FastQCFastQC Report
Thu 26 May 2016
SRR937830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385229
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165250.4881501369626693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128130.3784972892528098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.26302504202817595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA175600.034.9884341
GGTATCA116400.034.2907371
ATCAACG217300.028.1148343
TCAACGC218800.027.9003774
TATCAAC219400.027.8673822
CAACGCA221400.027.572735
AACGCAG227250.026.9618856
GTACATG145150.024.5851821
ACGCAGA256000.023.7323047
TACATGG147650.023.4556962
CGCAGAG256250.023.4125568
GGACCGA8550.023.3317666
ACATGGG149800.022.1993623
GAGTACT145750.022.04796812-13
GCAGAGT273300.021.5348079
AGTACTT150450.021.09082812-13
GTACTTT154250.020.58451814-15
AGAGTAC227000.020.48642510-11
AGGACCG11450.020.330355
GTGTAGG15850.019.1867391
Produced by FastQC (version 0.11.5)