##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3385229 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.717161527329466 33.0 31.0 34.0 30.0 34.0 2 31.84049232710697 33.0 31.0 34.0 30.0 34.0 3 31.895597314096033 33.0 31.0 34.0 30.0 34.0 4 35.47583457426366 37.0 35.0 37.0 33.0 37.0 5 35.46086631066908 37.0 35.0 37.0 33.0 37.0 6 35.54072855927915 37.0 35.0 37.0 33.0 37.0 7 35.4785354255207 37.0 35.0 37.0 33.0 37.0 8 35.46978919299108 37.0 35.0 37.0 33.0 37.0 9 37.08681657873071 39.0 37.0 39.0 33.0 39.0 10-11 36.975478320669 39.0 37.0 39.0 33.0 39.0 12-13 36.85878001163289 39.0 37.0 39.0 33.0 39.0 14-15 38.00711635756399 40.0 38.0 41.0 33.0 41.0 16-17 37.94972998872454 40.0 38.0 41.0 33.0 41.0 18-19 37.904945130743 40.0 38.0 41.0 33.0 41.0 20-21 37.73911750135663 40.0 38.0 41.0 32.0 41.0 22-23 37.60306407631507 40.0 37.0 41.0 32.0 41.0 24-25 37.462424255493495 40.0 37.0 41.0 32.0 41.0 26-27 37.55968562244976 40.0 37.0 41.0 32.0 41.0 28-29 37.503925436063554 40.0 37.0 41.0 32.0 41.0 30-31 37.30670967902024 40.0 37.0 41.0 32.0 41.0 32-33 37.07586045139044 39.0 36.0 41.0 31.0 41.0 34-35 36.824301841913794 39.0 36.0 41.0 30.5 41.0 36-37 36.85440024884579 39.0 36.0 41.0 31.0 41.0 38-39 36.81658803584632 39.0 36.0 41.0 30.5 41.0 40-41 36.634086054444175 39.0 36.0 41.0 30.0 41.0 42-43 36.4029092271158 39.0 35.5 41.0 30.0 41.0 44-45 36.174605617522474 39.0 35.0 41.0 30.0 41.0 46-47 35.82484995845185 39.0 35.0 41.0 28.5 41.0 48-49 35.507089328373354 39.0 35.0 40.0 27.0 41.0 50-51 34.62405645231091 38.0 34.0 39.5 25.0 40.5 52-53 34.73566514997951 38.0 34.5 39.5 26.0 40.5 54-55 35.055201287711995 39.0 35.0 40.0 26.0 41.0 56-57 34.79592163484361 38.0 34.0 40.0 25.0 41.0 58-59 34.575501096085375 38.0 34.0 40.0 24.0 41.0 60-61 34.28137948717797 38.0 34.0 40.0 23.5 41.0 62-63 33.92834221850279 38.0 33.5 40.0 22.5 41.0 64-65 33.582374043233116 37.0 33.0 40.0 22.0 41.0 66-67 33.18238899052324 36.5 32.5 39.0 20.5 41.0 68-69 32.70243756035411 36.0 32.0 39.0 19.0 40.5 70-71 32.26744542245148 35.5 31.5 39.0 18.0 40.0 72-73 31.798722479335964 35.0 31.0 38.0 14.5 40.0 74-75 31.30900848952907 35.0 31.0 37.0 9.5 39.5 76-77 30.842142289339954 35.0 30.5 37.0 7.5 39.0 78-79 30.309239493103718 34.0 30.0 36.0 4.5 39.0 80-81 29.774528399703534 34.0 29.0 36.0 2.0 37.5 82-83 29.25530680494584 34.0 29.0 35.0 2.0 37.0 84-85 28.776358556540785 34.0 29.0 35.0 2.0 36.5 86-87 28.255846502555663 34.0 27.5 35.0 2.0 36.0 88-89 27.77012072152283 33.0 26.5 35.0 2.0 36.0 90-91 27.25738140610281 33.0 26.0 35.0 2.0 35.0 92-93 26.695624875008455 33.0 25.0 35.0 2.0 35.0 94-95 26.065816522309127 32.0 24.5 34.5 2.0 35.0 96-97 25.298870179831262 32.0 21.5 34.0 2.0 35.0 98-99 24.35886745032611 31.5 10.0 34.0 2.0 35.0 100-101 21.82241629739081 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27450337355601206 1101 2 0.3991550058081792 1101 3 0.3703564981203584 1101 4 0.2895284032693013 1101 5 0.3050601009150995 1101 6 0.2750042852772765 1101 7 0.25864911396723755 1101 8 0.36874558071021823 1101 9 0.429870800196511 1101 10-11 0.4039935729376296 1101 12-13 0.46129977063009164 1101 14-15 0.659487099431125 1101 16-17 0.5925081950579525 1101 18-19 0.5648479670602384 1101 20-21 0.67742589581092 1101 22-23 0.7048659241370459 1101 24-25 0.6097991257363304 1101 26-27 0.6632978263200755 1101 28-29 0.6025541000660084 1101 30-31 0.6138498862968405 1101 32-33 0.6519594413229157 1101 34-35 0.6535744964716841 1101 36-37 0.6499444802649847 1101 38-39 0.7813158827293236 1101 40-41 0.8490604454505828 1101 42-43 0.8578556473940395 1101 44-45 0.9186984550988413 1101 46-47 1.0182040141017978 1101 48-49 1.0771763106153642 1101 50-51 0.973538951096252 1101 52-53 0.8925173173270267 1101 54-55 1.085513092079232 1101 56-57 1.2302045502004049 1101 58-59 1.1523234352362053 1101 60-61 1.226793922756137 1101 62-63 1.1281727572078637 1101 64-65 1.231187222424012 1101 66-67 1.2137053937312317 1101 68-69 1.2546542158393166 1101 70-71 1.2695760751516616 1101 72-73 1.264249179668873 1101 74-75 1.1758643617507225 1101 76-77 1.1387776320259988 1101 78-79 1.2382944072272366 1101 80-81 1.3995840032277584 1101 82-83 1.3535866118518491 1101 84-85 1.5372378129994324 1101 86-87 1.6511024507050287 1101 88-89 1.4920444917243465 1101 90-91 1.6251279542445864 1101 92-93 1.7791695704406436 1101 94-95 1.8630138237688918 1101 96-97 2.011803231221972 1101 98-99 2.4026485081170996 1101 100-101 2.7116571979458506 1102 1 0.34684267213453523 1102 2 0.37832576748143154 1102 3 0.36660686915124074 1102 4 0.2709351903909152 1102 5 0.17621461133453664 1102 6 0.17679912311906065 1102 7 0.26446533938555916 1102 8 0.21466108245127913 1102 9 0.2353583722311683 1102 10-11 0.26004780479538425 1102 12-13 0.43292810722525843 1102 14-15 0.5767504323473602 1102 16-17 0.5838531025300426 1102 18-19 0.4134842539411139 1102 20-21 0.48875812040477484 1102 22-23 0.5650120040059718 1102 24-25 0.6161514173809763 1102 26-27 0.5726194583619133 1102 28-29 0.45154730549340627 1102 30-31 0.4593521416381634 1102 32-33 0.4016556313166362 1102 34-35 0.5366536660590242 1102 36-37 0.5658994762944971 1102 38-39 0.690725711797981 1102 40-41 0.7595339841153361 1102 42-43 0.7289970780891153 1102 44-45 0.8164498763546462 1102 46-47 0.7637915103712416 1102 48-49 0.7604253141468931 1102 50-51 0.6437648820749899 1102 52-53 0.6829342122814452 1102 54-55 0.7270715878056464 1102 56-57 0.7859739032318771 1102 58-59 0.8250579601607768 1102 60-61 0.8555561151447151 1102 62-63 0.9333643954170228 1102 64-65 0.8135699270599446 1102 66-67 0.8241525704747517 1102 68-69 0.8177661535782121 1102 70-71 1.0314209803965468 1102 72-73 0.8262907667132211 1102 74-75 0.784246885781176 1102 76-77 0.8914816726889576 1102 78-79 0.8830894260794508 1102 80-81 0.9103731155518204 1102 82-83 0.8367085841285018 1102 84-85 1.0584576208044219 1102 86-87 0.9627191491801312 1102 88-89 1.0062680643032529 1102 90-91 1.3742922489457605 1102 92-93 1.4462186671905144 1102 94-95 1.4162696830197206 1102 96-97 1.4553670957095513 1102 98-99 1.6409900800569979 1102 100-101 1.8760339409811024 1103 1 0.22851586236729204 1103 2 0.29466207704669145 1103 3 0.2658853302396764 1103 4 0.17954351176824446 1103 5 0.1396320313453998 1103 6 0.23826643796354574 1103 7 0.19787621261479416 1103 8 0.12380986154614959 1103 9 0.1382891017097947 1103 10-11 0.2923519933426917 1103 12-13 0.3152114017886447 1103 14-15 0.44081486939379033 1103 16-17 0.4046245084165676 1103 18-19 0.3678664188364351 1103 20-21 0.3734282043985857 1103 22-23 0.3973370865372132 1103 24-25 0.3744491148639497 1103 26-27 0.16759793755846886 1103 28-29 0.3426429860104605 1103 30-31 0.2904932317469502 1103 32-33 0.3420938478061686 1103 34-35 0.3718328667944846 1103 36-37 0.34143471051605445 1103 38-39 0.5074478635997437 1103 40-41 0.5747197316324346 1103 42-43 0.6228103694487572 1103 44-45 0.3833231613068051 1103 46-47 0.5717486359162294 1103 48-49 0.5183775644289597 1103 50-51 0.4450317539670152 1103 52-53 0.4253099975470107 1103 54-55 0.4783899981913464 1103 56-57 0.7338113758955842 1103 58-59 0.7201402128108256 1103 60-61 0.7381110314991304 1103 62-63 0.6235045244025486 1103 64-65 0.7887590176144741 1103 66-67 0.8891802201801511 1103 68-69 0.8540715400472649 1103 70-71 0.9309257929775043 1103 72-73 0.9187584855080573 1103 74-75 0.9992250863912808 1103 76-77 0.7459091078827846 1103 78-79 0.8180938138160769 1103 80-81 1.0265833647473954 1103 82-83 0.7915155933636413 1103 84-85 0.9837240190994727 1103 86-87 0.9879117220653093 1103 88-89 0.9496147233552215 1103 90-91 0.9473863693786839 1103 92-93 1.2698365301538388 1103 94-95 1.159743098907505 1103 96-97 1.159576537004419 1103 98-99 1.2976761549131801 1103 100-101 1.5597881720128477 1104 1 0.15514728168382774 1104 2 0.21291753002899938 1104 3 0.3038095473529978 1104 4 0.2805059824622447 1104 5 0.3140736407207214 1104 6 0.3093192906764983 1104 7 0.3376356411497454 1104 8 0.299817252844619 1104 9 0.38159071607904593 1104 10-11 0.35439757815085926 1104 12-13 0.44295115647653205 1104 14-15 0.5577981951597693 1104 16-17 0.5147872528699722 1104 18-19 0.5154874879733455 1104 20-21 0.6493732228451705 1104 22-23 0.5097622908855328 1104 24-25 0.5342944586009182 1104 26-27 0.42639855100133417 1104 28-29 0.30138906492388173 1104 30-31 0.38487343693643794 1104 32-33 0.3609557096952756 1104 34-35 0.37102340597351713 1104 36-37 0.3414144729258908 1104 38-39 0.4260846218749421 1104 40-41 0.49760424456199104 1104 42-43 0.4455203085614201 1104 44-45 0.5893851282088889 1104 46-47 0.5476684279713524 1104 48-49 0.4782659333681849 1104 50-51 0.43543378802880284 1104 52-53 0.43993856631553996 1104 54-55 0.5378151787508685 1104 56-57 0.6509746835786601 1104 58-59 0.5964702674548903 1104 60-61 0.6692813187120379 1104 62-63 0.5384893175435721 1104 64-65 0.5134217686568547 1104 66-67 0.5470531904097555 1104 68-69 0.7406197807289416 1104 70-71 0.6897659831030438 1104 72-73 0.7288688347121663 1104 74-75 0.6593664637146013 1104 76-77 0.6647753681318633 1104 78-79 0.7191867634846325 1104 80-81 0.9205124214258298 1104 82-83 0.9182074002800888 1104 84-85 0.8741185143380328 1104 86-87 0.8997758616887666 1104 88-89 0.9573459962251967 1104 90-91 1.1540540948464049 1104 92-93 1.3097215686617396 1104 94-95 1.2392650194265755 1104 96-97 1.2562460714474284 1104 98-99 1.5337739145392142 1104 100-101 1.546139479121571 1105 1 0.2724430993337421 1105 2 0.3190209955890744 1105 3 0.3348255642635394 1105 4 0.3275167817937472 1105 5 0.3640402917543568 1105 6 0.3318647149250751 1105 7 0.3659393053254263 1105 8 0.3972960351526922 1105 9 0.3503521636884699 1105 10-11 0.4828457022546999 1105 12-13 0.5018219143379525 1105 14-15 0.48766202655958324 1105 16-17 0.5807702769246816 1105 18-19 0.5647989852366706 1105 20-21 0.6426832275798731 1105 22-23 0.6524851164052592 1105 24-25 0.6341291695668403 1105 26-27 0.645536915840367 1105 28-29 0.6013777768922921 1105 30-31 0.5673209509490462 1105 32-33 0.7123731415516232 1105 34-35 0.6995366602279205 1105 36-37 0.713742356200477 1105 38-39 0.8061035965185326 1105 40-41 0.9846413736751245 1105 42-43 0.9018313260834034 1105 44-45 0.8252930286215729 1105 46-47 1.025609508925804 1105 48-49 0.9468083394121223 1105 50-51 0.9709411424949437 1105 52-53 0.7766385998660823 1105 54-55 0.9161177454790348 1105 56-57 0.9925287946842474 1105 58-59 1.0463289206941724 1105 60-61 1.0225300993134638 1105 62-63 1.1601576348608944 1105 64-65 1.1450707216247409 1105 66-67 1.1718047977224302 1105 68-69 1.274896112638281 1105 70-71 1.2482464527926567 1105 72-73 1.1720557685468336 1105 74-75 1.2391662176175977 1105 76-77 1.233156113437591 1105 78-79 1.3047028751340193 1105 80-81 1.2482248525497965 1105 82-83 1.3522756203053277 1105 84-85 1.4626762129771542 1105 86-87 1.4231444611620816 1105 88-89 1.2945737004590576 1105 90-91 1.4459868701388707 1105 92-93 1.6329703447837858 1105 94-95 1.7141304701213897 1105 96-97 1.7557925792574487 1105 98-99 1.992936817408836 1105 100-101 2.100152038400534 1106 1 0.16208416969614703 1106 2 0.2167284000357661 1106 3 0.22586906643871885 1106 4 0.1602440856107492 1106 5 0.19529762132013673 1106 6 0.21221314271095082 1106 7 0.24205279372137767 1106 8 0.1167603694678121 1106 9 0.19377938671103578 1106 10-11 -0.0052785131344279534 1106 12-13 -0.09348474115713401 1106 14-15 0.05732321715893107 1106 16-17 0.10139708215309184 1106 18-19 0.06093068444197769 1106 20-21 0.2038229563556584 1106 22-23 0.11035445171071956 1106 24-25 0.12577243149612372 1106 26-27 0.12252232866858748 1106 28-29 0.3866756119718957 1106 30-31 0.4861795407370266 1106 32-33 0.5063184036775539 1106 34-35 0.2911616828489372 1106 36-37 0.43078584982598045 1106 38-39 0.4461807715916706 1106 40-41 0.24087373106296894 1106 42-43 0.3965265372675262 1106 44-45 0.2878175198802353 1106 46-47 0.5555547482525967 1106 48-49 0.7275856759316284 1106 50-51 0.6005235905806003 1106 52-53 0.5989905871669592 1106 54-55 0.5876564448738648 1106 56-57 0.5709891690745508 1106 58-59 0.5042130754376117 1106 60-61 0.47098681159265254 1106 62-63 0.7705238317228762 1106 64-65 0.8120199550750584 1106 66-67 0.8114762906810569 1106 68-69 0.7068049274964778 1106 70-71 0.669418727334147 1106 72-73 0.6828148230096929 1106 74-75 0.7605448243339374 1106 76-77 0.9468145054430721 1106 78-79 0.8904306164094287 1106 80-81 0.731299059460504 1106 82-83 0.5907106876553989 1106 84-85 0.6003718758691399 1106 86-87 0.5988180687405169 1106 88-89 0.7760992761016006 1106 90-91 0.8977832282619893 1106 92-93 1.0287492273696657 1106 94-95 1.0514378352395681 1106 96-97 0.9747167165595378 1106 98-99 1.1141288986991533 1106 100-101 0.9857374275746515 1107 1 0.4166038936963332 1107 2 0.4542147816236728 1107 3 0.5002387609323407 1107 4 0.4404678815161418 1107 5 0.3342367470180818 1107 6 0.28898194746831507 1107 7 0.31674808175079505 1107 8 0.22920453397823337 1107 9 0.5096138549120468 1107 10-11 0.505876611927988 1107 12-13 0.5409653587112189 1107 14-15 0.6062198420624654 1107 16-17 0.7009034714580977 1107 18-19 0.7393929954865115 1107 20-21 0.7662260953996451 1107 22-23 0.7682437113230449 1107 24-25 0.8273258383688855 1107 26-27 0.7505916350374662 1107 28-29 0.7764906852843438 1107 30-31 0.7612759477459718 1107 32-33 0.8764765474879823 1107 34-35 1.0483064125234591 1107 36-37 0.933332095683717 1107 38-39 0.9002683947248116 1107 40-41 0.9757181407048066 1107 42-43 0.9982430813684786 1107 44-45 1.022304559691392 1107 46-47 1.107497187764153 1107 48-49 1.1541666033454945 1107 50-51 1.1428392313268887 1107 52-53 1.1271043803486762 1107 54-55 1.2128973205377562 1107 56-57 1.2123945324591787 1107 58-59 1.3084410817020782 1107 60-61 1.4610705648270255 1107 62-63 1.424447352990299 1107 64-65 1.4159259426369104 1107 66-67 1.5713189980213826 1107 68-69 1.7218753460356808 1107 70-71 1.5615552292180297 1107 72-73 1.4894412586173047 1107 74-75 1.5293885644038419 1107 76-77 1.4995102133735223 1107 78-79 1.5740038040257502 1107 80-81 1.637951256564321 1107 82-83 1.8181604697608051 1107 84-85 1.9008776863916346 1107 86-87 1.8082715164685084 1107 88-89 1.8321084004377042 1107 90-91 1.9998743354388928 1107 92-93 1.9625803704083893 1107 94-95 2.0763101626261964 1107 96-97 2.146272506654416 1107 98-99 2.4291198197577586 1107 100-101 2.287819399066777 1108 1 0.26056116830577736 1108 2 0.2715990278122611 1108 3 0.1622037524625526 1108 4 0.2647950036693558 1108 5 0.3172162313019129 1108 6 0.24271241989443837 1108 7 0.19631471077394735 1108 8 0.29891044401022526 1108 9 0.23811987787458833 1108 10-11 0.34157842822762774 1108 12-13 0.3280496331324514 1108 14-15 0.39293285730808947 1108 16-17 0.41042798679782777 1108 18-19 0.28751340768301503 1108 20-21 0.42713542158071505 1108 22-23 0.41779245967614287 1108 24-25 0.4472317295791228 1108 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>>Per sequence quality scores pass #Quality Count 2 12909.0 3 2398.0 4 2349.0 5 2569.0 6 2998.0 7 3618.0 8 4432.0 9 5558.0 10 6982.0 11 9075.0 12 12138.0 13 15509.0 14 18087.0 15 21979.0 16 27594.0 17 35881.0 18 30588.0 19 21818.0 20 21975.0 21 22886.0 22 25164.0 23 28008.0 24 31490.0 25 35798.0 26 41602.0 27 48585.0 28 58404.0 29 69971.0 30 85515.0 31 107008.0 32 135106.0 33 177095.0 34 237756.0 35 331046.0 36 477230.0 37 650828.0 38 506171.0 39 57103.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08888906010432 19.197135276062166 15.12600435137437 22.587971312459143 2 14.74432741304324 20.141782461403483 41.650056856941184 23.463833268612095 3 18.788485882808025 24.841110135008254 30.306009232761433 26.064394749422288 4 12.289777663507413 16.60629459222413 38.32217271932628 32.78175502494217 5 13.221688038935147 37.67203713413135 34.716117623012735 14.390157203920767 6 31.02269413901252 36.527484433208954 18.12831446471996 14.321506963058567 7 27.79432362130797 33.127176266807794 21.280944279282 17.79755583260224 8 25.652585850435738 35.22378579265898 20.304683617476403 18.81894473942888 9 25.97993839723276 17.144100447145508 21.14594172159161 35.730019434030126 10-11 24.239994150556274 27.023305119240142 27.96190993810424 20.774790792099342 12-13 25.126697658337477 24.387343687998957 28.648582485537126 21.837376168126436 14-15 22.228538875266395 25.766492791787805 27.16787705152055 24.83709128142525 16-17 21.229416772735835 29.126201667124686 27.767968753584043 21.876412806555436 18-19 21.134227581045483 27.627160358257512 30.350320606407628 20.88829145428937 20-21 22.07259412541848 26.536492984690856 30.487280460836914 20.90363242905375 22-23 21.699586422535695 26.48013108508366 30.34986113943507 21.470421352945582 24-25 21.454699420063665 26.568918922718716 30.536170656112272 21.440211001105354 26-27 21.2441339914017 26.876686176864506 30.464367373818845 21.41481245791495 28-29 21.073252516892506 27.05089257144109 30.567171451211905 21.308683460454496 30-31 21.319569618495883 26.742599164118953 30.47869648850926 21.459134728875902 32-33 21.204857046481298 26.75872117333086 30.36266813413936 21.67375364604849 34-35 21.595047061323875 26.811590127511646 30.266508167577268 21.326854643587208 36-37 21.520524103929937 26.666144784435758 30.26473487371461 21.548596237919696 38-39 21.296334383183304 26.981593051399837 30.23838686700128 21.483685698415577 40-41 21.599260082135498 26.83852011692383 30.117000769983314 21.445219030957354 42-43 21.301092417214853 26.797169934871683 29.997056639925844 21.90468100798762 44-45 21.495889861184033 26.958301935823 29.805935268939283 21.739872934053686 46-47 21.593069490518882 27.193867894303246 29.407798732161073 21.8052638830168 48-49 21.661157851674638 27.115358315705716 29.37611856177599 21.847365270843657 50-51 21.689997472150736 27.38969075582693 29.204606605678602 21.715705166343728 52-53 21.711117160509232 27.54882582218648 28.87312591397845 21.866931103325843 54-55 21.587108408648874 27.420469735739427 29.02943251302006 21.962989342591648 56-57 21.70286392526567 27.677612473831726 28.888961881650815 21.73056171925179 58-59 21.834920674377283 27.328381326429994 28.95694470584475 21.879753293347964 60-61 21.77162322603553 27.35495563470664 29.254492933731306 21.618928205526522 62-63 21.6007640369975 27.412061398155156 29.26732195711243 21.719852607734914 64-65 21.532261166757905 27.353667016822264 29.16647679356108 21.94759502285875 66-67 21.60079727982705 27.38851797504417 28.94512812728307 22.065556617845704 68-69 21.447500277047492 27.39105301571928 29.11942988434728 22.042016822885945 70-71 21.637763829292677 27.31958419685267 28.981817304831313 22.06083466902334 72-73 21.700471264020887 27.344631715127615 28.78962733863957 22.165269682211928 74-75 21.453790014488504 27.635650867719548 28.734223163229416 22.176335954562532 76-77 21.54274818185006 27.409937379973105 28.70606140731042 22.341253030866415 78-79 21.600212960429637 27.376705425800264 28.747053704589614 22.276027909180485 80-81 21.53980206387648 27.7126848623017 28.584662613722717 22.162850460099097 82-83 21.698914706618112 27.59939400027131 28.633916191142884 22.067775101967694 84-85 21.64714535595178 27.433240549413355 28.525729785768554 22.39388430886631 86-87 21.487969482222397 27.39133567203153 28.782142614630658 22.338552231115415 88-89 21.665295938873086 27.268813405407133 28.70422357258266 22.361667083137117 90-91 21.66262157469187 27.218837996285732 28.768371582681446 22.350168846340953 92-93 21.679710125844217 27.053836244655514 28.836300136512843 22.430153492987422 94-95 21.449064460137546 27.158869860034457 28.83532285105751 22.556742828770492 96-97 21.362914876151912 27.18650924932429 28.828081584147636 22.622494290376167 98-99 21.355382407903242 27.183112742461418 28.794977077262562 22.66652777237277 100-101 21.589237825260977 26.765762682927512 29.102852764552793 22.542146727258714 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3971.0 1 2333.5 2 667.5 3 631.0 4 545.5 5 536.5 6 840.5 7 1776.0 8 3065.0 9 3687.5 10 3593.0 11 3488.5 12 3643.5 13 3962.5 14 4305.0 15 4642.5 16 5003.0 17 5313.0 18 5674.5 19 6172.0 20 6722.0 21 7647.0 22 9209.5 23 11084.5 24 13439.0 25 17004.5 26 21840.5 27 27437.5 28 32503.5 29 37940.0 30 44827.5 31 52169.0 32 59630.5 33 67316.0 34 76132.0 35 84719.5 36 94067.0 37 101893.5 38 105123.0 39 106878.5 40 108940.0 41 113876.5 42 118261.0 43 121653.0 44 127958.0 45 132794.0 46 133440.0 47 133846.5 48 136117.0 49 132654.0 50 124850.5 51 117450.5 52 109062.0 53 101589.0 54 97065.0 55 89846.0 56 78233.0 57 68588.0 58 61187.0 59 53161.0 60 43693.0 61 35447.0 62 28061.0 63 20987.0 64 15566.0 65 11638.0 66 8161.0 67 5908.0 68 4435.0 69 3066.0 70 2237.5 71 1602.0 72 1211.5 73 975.5 74 699.5 75 489.5 76 338.0 77 203.5 78 124.5 79 88.5 80 67.5 81 43.0 82 30.5 83 27.0 84 21.5 85 19.5 86 14.0 87 9.5 88 7.0 89 7.0 90 7.0 91 5.0 92 5.0 93 3.0 94 1.5 95 2.0 96 0.5 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3149860762743082 2 0.27212339253858453 3 0.2655950306463758 4 0.2723597133310627 5 0.27646578710037045 6 0.20808045777700712 7 0.1990116473656583 8 0.19815498449292498 9 0.21073906669238623 10-11 0.2106209062961472 12-13 0.20706132435944513 14-15 0.20777028673687956 16-17 0.19311839760323452 18-19 0.19819929464151465 20-21 0.18707744734551193 22-23 0.1967370597380561 24-25 0.19698815058006416 26-27 0.1965598191436975 28-29 0.20548092905974752 30-31 0.2031620312835557 32-33 0.2093063718879875 34-35 0.2029700206396672 36-37 0.19004622730101864 38-39 0.17771323594356542 40-41 0.17532048791972418 42-43 0.1901643876972577 44-45 0.19233558497815068 46-47 0.19164139265024613 48-49 0.20698747411179566 50-51 0.20273369984718906 52-53 0.22103379121471545 54-55 0.19199587383896333 56-57 0.17424227430404265 58-59 0.17703381366519075 60-61 0.16591196636918804 62-63 0.1716722856858428 64-65 0.17071223246640035 66-67 0.16950108840494985 68-69 0.17264710895481516 70-71 0.17397641341250475 72-73 0.17153935524007385 74-75 0.1873876183856395 76-77 0.17837788817241018 78-79 0.18307476392291333 80-81 0.1759703700990391 82-83 0.1583201609108276 84-85 0.15753734828574373 86-87 0.1603584277459516 88-89 0.16653230844944314 90-91 0.1720710770231497 92-93 0.1681865539967902 94-95 0.1678763829566626 96-97 0.17272095920246458 98-99 0.18198178025770193 100-101 0.16475990250585706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3385229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.1580241939501 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21653029172951 35.00863919090019 2 14.775987201984888 14.527166727293404 3 5.573606992080863 8.219625220928412 4 2.780996152311276 5.468331045543993 5 1.5463289428032017 3.800723779106253 6 0.9735582079764324 2.8714918765154502 7 0.6324686749580979 2.1763637297854013 8 0.4419468301148235 1.7380186373779236 9 0.3157120736968753 1.3967803581401836 >10 1.5682601073831175 13.735430149888636 >50 0.11367186532165625 3.8651450931611415 >100 0.058168317222652324 4.981212510386935 >500 0.0018629264126441324 0.6206314883551673 >1k 7.211328031202263E-4 0.6152845263907419 >5k 6.0094400260018866E-5 0.21715750688968424 >10k+ 1.2018880052003773E-4 0.7579981593364723 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14950 0.4416244809435344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10709 0.3163449208310575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7351 0.21714926818835592 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4463 0.13183746210374542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9540099059768186E-5 0.0 4 0.0 0.0 0.0 2.9540099059768186E-5 0.0 5 0.0 0.0 0.0 2.9540099059768186E-5 0.0 6 0.0 0.0 0.0 2.9540099059768186E-5 0.0 7 0.0 0.0 0.0 2.9540099059768186E-5 0.0 8 0.0 0.0 0.0 2.9540099059768186E-5 0.0 9 0.0 0.0 0.0 2.9540099059768186E-5 0.0 10-11 0.0 0.0 0.0 7.385024764942047E-5 0.0 12-13 0.0 0.0 0.0 1.0339034670918865E-4 0.0 14-15 0.0 0.0 0.0 1.1816039623907274E-4 0.0 16-17 0.0 0.0 0.0 1.1816039623907274E-4 0.0 18-19 0.0 0.0 0.0 1.4770049529884093E-4 0.0 20-21 0.0 0.0 0.0 1.9201064388849322E-4 0.0 22-23 0.0 0.0 0.0 3.1017104012756597E-4 0.0 24-25 0.0 0.0 0.0 5.612618821355955E-4 0.0 26-27 0.0 0.0 0.0 6.794222783746683E-4 0.0 28-29 0.0 0.0 0.0 0.0012997643586298002 0.0 30-31 0.0 0.0 0.0 0.0024813683210205277 0.0 32-33 0.0 0.0 0.0 0.004844576245801983 0.0 34-35 0.0 0.0 0.0 0.007488415111651235 0.0 36-37 0.0 0.0 0.0 0.011284317840831448 0.0 38-39 0.0 0.0 0.0 0.014755279480354211 0.0 40-41 0.0 0.0 0.0 0.0194078450822677 0.0 42-43 0.0 0.0 0.0 0.027368901778875226 0.0 44-45 0.0 0.0 0.0 0.038667989669236555 0.0 46-47 0.0 0.0 0.0 0.06306811149260508 0.0 48-49 0.0 0.0 0.0 0.10145547022077384 0.0 50-51 0.0 0.0 0.0 0.1334326274529729 0.0 52-53 0.0 0.0 0.0 0.1653802445861122 0.0 54-55 0.0 0.0 0.0 0.20325065158073502 0.0 56-57 0.0 0.0 0.0 0.24089950783240957 0.0 58-59 0.0 0.0 0.0 0.28845906731863635 0.0 60-61 0.0 0.0 0.0 0.35743519862319506 0.0 62-63 0.0 0.0 0.0 0.4104153662868893 0.0 64-65 0.0 0.0 0.0 0.47566944510991727 0.0 66-67 0.0 0.0 0.0 0.5795176633545323 0.0 68-69 0.0 0.0 0.0 0.6909724571070377 0.0 70-71 0.0 0.0 0.0 0.7917041949008472 0.0 72-73 2.9540099059768186E-5 0.0 0.0 0.907944484701035 0.0 74-75 2.9540099059768186E-5 0.0 0.0 1.0224271386071666 0.0 76-77 2.9540099059768186E-5 0.0 0.0 1.1300417194819021 0.0 78-79 2.9540099059768186E-5 0.0 0.0 1.2610077486633844 0.0 80-81 2.9540099059768186E-5 0.0 0.0 1.4272446561222298 0.0 82-83 2.9540099059768186E-5 0.0 0.0 1.5949733385835936 0.0 84-85 2.9540099059768186E-5 0.0 0.0 1.791813788668359 0.0 86-87 2.9540099059768186E-5 0.0 0.0 2.07071663394116 0.0 88-89 2.9540099059768186E-5 0.0 0.0 2.4460826726936347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10335 0.0 36.992382 1 GTATCAA 16360 0.0 35.983555 1 TATCAAC 19915 0.0 29.358717 2 ATCAACG 19770 0.0 29.33317 3 TCAACGC 19980 0.0 29.050806 4 CAACGCA 20200 0.0 28.685219 5 AACGCAG 20720 0.0 28.065056 6 GTACATG 14710 0.0 25.343704 1 ACGCAGA 23345 0.0 24.665472 7 TACATGG 14790 0.0 24.554857 2 CGCAGAG 23385 0.0 24.399803 8 ACATGGG 14995 0.0 23.077604 3 GAGTACT 12445 0.0 22.44276 12-13 GCAGAGT 24980 0.0 22.385391 9 GGACCGA 815 0.0 22.15135 6 AGAGTAC 20615 0.0 20.77221 10-11 AGTACTT 13250 0.0 20.523596 12-13 GTACTTT 13505 0.0 20.451864 14-15 CATGGGG 7295 0.0 20.135906 4 AGGACCG 1045 0.0 19.55951 5 >>END_MODULE