267 1106 52-53 0.10246638698892241 1106 54-55 0.021323860256678984 1106 56-57 -0.2047584634590578 1106 58-59 -0.11662631543556046 1106 60-61 -0.0030985908035461307 1106 62-63 -0.08761092029158846 1106 64-65 0.0927722509184683 1106 66-67 -0.017517393779368007 1106 68-69 -0.1110640546885584 1106 70-71 0.5510420647122984 1106 72-73 -0.05914032291385496 1106 74-75 -0.006285450635385104 1106 76-77 0.15335800487791218 1106 78-79 0.40518389662670984 1106 80-81 0.04973284603965311 1106 82-83 0.3061539113359508 1106 84-85 0.1595718735411502 1106 86-87 0.5686623856296578 1106 88-89 0.08457240376633735 1106 90-91 -0.5015518875446432 1106 92-93 -0.3048578929475134 1106 94-95 -0.6262586218534594 1106 96-97 -0.788820587472916 1106 98-99 -0.4039092946850751 1106 100-101 -0.21603596351473442 1107 1 -0.06012757426476156 1107 2 0.05453798779377195 1107 3 -0.17497124509503692 1107 4 -0.17084980485821433 1107 5 -0.10020221709115162 1107 6 0.03142010997787281 1107 7 -0.029609812289358217 1107 8 -0.03710116472709757 1107 9 0.15674000942172484 1107 10-11 -0.11786233573474902 1107 12-13 0.08216620589872292 1107 14-15 0.027858883152099168 1107 16-17 0.11558355778186069 1107 18-19 -0.21620644343269646 1107 20-21 -0.23768297473771582 1107 22-23 -0.0017216185895350122 1107 24-25 -0.15771793383655108 1107 26-27 -0.2295959166407684 1107 28-29 -0.4856614041327063 1107 30-31 -0.08717865505230549 1107 32-33 0.26234164101651203 1107 34-35 -0.14434267976946558 1107 36-37 0.30727294223084556 1107 38-39 0.26563018616518974 1107 40-41 0.20222818642849205 1107 42-43 0.017273512164287297 1107 44-45 0.08783640453234653 1107 46-47 -0.37094498946823506 1107 48-49 0.6337199807862461 1107 50-51 0.8550007227709244 1107 52-53 0.5484759578441327 1107 54-55 0.7259394823622642 1107 56-57 0.3351072358308471 1107 58-59 0.5143940612239035 1107 60-61 0.32894833726066963 1107 62-63 0.5463424603814531 1107 64-65 1.1300522756174445 1107 66-67 1.349378253025204 1107 68-69 1.4746877205507083 1107 70-71 0.9632449050951308 1107 72-73 1.5933758857925362 1107 74-75 1.581395932507558 1107 76-77 1.0719285513428716 1107 78-79 1.388087570600156 1107 80-81 1.2545414289356067 1107 82-83 0.928876974009615 1107 84-85 1.280674065575731 1107 86-87 1.8412773902607178 1107 88-89 1.7460419931457736 1107 90-91 1.3692415672253482 1107 92-93 1.8130025578088969 1107 94-95 1.3567222364361378 1107 96-97 1.3983899713415333 1107 98-99 1.3384108658583003 1107 100-101 1.901863079399746 1108 1 0.04630851059884833 1108 2 -0.06749802575588149 1108 3 -0.11274981525014027 1108 4 -0.17183037180419802 1108 5 0.012919551495052417 1108 6 0.1603200921493837 1108 7 0.07593484807790674 1108 8 -0.04004286556503445 1108 9 -0.29646020452015165 1108 10-11 0.026726717572437053 1108 12-13 0.21703873219575343 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2316 62-63 -0.5028704575023468 2316 64-65 -0.9530376302572634 2316 66-67 -0.3874360929663645 2316 68-69 -0.4973992641945024 2316 70-71 -0.32606862947156756 2316 72-73 -0.0914261265411227 2316 74-75 -0.49792084775858925 2316 76-77 0.16625669155708778 2316 78-79 0.27205381532589357 2316 80-81 -0.25061111893845833 2316 82-83 -0.30696366214747783 2316 84-85 -0.09355508080531294 2316 86-87 -0.2576353004241234 2316 88-89 -0.0072037063024552594 2316 90-91 -0.15680517084670598 2316 92-93 -0.6264651474815963 2316 94-95 -0.44411353117178365 2316 96-97 -0.5584422259969948 2316 98-99 -0.817058137711971 2316 100-101 0.12159693204498723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 11.0 6 26.0 7 57.0 8 104.0 9 189.0 10 339.0 11 505.0 12 787.0 13 1181.0 14 1415.0 15 2073.0 16 2809.0 17 3825.0 18 3292.0 19 2128.0 20 1790.0 21 1826.0 22 1792.0 23 1863.0 24 1952.0 25 2113.0 26 2237.0 27 2708.0 28 3092.0 29 3652.0 30 4386.0 31 5365.0 32 6641.0 33 8725.0 34 11610.0 35 16727.0 36 22929.0 37 36347.0 38 34052.0 39 3280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.309778357235984 23.29856584093872 15.50664928292047 18.885006518904824 2 15.344516613321934 25.968285079808584 37.869199411991616 20.817998894877864 3 20.16743642941293 28.57522649790965 31.540811326459334 19.716525746218082 4 12.46598621725033 21.655181041942512 37.1769342243815 28.70189851642566 5 11.504134127141352 42.19520587223305 33.503112325224954 12.797547675400638 6 27.154206240812368 40.78838996215478 16.82913352169063 15.228270275342224 7 24.228238998300615 38.803861672070646 20.65848598267252 16.309413346956223 8 24.820417652762284 36.42993421395582 19.900539007683726 18.849109125598172 9 24.94344068309059 19.65292909494667 22.02112242876654 33.382507793196204 10-11 23.28940646600707 30.357496585589622 26.623017817488037 19.73007913091527 12-13 25.790719376078492 29.193666946444303 26.95431630860342 18.06129736887378 14-15 21.58949925456384 31.715962759469125 27.80085907607619 18.893678909890845 16-17 18.056496762826193 34.049751347519205 28.871576467153893 19.022175422500702 18-19 17.75936486754173 32.491894033383026 32.61126807552363 17.13747302355161 20-21 19.965751550172413 30.406315893730763 32.366870228609706 17.261062327487117 22-23 19.016055882812907 31.810978470520773 31.852421414794353 17.32054423187197 24-25 19.204364189872493 31.880949153956024 32.2805133605096 16.634173295661874 26-27 18.706097309864695 31.626357643857467 33.05678711153857 16.61075793473926 28-29 17.79912162954661 33.062697926576575 32.32794234553582 16.810238098341003 30-31 18.504540383873557 31.96253010415255 32.40145125473065 17.13147825724324 32-33 18.010884410479893 32.63238007860963 32.433510222379766 16.92322528853071 34-35 18.774304867750242 32.56591636519074 32.204145250581234 16.45563351647779 36-37 18.38729940286759 32.29806055772323 32.211787826422395 17.102852212986782 38-39 18.1513704557065 32.92403849161254 31.890853550465508 17.033737502215455 40-41 18.59138263129224 32.810484091673246 31.36522036036459 17.232912916669925 42-43 17.866416392929768 33.474112310339436 30.978413890192396 17.6810574065384 44-45 18.66926613634705 32.91986821939802 30.53603636477371 17.87482927948122 46-47 19.37769112878843 33.03585391536433 30.036906909098494 17.54954804674875 48-49 19.238768935642277 33.27799034581982 29.54507542979868 17.93816528873922 50-51 18.49698176548474 33.78181135773637 29.770009487369286 17.951197389409593 52-53 19.20201841175183 34.547316951113984 28.343776390003857 17.906888247130333 54-55 18.73182021956483 33.66608630378348 28.738127756289295 18.863965720362398 56-57 19.501756727170367 33.631681558013696 28.10789536787014 18.758666346945795 58-59 19.84554354285476 33.753140736261564 27.947079245597756 18.454236475285924 60-61 18.92443466747292 33.73515643733645 29.180176611028287 18.160232284162348 62-63 18.764661113254167 33.44010967815924 29.149942137473023 18.645287071113568 64-65 18.849109125598172 34.29970703837693 28.474879322747793 18.376304513277102 66-67 18.933557137942177 33.54697290365628 27.95463786398657 19.564832094414964 68-69 18.797502006943503 33.18702628314063 28.677137525151952 19.338334184763912 70-71 19.693328607024824 33.2104840643473 28.080267314448953 19.015920014178924 72-73 18.998978283307444 32.6347258567303 29.096771166737913 19.269524693224348 74-75 18.43442768226696 34.259828810325594 28.17983256357059 19.12591094383686 76-77 18.702107030036387 32.619608619952665 28.693297329983213 19.984987020027734 78-79 18.63694652668453 32.920650145438245 28.34742537819156 20.094977949685667 80-81 18.83425253083395 32.79032913873453 28.245253708935852 20.13016462149567 82-83 18.47300270025126 32.92482041765276 28.565582743413575 20.036594138682403 84-85 18.13499727628386 33.11595732757834 28.258098820070533 20.490946576067266 86-87 17.98403828309893 33.020736678586694 27.90459459741235 21.090630440902032 88-89 18.345288113681622 32.48798440318192 27.764108552185746 21.402618930950716 90-91 18.473441234968888 31.832198967209823 28.20283561118917 21.49152418663212 92-93 18.220844823721375 32.47248823158367 28.378695386989317 20.92797155770564 94-95 17.900372196795146 32.72021643712794 28.13708727337177 21.24232409270515 96-97 17.246421385155916 32.56904406935163 28.622142060322986 21.562392485169468 98-99 18.003372716444208 33.10474969179001 27.398499219375132 21.49337837239064 100-101 17.43624367520053 33.166323000763796 27.65602475437229 21.74140856966338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2060.0 1 1280.5 2 447.5 3 406.5 4 391.0 5 427.0 6 636.0 7 912.0 8 1057.0 9 1053.5 10 931.0 11 815.5 12 795.5 13 799.0 14 772.5 15 720.0 16 685.0 17 686.5 18 727.0 19 805.5 20 911.0 21 994.0 22 1078.5 23 1299.5 24 1562.0 25 1802.5 26 2174.0 27 2672.5 28 3046.5 29 3475.5 30 4015.5 31 4593.0 32 5140.5 33 5602.0 34 6168.5 35 6666.0 36 7023.0 37 7262.0 38 7026.5 39 6557.5 40 6420.5 41 6204.5 42 5903.0 43 5775.5 44 5633.5 45 5487.0 46 5620.0 47 6566.5 48 8561.5 49 7952.0 50 5469.0 51 4293.5 52 3550.5 53 3104.5 54 2677.5 55 2324.0 56 2018.5 57 1752.0 58 1567.5 59 1398.5 60 1197.5 61 1052.0 62 864.5 63 579.5 64 366.0 65 252.5 66 184.0 67 161.0 68 123.0 69 84.0 70 66.0 71 38.0 72 29.0 73 25.0 74 16.5 75 12.5 76 14.5 77 10.0 78 9.0 79 10.0 80 3.5 81 2.5 82 3.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04378785825244743 2 0.0 3 0.0 4 0.0 5 0.008340544429037606 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007819260402222755 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.606420134074252E-4 22-23 0.0020851361072594015 24-25 0.0 26-27 0.0018244940938519762 28-29 7.819260402222756E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.606420134074252E-4 38-39 0.0 40-41 2.606420134074252E-4 42-43 0.022936497179853417 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.606420134074252E-4 86-87 0.0 88-89 0.0 90-91 0.012250174630148982 92-93 0.0036489881877039524 94-95 0.0 96-97 0.0 98-99 2.606420134074252E-4 100-101 0.015377878791038085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 191834.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.295464141768235 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7420005619591 44.737105419272446 2 14.51535640524178 16.923588776535162 3 4.332444046445944 7.576855096674204 4 1.8157378766308023 4.2339712911192535 5 0.814283861987752 2.373452783886378 6 0.4880533139547531 1.7070776677752277 7 0.2723424356913264 1.1113430085887877 8 0.1709578466103832 0.7972853613463606 9 0.13318783700242062 0.6987822098484856 >10 0.6231995231629095 6.590915413544512 >50 0.05267874666214004 2.200062029231673 >100 0.031806035720537376 4.08897491184828 >500 0.0029818158488003794 1.104956094217751 >1k 0.004969693081333966 5.855629936111479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3095 1.6133740629919617 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 1.0222379765839216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.9914822190018454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1794 0.9351835441058415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1354 0.7058185723073074 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.4253677658809179 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 562 0.2929616230699459 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 529 0.2757592501850558 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.24969504884431332 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 451 0.23509909609349752 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 439 0.2288436877717193 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 419 0.2184180072354223 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 414 0.21581158710134804 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 353 0.18401326146564218 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 303 0.15794906012489965 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 259 0.13501256294504624 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 258 0.1344912789182314 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.13136357475734228 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.11885275811378587 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 226 0.11781019006015618 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.11103349771156312 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 205 0.10686322549704431 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 197 0.10269295328252552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.606420134074252E-4 0.0 20-21 0.0 0.0 0.0 5.212840268148504E-4 0.0 22-23 0.0 0.0 0.0 0.0018244940938519764 0.0 24-25 0.0 0.0 0.0 0.003127704160889102 0.0 26-27 0.0 0.0 0.0 0.005473482281555929 0.0 28-29 0.0 0.0 0.0 0.01772365691170491 0.0 30-31 0.0 0.0 0.0 0.033101535702743 0.0 32-33 0.0 0.0 0.0 0.05212840268148503 0.0 34-35 0.0 0.0 0.0 0.06750628147252312 0.0 36-37 0.0 0.0 0.0 0.08340544429037605 0.0 38-39 0.0 0.0 0.0 0.10894836160430373 0.0 40-41 0.0 0.0 0.0 0.12615073448919378 0.0 42-43 0.0 0.0 0.0 0.16889602468801151 0.0 44-45 0.0 0.0 0.0 0.23275331797283066 0.0 46-47 0.0 0.0 0.0 0.28931263488224196 0.0 48-49 0.0 0.0 0.0 0.351866718100024 0.0 50-51 0.0 0.0 0.0 0.4300593221222515 0.0 52-53 0.0 0.0 0.0 0.5562100566114453 0.0 54-55 0.0 0.0 0.0 0.6484773293576738 0.0 56-57 0.0 0.0 0.0 0.8032986853216844 0.0 58-59 0.0 0.0 0.0 1.0128548641012542 0.0 60-61 0.0 0.0 0.0 1.1872243710708217 0.0 62-63 0.0 0.0 0.0 1.4218021831375043 0.0 64-65 0.0 0.0 0.0 1.6065973706433687 0.0 66-67 0.0 0.0 0.0 1.9303147512953909 0.0 68-69 0.0 0.0 0.0 2.3043360405350457 0.0 70-71 0.0 0.0 0.0 2.6202341607848454 0.0 72-73 0.0 0.0 0.0 3.0586340273361343 0.0 74-75 0.0 0.0 0.0 3.4941668317399417 0.0 76-77 0.0 0.0 0.0 3.8210119165528527 0.0 78-79 0.0 0.0 0.0 4.195293847805916 0.0 80-81 0.0 0.0 0.0 4.782259661999436 0.0 82-83 0.0 0.0 0.0 5.28816581002325 0.0 84-85 0.0 0.0 0.0 5.941334695622256 0.0 86-87 0.0 0.0 0.0 6.743069528863497 0.0 88-89 0.0 0.0 0.0 7.792674916855198 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGCAC 40 5.3775456E-4 47.498825 3 GGGGACG 45 9.606167E-4 42.22118 7 CGCAGAA 60 8.266131E-5 39.58235 9 TACTATA 50 0.0016126566 37.99906 2 ATGCACT 50 0.0016126566 37.99906 4 ATACTAT 80 1.0731195E-5 35.62412 6 CCTAATC 55 0.0025746045 34.5446 3 TATACTG 55 0.0025746045 34.5446 5 GCACTGC 55 0.0025746045 34.5446 6 GGAGGGT 55 0.0025746045 34.5446 8 TAATCAT 120 1.4950638E-7 31.665882 5 ATCATCA 60 0.003943299 31.665882 7 AATCATC 80 4.480495E-4 29.686766 6 ACTTATT 65 0.0058328034 29.230047 8 GGGGCCG 65 0.0058328034 29.230047 9 CTTAATC 70 0.008375463 27.142189 3 TCATCAG 70 0.008375463 27.142189 8 TCAATAC 100 0.0016488818 23.749413 3 TACAGTG 150 3.016825E-5 22.166119 5 GAGTACT 1715 0.0 22.156887 12-13 >>END_MODULE