##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937831_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3427669 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.167690345829776 33.0 31.0 34.0 31.0 34.0 2 32.456710668387174 34.0 31.0 34.0 31.0 34.0 3 32.55351785717932 34.0 31.0 34.0 31.0 34.0 4 36.179469487864786 37.0 35.0 37.0 35.0 37.0 5 36.095034555553646 37.0 35.0 37.0 35.0 37.0 6 36.13289789650051 37.0 35.0 37.0 35.0 37.0 7 36.07642774141844 37.0 35.0 37.0 35.0 37.0 8 36.05508641587038 37.0 35.0 37.0 35.0 37.0 9 37.75471523067134 39.0 37.0 39.0 35.0 39.0 10-11 37.671341952796496 39.0 37.0 39.0 35.0 39.0 12-13 37.57117373351978 39.0 37.0 39.0 35.0 39.0 14-15 38.80610890374771 40.0 38.0 41.0 35.0 41.0 16-17 38.7733087704793 40.0 38.0 41.0 35.0 41.0 18-19 38.74876789444955 40.0 38.0 41.0 35.0 41.0 20-21 38.65078060337798 40.0 38.0 41.0 34.0 41.0 22-23 38.59676999150151 40.0 38.0 41.0 34.0 41.0 24-25 38.626759176571596 40.0 38.0 41.0 34.0 41.0 26-27 38.49857891762594 40.0 38.0 41.0 34.0 41.0 28-29 38.348689152890785 40.0 38.0 41.0 34.0 41.0 30-31 38.39727887961177 40.0 38.0 41.0 34.0 41.0 32-33 38.327895138066125 40.0 38.0 41.0 34.0 41.0 34-35 38.19227483750619 40.0 38.0 41.0 34.0 41.0 36-37 38.05104051762291 40.0 38.0 41.0 33.5 41.0 38-39 37.928003549934374 40.0 37.0 41.0 33.0 41.0 40-41 37.75300590576278 40.0 37.0 41.0 33.0 41.0 42-43 37.53778354911165 40.0 37.0 41.0 33.0 41.0 44-45 37.242064359189875 40.0 37.0 41.0 32.5 41.0 46-47 36.87439379356641 40.0 36.5 41.0 31.0 41.0 48-49 36.55709448607786 40.0 36.0 41.0 30.5 41.0 50-51 36.30368480737201 39.0 36.0 41.0 30.0 41.0 52-53 36.07986958484031 39.0 35.5 41.0 29.5 41.0 54-55 35.86057930331079 39.0 35.0 40.0 29.0 41.0 56-57 35.59886996089762 39.0 35.0 40.0 28.0 41.0 58-59 35.34477337222468 38.0 35.0 40.0 28.0 41.0 60-61 35.03177217520128 38.0 34.0 40.0 27.5 41.0 62-63 34.66997469709006 38.0 34.0 40.0 26.5 41.0 64-65 34.26824147839246 37.0 34.0 40.0 26.0 41.0 66-67 33.854963825270175 36.5 33.0 39.0 26.0 41.0 68-69 33.40984704182347 36.0 33.0 39.0 25.0 40.0 70-71 33.01604647356557 35.5 32.5 38.5 24.0 40.0 72-73 32.53467764827934 35.0 32.0 38.0 22.5 40.0 74-75 32.05226452729245 35.0 31.5 37.0 22.0 39.0 76-77 30.811145854515125 34.0 30.0 36.0 20.5 38.5 78-79 31.399905883561104 34.5 31.0 36.0 21.0 39.0 80-81 31.2059522083375 34.0 31.0 36.0 20.0 37.5 82-83 30.824898057542896 34.0 31.0 35.5 19.0 37.0 84-85 30.441425645241708 34.0 31.0 35.0 18.0 37.0 86-87 30.068296851300403 34.0 30.0 35.0 14.0 36.0 88-89 29.642642711417 34.0 30.0 35.0 7.5 36.0 90-91 29.112445659134533 34.0 29.0 35.0 4.0 35.5 92-93 28.6708041237354 34.0 29.0 35.0 2.0 35.0 94-95 28.208712830789672 33.5 29.0 35.0 2.0 35.0 96-97 27.64815928842604 33.0 27.5 35.0 2.0 35.0 98-99 26.778384085511174 33.0 26.0 34.5 2.0 35.0 100-101 24.001660166136226 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24038721601240098 1101 2 0.18723817829018685 1101 3 0.18263928438226884 1101 4 0.0676702804499385 1101 5 0.0033558594504228267 1101 6 0.011266611742279053 1101 7 0.1126501868169143 1101 8 0.14290374703056585 1101 9 0.1702940027122537 1101 10-11 0.1538350708358962 1101 12-13 0.14355897497188153 1101 14-15 0.15885141480616483 1101 16-17 0.18935521226590168 1101 18-19 0.18510451428734598 1101 20-21 0.17776799944406463 1101 22-23 0.10210944661972121 1101 24-25 0.11734070945237107 1101 26-27 -0.053973966750646696 1101 28-29 -0.21796072721621584 1101 30-31 -0.1345415276159514 1101 32-33 -0.09550087154168097 1101 34-35 0.03664405221517342 1101 36-37 3.050800073509663E-4 1101 38-39 -0.03525919681005263 1101 40-41 0.008433910512607667 1101 42-43 0.08467583230594755 1101 44-45 -0.00280503899649176 1101 46-47 -0.2397158407409492 1101 48-49 -0.2615759057453815 1101 50-51 -0.1041452529503033 1101 52-53 -0.19723186768143108 1101 54-55 -0.30686804646601473 1101 56-57 -0.3203456837578287 1101 58-59 -0.28014043511547726 1101 60-61 -0.22375919626770724 1101 62-63 -0.3110335576963408 1101 64-65 -0.2526321181656286 1101 66-67 -0.352600081239153 1101 68-69 -0.34692704112780604 1101 70-71 -0.26445306768247434 1101 72-73 -0.3964090213734579 1101 74-75 -0.25228875497714753 1101 76-77 -0.23520703077516103 1101 78-79 -0.34038254418545577 1101 80-81 -0.3236975365735262 1101 82-83 -0.29388813224324295 1101 84-85 -0.30257510087011497 1101 86-87 -0.39359501374406847 1101 88-89 -0.4323274370304837 1101 90-91 -0.42186684486052073 1101 92-93 -0.4825837761635263 1101 94-95 -0.6621678149156409 1101 96-97 -0.6523480634583585 1101 98-99 -0.6246970121795385 1101 100-101 -0.48895455126952214 1102 1 0.3279719492867912 1102 2 0.2285290713052177 1102 3 0.2228765375505546 1102 4 0.12885998474308735 1102 5 0.14246824770074085 1102 6 0.09783208968706703 1102 7 0.08740703915997727 1102 8 0.08086670999352208 1102 9 0.24815774178289018 1102 10-11 0.21033865664982443 1102 12-13 0.24520451972087187 1102 14-15 0.29854465579251155 1102 16-17 0.322967305791785 1102 18-19 0.312103728355531 1102 20-21 0.27559809080864284 1102 22-23 0.28039808008077216 1102 24-25 0.2818669399268785 1102 26-27 0.2232733070483519 1102 28-29 0.1654069906342599 1102 30-31 0.2315676186656077 1102 32-33 0.2605851879926533 1102 34-35 0.31323433613304275 1102 36-37 0.2218346998128382 1102 38-39 0.24699716432561303 1102 40-41 0.2935761150513656 1102 42-43 0.25739171795287064 1102 44-45 0.24834070223544558 1102 46-47 0.153423220070529 1102 48-49 0.2343360207699945 1102 50-51 0.12175120053235844 1102 52-53 0.24053215628948976 1102 54-55 0.11024807496045952 1102 56-57 0.1064042425610694 1102 58-59 0.09320910806165017 1102 60-61 0.18938796946566328 1102 62-63 0.09623139956862303 1102 64-65 0.13199063456057303 1102 66-67 0.10208849327700875 1102 68-69 0.20623633444936473 1102 70-71 0.1338068301463835 1102 72-73 -0.030830379070685865 1102 74-75 -0.03505719981455968 1102 76-77 0.07323314016500859 1102 78-79 0.2504232815341645 1102 80-81 0.13354286032204143 1102 82-83 0.14787800391944117 1102 84-85 0.21162816074694035 1102 86-87 0.008651032036461004 1102 88-89 0.07551223287815034 1102 90-91 0.15879309226493632 1102 92-93 0.0201952296327228 1102 94-95 -0.0800060112021157 1102 96-97 0.02809574241647894 1102 98-99 0.15338531417864942 1102 100-101 0.18980686930224167 1103 1 0.34372823916280026 1103 2 0.21563181597839076 1103 3 0.24198241211417582 1103 4 0.11383751373470119 1103 5 0.08918838332932921 1103 6 0.08578903362992918 1103 7 0.1283333663539068 1103 8 0.11966431041084746 1103 9 0.17131391529117934 1103 10-11 0.1971783199859658 1103 12-13 0.2232062552050067 1103 14-15 0.33588314541972863 1103 16-17 0.2324748496303215 1103 18-19 0.15123087012351277 1103 20-21 0.1371515998973578 1103 22-23 0.16055913902703622 1103 24-25 0.16335920548603866 1103 26-27 0.16714368745042663 1103 28-29 0.1729754068294369 1103 30-31 0.18834478450967396 1103 32-33 0.30882161510232464 1103 34-35 0.320477223657619 1103 36-37 0.3636367944318337 1103 38-39 0.2986822391620976 1103 40-41 0.2424896819226774 1103 42-43 0.27388985205663374 1103 44-45 0.31622321096303097 1103 46-47 0.30872798312176997 1103 48-49 0.20360541204642146 1103 50-51 0.18539356038950672 1103 52-53 0.1643514251791771 1103 54-55 0.040465813012694696 1103 56-57 0.05076893498913648 1103 58-59 0.13731949689034906 1103 60-61 0.15395449970120723 1103 62-63 0.14310376624732868 1103 64-65 0.15829450479049711 1103 66-67 0.09059341884179162 1103 68-69 0.13454353520375406 1103 70-71 0.07403244308326862 1103 72-73 0.12197874777013595 1103 74-75 0.12914944317729748 1103 76-77 -0.013728157535727092 1103 78-79 0.09581221054985534 1103 80-81 0.10737843072451625 1103 82-83 0.14228031522316442 1103 84-85 0.08805040503890282 1103 86-87 0.1307827181646246 1103 88-89 0.0668388249089702 1103 90-91 0.09624368614158385 1103 92-93 0.1283580240631217 1103 94-95 0.11839819609294011 1103 96-97 0.059401167559929746 1103 98-99 0.2629598196158298 1103 100-101 0.2770706227631763 1104 1 0.326385217595039 1104 2 0.2528895416400161 1104 3 0.19306223801630296 1104 4 0.1404596150708457 1104 5 0.018439551560028633 1104 6 0.13122060790639267 1104 7 0.14173788917344154 1104 8 0.13793486332772176 1104 9 0.22134719548576243 1104 10-11 0.19825324972777736 1104 12-13 0.24326390663673436 1104 14-15 0.28143202211045093 1104 16-17 0.2561397386141664 1104 18-19 0.23048669484234807 1104 20-21 0.20480078555976178 1104 22-23 0.24312226868010356 1104 24-25 0.14947482471679052 1104 26-27 0.17025617313856856 1104 28-29 0.19342321033659715 1104 30-31 0.25327215014259963 1104 32-33 0.3522837688102598 1104 34-35 0.36692158410266984 1104 36-37 0.29730443622189284 1104 38-39 0.19054369083597322 1104 40-41 0.1614078975564297 1104 42-43 0.27069194035460953 1104 44-45 0.21425913166029176 1104 46-47 0.2412376658779678 1104 48-49 0.08588768013962067 1104 50-51 0.18402451863997982 1104 52-53 0.14508931420137117 1104 54-55 0.041468841099536746 1104 56-57 0.2326551276800899 1104 58-59 0.25068649389375963 1104 60-61 0.20312119375898163 1104 62-63 0.11172023460238023 1104 64-65 0.17384255290023987 1104 66-67 0.15023190416837906 1104 68-69 0.19972426857324166 1104 70-71 0.11632876281417737 1104 72-73 0.10698057612650302 1104 74-75 0.062257108030671304 1104 76-77 -0.005661134236849108 1104 78-79 0.06851594560115615 1104 80-81 0.04523947974911735 1104 82-83 -0.007804460251936263 1104 84-85 0.06332941769907308 1104 86-87 0.056376317684733834 1104 88-89 0.054692238662592985 1104 90-91 0.16447065295999863 1104 92-93 0.1716147444711389 1104 94-95 0.33066403636991737 1104 96-97 0.34397045610333166 1104 98-99 0.310157264299491 1104 100-101 0.33020594578649565 1105 1 0.2542628177198907 1105 2 0.19134845853198357 1105 3 0.1623616159293988 1105 4 0.06859819664987299 1105 5 0.006488018129644502 1105 6 0.054791885979611266 1105 7 0.049368723948703064 1105 8 0.0457644435479736 1105 9 0.09834971805037185 1105 10-11 0.12114910916352528 1105 12-13 0.16538241355987537 1105 14-15 0.13784501173026342 1105 16-17 0.12274568888887671 1105 18-19 0.19351504782654416 1105 20-21 0.07524953194384665 1105 22-23 -0.016712770893114737 1105 24-25 0.04160970423505006 1105 26-27 -0.012692039672039357 1105 28-29 -0.006621379330894683 1105 30-31 -0.0028003854565668007 1105 32-33 0.021835872728857453 1105 34-35 0.041751754071000846 1105 36-37 0.10359895545768438 1105 38-39 -0.02101033463008406 1105 40-41 0.11145317019796153 1105 42-43 0.1569208445671606 1105 44-45 0.05767337522045324 1105 46-47 0.002608415260986874 1105 48-49 -0.07574529712520217 1105 50-51 0.0606827369178049 1105 52-53 0.13536730683139808 1105 54-55 -0.020085175829336777 1105 56-57 0.029076702283568068 1105 58-59 0.01112105661450613 1105 60-61 -0.11837662635863211 1105 62-63 -0.20405375084822452 1105 64-65 -0.11627107349043087 1105 66-67 0.08185719626783339 1105 68-69 0.034967240002039546 1105 70-71 -0.047617746317698106 1105 72-73 -0.10623640574975468 1105 74-75 -0.16233083665799342 1105 76-77 -0.20202368280910932 1105 78-79 -0.16371580183231416 1105 80-81 -0.24459860930318555 1105 82-83 -0.2313063908487969 1105 84-85 -0.23785231520412253 1105 86-87 -0.3461281139630543 1105 88-89 -0.2381472224845531 1105 90-91 -0.11801679995321024 1105 92-93 0.025907868049483085 1105 94-95 -0.13324321882913637 1105 96-97 -0.022190408528143024 1105 98-99 0.10132147550374881 1105 100-101 0.03375530210054123 1106 1 0.20491421210885363 1106 2 0.22141048388250795 1106 3 0.2372962878863092 1106 4 0.10745945537731671 1106 5 0.1177625862101408 1106 6 0.08963269662163498 1106 7 0.09748334952593041 1106 8 0.039578861681782485 1106 9 0.07648953818130622 1106 10-11 0.039422725958367266 1106 12-13 -0.032232347324971045 1106 14-15 0.13127342700788347 1106 16-17 0.1604326216061267 1106 18-19 0.16672557016057254 1106 20-21 0.26869921287428156 1106 22-23 0.3061590691654388 1106 24-25 0.2640977029686482 1106 26-27 0.26945067140095347 1106 28-29 0.2718455101430166 1106 30-31 0.24089959867101385 1106 32-33 0.21328544854954146 1106 34-35 -0.0914793091671342 1106 36-37 0.2375544495296893 1106 38-39 0.2788147084892856 1106 40-41 0.06913049469697796 1106 42-43 0.07660051623479802 1106 44-45 0.08047429798237005 1106 46-47 0.10051830992448885 1106 48-49 0.07997226025127446 1106 50-51 0.23261887765637113 1106 52-53 0.20683356882551607 1106 54-55 0.1388511558444918 1106 56-57 0.13732231877240508 1106 58-59 0.17168129160262424 1106 60-61 0.18061898213042582 1106 62-63 0.04334732572147715 1106 64-65 -0.14894600864978003 1106 66-67 -0.13009286091737948 1106 68-69 0.19323376188850006 1106 70-71 0.1801929700879228 1106 72-73 0.1250973416651462 1106 74-75 0.17777857920514606 1106 76-77 0.14075543603632568 1106 78-79 0.021649487409174384 1106 80-81 -0.04117404451796247 1106 82-83 -0.2679670730407686 1106 84-85 -0.3390435520174577 1106 86-87 -0.09255035594986438 1106 88-89 -0.0015813509858872976 1106 90-91 -0.20159844448431485 1106 92-93 -0.08195132175351105 1106 94-95 -0.0616553177246999 1106 96-97 0.018520018973990915 1106 98-99 0.17836215700487656 1106 100-101 -0.10060018120618608 1107 1 0.28836544937925623 1107 2 0.20039871937430576 1107 3 0.1563433425131322 1107 4 0.053582521883313916 1107 5 0.10349399593251007 1107 6 0.08881605433048634 1107 7 0.12310909188931163 1107 8 0.13029007503307355 1107 9 0.1918284259992049 1107 10-11 0.1664792896484073 1107 12-13 0.1512021665463621 1107 14-15 0.19872730823292528 1107 16-17 0.13076601556604572 1107 18-19 0.20784134671779242 1107 20-21 0.21971810270851933 1107 22-23 0.24301784942678495 1107 24-25 0.15152867779180923 1107 26-27 0.21479745656873916 1107 28-29 0.24162826944255045 1107 30-31 0.22257101441970661 1107 32-33 0.15077944549641842 1107 34-35 0.16123815803150165 1107 36-37 0.1788974395190408 1107 38-39 0.1534888973930819 1107 40-41 0.24373129592150633 1107 42-43 0.29828268128033386 1107 44-45 0.3214944117014866 1107 46-47 0.21928208856345321 1107 48-49 0.14313925196268684 1107 50-51 0.23859649940964545 1107 52-53 0.3495534179448043 1107 54-55 0.17461436613765358 1107 56-57 0.19197644186797902 1107 58-59 0.18958929315302697 1107 60-61 0.17820958650929697 1107 62-63 0.17527876591608305 1107 64-65 0.2435262819381805 1107 66-67 0.13205595621718658 1107 68-69 0.13035315696694028 1107 70-71 0.15901675314559327 1107 72-73 0.2928490424450487 1107 74-75 0.08139492058865017 1107 76-77 0.13915405420445737 1107 78-79 0.11864349900179505 1107 80-81 0.03520375125856745 1107 82-83 -0.0038635596283320695 1107 84-85 0.12118518502644804 1107 86-87 0.07811363451881803 1107 88-89 0.1975511326216619 1107 90-91 0.08844081292864558 1107 92-93 -0.041259477118313015 1107 94-95 -0.1278978026969142 1107 96-97 0.09319277736299725 1107 98-99 0.14011359756732844 1107 100-101 0.17001688610680077 1108 1 0.18401338276908064 1108 2 0.11708416832416191 1108 3 0.018544078569647127 1108 4 0.019468148593510648 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0.1006101235631256 2316 94-95 0.06516499209296711 2316 96-97 0.17183719435586298 2316 98-99 0.10352502894170357 2316 100-101 0.3961710450903482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 27.0 5 77.0 6 118.0 7 280.0 8 705.0 9 1453.0 10 2475.0 11 4105.0 12 6834.0 13 9587.0 14 12407.0 15 17405.0 16 24254.0 17 34912.0 18 31013.0 19 18277.0 20 15394.0 21 15327.0 22 16060.0 23 17790.0 24 20357.0 25 24227.0 26 29879.0 27 37034.0 28 46218.0 29 58354.0 30 73776.0 31 94590.0 32 121473.0 33 161469.0 34 226033.0 35 326753.0 36 510503.0 37 789142.0 38 629603.0 39 49741.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21964534626025 20.14160238174926 15.479706955901865 22.159045316088623 2 15.262267155900993 21.0946564560347 40.35363391272611 23.289442475338195 3 19.107533428694545 25.07322031386344 30.340677585846244 25.47856867159577 4 12.827055354528106 17.43015442856355 37.52366404107281 32.219126175835534 5 13.33227098703062 38.142026361364685 34.24969457543797 14.276008076166727 6 31.141192454697347 37.26984723437415 17.4153046866544 14.173655624274106 7 27.902519175568003 33.5970596927533 20.755008724588052 17.74541240709065 8 25.984714393367618 35.72118544701953 19.711354859526985 18.58274530008586 9 26.11836207054999 17.293035004255078 20.699052329731956 35.889550595462985 10-11 24.50586681502794 27.534659851928527 27.34415137517654 20.615321957866996 12-13 25.390152095897307 24.859728175428366 28.207200408806138 21.542919319868194 14-15 22.32892090805734 26.23097504455652 26.838632901835034 24.601471145551102 16-17 21.297505680974446 29.600801594319638 27.405257042030605 21.69643568267531 18-19 21.225094955201335 28.00235378620281 30.106713921326712 20.665837337269146 20-21 22.060998305262263 26.907892798283616 30.390040578597294 20.641068317856828 22-23 21.818544112676662 26.8202821847106 30.199391798779835 21.161781903832903 24-25 21.5980360966005 26.938043947429495 30.417411160692993 21.046508795277017 26-27 21.39265424845611 27.217382869054468 30.3929029627636 20.997059919725817 28-29 21.213880083847656 27.422778613304473 30.375957836940525 20.98738346590735 30-31 21.439205477541734 27.140324809659276 30.338387399716833 21.082082313082157 32-33 21.393051662806414 27.138603523269023 30.221573319944255 21.24677149398031 34-35 21.759145938537237 27.155787212825977 30.108654015308943 20.97641283332784 36-37 21.732042971477117 27.034669917077757 30.08115719458326 21.152129916861867 38-39 21.44689291760669 27.393412257718 30.07005635608339 21.08963846859192 40-41 21.776313994856825 27.242875108109832 29.90484584402397 21.075965053009373 42-43 21.557990598189225 27.26643292708279 29.72463749721539 21.450938977512596 44-45 21.74073692646519 27.42483302792656 29.557784021736055 21.2766460238722 46-47 21.88716296701928 27.690975995640187 29.029071943644503 21.392789093696035 48-49 21.921399061578 27.71212739619841 28.95079717440628 21.415676367817312 50-51 21.987106102458 27.945617836966708 28.729839062592994 21.3374369979823 52-53 22.04530250733078 28.1131141892639 28.369804669003923 21.4717786344014 54-55 21.90464437363819 28.065590452505035 28.499238621294072 21.5305265525627 56-57 21.972745909829683 28.28986696206664 28.385062851751435 21.35232427635224 58-59 22.052785726976552 27.970203657354315 28.412005359910776 21.56500525575836 60-61 22.025172208868476 28.005329569453757 28.79273056995877 21.176767651718997 62-63 21.830783544152016 28.032038682848317 28.76572971310824 21.37144805989143 64-65 21.83104929779528 27.958698456399738 28.6601519370966 21.550100308708384 66-67 21.865034225883537 28.04443777972727 28.473942495614367 21.616585498774825 68-69 21.800602683631354 28.056793115087835 28.5270689789475 21.615535222333314 70-71 22.012539717224737 28.017918299579105 28.29500164689181 21.67454033630435 72-73 21.98787280802201 28.072459738673714 28.16672204929939 21.77294540400488 74-75 21.8200245764759 28.455964813394996 27.916107977785483 21.807902632343623 76-77 21.85979743084878 28.175007563449096 28.082058098375313 21.883136907326815 78-79 21.863896426405233 28.191476481538913 28.026626841739972 21.918000250315885 80-81 21.846377231873905 28.441996587185052 27.916902127947594 21.79472405299345 82-83 21.941585498135453 28.415978098062748 27.938740393541682 21.703696010260114 84-85 21.885877478763252 28.28123327032643 27.79988546145235 22.03300378945796 86-87 21.677005568507344 28.247666271159787 28.076296165119796 21.999031995213073 88-89 21.873042583750067 28.1559567157739 27.963770714150055 22.007229986325985 90-91 21.923543202438076 28.116196592356363 27.97089249685247 21.98936770835309 92-93 21.82257236562545 28.029591451656643 28.03767288095515 22.110163301762757 94-95 21.72973819817491 28.061096331063474 27.98439697648752 22.224768494274098 96-97 21.603632089329512 28.163935899294827 28.03435804332332 22.19807396805234 98-99 21.595158159919887 28.184040103599507 27.790647890775606 22.430153845705004 100-101 21.568245545156532 28.229257259340994 27.94678683878381 22.255710356718662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 613.0 1 433.0 2 338.0 3 463.5 4 437.5 5 449.0 6 826.5 7 1763.0 8 2992.0 9 3686.0 10 3630.5 11 3642.0 12 4029.0 13 4444.5 14 4856.0 15 5342.0 16 5732.0 17 6064.5 18 6482.0 19 6872.0 20 7437.0 21 8410.5 22 9770.0 23 11781.0 24 14318.5 25 17927.5 26 22774.0 27 28228.5 28 33263.5 29 38834.5 30 45999.5 31 53313.5 32 60708.0 33 68272.0 34 76830.5 35 85097.0 36 94748.5 37 103234.0 38 106576.5 39 108136.0 40 110176.5 41 115128.0 42 119498.5 43 123312.0 44 129705.5 45 133942.5 46 133551.0 47 134624.0 48 138701.5 49 135174.0 50 127128.0 51 119371.5 52 110647.5 53 103078.0 54 97945.0 55 90705.0 56 79200.0 57 68990.5 58 61502.0 59 53853.0 60 43542.5 61 34924.5 62 28130.0 63 21161.0 64 15233.0 65 10991.5 66 7889.0 67 6065.5 68 4502.5 69 2937.5 70 2123.0 71 1499.0 72 1124.0 73 880.0 74 662.5 75 496.5 76 322.0 77 171.0 78 105.0 79 82.0 80 53.5 81 27.0 82 18.5 83 18.0 84 12.5 85 6.5 86 3.5 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017738002123308873 2 0.0 3 0.0 4 0.0 5 0.03766408016643381 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037197290636873043 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.980740847497235E-4 24-25 1.6045890078651118E-4 26-27 0.0012107353422982207 28-29 7.001842943411397E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.980740847497235E-4 42-43 0.010225608131940395 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.917434559754749E-5 52-53 0.0 54-55 1.6045890078651118E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.4587172798773745E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.917434559754749E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.877073311337821E-4 84-85 5.834869119509498E-5 86-87 0.0 88-89 0.0 90-91 0.01091120525348276 92-93 0.0016775248718589805 94-95 0.0 96-97 0.0 98-99 0.0013566070702859582 100-101 0.017052405001766507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3427669.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.601210183395125 #Duplication Level Percentage of deduplicated Percentage of total 1 69.28617589639975 32.288196457518104 2 15.360697745668922 14.316542084190424 3 5.979079864684811 8.358970724324477 4 3.040977891749772 5.668529995859557 5 1.7262775271732547 4.022331093933622 6 1.0801240185771355 3.020105184830788 7 0.6995418929471127 2.281964914972294 8 0.5080247493605857 1.893965449865543 9 0.3672444383503789 1.540263173422401 >10 1.757740069406422 14.538107149291998 >50 0.12587971405586176 4.05495171212481 >100 0.06415062559647791 5.317744213222868 >500 0.00314274310172903 0.9559159084475407 >1k 7.542583422139039E-4 0.5971289003795738 >5k 6.285486185115866E-5 0.2690682860240736 >10k+ 1.257097237023173E-4 0.8762147515920029 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16800 0.4901290060387978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13114 0.38259236816623776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9186 0.2679955386590712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.9174345597547486E-5 0.0 6 0.0 0.0 0.0 2.9174345597547486E-5 0.0 7 0.0 0.0 0.0 2.9174345597547486E-5 0.0 8 0.0 0.0 0.0 2.9174345597547486E-5 0.0 9 0.0 0.0 0.0 2.9174345597547486E-5 0.0 10-11 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 12-13 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 14-15 0.0 0.0 0.0 5.834869119509497E-5 2.9174345597547486E-5 16-17 0.0 0.0 0.0 8.752303679264246E-5 2.9174345597547486E-5 18-19 0.0 0.0 0.0 1.1669738239018994E-4 2.9174345597547486E-5 20-21 0.0 0.0 0.0 2.479819375791537E-4 2.9174345597547486E-5 22-23 0.0 0.0 0.0 3.500921471705699E-4 2.9174345597547486E-5 24-25 0.0 0.0 0.0 5.251382207558548E-4 2.9174345597547486E-5 26-27 0.0 0.0 0.0 7.43945812737461E-4 2.9174345597547486E-5 28-29 0.0 0.0 0.0 0.0012836712062920894 2.9174345597547486E-5 30-31 0.0 0.0 0.0 0.002567342412584179 2.9174345597547486E-5 32-33 0.0 0.0 0.0 0.004580372258814955 2.9174345597547486E-5 34-35 0.0 0.0 0.0 0.007074778807405266 2.9174345597547486E-5 36-37 0.0 0.0 0.0 0.010123497922348979 2.9174345597547486E-5 38-39 0.0 0.0 0.0 0.01336185028367675 2.9174345597547486E-5 40-41 0.0 0.0 0.0 0.017081579347364054 2.9174345597547486E-5 42-43 0.0 0.0 0.0 0.023572871242818372 2.9174345597547486E-5 44-45 0.0 0.0 0.0 0.03407563565793546 2.9174345597547486E-5 46-47 0.0 0.0 0.0 0.05424969563863955 2.9174345597547486E-5 48-49 0.0 0.0 0.0 0.08727505485506332 2.9174345597547486E-5 50-51 0.0 0.0 0.0 0.11720793343814703 2.9174345597547486E-5 52-53 0.0 0.0 0.0 0.14482145154622572 4.376151839632123E-5 54-55 0.0 0.0 0.0 0.17870745395777715 5.834869119509497E-5 56-57 0.0 0.0 0.0 0.20976354484636645 5.834869119509497E-5 58-59 0.0 0.0 0.0 0.25164331795164585 5.834869119509497E-5 60-61 0.0 0.0 0.0 0.3109985240698562 5.834869119509497E-5 62-63 0.0 0.0 0.0 0.35951546079857766 5.834869119509497E-5 64-65 0.0 0.0 0.0 0.41992094335829977 5.834869119509497E-5 66-67 0.0 0.0 0.0 0.5146208691679389 5.834869119509497E-5 68-69 0.0 0.0 0.0 0.6223033787684866 5.834869119509497E-5 70-71 0.0 0.0 0.0 0.7167844969861442 5.834869119509497E-5 72-73 0.0 0.0 0.0 0.8268447157528922 5.834869119509497E-5 74-75 0.0 0.0 0.0 0.9398953049433887 5.834869119509497E-5 76-77 0.0 0.0 0.0 1.0445290954289925 5.834869119509497E-5 78-79 0.0 0.0 0.0 1.1707956631751784 5.834869119509497E-5 80-81 0.0 0.0 0.0 1.3345366778414136 5.834869119509497E-5 82-83 0.0 0.0 0.0 1.5097869718458812 5.834869119509497E-5 84-85 0.0 0.0 0.0 1.7106669284577944 5.834869119509497E-5 86-87 0.0 0.0 0.0 1.9872251375497458 5.834869119509497E-5 88-89 0.0 0.0 0.0 2.355069874016423 5.834869119509497E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18895 0.0 33.648987 1 GGTATCA 12320 0.0 32.36041 1 ATCAACG 23055 0.0 27.080393 3 TATCAAC 23175 0.0 27.001678 2 TCAACGC 23220 0.0 26.887962 4 CAACGCA 23490 0.0 26.538452 5 AACGCAG 24225 0.0 25.763088 6 GTACATG 15230 0.0 23.462847 1 TACATGG 15320 0.0 22.640648 2 ACGCAGA 27440 0.0 22.607737 7 CGCAGAG 27465 0.0 22.483389 8 GAGTACT 14810 0.0 21.809809 12-13 AGTACTT 15340 0.0 21.660095 12-13 ACATGGG 15570 0.0 21.17852 3 GCAGAGT 29180 0.0 20.755009 9 AGAGTAC 23585 0.0 20.512714 10-11 GTACTTT 15600 0.0 20.4907 14-15 TACTTTT 17905 0.0 18.54254 14-15 CATGGGG 7365 0.0 18.128351 4 ACTTTTT 18760 0.0 17.305143 16-17 >>END_MODULE