##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937829_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3166816 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.809780865070785 33.0 31.0 34.0 30.0 34.0 2 31.947340167537362 33.0 31.0 34.0 30.0 34.0 3 31.971504501682446 34.0 31.0 34.0 30.0 34.0 4 35.56111090761193 37.0 35.0 37.0 33.0 37.0 5 35.55149083495852 37.0 35.0 37.0 33.0 37.0 6 35.60433065893314 37.0 35.0 37.0 33.0 37.0 7 35.550126057213305 37.0 35.0 37.0 33.0 37.0 8 35.536402493861345 37.0 35.0 37.0 33.0 37.0 9 37.17145770388933 39.0 37.0 39.0 34.0 39.0 10-11 37.06923420874468 39.0 37.0 39.0 33.0 39.0 12-13 36.95157138905449 39.0 37.0 39.0 33.0 39.0 14-15 38.12506347069107 40.0 38.0 41.0 33.0 41.0 16-17 38.060465622252764 40.0 38.0 41.0 33.0 41.0 18-19 38.00354409602579 40.0 38.0 41.0 33.0 41.0 20-21 37.92533225801562 40.0 38.0 41.0 33.0 41.0 22-23 37.73279202201833 40.0 37.5 41.0 32.5 41.0 24-25 37.57452832750624 40.0 37.0 41.0 32.0 41.0 26-27 37.522339157058695 40.0 37.0 41.0 32.0 41.0 28-29 37.568855910794944 40.0 37.0 41.0 32.0 41.0 30-31 37.39814090872346 40.0 37.0 41.0 32.0 41.0 32-33 37.1740232144842 39.5 36.5 41.0 31.5 41.0 34-35 37.199274918403844 40.0 37.0 41.0 31.0 41.0 36-37 37.12097276886311 40.0 36.5 41.0 31.0 41.0 38-39 37.00051565989309 40.0 36.0 41.0 31.0 41.0 40-41 36.78268724801188 39.5 36.0 41.0 30.5 41.0 42-43 36.542662724957815 39.0 36.0 41.0 30.0 41.0 44-45 36.315803949455855 39.0 36.0 41.0 30.0 41.0 46-47 35.9764162174247 39.0 35.0 41.0 29.0 41.0 48-49 35.631923989268714 39.0 35.0 40.5 27.5 41.0 50-51 34.716213540666715 38.0 34.0 39.5 25.5 40.5 52-53 34.82039783808089 38.0 34.5 39.5 26.0 40.5 54-55 35.18795392596223 39.0 35.0 40.0 26.0 41.0 56-57 35.0011423145519 38.5 35.0 40.0 26.0 41.0 58-59 34.77827619287007 38.0 34.0 40.0 25.5 41.0 60-61 34.47616533451897 38.0 34.0 40.0 24.5 41.0 62-63 34.12793338798339 38.0 34.0 40.0 23.0 41.0 64-65 33.77452258040884 37.0 33.5 40.0 22.5 41.0 66-67 33.37857677869506 37.0 33.0 39.5 22.0 41.0 68-69 32.90552419212231 36.0 32.0 39.0 20.0 40.5 70-71 32.47231967376696 36.0 32.0 39.0 18.5 40.0 72-73 32.02173729070461 35.0 31.5 38.0 17.5 40.0 74-75 31.565685060325578 35.0 31.0 37.5 14.0 39.5 76-77 31.06505540580823 35.0 31.0 37.0 9.0 39.0 78-79 30.534548581287957 34.5 30.0 36.0 7.0 39.0 80-81 30.004784142810948 34.0 29.5 36.0 3.5 37.5 82-83 29.47606191834322 34.0 29.0 35.0 2.0 37.0 84-85 29.004503419207175 34.0 29.0 35.0 2.0 37.0 86-87 28.537092303436637 34.0 28.5 35.0 2.0 36.0 88-89 28.061762192688178 34.0 27.0 35.0 2.0 36.0 90-91 27.559970487707528 33.0 26.5 35.0 2.0 35.0 92-93 27.003775874569286 33.0 25.5 35.0 2.0 35.0 94-95 26.383948262229318 32.5 25.0 35.0 2.0 35.0 96-97 25.665221945322997 32.0 23.0 34.0 2.0 35.0 98-99 24.742243471044734 32.0 13.0 34.0 2.0 35.0 100-101 22.35667386422198 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07581145152588675 1101 2 0.22774003136147059 1101 3 0.1451285042894881 1101 4 0.122492151845087 1101 5 0.12043809708421094 1101 6 0.1396214548478838 1101 7 0.16970561742147794 1101 8 0.06570227869092093 1101 9 0.21869577218443226 1101 10-11 0.22400679951098823 1101 12-13 0.3686219724551947 1101 14-15 0.469974894937053 1101 16-17 0.48948273159415123 1101 18-19 0.427424834963702 1101 20-21 0.4755837784264898 1101 22-23 0.4438150270292809 1101 24-25 0.483672336325391 1101 26-27 0.4918534513781907 1101 28-29 0.3148259876448307 1101 30-31 0.3294485060714081 1101 32-33 0.38729246544702534 1101 34-35 0.5186077634514064 1101 36-37 0.3825285816209387 1101 38-39 0.5048001827345772 1101 40-41 0.6200282191068638 1101 42-43 0.534698645480816 1101 44-45 0.5660000850150837 1101 46-47 0.6523943450212428 1101 48-49 0.6766435568685338 1101 50-51 0.44005670940588004 1101 52-53 0.4754571365746685 1101 54-55 0.5891804127301512 1101 56-57 0.5481714425527286 1101 58-59 0.5406804902593194 1101 60-61 0.6427850875544792 1101 62-63 0.7223620341983263 1101 64-65 0.5135283024979245 1101 66-67 0.5750438733146837 1101 68-69 0.6710446871082141 1101 70-71 0.6805051702100755 1101 72-73 0.8351054642773441 1101 74-75 0.8338407480099441 1101 76-77 0.8774199092460044 1101 78-79 0.9497144042081942 1101 80-81 0.932933093405726 1101 82-83 0.9159049037016587 1101 84-85 1.1026933469500477 1101 86-87 1.2176009388734457 1101 88-89 1.184626764619619 1101 90-91 1.2461414220764055 1101 92-93 1.2815444999835854 1101 94-95 1.458590074779334 1101 96-97 1.4384091323134207 1101 98-99 1.7807203932110234 1101 100-101 2.3656316564895086 1102 1 0.351810719948336 1102 2 0.20449450483565812 1102 3 0.26658989169655456 1102 4 0.265045637514568 1102 5 0.27443009523601347 1102 6 0.19831847673220437 1102 7 0.21628978034274837 1102 8 0.3036294989762709 1102 9 0.3824927471637807 1102 10-11 0.2750413341716609 1102 12-13 0.34733166568267393 1102 14-15 0.4538016493230117 1102 16-17 0.4571050433091912 1102 18-19 0.4390258084168863 1102 20-21 0.44968816474143836 1102 22-23 0.5882706580761692 1102 24-25 0.5842480160974404 1102 26-27 0.47467389284498296 1102 28-29 0.33058618947622875 1102 30-31 0.5528649382132187 1102 32-33 0.4886462777011289 1102 34-35 0.6313349375318609 1102 36-37 0.6601888636108342 1102 38-39 0.6535412742902835 1102 40-41 0.6474708217727496 1102 42-43 0.6908160689276954 1102 44-45 0.767508307386656 1102 46-47 0.8581857964150927 1102 48-49 0.8049460256802234 1102 50-51 0.7406812631085771 1102 52-53 0.741829640810117 1102 54-55 0.7576935688747 1102 56-57 0.8751399637159665 1102 58-59 0.9216741755899704 1102 60-61 0.9389326229432342 1102 62-63 0.988001692002058 1102 64-65 0.9915761193165125 1102 66-67 1.1649008043993803 1102 68-69 1.0270259461216682 1102 70-71 1.109679625202304 1102 72-73 1.1623953195020071 1102 74-75 1.1112940268075029 1102 76-77 1.095738751043232 1102 78-79 1.344973414163249 1102 80-81 1.3131584035386048 1102 82-83 1.329943710565189 1102 84-85 1.3630439878679823 1102 86-87 1.3858699441380047 1102 88-89 1.5325195412539436 1102 90-91 1.5542039142005706 1102 92-93 1.624130626613013 1102 94-95 1.7900463341428257 1102 96-97 1.7976606129703718 1102 98-99 2.242459762584179 1102 100-101 2.8213036139670002 1103 1 0.4257701187436602 1103 2 0.38735974520388083 1103 3 0.394149131382882 1103 4 0.2857511154097807 1103 5 0.2960248787341371 1103 6 0.2875700962344183 1103 7 0.30726176579564424 1103 8 0.34374769422573337 1103 9 0.3362850815199536 1103 10-11 0.32022402538656536 1103 12-13 0.44087780728967374 1103 14-15 0.5547239842474383 1103 16-17 0.48925962053387906 1103 18-19 0.4023941599341043 1103 20-21 0.40604790732582074 1103 22-23 0.5045299631108335 1103 24-25 0.564703536611141 1103 26-27 0.46444758871951564 1103 28-29 0.42782557747487004 1103 30-31 0.4883618696827057 1103 32-33 0.4867117538916048 1103 34-35 0.522241574649712 1103 36-37 0.6177721117072039 1103 38-39 0.6925783807796577 1103 40-41 0.6931127271028998 1103 42-43 0.7250813263433145 1103 44-45 0.6828019487095389 1103 46-47 0.727853514337589 1103 48-49 0.8519318337265958 1103 50-51 0.8555700288774091 1103 52-53 0.6866348033285874 1103 54-55 0.7569907191190381 1103 56-57 0.7978847358491663 1103 58-59 0.8924748347557809 1103 60-61 0.852377687723326 1103 62-63 0.9651723932181113 1103 64-65 1.0641372768344084 1103 66-67 0.9955758100818457 1103 68-69 1.0649322422916896 1103 70-71 1.0333435358739749 1103 72-73 1.0217323180245579 1103 74-75 0.9820879380474103 1103 76-77 0.9371113502022261 1103 78-79 1.058575101855034 1103 80-81 0.980664299094915 1103 82-83 1.2426472138041902 1103 84-85 1.1710480934481637 1103 86-87 1.3577634121457294 1103 88-89 1.285990106090857 1103 90-91 1.43914893636218 1103 92-93 1.5005974126114197 1103 94-95 1.629019512913139 1103 96-97 1.8439272341773254 1103 98-99 2.027870036478941 1103 100-101 2.397624431673556 1104 1 0.16799870242730464 1104 2 0.2084275215760485 1104 3 0.24807842062360663 1104 4 0.17908306487166215 1104 5 0.11252424910885139 1104 6 0.15093362115062803 1104 7 0.19625009780306613 1104 8 0.22482821525695584 1104 9 0.3044487927878592 1104 10-11 0.3643649962412212 1104 12-13 0.42005479865171225 1104 14-15 0.47380961835762037 1104 16-17 0.5225885522865568 1104 18-19 0.4696840940444318 1104 20-21 0.4091363497531475 1104 22-23 0.51920108352504 1104 24-25 0.5883894745934128 1104 26-27 0.49795919512619236 1104 28-29 0.46213082691226504 1104 30-31 0.5876576348911655 1104 32-33 0.5402181291192889 1104 34-35 0.49248616487160746 1104 36-37 0.5193288584065598 1104 38-39 0.5846378568212955 1104 40-41 0.6910899019184669 1104 42-43 0.6735715117241199 1104 44-45 0.6772017977153908 1104 46-47 0.708228623199723 1104 48-49 0.7330639782346537 1104 50-51 0.6839520368096785 1104 52-53 0.768815665183439 1104 54-55 0.6705467543200996 1104 56-57 0.7623466815579292 1104 58-59 0.7739120106146871 1104 60-61 0.8602015426240399 1104 62-63 0.8949320415553572 1104 64-65 0.7454411011015694 1104 66-67 0.7492546620626683 1104 68-69 0.8305355740165439 1104 70-71 0.7816595237190924 1104 72-73 0.9265818274385076 1104 74-75 1.0177709003438018 1104 76-77 1.0860067708194485 1104 78-79 1.0877074977784815 1104 80-81 1.105002069511368 1104 82-83 1.1584281302893622 1104 84-85 1.2058021954315166 1104 86-87 1.3870818337207957 1104 88-89 1.440868053700818 1104 90-91 1.4595681045032833 1104 92-93 1.3378335713610348 1104 94-95 1.4206138161447335 1104 96-97 1.521843109284017 1104 98-99 1.7094788015294462 1104 100-101 2.210139647014099 1105 1 0.3054752364157203 1105 2 0.21202675782495817 1105 3 0.29519671989038443 1105 4 0.2363194994822564 1105 5 0.2798659039207223 1105 6 0.2443851853539769 1105 7 0.3730209529880213 1105 8 0.23324980524856187 1105 9 0.31627054242284913 1105 10-11 0.4140569624494006 1105 12-13 0.42914937073826565 1105 14-15 0.5085956759645143 1105 16-17 0.564104335316749 1105 18-19 0.5005562520921529 1105 20-21 0.5031278280516958 1105 22-23 0.49980372394568917 1105 24-25 0.5076124224326648 1105 26-27 0.5228685121023844 1105 28-29 0.5815400126354504 1105 30-31 0.5235225845316549 1105 32-33 0.5714348501785764 1105 34-35 0.598219246321456 1105 36-37 0.5558412655407707 1105 38-39 0.6122862921212047 1105 40-41 0.762184609270129 1105 42-43 0.7445841620680369 1105 44-45 0.7479873822765271 1105 46-47 0.7264585570611999 1105 48-49 0.7467864127160979 1105 50-51 0.6656930629827045 1105 52-53 0.7011443303423235 1105 54-55 0.7583314176265361 1105 56-57 0.8211109622475448 1105 58-59 0.7632621764290661 1105 60-61 0.7566619338305856 1105 62-63 0.7499064539068172 1105 64-65 0.787278998485796 1105 66-67 0.906637614762289 1105 68-69 0.9215062272208883 1105 70-71 1.0419522809606434 1105 72-73 0.9799522211172693 1105 74-75 1.0461593771326498 1105 76-77 1.0265967330025383 1105 78-79 1.109986787353936 1105 80-81 1.1770789772747072 1105 82-83 1.2391063128307636 1105 84-85 1.3933388536116382 1105 86-87 1.4854300710522068 1105 88-89 1.1788484693009345 1105 90-91 1.4190992886499103 1105 92-93 1.7164777526881103 1105 94-95 1.9147447818038295 1105 96-97 1.977553627444884 1105 98-99 2.1161827539833844 1105 100-101 2.4136015738998715 1106 1 0.2853226247102434 1106 2 0.3121730762642301 1106 3 0.35926846312512595 1106 4 0.2926646851336159 1106 5 0.3405015238074398 1106 6 0.1386756195893497 1106 7 0.26295644700941523 1106 8 0.09446283230960262 1106 9 0.2567189376399668 1106 10-11 0.13260085798118126 1106 12-13 0.17881976092076712 1106 14-15 0.18252188741825393 1106 16-17 0.09844432902347933 1106 18-19 0.37822223698831436 1106 20-21 0.29977745045572846 1106 22-23 0.3877647056952185 1106 24-25 0.3117777780022095 1106 26-27 0.41485246427355804 1106 28-29 0.562461189476231 1106 30-31 0.5921506524989368 1106 32-33 0.6576046110344649 1106 34-35 0.4607396994366155 1106 36-37 0.4301293398013044 1106 38-39 0.45217222667123735 1106 40-41 0.6933339170108468 1106 42-43 0.6484946403562688 1106 44-45 0.5467940216723761 1106 46-47 0.6893762726055712 1106 48-49 0.7291424542516509 1106 50-51 0.7296098345371433 1106 52-53 0.6204308312863134 1106 54-55 0.6770090450651765 1106 56-57 0.6865090113350192 1106 58-59 0.5778051279709189 1106 60-61 0.6277719086575217 1106 62-63 0.8425850253353886 1106 64-65 0.8064868336022286 1106 66-67 1.004573423446999 1106 68-69 0.9041092794549996 1106 70-71 0.9716439109165904 1106 72-73 0.9782286528353374 1106 74-75 1.265073788712261 1106 76-77 1.2106197034241823 1106 78-79 1.1024734141632493 1106 80-81 1.174438165443366 1106 82-83 0.8875329962794751 1106 84-85 0.9868237497727463 1106 86-87 1.2205425631856244 1106 88-89 1.3978171603015639 1106 90-91 1.5834896284862836 1106 92-93 1.5427615789939644 1106 94-95 1.7270701436666336 1106 96-97 1.761589184398943 1106 98-99 1.8951978578222715 1106 100-101 2.2834167092050954 1107 1 0.14363161716183726 1107 2 0.21031258116366303 1107 3 0.24911749495971947 1107 4 0.21732207647873025 1107 5 0.16541830377837385 1107 6 0.163023807502519 1107 7 0.1924394279274182 1107 8 0.30605186601957257 1107 9 0.2613500736519683 1107 10-11 0.3258944371457062 1107 12-13 0.20763961886066085 1107 14-15 0.46017232813277076 1107 16-17 0.4611996738591486 1107 18-19 0.4485824859451384 1107 20-21 0.3695556280543002 1107 22-23 0.4757121012599441 1107 24-25 0.50289345384261 1107 26-27 0.41907851800322504 1107 28-29 0.34429771486420435 1107 30-31 0.31592813855078816 1107 32-33 0.4209503492312834 1107 34-35 0.4998064629979666 1107 36-37 0.4607042026640755 1107 38-39 0.5105643805462421 1107 40-41 0.5403323112159129 1107 42-43 0.5600639239607546 1107 44-45 0.5674804697008824 1107 46-47 0.7199144483287654 1107 48-49 0.743159109978599 1107 50-51 0.658819420077954 1107 52-53 0.6648646420603725 1107 54-55 0.7750912922403828 1107 56-57 0.8609936332113364 1107 58-59 0.8714506926950065 1107 60-61 0.9357179562922013 1107 62-63 1.0037978693505778 1107 64-65 0.9777889926345225 1107 66-67 0.9727355501289807 1107 68-69 1.0643731807161956 1107 70-71 1.167851890662476 1107 72-73 1.1678209332985539 1107 74-75 1.0944771831517635 1107 76-77 1.1372729689312457 1107 78-79 1.2878789066851688 1107 80-81 1.1621765146392953 1107 82-83 1.2682892436422186 1107 84-85 1.3374661849548914 1107 86-87 1.396056241745331 1107 88-89 1.5015765372062475 1107 90-91 1.5742678529693848 1107 92-93 1.631285888619356 1107 94-95 1.7588280788722237 1107 96-97 1.911289943928221 1107 98-99 2.1571344230620895 1107 100-101 2.44072313472903 1108 1 0.28733447966142833 1108 2 0.26387510854157625 1108 3 0.25092815629109566 1108 4 0.2503639280106782 1108 5 0.23458401035854592 1108 6 0.2598745636020965 1108 7 0.31398603457665786 1108 8 0.24933581497547408 1108 9 0.33956462403168075 1108 10-11 0.353082278582896 1108 12-13 0.4164041900899278 1108 14-15 0.4225734415126965 1108 16-17 0.5031656380491825 1108 18-19 0.5464033985742844 1108 20-21 0.6007161334603026 1108 22-23 0.5426023227504047 1108 24-25 0.65073847734471 1108 26-27 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-0.7822625268396344 2316 100-101 -0.5043882684167755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12324.0 3 2136.0 4 1994.0 5 2389.0 6 2698.0 7 3055.0 8 3716.0 9 4808.0 10 6072.0 11 7984.0 12 10656.0 13 14134.0 14 15675.0 15 20057.0 16 25123.0 17 32858.0 18 28393.0 19 19819.0 20 19522.0 21 20481.0 22 22318.0 23 24568.0 24 27421.0 25 31647.0 26 36775.0 27 43471.0 28 51205.0 29 62027.0 30 75780.0 31 94767.0 32 121674.0 33 157859.0 34 217517.0 35 305668.0 36 445595.0 37 626615.0 38 507498.0 39 60514.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10931945624415 19.218317707036555 15.141162510318804 22.53120032640049 2 14.729535555356382 20.075546799015555 41.76014477216721 23.434772873460854 3 18.761649141628865 24.85960864584013 30.308034495708934 26.07070771682207 4 12.271128377073419 16.580674575659426 38.42909293541412 32.71910411185304 5 13.16868753834844 37.64592985742138 34.765578251710124 14.419804352520057 6 30.91689306685359 36.57562662877595 18.12893497238102 14.378545331989438 7 27.732209788196133 33.122062125126774 21.33594495346074 17.80978313321636 8 25.604971169243818 35.1348811008222 20.38638276863872 18.873764961295265 9 25.944718705564874 17.145973973693927 21.16891464436232 35.740392676378875 10-11 24.20572818179488 27.002725821821194 28.0119413339089 20.779604662475027 12-13 25.10609196277133 24.37804135699607 28.705837079392182 21.810029600840412 14-15 22.21982594254224 25.71869666717405 27.2383212483833 24.823156141900405 16-17 21.157118395357514 29.070869874377813 27.842907237302416 21.929104492962253 18-19 21.05765322243901 27.623575338713806 30.430010327513667 20.88876111133351 20-21 21.899900030074825 26.55529646801935 30.62996873388778 20.91483476801804 22-23 21.588644063292147 26.447681975051157 30.505649367167102 21.45802459448959 24-25 21.37938464108493 26.57174441546345 30.644697427019146 21.40417351643248 26-27 21.183491587341894 26.81814118583316 30.59552711914419 21.402840107680756 28-29 21.004301463554658 27.02046387699488 30.685957081574948 21.289277577875513 30-31 21.27560595816336 26.671573942932657 30.6216728927998 21.43114720610418 32-33 21.116555130467365 26.73567875042745 30.497194940147697 21.650571178957488 34-35 21.493752219144167 26.74730902069049 30.427544033199975 21.331394726965364 36-37 21.4603483873846 26.653696282605456 30.350493041760195 21.53546228824975 38-39 21.229777568880177 26.975470545622986 30.320804592555024 21.473947292941812 40-41 21.50544285300951 26.801176762965344 30.23773655525216 21.45564382877298 42-43 21.21291996197126 26.750772053338327 30.118046358165984 21.91826162652443 44-45 21.423203636013852 26.9379290617849 29.912931466555964 21.725935835645288 46-47 21.52290770580895 27.143640726448666 29.51637116765866 21.817080400083725 48-49 21.592965861739692 27.083062040004524 29.475137400346675 21.84883469790911 50-51 21.66650315058565 27.326496251261972 29.274637389903567 21.732363208248806 52-53 21.63331885768113 27.498126183796046 29.047696218942765 21.82085873958006 54-55 21.523242253043794 27.32979859725479 29.155560295117922 21.991398854583494 56-57 21.611014767466653 27.579672257269376 29.046311802901357 21.76300117236261 58-59 21.726778538903787 27.309919376319552 29.1107606008783 21.852541483898356 60-61 21.735808561385213 27.322756699348627 29.341411707610604 21.600023031655553 62-63 21.544890752404704 27.37685539932158 29.369199825474684 21.70905402279903 64-65 21.474204801002724 27.302229004214034 29.277593965552835 21.94597222923041 66-67 21.498099992501842 27.361500036225273 29.09083747445644 22.049562496816446 68-69 21.41164574359419 27.37089704684029 29.185673080558512 22.031784129007008 70-71 21.550941949297854 27.285905371015524 29.11239928475886 22.05075339492776 72-73 21.59659228962501 27.342911613144892 28.867747479144732 22.192748618085364 74-75 21.367339787979045 27.60524523672615 28.819473720427524 22.207941254867283 76-77 21.48460058280808 27.4009822743185 28.811629074838844 22.30278806803457 78-79 21.52646801939216 27.3540208351793 28.822293388691282 22.297217756737258 80-81 21.478837419865798 27.679584381921096 28.67232217172407 22.16925602648904 82-83 21.61839249213712 27.562254383911 28.748584048955518 22.07076907499636 84-85 21.542337738516537 27.381297579324382 28.658479578233802 22.417885103925276 86-87 21.37602204622018 27.385958348526756 28.902711719885183 22.33530788536788 88-89 21.59568892128266 27.217465551602317 28.790344181010376 22.39650134610465 90-91 21.552883283547946 27.20141146757284 28.84101482771872 22.404690421160495 92-93 21.58283613186805 26.993127403281044 28.964589088578062 22.459447376272852 94-95 21.368238820534184 27.096687233459228 28.974165765046873 22.56090818095972 96-97 21.282838171535555 27.16305753057678 28.963963686078475 22.59014061180919 98-99 21.264290628460984 27.212564758581337 28.870719811534318 22.65242480142336 100-101 21.42709584593887 26.80457541793223 29.21038070800217 22.557948028126727 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4236.0 1 2475.0 2 671.5 3 616.0 4 526.0 5 518.0 6 826.5 7 1726.5 8 2977.5 9 3612.5 10 3442.0 11 3343.5 12 3573.5 13 3845.0 14 4182.5 15 4488.5 16 4747.0 17 5054.5 18 5362.5 19 5752.5 20 6252.5 21 7138.5 22 8560.0 23 10374.5 24 12638.5 25 16072.0 26 20637.5 27 25767.5 28 30318.5 29 35464.5 30 42164.5 31 49078.5 32 56124.0 33 63660.0 34 71911.5 35 79881.0 36 88993.0 37 96052.0 38 99013.0 39 100876.5 40 102361.0 41 106530.5 42 110761.5 43 113928.0 44 119469.0 45 124634.0 46 125414.5 47 125108.5 48 127300.5 49 123882.0 50 116331.5 51 108865.5 52 100790.0 53 94509.0 54 89943.5 55 83149.5 56 72849.5 57 63569.5 58 56814.0 59 49432.0 60 40384.5 61 32727.5 62 25730.0 63 19172.5 64 14283.0 65 10666.5 66 7540.0 67 5497.5 68 4051.0 69 2782.0 70 2054.5 71 1469.5 72 1123.0 73 875.5 74 596.5 75 431.5 76 315.0 77 195.5 78 119.5 79 84.0 80 59.0 81 42.5 82 34.0 83 22.5 84 15.5 85 11.5 86 7.5 87 8.0 88 8.0 89 4.0 90 2.0 91 3.5 92 3.5 93 3.0 94 2.0 95 3.5 96 3.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3144483291735295 2 0.29376509402503964 3 0.27971312510736335 4 0.26480856481715387 5 0.26139819932702124 6 0.21633716641573114 7 0.20781125269039943 8 0.2209158978608167 9 0.22145271465093017 10-11 0.2221474187322535 12-13 0.21913177147014543 14-15 0.2155161525014399 16-17 0.21425305417176113 18-19 0.2150582793569314 20-21 0.2010063104392551 22-23 0.192875114941948 24-25 0.1933803542738195 26-27 0.20678498529753545 28-29 0.22348946070753717 30-31 0.21733185635035315 32-33 0.22355261562402112 34-35 0.21516880046077827 36-37 0.19838538140517165 38-39 0.1992379727777048 40-41 0.19298563604579488 42-43 0.20760599921182663 44-45 0.20310621141234603 46-47 0.20296411285025717 48-49 0.21467934985802775 50-51 0.22375786910259388 52-53 0.23645200731586552 54-55 0.21016377332942615 56-57 0.17929680789790123 58-59 0.18030728656164424 60-61 0.1878542990814749 62-63 0.19713807180461387 64-65 0.18411237027980154 66-67 0.19069627032325212 68-69 0.19421715691723168 70-71 0.18706486262542568 72-73 0.1889595101199438 74-75 0.190838368885341 76-77 0.2083796469387549 78-79 0.21396885704758345 80-81 0.20255360589311158 82-83 0.20196942291563513 84-85 0.19285932621282703 86-87 0.19525921303921667 88-89 0.202111521477724 90-91 0.20264833826783746 92-93 0.1987169447167123 94-95 0.19895377565352707 96-97 0.18565966573365805 98-99 0.1937119175853602 100-101 0.18431762375837435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3166816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64330629285317 #Duplication Level Percentage of deduplicated Percentage of total 1 71.43113686946681 35.46077806457657 2 14.716464830643133 14.611479422712373 3 5.66650748433531 8.43912499966808 4 2.696795204295232 5.355113213437029 5 1.5344204635895557 3.8086852527999038 6 0.8962585124625188 2.6695941511052266 7 0.617843438124004 2.1470253727873585 8 0.42055759375126894 1.6702295552303645 9 0.32048238046256283 1.4318824477289138 >10 1.5295934355551448 13.554660732082443 >50 0.11199659657547985 3.8622806020152245 >100 0.05546493541817586 4.832104528763212 >500 0.0016521702139383283 0.5750491732815785 >1k 6.35450082188514E-4 0.580285783160498 >5k 6.354500821885141E-5 0.2256158695930351 >10k+ 1.2709001643770282E-4 0.7760908310581446 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14401 0.45474697614259874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10201 0.32212165152632805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7152 0.22584198134656389 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4490 0.1417827875064418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.157745824196922E-5 8 0.0 0.0 0.0 0.0 3.157745824196922E-5 9 0.0 0.0 0.0 0.0 3.157745824196922E-5 10-11 0.0 0.0 0.0 6.315491648393844E-5 3.157745824196922E-5 12-13 0.0 0.0 0.0 1.420985620888615E-4 3.157745824196922E-5 14-15 0.0 0.0 0.0 1.736760203308307E-4 3.157745824196922E-5 16-17 0.0 0.0 0.0 1.8946474945181533E-4 3.157745824196922E-5 18-19 0.0 0.0 0.0 1.8946474945181533E-4 3.157745824196922E-5 20-21 0.0 3.157745824196922E-5 0.0 2.5261966593575374E-4 3.157745824196922E-5 22-23 0.0 3.157745824196922E-5 0.0 3.473520406616614E-4 3.157745824196922E-5 24-25 0.0 3.157745824196922E-5 0.0 4.578731445085537E-4 3.157745824196922E-5 26-27 0.0 3.157745824196922E-5 0.0 6.315491648393845E-4 3.157745824196922E-5 28-29 0.0 3.157745824196922E-5 0.0 0.0014209856208886149 3.157745824196922E-5 30-31 0.0 3.157745824196922E-5 0.0 0.0027788163252932914 3.157745824196922E-5 32-33 0.0 3.157745824196922E-5 0.0 0.004989238402231137 3.157745824196922E-5 34-35 0.0 3.157745824196922E-5 0.0 0.0072312379374109515 3.157745824196922E-5 36-37 0.0 3.157745824196922E-5 0.0 0.010831068176995442 3.157745824196922E-5 38-39 0.0 3.157745824196922E-5 0.0 0.01479403918636258 3.157745824196922E-5 40-41 0.0 3.157745824196922E-5 0.0 0.019767488859472732 3.157745824196922E-5 42-43 0.0 3.157745824196922E-5 0.0 0.027614487232602083 3.157745824196922E-5 44-45 0.0 3.157745824196922E-5 0.0 0.03838240049311359 3.157745824196922E-5 46-47 0.0 3.157745824196922E-5 0.0 0.06212864909107444 3.157745824196922E-5 48-49 0.0 3.157745824196922E-5 0.0 0.10504241484191061 3.157745824196922E-5 50-51 0.0 3.157745824196922E-5 0.0 0.1387987177025757 3.157745824196922E-5 52-53 0.0 3.157745824196922E-5 0.0 0.16993409152915737 3.157745824196922E-5 54-55 0.0 3.157745824196922E-5 0.0 0.21327415296626012 3.157745824196922E-5 56-57 0.0 3.157745824196922E-5 0.0 0.2487040611137496 3.157745824196922E-5 58-59 0.0 3.157745824196922E-5 0.0 0.29663864272505885 3.157745824196922E-5 60-61 0.0 3.157745824196922E-5 0.0 0.3657301213584875 4.7366187362953826E-5 62-63 0.0 3.157745824196922E-5 0.0 0.41911181451653645 6.315491648393844E-5 64-65 0.0 3.157745824196922E-5 0.0 0.48521922334609907 6.315491648393844E-5 66-67 0.0 3.157745824196922E-5 0.0 0.5894879904610815 6.315491648393844E-5 68-69 0.0 3.157745824196922E-5 0.0 0.7005301223689662 6.315491648393844E-5 70-71 0.0 3.157745824196922E-5 0.0 0.8023674251993169 6.315491648393844E-5 72-73 0.0 3.157745824196922E-5 0.0 0.9247616533451897 6.315491648393844E-5 74-75 0.0 3.157745824196922E-5 0.0 1.0453243889130281 6.315491648393844E-5 76-77 0.0 3.157745824196922E-5 0.0 1.153350873558805 6.315491648393844E-5 78-79 0.0 3.157745824196922E-5 0.0 1.285470958843204 6.315491648393844E-5 80-81 0.0 3.157745824196922E-5 0.0 1.4484264321008862 6.315491648393844E-5 82-83 0.0 3.157745824196922E-5 0.0 1.615676439679476 6.315491648393844E-5 84-85 0.0 3.157745824196922E-5 0.0 1.8169037923264248 6.315491648393844E-5 86-87 0.0 3.157745824196922E-5 0.0 2.1026482119580043 6.315491648393844E-5 88-89 0.0 3.157745824196922E-5 0.0 2.4967664682760224 6.315491648393844E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14960 0.0 37.377983 1 GGTATCA 9500 0.0 35.836826 1 ATCAACG 18275 0.0 30.321274 3 TCAACGC 18350 0.0 30.274082 4 TATCAAC 18485 0.0 30.135834 2 CAACGCA 18540 0.0 29.913511 5 AACGCAG 19160 0.0 28.982756 6 ACGCAGA 21485 0.0 25.471325 7 CGCAGAG 21610 0.0 25.258041 8 GTACATG 13325 0.0 24.943079 1 TACATGG 13300 0.0 24.623095 2 GCAGAGT 22820 0.0 23.12699 9 ACATGGG 13575 0.0 22.965202 3 GAGTACT 12130 0.0 22.183964 12-13 AGTACTT 12640 0.0 21.251303 12-13 AGAGTAC 19095 0.0 20.95852 10-11 GTACTTT 12950 0.0 20.631567 14-15 CATGGGG 6770 0.0 20.286247 4 CAGAGTA 21995 0.0 19.026648 10-11 GTATAGA 1065 0.0 18.305195 1 >>END_MODULE