FastQCFastQC Report
Thu 26 May 2016
SRR937831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427669
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150400.43878215778711427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107540.3137409125560257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.21463566056115685No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45730.13341428241758466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167800.034.5774651
GGTATCA110750.033.709411
ATCAACG203700.028.2413943
TCAACGC205000.028.0871374
TATCAAC206250.028.0078952
CAACGCA206950.027.7999175
AACGCAG214300.026.8652616
GTACATG142000.026.5589031
TACATGG143100.025.6493172
ACGCAGA240100.023.8010887
CGCAGAG240600.023.6719598
ACATGGG147000.023.6100713
GGACCGA7950.022.7052946
GAGTACT128250.022.48154312-13
GCAGAGT255450.021.719079
AGTACTT135350.020.86355812-13
GTACTTT139600.020.61862814-15
AGAGTAC208000.020.39353210-11
CATGGGG71400.020.2438264
CATGGGA73800.019.263364
Produced by FastQC (version 0.11.5)