##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3385229 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23584519688328 33.0 31.0 34.0 31.0 34.0 2 32.449398253412106 34.0 31.0 34.0 31.0 34.0 3 32.543178024293184 34.0 31.0 34.0 31.0 34.0 4 36.15881938858494 37.0 35.0 37.0 35.0 37.0 5 36.07575676564274 37.0 35.0 37.0 35.0 37.0 6 36.1070438661609 37.0 35.0 37.0 35.0 37.0 7 36.0531497278323 37.0 35.0 37.0 35.0 37.0 8 36.03420359449834 37.0 35.0 37.0 35.0 37.0 9 37.70883712741443 39.0 37.0 39.0 35.0 39.0 10-11 37.64739372137011 39.0 37.0 39.0 35.0 39.0 12-13 37.55570287859403 39.0 37.0 39.0 35.0 39.0 14-15 38.78461398032452 40.0 38.0 41.0 35.0 41.0 16-17 38.75339009561834 40.0 38.0 41.0 35.0 41.0 18-19 38.74079183417133 40.0 38.0 41.0 35.0 41.0 20-21 38.62906733931442 40.0 38.0 41.0 34.0 41.0 22-23 38.477518803011556 40.0 38.0 41.0 34.0 41.0 24-25 38.55870415265851 40.0 38.0 41.0 34.0 41.0 26-27 38.440871060717015 40.0 38.0 41.0 34.0 41.0 28-29 38.301984887876124 40.0 38.0 41.0 34.0 41.0 30-31 38.3487824605071 40.0 38.0 41.0 34.0 41.0 32-33 38.28399289974179 40.0 38.0 41.0 34.0 41.0 34-35 38.16272916839599 40.0 38.0 41.0 34.0 41.0 36-37 38.00961456374148 40.0 37.5 41.0 33.5 41.0 38-39 37.87784814557597 40.0 37.0 41.0 33.0 41.0 40-41 37.70639903533852 40.0 37.0 41.0 33.0 41.0 42-43 37.489143275093056 40.0 37.0 41.0 33.0 41.0 44-45 37.18610336257902 40.0 37.0 41.0 32.0 41.0 46-47 36.838648434123655 40.0 36.0 41.0 31.0 41.0 48-49 36.52419703364234 39.5 36.0 41.0 30.5 41.0 50-51 36.2759652301218 39.0 36.0 41.0 30.0 41.0 52-53 36.04715870034198 39.0 35.5 40.5 29.5 41.0 54-55 35.827672810318 39.0 35.0 40.0 29.0 41.0 56-57 35.57435730344978 39.0 35.0 40.0 28.0 41.0 58-59 35.316631312091445 38.0 35.0 40.0 28.0 41.0 60-61 34.97535351375047 38.0 34.0 40.0 27.0 41.0 62-63 34.6360584468584 38.0 34.0 40.0 26.0 41.0 64-65 34.25851766601314 37.0 34.0 40.0 26.0 41.0 66-67 33.83277704403454 36.5 33.0 39.0 25.5 41.0 68-69 33.39369389781312 36.0 33.0 39.0 25.0 40.0 70-71 32.981820727637626 35.5 32.0 38.5 23.5 40.0 72-73 32.492973444337146 35.0 32.0 38.0 22.5 40.0 74-75 31.98692569985664 35.0 31.0 37.0 21.5 39.0 76-77 30.73497036684963 34.0 29.5 36.0 20.5 38.5 78-79 31.336787112481904 34.0 31.0 36.0 20.5 39.0 80-81 31.136736835233304 34.0 31.0 36.0 20.0 37.5 82-83 30.77120927417318 34.0 31.0 35.5 18.5 37.0 84-85 30.37588520599345 34.0 30.5 35.0 17.5 37.0 86-87 29.955360774706822 34.0 30.0 35.0 12.5 36.0 88-89 29.594162315163906 34.0 30.0 35.0 7.0 36.0 90-91 29.07672435158744 34.0 29.0 35.0 2.0 35.5 92-93 28.5912406516664 34.0 29.0 35.0 2.0 35.0 94-95 28.123738896246014 33.0 29.0 35.0 2.0 35.0 96-97 27.57638345884429 33.0 27.0 35.0 2.0 35.0 98-99 26.71247129810125 33.0 25.5 34.5 2.0 35.0 100-101 24.05038831937219 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30063943153010797 1101 2 0.28719422329884736 1101 3 0.20393028419397297 1101 4 0.18833063197426014 1101 5 0.13391843796485148 1101 6 0.1485657135691909 1101 7 0.1816727882852902 1101 8 0.1586638898606907 1101 9 0.2968941070026716 1101 10-11 0.29355627452199684 1101 12-13 0.27233548450521283 1101 14-15 0.2858797654732186 1101 16-17 0.29447709602458616 1101 18-19 0.296161379657228 1101 20-21 0.32740822184818796 1101 22-23 0.38805232398403433 1101 24-25 0.2917998532188406 1101 26-27 0.25471535453575456 1101 28-29 0.29298351949896784 1101 30-31 0.36303684291576843 1101 32-33 0.3846373308947335 1101 34-35 0.37139620465013223 1101 36-37 0.3333634668839025 1101 38-39 0.3303431776958021 1101 40-41 0.31871321283836096 1101 42-43 0.4930265335809523 1101 44-45 0.43660691599750123 1101 46-47 0.5072309805362494 1101 48-49 0.46873750747286635 1101 50-51 0.5191556517379254 1101 52-53 0.34900464642834805 1101 54-55 0.42011238733444856 1101 56-57 0.40465499616832545 1101 58-59 0.37159340222925863 1101 60-61 0.35646197185607065 1101 62-63 0.4268545256485581 1101 64-65 0.49403420721286295 1101 66-67 0.4417573948744007 1101 68-69 0.51939569652437 1101 70-71 0.5018144418945312 1101 72-73 0.35426140667400574 1101 74-75 0.3699670109136193 1101 76-77 0.3783847010693542 1101 78-79 0.41899593557682735 1101 80-81 0.38649295001340533 1101 82-83 0.40879163315167233 1101 84-85 0.4474844181677291 1101 86-87 0.4163700583910739 1101 88-89 0.4420245535843996 1101 90-91 0.38567660419463934 1101 92-93 0.49840098650524567 1101 94-95 0.49661300275374387 1101 96-97 0.6681319617580961 1101 98-99 0.6599488541777951 1101 100-101 1.1018440663341238 1102 1 0.2458679440333924 1102 2 0.236332156078781 1102 3 0.24745361368437813 1102 4 0.10892868718526927 1102 5 0.1395256307603887 1102 6 0.1565756881750886 1102 7 0.18560488548343557 1102 8 0.10849630336139882 1102 9 0.211558988671662 1102 10-11 0.18000361486330974 1102 12-13 0.22769665294979546 1102 14-15 0.2604865537286756 1102 16-17 0.2909086799549101 1102 18-19 0.2761408570432735 1102 20-21 0.33892518236321934 1102 22-23 0.4013119654214137 1102 24-25 0.2258185155153427 1102 26-27 0.27168093137386506 1102 28-29 0.24213388409120995 1102 30-31 0.23581988025453882 1102 32-33 0.20715685025738395 1102 34-35 0.16307952468103082 1102 36-37 0.13842702098111204 1102 38-39 0.18350108074467641 1102 40-41 0.1661883603497074 1102 42-43 0.20422071241520712 1102 44-45 0.21495215673934354 1102 46-47 0.15461367909800572 1102 48-49 0.12010559399828225 1102 50-51 0.26161883514846096 1102 52-53 0.28806199919742426 1102 54-55 0.346413566817823 1102 56-57 0.18807606084169493 1102 58-59 0.19311444863960503 1102 60-61 0.22218560254458453 1102 62-63 0.2265399238573238 1102 64-65 0.28959224373161163 1102 66-67 0.3168664364464675 1102 68-69 0.22578329937534392 1102 70-71 0.15703707864548022 1102 72-73 0.2406348040534496 1102 74-75 0.22371528331689916 1102 76-77 0.12222048614234993 1102 78-79 0.1742381311725616 1102 80-81 0.11773381091033741 1102 82-83 0.123755445239361 1102 84-85 0.0729440813911495 1102 86-87 0.22388296297670962 1102 88-89 0.41325867619608303 1102 90-91 0.31441603867271084 1102 92-93 0.2462874771747039 1102 94-95 0.3912727092252908 1102 96-97 0.5063778856826922 1102 98-99 0.5675474451310798 1102 100-101 0.8811672898099197 1103 1 0.26988348915743643 1103 2 0.29056458075596936 1103 3 0.25517603014326085 1103 4 0.1305442100223999 1103 5 0.11740168764774239 1103 6 0.1410946390185046 1103 7 0.1773837992748568 1103 8 0.16414255428421853 1103 9 0.20258293317723997 1103 10-11 0.20593653124559097 1103 12-13 0.1971433802554472 1103 14-15 0.24098271620029976 1103 16-17 0.30382951961447446 1103 18-19 0.16181385226551726 1103 20-21 0.2383930822392486 1103 22-23 0.27124273668800924 1103 24-25 0.24039206930341805 1103 26-27 0.1857996927785024 1103 28-29 0.043992055728331536 1103 30-31 0.1421709549017791 1103 32-33 0.14866971398681983 1103 34-35 0.21563137658446863 1103 36-37 0.1792594373007077 1103 38-39 0.14904759771755494 1103 40-41 0.07855481296911648 1103 42-43 0.18427183302920724 1103 44-45 0.186724384455907 1103 46-47 0.18856222418025936 1103 48-49 0.05991559803779012 1103 50-51 0.07098491167616316 1103 52-53 -0.00912218823553701 1103 54-55 0.06413375458510018 1103 56-57 -0.08108163301965732 1103 58-59 -0.09691703162951626 1103 60-61 -0.05502741858619942 1103 62-63 0.02525912264268726 1103 64-65 0.17382216113072957 1103 66-67 0.19956552128996918 1103 68-69 0.18562924106719692 1103 70-71 -0.026179480533251365 1103 72-73 -0.003433393910079019 1103 74-75 0.021958099268765352 1103 76-77 0.10425305289863118 1103 78-79 0.15870763292069157 1103 80-81 0.10089186693407015 1103 82-83 0.15851398043179543 1103 84-85 0.12084005720984692 1103 86-87 0.17974163606943705 1103 88-89 0.2123247151146117 1103 90-91 0.2118913489483738 1103 92-93 0.2939501368395625 1103 94-95 0.15831593191337134 1103 96-97 0.45574479796975353 1103 98-99 0.5514848467552405 1103 100-101 0.9053263069569191 1104 1 0.23290877014332523 1104 2 0.23436757393406538 1104 3 0.0648927090152327 1104 4 0.07969779955477918 1104 5 0.15189586854904036 1104 6 0.12112231959214625 1104 7 0.16702454910452502 1104 8 0.15180344312602045 1104 9 0.20867252246454626 1104 10-11 0.15411867366448462 1104 12-13 0.20661641223323812 1104 14-15 0.2551323429301391 1104 16-17 0.28407232619942135 1104 18-19 0.18845606211109356 1104 20-21 0.21616407897435863 1104 22-23 0.1812809579227732 1104 24-25 0.12373819267963881 1104 26-27 0.1324382590021429 1104 28-29 0.13287965914754807 1104 30-31 0.2657404396621459 1104 32-33 0.24314635792520534 1104 34-35 0.2776168048349845 1104 36-37 0.3735047052558187 1104 38-39 0.41341175146026643 1104 40-41 0.4675971120141327 1104 42-43 0.4347892821104864 1104 44-45 0.45508227472863894 1104 46-47 0.45641883832959707 1104 48-49 0.46874093300199604 1104 50-51 0.5706459641301151 1104 52-53 0.5799299258267681 1104 54-55 0.4577410635298378 1104 56-57 0.5704440930220755 1104 58-59 0.5497690009419642 1104 60-61 0.5562369695702714 1104 62-63 0.6293239346162167 1104 64-65 0.6048661024583168 1104 66-67 0.5294137248166351 1104 68-69 0.5387746760059571 1104 70-71 0.5789272892273303 1104 72-73 0.5607721364884881 1104 74-75 0.38637356150644564 1104 76-77 0.42800674679365613 1104 78-79 0.503239085216368 1104 80-81 0.5967588632666612 1104 82-83 0.6908779089510872 1104 84-85 0.7144274023922783 1104 86-87 0.699411760782624 1104 88-89 0.6553882447180577 1104 90-91 0.6372515652176141 1104 92-93 0.679154866805078 1104 94-95 0.7046056756738182 1104 96-97 1.0241100302519328 1104 98-99 0.9794651677739061 1104 100-101 1.1760689811632083 1105 1 0.221365324435844 1105 2 0.21607236234394378 1105 3 0.1854797045757266 1105 4 0.0827498682881398 1105 5 0.043155751735831416 1105 6 0.07607008333006604 1105 7 0.1160125905101026 1105 8 0.09818690562220667 1105 9 0.21607460438967507 1105 10-11 0.16271898910225246 1105 12-13 0.17494291227601622 1105 14-15 0.17468406473570752 1105 16-17 0.1553537820688149 1105 18-19 0.23995416755833787 1105 20-21 0.30044594291506144 1105 22-23 0.32984166670365767 1105 24-25 0.2634141903378264 1105 26-27 0.15951797803765544 1105 28-29 0.12307076283483553 1105 30-31 0.15776123472479497 1105 32-33 0.12880589924589003 1105 34-35 0.17275820310853618 1105 36-37 0.18222694935233363 1105 38-39 0.1674781080644081 1105 40-41 0.18308760610291586 1105 42-43 0.1483346064689428 1105 44-45 0.1108637402286945 1105 46-47 -0.08580296880363392 1105 48-49 -0.07426790077860801 1105 50-51 0.06354444844311047 1105 52-53 0.030143480154677604 1105 54-55 0.046882156309841605 1105 56-57 0.008345719618468195 1105 58-59 0.13020464729152081 1105 60-61 0.22223835907320222 1105 62-63 0.2011543830064042 1105 64-65 0.12535688098758158 1105 66-67 0.1569137886477705 1105 68-69 0.07599400380367882 1105 70-71 0.13195864083920839 1105 72-73 0.22099629350321237 1105 74-75 0.1290614926745377 1105 76-77 0.04610289484245911 1105 78-79 0.28595522750469726 1105 80-81 0.23115627275299744 1105 82-83 0.27063112212063345 1105 84-85 0.46336984373371237 1105 86-87 0.36286317498932874 1105 88-89 0.4169675030495874 1105 90-91 0.41934534277978486 1105 92-93 0.40611252280051957 1105 94-95 0.4174935617793736 1105 96-97 0.5899598824100742 1105 98-99 0.7633211324907485 1105 100-101 0.9620750023425977 1106 1 0.15626581333480516 1106 2 0.12487924084307878 1106 3 0.16025873108114297 1106 4 0.0766892881356398 1106 5 0.09953573352501621 1106 6 0.08850986155147211 1106 7 0.04780983209971623 1106 8 0.09206362784751576 1106 9 0.12635630195629943 1106 10-11 0.01827777453531354 1106 12-13 -0.027332868914989206 1106 14-15 0.17723685542112122 1106 16-17 0.20383999072934955 1106 18-19 0.1876369916872349 1106 20-21 0.17746298954357087 1106 22-23 0.19106176243152362 1106 24-25 0.26831714439329346 1106 26-27 0.18437202794781626 1106 28-29 0.2775032709819669 1106 30-31 0.24441789827083227 1106 32-33 0.21685459249920314 1106 34-35 0.1011743329268171 1106 36-37 0.09326073339607177 1106 38-39 0.035755533643047954 1106 40-41 0.06995715657521373 1106 42-43 -0.056382107149694605 1106 44-45 -0.1014362507941513 1106 46-47 0.06779751339808371 1106 48-49 0.170451463239516 1106 50-51 0.25810101238541705 1106 52-53 0.11682123290090374 1106 54-55 0.0659941014893235 1106 56-57 -0.0415823918579008 1106 58-59 0.010490519949797772 1106 60-61 0.09177942672821615 1106 62-63 0.011559338398463126 1106 64-65 0.027255906467047453 1106 66-67 0.032127993649041287 1106 68-69 -0.06826154030481746 1106 70-71 -0.03642366076200432 1106 72-73 0.1475427517287855 1106 74-75 0.014340090983701259 1106 76-77 0.008519660186635747 1106 78-79 0.007161700749197308 1106 80-81 -0.02380332290433884 1106 82-83 0.027541611290331502 1106 84-85 0.07674196409604761 1106 86-87 0.0709741098821226 1106 88-89 -0.23596453700279696 1106 90-91 -0.03226306497135312 1106 92-93 0.06309440176276127 1106 94-95 0.2837725006506133 1106 96-97 0.37865906349363243 1106 98-99 0.36460743120786887 1106 100-101 0.7414833932793741 1107 1 0.3413055337034123 1107 2 0.30289651352908464 1107 3 0.2635445134589176 1107 4 0.16084403869172803 1107 5 0.17922024031152972 1107 6 0.16294174679365625 1107 7 0.23729221895340658 1107 8 0.18981727077275679 1107 9 0.3193823131233984 1107 10-11 0.2751255660828278 1107 12-13 0.33331281404837654 1107 14-15 0.41448173716791104 1107 16-17 0.3839882044476326 1107 18-19 0.41538711652267324 1107 20-21 0.42419701060671144 1107 22-23 0.4600130312135846 1107 24-25 0.42505042779244206 1107 26-27 0.4622886378711044 1107 28-29 0.4661777455382392 1107 30-31 0.3506853752330201 1107 32-33 0.3857920726390205 1107 34-35 0.5037925293951062 1107 36-37 0.35192158954024677 1107 38-39 0.4343713922839285 1107 40-41 0.4540242386034521 1107 42-43 0.46717802948837317 1107 44-45 0.5329932512259177 1107 46-47 0.4406400164622184 1107 48-49 0.4512080227746722 1107 50-51 0.4409675746442687 1107 52-53 0.3781509006116508 1107 54-55 0.4675390535979105 1107 56-57 0.43109742792511696 1107 58-59 0.4581736308490676 1107 60-61 0.4621750983429038 1107 62-63 0.5750373106169064 1107 64-65 0.578465732151372 1107 66-67 0.5271636284940229 1107 68-69 0.38949896856985333 1107 70-71 0.3353397533739084 1107 72-73 0.5325335025572357 1107 74-75 0.4325862097362503 1107 76-77 0.37035150684331697 1107 78-79 0.5603546509512043 1107 80-81 0.5922114190267536 1107 82-83 0.7187615940403447 1107 84-85 0.785807116849238 1107 86-87 0.8787843259609289 1107 88-89 0.7105370631606256 1107 90-91 0.9257480226858306 1107 92-93 1.0315390656149575 1107 94-95 1.1183530535581454 1107 96-97 1.3413294638749456 1107 98-99 1.4854904150019586 1107 100-101 1.6018008725112942 1108 1 0.21707628469155082 1108 2 0.22466905258422543 1108 3 0.13256342932566412 1108 4 0.09662137729515052 1108 5 0.0838146314276571 1108 6 0.06544127624970741 1108 7 0.12075212860897011 1108 8 0.05881689433759618 1108 9 0.18245590619698504 1108 10-11 0.13846548703143924 1108 12-13 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-0.13941112995231464 2316 70-71 -0.08009743874432473 2316 72-73 0.05967020206463047 2316 74-75 0.16263547081999974 2316 76-77 0.15631209797632195 2316 78-79 0.05832263124677084 2316 80-81 -0.09345360560378779 2316 82-83 -0.04269776556556337 2316 84-85 0.24982189517436026 2316 86-87 0.27723260182299825 2316 88-89 0.2982587380374717 2316 90-91 0.40322954977942516 2316 92-93 0.33660064196963546 2316 94-95 0.4304220190753867 2316 96-97 0.39443291215080833 2316 98-99 0.3919559322433379 2316 100-101 0.8744055523142507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 22.0 5 61.0 6 115.0 7 312.0 8 720.0 9 1391.0 10 2437.0 11 4151.0 12 6671.0 13 9692.0 14 12504.0 15 17446.0 16 23970.0 17 34144.0 18 30211.0 19 18515.0 20 15502.0 21 15628.0 22 16350.0 23 17836.0 24 20551.0 25 24588.0 26 29985.0 27 37201.0 28 47521.0 29 58859.0 30 74989.0 31 94816.0 32 122578.0 33 163037.0 34 225404.0 35 324028.0 36 501072.0 37 764082.0 38 615574.0 39 53252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21940347061008 20.17833183679487 15.460911685911885 22.141353006683158 2 15.246383627222857 21.114642465842046 40.334848838882095 23.304125068053004 3 19.058799271777477 25.16636245287985 30.27233903526172 25.50249924008095 4 12.857321364057848 17.391557365644744 37.53941763954693 32.21170363075047 5 13.287501761748906 38.18883643612896 34.27144554415659 14.252216257965541 6 31.225804812613863 37.240346221777024 17.3714097332854 14.162439232323724 7 27.95217103481035 33.61970490031842 20.725747061720195 17.702377003151042 8 25.992953504770284 35.72381661624664 19.734676738264977 18.548553140718102 9 26.190133665994235 17.238420207318324 20.704389570099984 35.86705655658746 10-11 24.522676168340805 27.550522446346303 27.31563427928636 20.61116710602654 12-13 25.407811650461632 24.82949965739671 28.184722478040232 21.57796621410143 14-15 22.372194022915437 26.21915681332046 26.805645940053097 24.60300322371101 16-17 21.352898134808605 29.64440514954823 27.349715484535906 21.652981231107262 18-19 21.24283172571191 28.033923258958254 30.063933636395056 20.659311378934778 20-21 22.158530486416133 26.900617358530248 30.357621301247274 20.583230853806345 22-23 21.83737153064617 26.87000702618417 30.15023213584921 21.14238930732045 24-25 21.630947512110335 26.990836807618745 30.358644918651724 21.019570761619192 26-27 21.44782955986213 27.23569454438895 30.345130526537183 20.971345369211736 28-29 21.256227877851174 27.45808054042589 30.296657609546546 20.989033972176387 30-31 21.496448246189548 27.15383508767058 30.264082577574513 21.085634088565353 32-33 21.4311350871684 27.193669911252684 30.116130991433664 21.259064010145252 34-35 21.797520935806705 27.195501397394384 30.073017216855934 20.933960449942973 36-37 21.74578145230352 27.075893536301383 30.00740865684419 21.170916354550904 38-39 21.501460686421442 27.46424275974688 29.94404948447274 21.090247069358938 40-41 21.8096046515372 27.310824888370817 29.792492534052972 21.08707792603901 42-43 21.56158019329559 27.324048940641195 29.647554999132126 21.466815866931093 44-45 21.782783643703816 27.497818832613515 29.445368311178992 21.274029212503677 46-47 21.895623604784195 27.720310797290225 28.999722027667847 21.384343570257727 48-49 21.945634992492383 27.749466875062218 28.882669385143515 21.42222874730188 50-51 22.055376460499424 27.953707119961457 28.675770531328897 21.31514588821022 52-53 22.099524138544247 28.150163548758446 28.302885860897447 21.447426451799863 54-55 21.917675288732312 28.13397557447369 28.3928650026335 21.555484134160498 56-57 22.014256642608228 28.277658616300407 28.37785863231114 21.330226108780234 58-59 22.07107702314969 28.003688967570582 28.357490734009428 21.5677432752703 60-61 22.048966259003453 28.061026299845594 28.708294180393708 21.18171326075725 62-63 21.828360799225106 28.08615015409593 28.73938513465411 21.34610391202486 64-65 21.835568583395688 28.01847378715 28.619038771084615 21.5269188583697 66-67 21.898533304541584 28.073359291203047 28.4074430415195 21.620664362735877 68-69 21.796236701758406 28.067996722223832 28.479269601816366 21.656496974201396 70-71 22.028320983354597 28.017458791924977 28.29057181812099 21.663648406599435 72-73 22.0377705614598 28.10108267417064 28.084554988746703 21.77659177562286 74-75 21.810607199690182 28.500612513954003 27.88839396094031 21.800386325415506 76-77 21.875772658216032 28.188462287189438 28.067466041440625 21.868299013153912 78-79 21.90287569910337 28.191371986946823 27.967236485330833 21.93851582861898 80-81 21.871633347857234 28.50227089779852 27.877157352908593 21.748938401435648 82-83 22.014754135891955 28.45712268501936 27.83162649245492 21.69649668663376 84-85 21.904016365294645 28.31169387446135 27.75171425722905 22.03257550301495 86-87 21.70922557971706 28.279165161352456 28.007174699259636 22.00443455967085 88-89 21.890424547349678 28.180397840146114 27.933649392699873 21.995528219804335 90-91 21.94283674946257 28.150675532250403 27.90571633982545 22.000771378461582 92-93 21.848174123323076 27.987829753620307 28.042834847942817 22.1211612751138 94-95 21.75242797459197 28.058574471623633 27.972199221972872 22.21679833181153 96-97 21.596042690169558 28.196364263687922 28.02079268492619 22.18680036121633 98-99 21.614052449282532 28.21449408080823 27.758963716794554 22.412489753114684 100-101 21.575274672352062 28.232057290450978 27.917069720361003 22.275598316835953 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 585.0 1 421.5 2 330.0 3 435.5 4 438.0 5 445.5 6 798.0 7 1715.5 8 2888.0 9 3619.0 10 3620.0 11 3590.0 12 3962.0 13 4404.0 14 4811.0 15 5166.0 16 5505.5 17 5896.0 18 6242.5 19 6685.0 20 7317.0 21 8247.0 22 9618.5 23 11492.5 24 14050.5 25 17672.0 26 22304.5 27 27618.0 28 32644.5 29 38444.0 30 45403.5 31 52170.5 32 59561.5 33 67328.5 34 76417.5 35 84672.5 36 93712.0 37 101970.0 38 105111.0 39 106920.5 40 108699.5 41 113518.5 42 118120.0 43 121873.0 44 128315.5 45 132706.5 46 132165.5 47 132478.0 48 136247.0 49 133047.0 50 125503.0 51 118089.0 52 109418.0 53 101831.5 54 96829.5 55 89701.0 56 78352.5 57 68328.5 58 60748.5 59 52997.5 60 43080.0 61 34677.5 62 27915.5 63 21209.0 64 15242.0 65 10918.5 66 7930.5 67 6139.0 68 4549.5 69 2974.0 70 2200.0 71 1545.0 72 1104.5 73 873.0 74 667.0 75 477.0 76 296.0 77 186.5 78 117.0 79 75.0 80 44.5 81 29.5 82 24.0 83 17.5 84 13.5 85 7.0 86 2.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022214154492945677 2 0.0 3 0.0 4 1.772405943586091E-4 5 0.023868400040292694 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.9540099059768186E-5 12-13 0.0035005017385825303 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010191334175620025 24-25 1.4770049529884093E-5 26-27 5.021816840160592E-4 28-29 0.0013883846558091048 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.6247054482872503E-4 40-41 7.532725260240887E-4 42-43 0.015257461164370269 44-45 1.4770049529884093E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.067806934183773E-4 70-71 1.4770049529884093E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1816039623907274E-4 82-83 0.002259817578072266 84-85 4.8741163448617507E-4 86-87 0.0 88-89 0.0 90-91 0.011210467593182026 92-93 0.0025995287172596005 94-95 0.0 96-97 0.0 98-99 0.001595165349227482 100-101 0.01923060448790909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3385229.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.88785336289386 #Duplication Level Percentage of deduplicated Percentage of total 1 69.35491559141586 32.519031122461875 2 15.412990765045297 14.453641017501623 3 6.026546590817283 8.47715498504666 4 2.987383920014195 5.602880767211704 5 1.6943176845347085 3.972145957131063 6 1.0485946990675281 2.9499812692191636 7 0.690839169418618 2.267437597113048 8 0.49546829025003497 1.85851556313659 9 0.3494005288868078 1.4744376687025982 >10 1.7549217531274537 14.663264520126592 >50 0.11715758676147735 3.819605341377183 >100 0.06348413199683646 5.2894430616358825 >500 0.0030318389832070967 0.9315984163300558 >1k 7.579597444427737E-4 0.5882906572668816 >5k 6.316331203689782E-5 0.2637001617148768 >10k+ 1.2632662407379563E-4 0.8688718940241529 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16525 0.4881501369626693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12813 0.3784972892528098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8904 0.26302504202817595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9540099059768186E-5 0.0 4 0.0 0.0 0.0 2.9540099059768186E-5 0.0 5 0.0 0.0 0.0 2.9540099059768186E-5 0.0 6 0.0 0.0 0.0 2.9540099059768186E-5 0.0 7 0.0 0.0 0.0 2.9540099059768186E-5 0.0 8 0.0 0.0 0.0 2.9540099059768186E-5 0.0 9 0.0 0.0 0.0 2.9540099059768186E-5 0.0 10-11 1.4770049529884093E-5 0.0 0.0 1.1816039623907274E-4 0.0 12-13 2.9540099059768186E-5 0.0 0.0 1.1816039623907274E-4 0.0 14-15 2.9540099059768186E-5 0.0 0.0 1.3293044576895684E-4 0.0 16-17 4.4310148589652275E-5 0.0 0.0 1.4770049529884093E-4 0.0 18-19 5.908019811953637E-5 0.0 0.0 1.7724059435860913E-4 0.0 20-21 5.908019811953637E-5 0.0 0.0 2.215507429482614E-4 0.0 22-23 5.908019811953637E-5 0.0 0.0 3.1017104012756597E-4 0.0 24-25 5.908019811953637E-5 0.0 0.0 5.021816840160592E-4 0.0 26-27 5.908019811953637E-5 0.0 0.0 5.908019811953637E-4 0.0 28-29 5.908019811953637E-5 0.0 0.0 0.0011668339128608433 0.0 30-31 5.908019811953637E-5 0.0 0.0 0.0022745876276021506 0.0 32-33 5.908019811953637E-5 0.0 0.0 0.004667335651443373 0.0 34-35 5.908019811953637E-5 0.0 0.0 0.00725209431917309 0.0 36-37 5.908019811953637E-5 0.0 0.0 0.011033226998823418 0.0 38-39 5.908019811953637E-5 0.0 0.0 0.01447464853928641 0.0 40-41 5.908019811953637E-5 0.0 0.0 0.019009053744960827 0.0 42-43 5.908019811953637E-5 0.0 0.0 0.027043960689217775 0.0 44-45 5.908019811953637E-5 0.0 0.0 0.03854982927299748 0.0 46-47 5.908019811953637E-5 0.0 0.0 0.06296472114589588 0.0 48-49 5.908019811953637E-5 0.0 0.0 0.10178041131043128 0.0 50-51 5.908019811953637E-5 0.0 0.0 0.13423021012758662 0.0 52-53 5.908019811953637E-5 0.0 0.0 0.1663846079541443 0.0 54-55 5.908019811953637E-5 0.0 0.0 0.20472765653372343 0.0 56-57 5.908019811953637E-5 0.0 0.0 0.2431002452123623 0.0 58-59 5.908019811953637E-5 0.0 0.0 0.2913687670760235 0.0 60-61 5.908019811953637E-5 0.0 0.0 0.36068460951976955 0.0 62-63 5.908019811953637E-5 0.0 0.0 0.4140783385703006 0.0 64-65 5.908019811953637E-5 0.0 0.0 0.4797459787801652 0.0 66-67 5.908019811953637E-5 0.0 0.0 0.5841554589069159 0.0 68-69 5.908019811953637E-5 0.0 0.0 0.6960533541453178 0.0 70-71 5.908019811953637E-5 0.0 0.0 0.7976269847623307 0.0 72-73 7.385024764942047E-5 0.0 0.0 0.9143842262960644 0.0 74-75 8.862029717930456E-5 0.0 0.0 1.0296792329263398 0.0 76-77 8.862029717930456E-5 0.0 0.0 1.1383572573672267 0.0 78-79 8.862029717930456E-5 0.0 0.0 1.2703424199662712 0.0 80-81 8.862029717930456E-5 0.0 0.0 1.4392231662909658 0.0 82-83 8.862029717930456E-5 0.0 0.0 1.6082073029623698 0.0 84-85 8.862029717930456E-5 0.0 0.0 1.8070860198822591 0.0 86-87 8.862029717930456E-5 0.0 0.0 2.089371206497404 0.0 88-89 8.862029717930456E-5 0.0 0.0 2.47212227001482 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17560 0.0 34.988434 1 GGTATCA 11640 0.0 34.290737 1 ATCAACG 21730 0.0 28.114834 3 TCAACGC 21880 0.0 27.900377 4 TATCAAC 21940 0.0 27.867382 2 CAACGCA 22140 0.0 27.57273 5 AACGCAG 22725 0.0 26.961885 6 GTACATG 14515 0.0 24.585182 1 ACGCAGA 25600 0.0 23.732304 7 TACATGG 14765 0.0 23.455696 2 CGCAGAG 25625 0.0 23.412556 8 GGACCGA 855 0.0 23.331766 6 ACATGGG 14980 0.0 22.199362 3 GAGTACT 14575 0.0 22.047968 12-13 GCAGAGT 27330 0.0 21.534807 9 AGTACTT 15045 0.0 21.090828 12-13 GTACTTT 15425 0.0 20.584518 14-15 AGAGTAC 22700 0.0 20.486425 10-11 AGGACCG 1145 0.0 20.33035 5 GTGTAGG 1585 0.0 19.186739 1 >>END_MODULE