##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937831_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3427669 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756759185323904 33.0 31.0 34.0 30.0 34.0 2 31.896428447437604 33.0 31.0 34.0 30.0 34.0 3 31.922340225966977 34.0 31.0 34.0 30.0 34.0 4 35.511428904016114 37.0 35.0 37.0 33.0 37.0 5 35.50345205444283 37.0 35.0 37.0 33.0 37.0 6 35.57022658838995 37.0 35.0 37.0 33.0 37.0 7 35.510942859418456 37.0 35.0 37.0 33.0 37.0 8 35.503649564762526 37.0 35.0 37.0 33.0 37.0 9 37.14160906435248 39.0 37.0 39.0 34.0 39.0 10-11 37.01912057436118 39.0 37.0 39.0 33.0 39.0 12-13 36.90649768107714 39.0 37.0 39.0 33.0 39.0 14-15 38.06776193967387 40.0 38.0 41.0 33.0 41.0 16-17 37.98140164642502 40.0 38.0 41.0 33.0 41.0 18-19 37.92162808019094 40.0 38.0 41.0 33.0 41.0 20-21 37.82715892345498 40.0 38.0 41.0 33.0 41.0 22-23 37.743915763161496 40.0 37.5 41.0 32.5 41.0 24-25 37.785869493232866 40.0 38.0 41.0 33.0 41.0 26-27 37.69664865539818 40.0 37.0 41.0 32.5 41.0 28-29 37.577439799467214 40.0 37.0 41.0 32.0 41.0 30-31 37.34711359235679 40.0 37.0 41.0 32.0 41.0 32-33 37.210784063455364 39.5 36.5 41.0 31.5 41.0 34-35 37.21131022277822 40.0 37.0 41.0 31.5 41.0 36-37 37.07958557258592 40.0 36.0 41.0 31.0 41.0 38-39 36.94251880797125 40.0 36.0 41.0 31.0 41.0 40-41 36.72103534501144 39.5 36.0 41.0 30.5 41.0 42-43 36.4612968755151 39.0 36.0 41.0 30.0 41.0 44-45 36.220330784565256 39.0 35.0 41.0 30.0 41.0 46-47 35.87373664143183 39.0 35.0 41.0 28.5 41.0 48-49 35.56405256750287 39.0 35.0 40.0 27.0 41.0 50-51 34.68713402606845 38.0 34.0 39.5 25.0 40.5 52-53 34.782810417225235 38.0 34.5 40.0 26.0 40.5 54-55 35.08129912777459 39.0 35.0 40.0 26.0 41.0 56-57 34.86706053005702 38.0 34.5 40.0 25.5 41.0 58-59 34.62738117361974 38.0 34.0 40.0 25.0 41.0 60-61 34.315182854587185 38.0 34.0 40.0 23.5 41.0 62-63 33.95166365247053 38.0 33.5 40.0 23.0 41.0 64-65 33.578419619864114 37.0 33.0 40.0 22.0 41.0 66-67 33.14105869032278 36.5 32.5 39.0 20.5 41.0 68-69 32.63911670000808 36.0 32.0 39.0 18.5 40.0 70-71 32.19506580127778 35.5 31.5 39.0 17.5 40.0 72-73 31.737406820786955 35.0 31.0 38.0 14.5 40.0 74-75 31.205367554451726 35.0 31.0 37.0 9.5 39.0 76-77 30.715130895077678 35.0 30.0 37.0 7.5 39.0 78-79 30.15220577016042 34.0 29.5 36.0 4.5 39.0 80-81 29.58426470000458 34.0 29.0 36.0 2.0 37.5 82-83 29.074638916418124 34.0 29.0 35.0 2.0 37.0 84-85 28.556501371631857 34.0 28.5 35.0 2.0 36.5 86-87 28.025698222319598 33.5 27.0 35.0 2.0 36.0 88-89 27.56037105683192 33.0 26.0 35.0 2.0 36.0 90-91 27.00927598318274 33.0 25.5 35.0 2.0 35.0 92-93 26.386248788899977 32.0 25.0 35.0 2.0 35.0 94-95 25.662240578072154 32.0 22.5 34.0 2.0 35.0 96-97 24.842628620208075 31.5 19.0 34.0 2.0 35.0 98-99 23.80176717763588 31.0 2.0 34.0 2.0 35.0 100-101 20.97386080744669 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14215397313250833 1101 2 0.2287721946816852 1101 3 0.2247180141800733 1101 4 0.1687359867848457 1101 5 0.09413749223107004 1101 6 0.1136531216771175 1101 7 0.17198447689817442 1101 8 0.18995515567685572 1101 9 0.21228162065654743 1101 10-11 0.21991211849344694 1101 12-13 0.13918344617221123 1101 14-15 0.38863640275229017 1101 16-17 0.4225234587896409 1101 18-19 0.38612095946383107 1101 20-21 0.35706794722308643 1101 22-23 0.27419072732119076 1101 24-25 0.23581891720011328 1101 26-27 0.28700550622872356 1101 28-29 0.3459478020316098 1101 30-31 0.37957048416451755 1101 32-33 0.2957811732034301 1101 34-35 0.3170323281301606 1101 36-37 0.3586045897337726 1101 38-39 0.24514677870097046 1101 40-41 0.3927078152194099 1101 42-43 0.3809409968958235 1101 44-45 0.5404708524886317 1101 46-47 0.45275492393965777 1101 48-49 0.6289453750860972 1101 50-51 0.5302104782692041 1101 52-53 0.5228791772210215 1101 54-55 0.579151411177655 1101 56-57 0.40925294956698366 1101 58-59 0.24299693754380058 1101 60-61 0.4149441852485296 1101 62-63 0.3974769214922773 1101 64-65 0.41621540108226185 1101 66-67 0.43048886000322284 1101 68-69 0.3607138028181538 1101 70-71 0.5226661298116682 1101 72-73 0.4660845490503114 1101 74-75 0.38759239734038076 1101 76-77 0.37049738233703167 1101 78-79 0.5514233833965108 1101 80-81 0.6615932279669501 1101 82-83 0.7033569035498175 1101 84-85 0.6770251349261827 1101 86-87 0.780887204048053 1101 88-89 0.7052725866556386 1101 90-91 0.729608258248998 1101 92-93 0.7548511905202346 1101 94-95 0.7886109980300056 1101 96-97 0.9654878038655141 1101 98-99 0.9919820614898285 1101 100-101 1.2737389927264573 1102 1 0.35143779276115694 1102 2 0.4374837577035926 1102 3 0.3651492942665264 1102 4 0.2764332065510331 1102 5 0.28371652749975595 1102 6 0.326665205378859 1102 7 0.2833682081094864 1102 8 0.35683549755719923 1102 9 0.34789906834296147 1102 10-11 0.353326501045764 1102 12-13 0.4626091264944421 1102 14-15 0.5336371395633748 1102 16-17 0.639876587780698 1102 18-19 0.5682385545124689 1102 20-21 0.5790777222501049 1102 22-23 0.6110816301603634 1102 24-25 0.6106052419864412 1102 26-27 0.4102469837805458 1102 28-29 0.5324678432930341 1102 30-31 0.5853311188044614 1102 32-33 0.5634277986086786 1102 34-35 0.6315032977735413 1102 36-37 0.754267228499863 1102 38-39 0.6594581059433295 1102 40-41 0.5841657187462772 1102 42-43 0.5864228713432169 1102 44-45 0.6555079386464016 1102 46-47 0.5785543639632351 1102 48-49 0.6358284186070691 1102 50-51 0.617527503517266 1102 52-53 0.6192368744407872 1102 54-55 0.6226932620471004 1102 56-57 0.6292033376208224 1102 58-59 0.5806565799448222 1102 60-61 0.6989836292017699 1102 62-63 0.7344955382522755 1102 64-65 0.8008725383256063 1102 66-67 0.7912180573703509 1102 68-69 0.8552012279090206 1102 70-71 0.7876909357847524 1102 72-73 0.8102576014130243 1102 74-75 0.8875408205646629 1102 76-77 0.786757574890327 1102 78-79 0.889795276509787 1102 80-81 0.8310032878942515 1102 82-83 0.9187636232668801 1102 84-85 0.938304484567599 1102 86-87 1.0748293889385145 1102 88-89 1.1703686176999426 1102 90-91 1.2202667217522922 1102 92-93 1.178680888918901 1102 94-95 1.355658350422182 1102 96-97 1.562477930597023 1102 98-99 1.519617389125159 1102 100-101 1.6743444058318673 1103 1 0.30160030630394985 1103 2 0.2966166175406748 1103 3 0.20881628194759472 1103 4 0.166376427128057 1103 5 0.2009996421420297 1103 6 0.17022184435803211 1103 7 0.2503599018617493 1103 8 0.27669224487873834 1103 9 0.3528211220326227 1103 10-11 0.340985252700996 1103 12-13 0.2425760720423753 1103 14-15 0.33048317302912267 1103 16-17 0.411319597692021 1103 18-19 0.32920821865113936 1103 20-21 0.41748157843564826 1103 22-23 0.4072690825097922 1103 24-25 0.3778376965428336 1103 26-27 0.43398725303137553 1103 28-29 0.3770672775051409 1103 30-31 0.39411638023057094 1103 32-33 0.40594690899750674 1103 34-35 0.4743083116172855 1103 36-37 0.376749522549062 1103 38-39 0.40651455432019645 1103 40-41 0.5180771288020551 1103 42-43 0.5945620912782132 1103 44-45 0.4986362240282105 1103 46-47 0.49860748181565384 1103 48-49 0.47481311023260986 1103 50-51 0.3549878783015856 1103 52-53 0.4192062777552579 1103 54-55 0.4887181506952274 1103 56-57 0.5420985866076151 1103 58-59 0.5841222815619247 1103 60-61 0.6792492686223355 1103 62-63 0.5956151763085344 1103 64-65 0.6926380172389699 1103 66-67 0.6986864073563126 1103 68-69 0.630677984459723 1103 70-71 0.6902515272537926 1103 72-73 0.4919607834683184 1103 74-75 0.44226800847847514 1103 76-77 0.5778694885374485 1103 78-79 0.6751191499498574 1103 80-81 0.8213906619964142 1103 82-83 0.7725804343900293 1103 84-85 0.7635811086998565 1103 86-87 0.9063657939784413 1103 88-89 1.091607984097763 1103 90-91 1.1325405069134007 1103 92-93 0.8755843538681383 1103 94-95 1.0404575659432318 1103 96-97 1.2741014107944473 1103 98-99 1.4317414511611943 1103 100-101 1.4828939223039157 1104 1 0.22581322285420669 1104 2 0.3340616298940695 1104 3 0.30846444318260424 1104 4 0.2468097270442371 1104 5 0.2550090838564927 1104 6 0.20444497779106996 1104 7 0.282760841924528 1104 8 0.2935432536931941 1104 9 0.35035147137879363 1104 10-11 0.37952128707134847 1104 12-13 0.5328079583035432 1104 14-15 0.5921415127993654 1104 16-17 0.5065637825600717 1104 18-19 0.38574072367740797 1104 20-21 0.5673146981183521 1104 22-23 0.4546231700846377 1104 24-25 0.40165905722344775 1104 26-27 0.43027627166280524 1104 28-29 0.5441232402751481 1104 30-31 0.546689980134154 1104 32-33 0.4961131250804769 1104 34-35 0.373729708728078 1104 36-37 0.49343204181869993 1104 38-39 0.6619388494100704 1104 40-41 0.6852798051870863 1104 42-43 0.6856905911768578 1104 44-45 0.6813054599806847 1104 46-47 0.7057118104322342 1104 48-49 0.7698896272890536 1104 50-51 0.7508434669090747 1104 52-53 0.7625212727426813 1104 54-55 0.759535905336655 1104 56-57 0.7408131904500408 1104 58-59 0.7063901898674629 1104 60-61 0.8523918776800272 1104 62-63 0.9074571049711366 1104 64-65 1.020108626581532 1104 66-67 0.9899862043882095 1104 68-69 1.0086538570034236 1104 70-71 0.845630661256564 1104 72-73 0.8332717766150672 1104 74-75 0.9297590036521619 1104 76-77 0.9103475345654672 1104 78-79 0.9683852860507862 1104 80-81 1.142099605466644 1104 82-83 1.0916685091239273 1104 84-85 1.0673041421553542 1104 86-87 1.1764402224630004 1104 88-89 1.2378831702203286 1104 90-91 1.2023816658859054 1104 92-93 1.316175611448628 1104 94-95 1.5250026475961214 1104 96-97 1.5151898854384989 1104 98-99 1.5275810502101734 1104 100-101 1.3963226177750734 1105 1 0.14433979856922008 1105 2 0.2443376231175982 1105 3 0.11543532014517766 1105 4 0.1824801463117538 1105 5 0.1977116599321178 1105 6 0.13258432771588957 1105 7 0.14157491214587026 1105 8 0.14328409919456675 1105 9 0.13208372574974447 1105 10-11 0.2257952282993827 1105 12-13 0.2542344588572618 1105 14-15 0.2929090304858377 1105 16-17 0.282441540793414 1105 18-19 0.3318075871771953 1105 20-21 0.24775157270621406 1105 22-23 0.39828412947876046 1105 24-25 0.30547065849363975 1105 26-27 0.22430052202196293 1105 28-29 0.31634896558504266 1105 30-31 0.41971925854817727 1105 32-33 0.3157041243705194 1105 34-35 0.4883622908924181 1105 36-37 0.4632784819360509 1105 38-39 0.5496569358260928 1105 40-41 0.4995745969295271 1105 42-43 0.43007564818299926 1105 44-45 0.4689348963665978 1105 46-47 0.6402680429288949 1105 48-49 0.5891634278244382 1105 50-51 0.48543032225415317 1105 52-53 0.5509293422175361 1105 54-55 0.5370685950352438 1105 56-57 0.549623359182192 1105 58-59 0.5538283030287445 1105 60-61 0.6420718582425877 1105 62-63 0.6905807482005244 1105 64-65 0.6149400839559291 1105 66-67 0.7194163069470036 1105 68-69 0.6931788861237322 1105 70-71 0.7061756864907522 1105 72-73 0.8040565803744251 1105 74-75 0.6398546661181967 1105 76-77 0.5678769903819969 1105 78-79 0.5453100605583714 1105 80-81 0.6530560457240036 1105 82-83 0.7814918373998339 1105 84-85 0.7435630084389402 1105 86-87 0.6858629591467995 1105 88-89 0.6532319531093727 1105 90-91 0.6722791583125769 1105 92-93 0.8076464182356169 1105 94-95 1.0865167532296667 1105 96-97 1.2115474397631054 1105 98-99 1.3535711747470742 1105 100-101 1.4755644243916564 1106 1 0.20593750497267038 1106 2 0.3474940520730492 1106 3 0.1978521748332689 1106 4 0.25753179525280245 1106 5 0.23848587379698216 1106 6 0.23367734200721202 1106 7 0.2746992762689402 1106 8 0.2828769514770002 1106 9 0.3726439268314863 1106 10-11 0.2509493062717567 1106 12-13 0.19062043585984867 1106 14-15 0.33333882977918705 1106 16-17 0.32654218168742233 1106 18-19 0.451410354954362 1106 20-21 0.3661483861853583 1106 22-23 0.46311523230259155 1106 24-25 0.34552203088584577 1106 26-27 0.3736129537721524 1106 28-29 0.4877764577616759 1106 30-31 0.4863067114527553 1106 32-33 0.47245662078015016 1106 34-35 0.6910191942192014 1106 36-37 0.4737618554166474 1106 38-39 0.4998882620505327 1106 40-41 0.6323725592413183 1106 42-43 0.560203483682848 1106 44-45 0.42564869712894193 1106 46-47 0.46111561958748837 1106 48-49 0.5715291474649362 1106 50-51 0.5231911602670962 1106 52-53 0.4382696988320447 1106 54-55 0.40782264308523963 1106 56-57 0.3237766164084377 1106 58-59 0.3789120040872831 1106 60-61 0.37100831918446175 1106 62-63 0.4481457896288177 1106 64-65 0.5181678010589579 1106 66-67 0.543311589659389 1106 68-69 0.4912313146994265 1106 70-71 0.43213734007909466 1106 72-73 0.4974260515430906 1106 74-75 0.3947488390326406 1106 76-77 0.5836326458059453 1106 78-79 0.572325174535699 1106 80-81 0.533427106465787 1106 82-83 0.5758079426935296 1106 84-85 0.768070375340006 1106 86-87 0.8210004611414483 1106 88-89 0.8053363626966537 1106 90-91 1.004618558131643 1106 92-93 1.0261988937504434 1106 94-95 1.201241983406252 1106 96-97 1.374652930751747 1106 98-99 1.652421356686549 1106 100-101 1.72983662729078 1107 1 0.262321358865794 1107 2 0.24733519681185 1107 3 0.3070749307359044 1107 4 0.2122708750321607 1107 5 0.29341707398299377 1107 6 0.28178657611641 1107 7 0.24329777368345873 1107 8 0.14461962729805578 1107 9 0.2592658660691214 1107 10-11 0.3246912082524247 1107 12-13 0.40087233361936114 1107 14-15 0.4192290379962884 1107 16-17 0.4293996009161347 1107 18-19 0.4098093386984871 1107 20-21 0.46400842688917976 1107 22-23 0.35670105376662065 1107 24-25 0.3875906324713583 1107 26-27 0.3748221928995008 1107 28-29 0.28603941544410105 1107 30-31 0.3182401838866227 1107 32-33 0.3827160571255348 1107 34-35 0.45046189744710574 1107 36-37 0.4506629584797892 1107 38-39 0.3974648224072439 1107 40-41 0.44185574830837027 1107 42-43 0.5439885417969421 1107 44-45 0.4888347056043969 1107 46-47 0.6128071238509847 1107 48-49 0.5871620628381748 1107 50-51 0.36583833696450796 1107 52-53 0.4131298773648311 1107 54-55 0.4905909230773986 1107 56-57 0.41086760555037216 1107 58-59 0.451432859516963 1107 60-61 0.47924617700900285 1107 62-63 0.43915994286964377 1107 64-65 0.37059873387494946 1107 66-67 0.4546823429889173 1107 68-69 0.4516472107614007 1107 70-71 0.41073462496202495 1107 72-73 0.50016736065486 1107 74-75 0.4634644683603746 1107 76-77 0.47820802019777986 1107 78-79 0.5630891579880526 1107 80-81 0.6571861065041595 1107 82-83 0.6399506363165486 1107 84-85 0.6768595603757319 1107 86-87 0.8082626919861191 1107 88-89 0.9481937001816583 1107 90-91 1.0592731353588931 1107 92-93 1.1198622468453188 1107 94-95 1.2413512906306465 1107 96-97 1.3363492510816997 1107 98-99 1.4519538394766585 1107 100-101 1.3681533859531712 1108 1 0.09541629565335441 1108 2 0.13401862594789193 1108 3 0.20180668577230065 1108 4 0.19807888781017624 1108 5 0.19465580604983046 1108 6 0.22413755789953171 1108 7 0.24796633352067943 1108 8 0.2367145692056809 1108 9 0.18142236684460045 1108 10-11 0.2656383515115621 1108 12-13 0.26926939453425547 1108 14-15 0.2642857552669682 1108 16-17 0.3802248667494368 1108 18-19 0.40177694769764827 1108 20-21 0.3754860894515417 1108 22-23 0.33407292176212167 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-1.3591179318625741 2316 88-89 -1.4738635192205223 2316 90-91 -1.3143362484489067 2316 92-93 -1.5979160466145181 2316 94-95 -1.4043426174812943 2316 96-97 -1.353038614240056 2316 98-99 -1.6269494993176536 2316 100-101 -1.572266669508899 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12794.0 3 2449.0 4 2204.0 5 2476.0 6 2888.0 7 3531.0 8 4197.0 9 5352.0 10 6898.0 11 8895.0 12 12165.0 13 15749.0 14 17935.0 15 22232.0 16 28230.0 17 36315.0 18 31333.0 19 22428.0 20 22315.0 21 23874.0 22 25899.0 23 28353.0 24 32681.0 25 37116.0 26 42899.0 27 50505.0 28 59646.0 29 72457.0 30 88081.0 31 109761.0 32 138444.0 33 180733.0 34 243362.0 35 337707.0 36 490334.0 37 665071.0 38 493456.0 39 46903.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07749132138293 19.241023071468017 15.090740242647138 22.59074536450192 2 14.755174659572265 20.13098078180032 41.704657764139796 23.409186794487617 3 18.806988902834707 24.829739115679097 30.299503509372467 26.06376847211373 4 12.282620462451762 16.669036563707806 38.38694859664059 32.66139437719984 5 13.232145568992443 37.63785130887969 34.76347467374934 14.366528448378526 6 31.099026826293812 36.576885165970374 18.07837070867578 14.245717299060034 7 27.834530803168583 33.154553264680146 21.25343068603782 17.757485246113447 8 25.673144746692483 35.260212089083616 20.274364710556018 18.792278453667887 9 26.014447826497467 17.130142394078455 21.14014064312793 35.71526913629615 10-11 24.252821178144806 27.0658870289242 27.943023339435335 20.738268453495653 12-13 25.158912177235678 24.428015546004296 28.64884164963497 21.764230627125063 14-15 22.274561984154744 25.7494869590928 27.16410746666897 24.811843590083495 16-17 21.26631574623989 29.117927650835103 27.77568844369914 21.84006815922587 18-19 21.162550189298717 27.676168238504694 30.30574256777455 20.855539004422035 20-21 22.03618136601339 26.636593228025145 30.45643728399056 20.8707881219709 22-23 21.696759729150013 26.537429374505944 30.36770926636588 21.398101629978164 24-25 21.458335525554283 26.618545955381578 30.51206189195098 21.411056627113155 26-27 21.283942267419313 26.87833876635643 30.441029169386102 21.39668979683816 28-29 21.118238469377076 27.07634815521141 30.53581273418312 21.26960064122839 30-31 21.390319616762742 26.7299480552966 30.445051133478508 21.434681194462154 32-33 21.22805158357482 26.794751016798646 30.353915359963164 21.62328203966337 34-35 21.586827353850428 26.864714351489816 30.264417630765404 21.28404066389435 36-37 21.550823027436877 26.72855781942921 30.18725899955415 21.533360153579764 38-39 21.29343499582089 27.054646996592417 30.192867454950346 21.459050552636345 40-41 21.571387612919292 26.9097238966431 30.08376829307855 21.435120197359055 42-43 21.33549065217048 26.828922531189146 29.961268225213093 21.874318591427276 44-45 21.502660266359864 27.0402001002021 29.76299046560069 21.69414916783735 46-47 21.605779929643596 27.22181063168324 29.392178129824508 21.78023130884866 48-49 21.67444033054672 27.152713947243534 29.338908019576976 21.833937702632767 50-51 21.706073871920882 27.40050418772808 29.206655582019952 21.686766358331084 52-53 21.730255220166626 27.56572821669848 28.878399345287182 21.825617217847707 54-55 21.662484600399548 27.418522778255983 29.006343977751502 21.912648643592963 56-57 21.73051177495334 27.68707494921528 28.892976409763644 21.68943686606774 58-59 21.818022679913152 27.391649255217626 28.95741572841617 21.83291233645305 60-61 21.78819558594692 27.434501501304055 29.185599170082043 21.591703742666986 62-63 21.658892642481927 27.44815275537417 29.194652398759292 21.698302203384603 64-65 21.580337795285946 27.42451185673435 29.11116501236888 21.883985335610824 66-67 21.58946901926874 27.47063326477089 28.904614529500282 22.035283186460084 68-69 21.50848968953604 27.445719441165462 29.059019102349442 21.986771766949058 70-71 21.69324887307333 27.36687477798642 28.91133674566344 22.028539603276805 72-73 21.718271284274657 27.413253276819184 28.754519397904566 22.113956041001593 74-75 21.449541051143896 27.679283906228513 28.681611199296043 22.18956384333155 76-77 21.59561907266962 27.452807855859014 28.677801645404994 22.27377142606637 78-79 21.612974076951822 27.44287972882311 28.687045168821378 22.25710102540369 80-81 21.539307914186267 27.730001198189274 28.574636963262346 22.15605392436211 82-83 21.74493072713386 27.58735619872955 28.608041839154158 22.059671234982428 84-85 21.71205383135971 27.476593647545382 28.44975496731607 22.361597553778832 86-87 21.492190891240828 27.43140027751239 28.75000054789485 22.32640828335193 88-89 21.68438666066243 27.29469855457099 28.693576237957068 22.32733854680951 90-91 21.71148399706702 27.231755762134256 28.7384720053516 22.318288235447117 92-93 21.720163432957072 27.049340809496925 28.81985964075608 22.410636116789924 94-95 21.496080767817364 27.117316876625097 28.803437848626828 22.58316450693071 96-97 21.411550001862974 27.18902028742405 28.83504203381262 22.56438767690036 98-99 21.40363710114365 27.23575228107869 28.692553668400038 22.668056949377615 100-101 21.642118764901127 26.67741398251601 29.161241877425088 22.519225375157774 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4211.0 1 2384.5 2 568.0 3 593.5 4 538.5 5 531.5 6 815.5 7 1725.0 8 2978.0 9 3603.5 10 3431.0 11 3236.5 12 3552.5 13 3916.5 14 4250.0 15 4651.5 16 4951.0 17 5261.5 18 5643.0 19 6039.5 20 6602.0 21 7680.5 22 9110.5 23 11008.0 24 13518.0 25 17297.0 26 22250.0 27 27968.0 28 33130.0 29 38394.0 30 45522.5 31 52957.0 32 60499.0 33 68685.0 34 77741.5 35 86107.5 36 95348.5 37 103128.5 38 106739.0 39 108667.5 40 110384.0 41 115506.5 42 120328.0 43 123927.0 44 129919.0 45 134692.5 46 135658.0 47 136024.0 48 137852.0 49 134207.0 50 126121.0 51 118233.5 52 110328.5 53 103062.0 54 97789.0 55 90483.0 56 79467.0 57 69544.0 58 61675.5 59 53775.5 60 44241.0 61 35753.5 62 28067.5 63 20857.5 64 15464.5 65 11519.0 66 8212.5 67 6056.0 68 4363.5 69 2951.0 70 2203.5 71 1575.5 72 1163.0 73 953.5 74 710.5 75 469.0 76 307.5 77 212.0 78 139.0 79 85.5 80 65.5 81 46.5 82 30.0 83 22.0 84 20.5 85 16.5 86 10.0 87 10.5 88 8.5 89 6.5 90 6.0 91 5.0 92 3.0 93 1.0 94 0.5 95 0.5 96 1.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31910899214597443 2 0.2848291360688561 3 0.28237849103866214 4 0.2858794125103678 5 0.295477772211961 6 0.19538059246677555 7 0.19564316157715347 8 0.19686848409225044 9 0.19459288513564174 10-11 0.20232408671899182 12-13 0.1924631579070208 14-15 0.19387811366850183 16-17 0.2000047262439868 18-19 0.19316334220136192 20-21 0.1860593890483591 22-23 0.18570929690118854 24-25 0.18907893381770527 26-27 0.18403177202932955 28-29 0.19648921759948235 30-31 0.20176977415263841 32-33 0.20441005242921648 34-35 0.20258665582936977 36-37 0.17867827961217958 38-39 0.17180772122395715 40-41 0.17761341599786912 42-43 0.18808700606738865 44-45 0.19205471706865512 46-47 0.19425738016126995 48-49 0.19282783722699012 50-51 0.19608077676111665 52-53 0.2200766760150995 54-55 0.18420681810291484 56-57 0.17131175734879886 58-59 0.17010102200650062 60-61 0.16466000655255802 62-63 0.1724349696543044 64-65 0.1684526714802392 66-67 0.16709606440995323 68-69 0.1764464421739672 70-71 0.17103460106562215 72-73 0.16784001022269068 74-75 0.17161808797757308 76-77 0.17560038615163834 78-79 0.17908672045054524 80-81 0.17034900394407979 82-83 0.1692841403297693 84-85 0.16263238953352846 86-87 0.15986082670176147 88-89 0.16454330917016782 90-91 0.1718368955695547 92-93 0.1631721149270831 94-95 0.16808799216026987 96-97 0.16692101833636794 98-99 0.169882214414519 100-101 0.1490517316578701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3427669.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.6927749686237 #Duplication Level Percentage of deduplicated Percentage of total 1 70.96573826331914 34.55518723738048 2 14.831827709769424 14.444056980904 3 5.641365801465112 8.240812664592905 4 2.8011122540918736 5.455757146013997 5 1.5608895495646935 3.800202179391502 6 0.9831577457193226 2.8723607322582208 7 0.6529000544656406 2.2254060800368323 8 0.46742040515347805 1.820799728310498 9 0.3169833110750832 1.3891317331489443 >10 1.6025181183396668 14.009286909007546 >50 0.11270139278920924 3.7992750754357463 >100 0.06044602357624841 5.119729599349835 >500 0.002099550481453372 0.7017268935704299 >1k 6.598587203517478E-4 0.5979458540916045 >5k 5.998715639561343E-5 0.2148936372698908 >10k+ 1.1997431279122687E-4 0.7534275492374015 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15040 0.43878215778711427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10754 0.3137409125560257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7357 0.21463566056115685 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4573 0.13341428241758466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9174345597547486E-5 0.0 4 0.0 0.0 0.0 2.9174345597547486E-5 0.0 5 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 6 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 7 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 8 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 9 0.0 0.0 0.0 8.752303679264247E-5 2.9174345597547486E-5 10-11 0.0 0.0 0.0 1.1669738239018994E-4 2.9174345597547486E-5 12-13 0.0 0.0 0.0 1.1669738239018994E-4 2.9174345597547486E-5 14-15 0.0 0.0 0.0 1.1669738239018994E-4 2.9174345597547486E-5 16-17 0.0 0.0 0.0 1.312845551889637E-4 2.9174345597547486E-5 18-19 0.0 0.0 0.0 1.4587172798773745E-4 5.834869119509497E-5 20-21 0.0 0.0 0.0 2.042204191828324E-4 5.834869119509497E-5 22-23 0.0 0.0 0.0 2.9174345597547484E-4 5.834869119509497E-5 24-25 0.0 0.0 0.0 4.667895295607598E-4 5.834869119509497E-5 26-27 0.0 0.0 0.0 6.855971215423659E-4 5.834869119509497E-5 28-29 0.0 0.0 0.0 0.001254496860694542 5.834869119509497E-5 30-31 0.0 0.0 0.0 0.002508993721389084 5.834869119509497E-5 32-33 0.0 0.0 0.0 0.004507436394821087 5.834869119509497E-5 34-35 0.0 0.0 0.0 0.006987255770612624 5.834869119509497E-5 36-37 0.0 0.0 0.0 0.009963039021562467 5.834869119509497E-5 38-39 0.0 0.0 0.0 0.013055519654902502 5.834869119509497E-5 40-41 0.0 0.0 0.0 0.01678983589138858 5.834869119509497E-5 42-43 0.0 0.0 0.0 0.023251953441245348 5.834869119509497E-5 44-45 0.0 0.0 0.0 0.03359425895557593 5.834869119509497E-5 46-47 0.0 0.0 0.0 0.053666208726688605 5.834869119509497E-5 48-49 0.0 0.0 0.0 0.08663321925191728 5.834869119509497E-5 50-51 0.0 0.0 0.0 0.11663903369899487 5.834869119509497E-5 52-53 0.0 0.0 0.0 0.1442817261526711 5.834869119509497E-5 54-55 0.0 1.4587172798773743E-5 0.0 0.17774470055305808 5.834869119509497E-5 56-57 0.0 2.9174345597547486E-5 0.0 0.20856739667686697 5.834869119509497E-5 58-59 0.0 2.9174345597547486E-5 0.0 0.25012625198057337 5.834869119509497E-5 60-61 0.0 2.9174345597547486E-5 0.0 0.3092918248523997 5.834869119509497E-5 62-63 0.0 2.9174345597547486E-5 0.0 0.35785252309951754 5.834869119509497E-5 64-65 0.0 2.9174345597547486E-5 0.0 0.4179225006848678 5.834869119509497E-5 66-67 0.0 2.9174345597547486E-5 0.0 0.5120535267553548 5.834869119509497E-5 68-69 0.0 2.9174345597547486E-5 0.0 0.6188899803335737 5.834869119509497E-5 70-71 0.0 2.9174345597547486E-5 0.0 0.71267091425689 5.834869119509497E-5 72-73 0.0 2.9174345597547486E-5 0.0 0.82163709506373 5.834869119509497E-5 74-75 5.834869119509497E-5 2.9174345597547486E-5 0.0 0.9334040130479343 5.834869119509497E-5 76-77 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.0367249579816487 5.834869119509497E-5 78-79 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.1622038183967005 5.834869119509497E-5 80-81 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.3243694184006682 5.834869119509497E-5 82-83 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.4973732877941248 5.834869119509497E-5 84-85 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.696138104350216 5.834869119509497E-5 86-87 5.834869119509497E-5 2.9174345597547486E-5 0.0 1.9689911715512787 5.834869119509497E-5 88-89 5.834869119509497E-5 2.9174345597547486E-5 0.0 2.330767644133666 5.834869119509497E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16780 0.0 34.577465 1 GGTATCA 11075 0.0 33.70941 1 ATCAACG 20370 0.0 28.241394 3 TCAACGC 20500 0.0 28.087137 4 TATCAAC 20625 0.0 28.007895 2 CAACGCA 20695 0.0 27.799917 5 AACGCAG 21430 0.0 26.865261 6 GTACATG 14200 0.0 26.558903 1 TACATGG 14310 0.0 25.649317 2 ACGCAGA 24010 0.0 23.801088 7 CGCAGAG 24060 0.0 23.671959 8 ACATGGG 14700 0.0 23.610071 3 GGACCGA 795 0.0 22.705294 6 GAGTACT 12825 0.0 22.481543 12-13 GCAGAGT 25545 0.0 21.71907 9 AGTACTT 13535 0.0 20.863558 12-13 GTACTTT 13960 0.0 20.618628 14-15 AGAGTAC 20800 0.0 20.393532 10-11 CATGGGG 7140 0.0 20.243826 4 CATGGGA 7380 0.0 19.26336 4 >>END_MODULE