FastQCFastQC Report
Thu 26 May 2016
SRR937830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385229
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149500.4416244809435344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107090.3163449208310575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73510.21714926818835592No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44630.13183746210374542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103350.036.9923821
GTATCAA163600.035.9835551
TATCAAC199150.029.3587172
ATCAACG197700.029.333173
TCAACGC199800.029.0508064
CAACGCA202000.028.6852195
AACGCAG207200.028.0650566
GTACATG147100.025.3437041
ACGCAGA233450.024.6654727
TACATGG147900.024.5548572
CGCAGAG233850.024.3998038
ACATGGG149950.023.0776043
GAGTACT124450.022.4427612-13
GCAGAGT249800.022.3853919
GGACCGA8150.022.151356
AGAGTAC206150.020.7722110-11
AGTACTT132500.020.52359612-13
GTACTTT135050.020.45186414-15
CATGGGG72950.020.1359064
AGGACCG10450.019.559515
Produced by FastQC (version 0.11.5)