FastQCFastQC Report
Thu 26 May 2016
SRR937829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166816
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158020.4989869951395976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122100.3855607651344442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83220.26278760748966784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32360.10218465487101241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109850.036.465841
GTATCAA167350.033.9597241
ATCAACG209050.026.9700013
TATCAAC210650.026.8553432
TCAACGC210800.026.7461054
CAACGCA214000.026.3683575
AACGCAG219300.025.6865566
GTACATG130800.024.2676331
TACATGG133650.023.4205362
ACGCAGA247050.022.6703037
CGCAGAG247950.022.5113838
ACCGTCG1503.033506E-522.1684448
ACATGGG134400.021.8408553
GAGTACT144650.021.7075812-13
AGTACTT149900.021.12160712-13
GTACCGT2257.167546E-821.1094686
GCAGAGT263150.020.8139429
AGAGTAC218250.020.60130710-11
GTACTTT151650.020.49873514-15
CATGGGG64600.018.749434
Produced by FastQC (version 0.11.5)