FastQCFastQC Report
Thu 26 May 2016
SRR937829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166816
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144010.45474697614259874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102010.32212165152632805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71520.22584198134656389No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44900.1417827875064418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149600.037.3779831
GGTATCA95000.035.8368261
ATCAACG182750.030.3212743
TCAACGC183500.030.2740824
TATCAAC184850.030.1358342
CAACGCA185400.029.9135115
AACGCAG191600.028.9827566
ACGCAGA214850.025.4713257
CGCAGAG216100.025.2580418
GTACATG133250.024.9430791
TACATGG133000.024.6230952
GCAGAGT228200.023.126999
ACATGGG135750.022.9652023
GAGTACT121300.022.18396412-13
AGTACTT126400.021.25130312-13
AGAGTAC190950.020.9585210-11
GTACTTT129500.020.63156714-15
CATGGGG67700.020.2862474
CAGAGTA219950.019.02664810-11
GTATAGA10650.018.3051951
Produced by FastQC (version 0.11.5)