FastQCFastQC Report
Thu 26 May 2016
SRR937831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427669
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168000.4901290060387978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131140.38259236816623776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91860.2679955386590712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188950.033.6489871
GGTATCA123200.032.360411
ATCAACG230550.027.0803933
TATCAAC231750.027.0016782
TCAACGC232200.026.8879624
CAACGCA234900.026.5384525
AACGCAG242250.025.7630886
GTACATG152300.023.4628471
TACATGG153200.022.6406482
ACGCAGA274400.022.6077377
CGCAGAG274650.022.4833898
GAGTACT148100.021.80980912-13
AGTACTT153400.021.66009512-13
ACATGGG155700.021.178523
GCAGAGT291800.020.7550099
AGAGTAC235850.020.51271410-11
GTACTTT156000.020.490714-15
TACTTTT179050.018.5425414-15
CATGGGG73650.018.1283514
ACTTTTT187600.017.30514316-17
Produced by FastQC (version 0.11.5)