##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937829_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3166816 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.303529159888036 33.0 31.0 34.0 31.0 34.0 2 32.51200101300486 34.0 31.0 34.0 31.0 34.0 3 32.58059577822014 34.0 31.0 34.0 31.0 34.0 4 36.1637041747926 37.0 35.0 37.0 35.0 37.0 5 36.09054488798844 37.0 35.0 37.0 35.0 37.0 6 36.13602905884017 37.0 35.0 37.0 35.0 37.0 7 36.09195671614644 37.0 35.0 37.0 35.0 37.0 8 36.05870312642099 37.0 35.0 37.0 35.0 37.0 9 37.75934250679547 39.0 37.0 39.0 35.0 39.0 10-11 37.68275153971686 39.0 37.0 39.0 35.0 39.0 12-13 37.58248837317987 39.0 37.0 39.0 35.0 39.0 14-15 38.82851529738387 40.0 38.0 41.0 35.0 41.0 16-17 38.79916420783525 40.0 38.0 41.0 35.0 41.0 18-19 38.77526828208522 40.0 38.0 41.0 35.0 41.0 20-21 38.67150285965462 40.0 38.0 41.0 34.0 41.0 22-23 38.62141453750391 40.0 38.0 41.0 34.0 41.0 24-25 38.63910375594919 40.0 38.0 41.0 34.0 41.0 26-27 38.471815855420715 40.0 38.0 41.0 34.0 41.0 28-29 38.43536694269575 40.0 38.0 41.0 34.0 41.0 30-31 38.448993879025494 40.0 38.0 41.0 34.0 41.0 32-33 38.32703684078898 40.0 38.0 41.0 34.0 41.0 34-35 38.18815665324414 40.0 38.0 41.0 34.0 41.0 36-37 38.027214716611255 40.0 37.5 41.0 33.5 41.0 38-39 37.89457518213878 40.0 37.0 41.0 33.0 41.0 40-41 37.722598976385115 40.0 37.0 41.0 33.0 41.0 42-43 37.496199021351416 40.0 37.0 41.0 33.0 41.0 44-45 37.18921686640462 40.0 37.0 41.0 32.0 41.0 46-47 36.84113049195153 40.0 36.5 41.0 31.0 41.0 48-49 36.5046412548124 40.0 36.0 41.0 30.5 41.0 50-51 36.260673338773074 39.0 36.0 41.0 30.0 41.0 52-53 36.007407598041695 39.0 35.5 41.0 29.0 41.0 54-55 35.78988643482918 39.0 35.0 40.0 29.0 41.0 56-57 35.52347389302062 39.0 35.0 40.0 28.0 41.0 58-59 35.278851376271945 38.0 35.0 40.0 28.0 41.0 60-61 34.94398853611956 38.0 34.0 40.0 27.0 41.0 62-63 34.550744028071094 38.0 34.0 40.0 26.0 41.0 64-65 34.17048511817548 37.0 34.0 40.0 26.0 41.0 66-67 33.764014549629664 36.5 33.0 39.0 25.5 41.0 68-69 33.30406597667815 36.0 33.0 39.0 24.0 40.0 70-71 32.88988419282964 35.5 32.0 39.0 23.0 40.0 72-73 32.42252202211938 35.0 32.0 38.0 22.0 40.0 74-75 31.940104508755798 35.0 31.0 37.0 20.5 39.0 76-77 30.66236544845043 34.0 30.0 36.0 20.0 38.5 78-79 31.26012057536655 34.0 31.0 36.0 20.0 39.0 80-81 31.090023544152864 34.0 31.0 36.0 19.5 37.5 82-83 30.704229737376593 34.0 31.0 35.5 18.0 37.0 84-85 30.29372356966745 34.0 30.5 35.0 15.5 37.0 86-87 29.961981687600417 34.0 30.0 35.0 9.0 36.0 88-89 29.5721598602508 34.0 30.0 35.0 6.0 36.0 90-91 29.08922858164162 34.0 29.0 35.0 2.0 35.5 92-93 28.640095603912574 34.0 29.0 35.0 2.0 35.0 94-95 28.16931612067136 33.5 29.0 35.0 2.0 35.0 96-97 27.675583298808647 33.0 27.5 35.0 2.0 35.0 98-99 26.893572282065016 33.0 26.5 35.0 2.0 35.0 100-101 24.46012761713974 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.201218634865576 1101 2 0.22734654157339662 1101 3 0.1628332636071974 1101 4 0.14765677370866825 1101 5 0.12017382703468371 1101 6 0.12515253483784505 1101 7 0.1252163869951346 1101 8 0.10403542705023483 1101 9 0.18248464261922948 1101 10-11 0.21073833101262807 1101 12-13 0.19251842545720876 1101 14-15 0.24235095518397287 1101 16-17 0.34833683641542024 1101 18-19 0.3059577842325467 1101 20-21 0.31782886868109017 1101 22-23 0.29402015495062983 1101 24-25 0.27463394968194166 1101 26-27 0.2568993210497865 1101 28-29 0.24576095573258527 1101 30-31 0.29196777636609994 1101 32-33 0.3318504641253526 1101 34-35 0.22492924047802632 1101 36-37 0.14513150987503565 1101 38-39 0.30052799391379637 1101 40-41 0.2870641606193516 1101 42-43 0.2178838056027459 1101 44-45 0.2460861700112318 1101 46-47 0.29394841592016263 1101 48-49 0.13186586662728672 1101 50-51 0.2109434174307765 1101 52-53 0.19420859446275784 1101 54-55 0.18062189567681486 1101 56-57 0.21058027442902016 1101 58-59 0.2503574934717179 1101 60-61 0.3287605738325041 1101 62-63 0.2633348375673066 1101 64-65 0.3283994912878967 1101 66-67 0.3074215825595843 1101 68-69 0.3182293342340543 1101 70-71 0.2030039138506936 1101 72-73 0.1866169858171034 1101 74-75 0.27247170933210896 1101 76-77 0.14170553140916908 1101 78-79 0.31965780996105053 1101 80-81 0.38668360710819627 1101 82-83 0.3827552438248425 1101 84-85 0.36040160540493815 1101 86-87 0.4339732079025431 1101 88-89 0.4605464592345321 1101 90-91 0.3189756768016707 1101 92-93 0.504812104647204 1101 94-95 0.5271473152621162 1101 96-97 0.5876157405842442 1101 98-99 0.6834162293051982 1101 100-101 1.0492013846666417 1102 1 0.2234502789198416 1102 2 0.24502954097133767 1102 3 0.22882024572730586 1102 4 0.13046155101505974 1102 5 0.08839098305322324 1102 6 0.16748794739157802 1102 7 0.16044447117205607 1102 8 0.15888090317070436 1102 9 0.2390173850899302 1102 10-11 0.28097913903827276 1102 12-13 0.18514436880720098 1102 14-15 0.31587576756408 1102 16-17 0.3308284180230672 1102 18-19 0.37258721570228914 1102 20-21 0.3629842676533812 1102 22-23 0.3296601890442403 1102 24-25 0.26143396298335375 1102 26-27 0.30060604771281874 1102 28-29 0.3115663998596645 1102 30-31 0.3573953116848472 1102 32-33 0.41230535484377384 1102 34-35 0.2968876805726737 1102 36-37 0.25652631147400484 1102 38-39 0.3165378597095696 1102 40-41 0.3355652142310248 1102 42-43 0.3303116542046993 1102 44-45 0.5070668777162481 1102 46-47 0.3487199029477921 1102 48-49 0.3444812038530358 1102 50-51 0.40404195462571835 1102 52-53 0.4509436583795292 1102 54-55 0.4887519887166718 1102 56-57 0.4836882363729842 1102 58-59 0.4890442067633245 1102 60-61 0.3310588737022968 1102 62-63 0.37351133502604483 1102 64-65 0.5543080563457892 1102 66-67 0.5746603411179905 1102 68-69 0.5426579424165254 1102 70-71 0.4452481417388512 1102 72-73 0.5692067090777684 1102 74-75 0.5933098556756278 1102 76-77 0.5038452557199449 1102 78-79 0.341961649168006 1102 80-81 0.48536182324910726 1102 82-83 0.6073541013037342 1102 84-85 0.5995143576066688 1102 86-87 0.6496869423083886 1102 88-89 0.7726575242574256 1102 90-91 0.6165832607055677 1102 92-93 0.8536261789390771 1102 94-95 1.051809835739288 1102 96-97 1.0624258017007229 1102 98-99 1.1972849181362122 1102 100-101 1.4757453545774517 1103 1 0.1963087003313717 1103 2 0.19099641220670094 1103 3 0.2283208992106509 1103 4 0.16126334667365683 1103 5 0.143387289451681 1103 6 0.1851902431069945 1103 7 0.1744403797980354 1103 8 0.1390910122740152 1103 9 0.20705352556043977 1103 10-11 0.2156712915556085 1103 12-13 0.2655540744555793 1103 14-15 0.31512603748112156 1103 16-17 0.25471536540345596 1103 18-19 0.2660825702880274 1103 20-21 0.23727182873986408 1103 22-23 0.2819143514490676 1103 24-25 0.17582693377548964 1103 26-27 0.23917328546711047 1103 28-29 0.2463263867899954 1103 30-31 0.2963116658159919 1103 32-33 0.27483021562341037 1103 34-35 0.27649828973279966 1103 36-37 0.3390204159176946 1103 38-39 0.3360645814961316 1103 40-41 0.3083526048435985 1103 42-43 0.3681735561253703 1103 44-45 0.3961739495425931 1103 46-47 0.16871613831544607 1103 48-49 0.13993523271994945 1103 50-51 0.18919261095030038 1103 52-53 0.17175284124122214 1103 54-55 0.1687181070255761 1103 56-57 0.18985726126217628 1103 58-59 0.08681028813620628 1103 60-61 0.12291980960409887 1103 62-63 0.17421773632164417 1103 64-65 0.2013336416395788 1103 66-67 0.2665809428909114 1103 68-69 0.30098026597602257 1103 70-71 0.2366321058256844 1103 72-73 0.28938833483069715 1103 74-75 0.2976462859517852 1103 76-77 0.31425766053867576 1103 78-79 0.4120860951277727 1103 80-81 0.48545522874973557 1103 82-83 0.5216781722208843 1103 84-85 0.5555018988045326 1103 86-87 0.6328941959735772 1103 88-89 0.6506802399069329 1103 90-91 0.6794092920727692 1103 92-93 0.7825840150568162 1103 94-95 0.6616642936322279 1103 96-97 0.7176817256693546 1103 98-99 0.8289706920878004 1103 100-101 1.3276969257787208 1104 1 0.18525258180821425 1104 2 0.18963119766315373 1104 3 0.1857695691725283 1104 4 0.16037741535715355 1104 5 0.14125007751067642 1104 6 0.20613071779663983 1104 7 0.16645323168901172 1104 8 0.14969157625596807 1104 9 0.1518350622518696 1104 10-11 0.2753937673668432 1104 12-13 0.3019552370524963 1104 14-15 0.2981593025627802 1104 16-17 0.31721871640107935 1104 18-19 0.3410816102724965 1104 20-21 0.3456567674365374 1104 22-23 0.25543784755756604 1104 24-25 0.2904634755169653 1104 26-27 0.2933620868315643 1104 28-29 0.3106488766573037 1104 30-31 0.3165765069316109 1104 32-33 0.21251406765495062 1104 34-35 0.1820278705284366 1104 36-37 0.1887263613482375 1104 38-39 0.26598897212174677 1104 40-41 0.35980246377565095 1104 42-43 0.40982229042639773 1104 44-45 0.38946425606648916 1104 46-47 0.3213370531776434 1104 48-49 0.13439793557304114 1104 50-51 0.12696431345996473 1104 52-53 0.1960885104915988 1104 54-55 0.2950385812225207 1104 56-57 0.22489139796549296 1104 58-59 0.1748739621627493 1104 60-61 0.18048125422098593 1104 62-63 0.16919585883556465 1104 64-65 0.2842626816385305 1104 66-67 0.4015037902462737 1104 68-69 0.3819932598768361 1104 70-71 0.45141303028078994 1104 72-73 0.2998617420381322 1104 74-75 0.45319742181318645 1104 76-77 0.4272725911349866 1104 78-79 0.35686645444166487 1104 80-81 0.3196311115655277 1104 82-83 0.24945879381825975 1104 84-85 0.2769595501645661 1104 86-87 0.2832216699517218 1104 88-89 0.24023954568166062 1104 90-91 0.3034809751647565 1104 92-93 0.5181374114830959 1104 94-95 0.6092749779892657 1104 96-97 0.6693846120919105 1104 98-99 0.7912476967813689 1104 100-101 1.305547646623598 1105 1 0.1477698195114101 1105 2 0.10368015980917988 1105 3 0.09065814167863095 1105 4 0.1692070932848324 1105 5 0.19967428492022776 1105 6 0.17163888089272916 1105 7 0.14425504385928178 1105 8 0.11667275339097216 1105 9 0.1992911421698551 1105 10-11 0.11472022357781952 1105 12-13 0.18742830000083188 1105 14-15 0.22083237024921232 1105 16-17 0.25020250025507806 1105 18-19 0.2798146386097713 1105 20-21 0.29597540463680616 1105 22-23 0.2253879790589295 1105 24-25 0.21353565167644462 1105 26-27 0.20267037013782385 1105 28-29 0.18579613029809394 1105 30-31 0.13843160285329503 1105 32-33 0.07665269069441649 1105 34-35 0.09067176909794483 1105 36-37 0.11113940671210543 1105 38-39 0.18628888236533214 1105 40-41 0.13904093271224838 1105 42-43 0.1827024398140935 1105 44-45 0.17376828845674908 1105 46-47 0.09672113537909155 1105 48-49 0.023126053863528284 1105 50-51 0.029362517873046556 1105 52-53 0.1481483993067414 1105 54-55 0.0654452706549975 1105 56-57 0.03133219483742522 1105 58-59 0.055263789310004086 1105 60-61 0.10178141932847495 1105 62-63 0.0549206333268657 1105 64-65 0.21077504292016158 1105 66-67 0.20468905414504945 1105 68-69 0.17985704562608618 1105 70-71 0.24810633347369304 1105 72-73 0.20096738140838255 1105 74-75 0.2637118380544763 1105 76-77 0.10290257688629723 1105 78-79 0.12269638799746119 1105 80-81 0.267338826605517 1105 82-83 0.23782924290222596 1105 84-85 0.21612005825697622 1105 86-87 0.43010505118189357 1105 88-89 0.422714556982406 1105 90-91 0.49787259361934844 1105 92-93 0.4557239343407993 1105 94-95 0.6411447161672292 1105 96-97 0.6417622554452151 1105 98-99 0.8391095720065707 1105 100-101 1.0911081351522363 1106 1 -0.3610140067944414 1106 2 -0.3100895066477136 1106 3 -0.28338213522507516 1106 4 -0.18084797279446008 1106 5 -0.22256139789915608 1106 6 -0.3090596716560441 1106 7 -0.2395555288279425 1106 8 -0.22951195397214974 1106 9 -0.37276832919577885 1106 10-11 -0.49681848000934536 1106 12-13 -0.5217008692880327 1106 14-15 -0.46034447053115457 1106 16-17 -0.408873962929313 1106 18-19 -0.5507163557262871 1106 20-21 -0.487789541870427 1106 22-23 -0.4837624300033738 1106 24-25 -0.49707794177854936 1106 26-27 -0.5169237141919467 1106 28-29 -0.6016181239498621 1106 30-31 -0.714092783553248 1106 32-33 -0.7631113118228967 1106 34-35 -0.9722789860939969 1106 36-37 -0.8383248790021796 1106 38-39 -0.9287299974332868 1106 40-41 -0.9123514524356366 1106 42-43 -1.1556109648429214 1106 44-45 -1.1109390746647065 1106 46-47 -1.2073812875283991 1106 48-49 -1.2928997485279226 1106 50-51 -1.306583045374282 1106 52-53 -1.317568246382372 1106 54-55 -1.4325872969976174 1106 56-57 -1.6102403350555932 1106 58-59 -1.6140510313319112 1106 60-61 -1.744923269154846 1106 62-63 -1.859435093545379 1106 64-65 -2.1422693246065876 1106 66-67 -2.145607516024871 1106 68-69 -1.8795742004406222 1106 70-71 -1.8651685249278174 1106 72-73 -1.9474301956841344 1106 74-75 -1.9788330014672333 1106 76-77 -1.8018095061848136 1106 78-79 -1.729109779403423 1106 80-81 -1.863953652941369 1106 82-83 -1.9978244701248364 1106 84-85 -1.7873011185838052 1106 86-87 -1.7052237719773267 1106 88-89 -1.9772531900282928 1106 90-91 -1.975202453580149 1106 92-93 -2.061671440108544 1106 94-95 -1.9656008785464252 1106 96-97 -2.145520626870706 1106 98-99 -2.1474472247209277 1106 100-101 -1.5301772644701686 1107 1 0.1457799122828689 1107 2 0.18598532105166043 1107 3 0.1137930722389342 1107 4 0.12256463445388732 1107 5 0.13096584200894767 1107 6 0.1322001154882173 1107 7 0.1312014218026505 1107 8 0.18025116197327407 1107 9 0.20895854501304 1107 10-11 0.20266743119137232 1107 12-13 0.20107944155635948 1107 14-15 0.2044916680594966 1107 16-17 0.23581741969528736 1107 18-19 0.28856531476927927 1107 20-21 0.22001684849011127 1107 22-23 0.1794186849105941 1107 24-25 0.23522619812487022 1107 26-27 0.2946255848844288 1107 28-29 0.3232272157285081 1107 30-31 0.2712246832759462 1107 32-33 0.30067437902056326 1107 34-35 0.3275711592480306 1107 36-37 0.27623153480062257 1107 38-39 0.37261629165668353 1107 40-41 0.33821878058044774 1107 42-43 0.40822840877984135 1107 44-45 0.37771018221560126 1107 46-47 0.31495186217387783 1107 48-49 0.28472062748596016 1107 50-51 0.3900209913831816 1107 52-53 0.3619501909564562 1107 54-55 0.26125905069800837 1107 56-57 0.29941242644284216 1107 58-59 0.2940912280332668 1107 60-61 0.404942582502521 1107 62-63 0.4209735636818621 1107 64-65 0.4355115136886951 1107 66-67 0.5144982671810965 1107 68-69 0.5778598681983169 1107 70-71 0.5593998444284622 1107 72-73 0.5289530735736818 1107 74-75 0.6732195443623823 1107 76-77 0.43975538199559594 1107 78-79 0.6787841678046043 1107 80-81 0.5184270845521866 1107 82-83 0.6848225714617335 1107 84-85 0.8150412908118518 1107 86-87 0.8493281291900985 1107 88-89 0.8322185972097458 1107 90-91 0.7950606272018845 1107 92-93 0.7662560881393823 1107 94-95 0.9355545319275755 1107 96-97 0.9554471869039673 1107 98-99 0.9567636501442784 1107 100-101 1.6060022329277395 1108 1 0.1227940226935047 1108 2 0.09359250172048661 1108 3 0.10680515307936389 1108 4 0.09846314495808173 1108 5 0.0015285602598353876 1108 6 0.05374766048183233 1108 7 0.08677939844840665 1108 8 0.08543375234384598 1108 9 0.07765556002517826 1108 10-11 0.08543097207274997 1108 12-13 0.18812554035532258 1108 14-15 0.16357264443198005 1108 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337.0 8 832.0 9 1561.0 10 2640.0 11 4327.0 12 6634.0 13 9665.0 14 12620.0 15 17328.0 16 23560.0 17 33361.0 18 30237.0 19 18414.0 20 15375.0 21 15144.0 22 15418.0 23 16601.0 24 19253.0 25 22280.0 26 27266.0 27 33088.0 28 41944.0 29 52360.0 30 66141.0 31 83960.0 32 108736.0 33 144874.0 34 201624.0 35 293540.0 36 460963.0 37 730293.0 38 605480.0 39 50673.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32496280173245 20.136946488199225 15.434295697005501 22.103795013062832 2 15.281089902286713 21.096868274001395 40.3709909259016 23.251050897810295 3 19.08064756525166 25.19530657922658 30.293929296807896 25.430116558713863 4 12.896802340268584 17.45895561977709 37.48332710204824 32.160914937906085 5 13.311892353819111 38.14342676286475 34.28272407392085 14.261956809395294 6 31.167519679071976 37.28502066428867 17.39311661934258 14.15434303729677 7 27.903452552974343 33.64117144791487 20.743958600689147 17.711417398421634 8 25.986100865980216 35.72834670533432 19.73076427553732 18.554788153148145 9 26.194575245293695 17.314078241362935 20.695392469913 35.79595404343037 10-11 24.5092862989198 27.570120903772118 27.289776229499914 20.63081656780817 12-13 25.43955910351638 24.868512009104922 28.17013559171096 21.52179329566774 14-15 22.339504410739366 26.296554646686136 26.82014048179623 24.54380046077827 16-17 21.306116301041804 29.666422046623488 27.37130922668068 21.65615242565403 18-19 21.23920366702707 28.015410431171244 30.097580661459332 20.64780524034235 20-21 22.12369693500957 26.947916872249305 30.404562947758983 20.52382324498214 22-23 21.826813510504646 26.88089787513474 30.211709708218056 21.08057890614256 24-25 21.652805215080384 26.991590291321 30.377167476733728 20.97843701686489 26-27 21.434497894159303 27.27208567041521 30.401976766676007 20.89143966874948 28-29 21.21530388991789 27.456470936336103 30.412785798497428 20.915439375248578 30-31 21.48872874205511 27.161350706829822 30.3430638218324 21.00685672928266 32-33 21.44059229216227 27.227415048334834 30.151125002388046 21.180867657114852 34-35 21.768726064286653 27.21301142851369 30.116321882925938 20.901940624273717 36-37 21.716058208843993 27.129023457087964 30.081479624252083 21.073438709815953 38-39 21.449288496710892 27.516581323322857 30.017121297858797 21.017008882107454 40-41 21.75755246678601 27.34992561922665 29.871893288299624 21.020628625687717 42-43 21.57071356863595 27.361189392131667 29.700028883919966 21.36806815531242 44-45 21.748105984054646 27.55430059719289 29.47852038135465 21.219073037397816 46-47 21.908440528278245 27.78713698554005 29.01226342168285 21.292159064498854 48-49 21.968753473520405 27.79488925153845 28.91023665410305 21.32612062083809 50-51 22.033471474187323 28.010231728019562 28.718198341804513 21.238098455988602 52-53 22.087582936233577 28.178244043582584 28.35488837287805 21.379284647305788 54-55 21.908598415569454 28.203327885169205 28.433101260066895 21.454972439194446 56-57 21.97699519012156 28.374493497569798 28.372677793720886 21.275833518587756 58-59 22.06084913048311 28.059634661439127 28.392555803684203 21.486960404393564 60-61 22.027613855683438 28.143378080696824 28.697246698260965 21.13176136535877 62-63 21.86164273516365 28.165813864777746 28.721908693147945 21.250634706910667 64-65 21.863174241888384 28.080965234481575 28.604551069591665 21.451309454038377 66-67 21.896456882875416 28.13843620848196 28.41717043238382 21.5479364762588 68-69 21.81823951881006 28.136952067944588 28.472936223639138 21.571872189606218 70-71 22.005778043309114 28.134757434596768 28.273003546780114 21.586460975314008 72-73 22.053665258733062 28.150025135656758 28.08567027575963 21.71063932985055 74-75 21.830981023210693 28.607582505582897 27.85513904186413 21.706297429342282 76-77 21.856921905156472 28.28696078332306 28.080870502106848 21.77524680941362 78-79 21.89563586896113 28.295865626547297 27.962960273031335 21.845538231460242 80-81 21.872963253943393 28.570668456898034 27.870248855632912 21.68611943352566 82-83 22.003397108321273 28.506295449902126 27.877760939948736 21.612546501827865 84-85 21.893823155218296 28.411815545670393 27.72972558198137 21.964635717129948 86-87 21.70225551468731 28.3717146812445 28.004216222224464 21.92181358184372 88-89 21.874936845083518 28.281024221173567 27.91284684680133 21.931192086941582 90-91 21.929929692835458 28.24495789860136 27.897803937359118 21.927308471204064 92-93 21.801859297014005 28.116171406223035 28.031099544341338 22.05086975242162 94-95 21.75535301072118 28.1192055364126 27.998421758637065 22.127019694229155 96-97 21.610333533744935 28.29327627497145 27.99924277255136 22.097147418732256 98-99 21.595867127909678 28.302863611747487 27.789886020768567 22.31138323957427 100-101 21.524122394929467 28.422235788392587 27.835333992552364 22.218307824125585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 597.0 1 426.5 2 313.0 3 415.5 4 411.0 5 430.0 6 775.5 7 1616.0 8 2665.5 9 3306.0 10 3465.5 11 3507.0 12 3752.0 13 4217.5 14 4652.0 15 5053.0 16 5458.5 17 5702.5 18 5959.5 19 6485.5 20 7069.0 21 7895.0 22 9154.5 23 10960.0 24 13398.0 25 16662.5 26 21151.5 27 26338.5 28 30906.0 29 36159.5 30 42700.5 31 49002.5 32 55806.5 33 63419.5 34 71704.0 35 79227.5 36 88266.5 37 95997.0 38 99101.5 39 100514.5 40 101913.0 41 106737.5 42 110857.0 43 114173.5 44 119888.0 45 123433.0 46 123369.0 47 124287.5 48 127829.0 49 124184.5 50 116996.5 51 110573.5 52 101790.5 53 94412.5 54 89810.5 55 83073.0 56 72477.5 57 63046.5 58 56074.0 59 49206.0 60 40091.0 61 31925.5 62 25590.0 63 19442.5 64 14051.5 65 10183.5 66 7356.5 67 5702.5 68 4236.5 69 2735.5 70 1953.0 71 1399.5 72 1041.0 73 826.0 74 618.5 75 441.5 76 283.0 77 180.0 78 105.5 79 68.0 80 38.5 81 20.5 82 15.5 83 14.5 84 11.5 85 4.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04250325879369057 2 0.0 3 0.0 4 0.0 5 0.00928377272313895 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005589210108828552 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1052110384689227E-4 22-23 0.0015946616412194456 24-25 0.0 26-27 8.999575598961228E-4 28-29 0.001657816557703384 30-31 0.0 32-33 1.578872912098461E-5 34-35 0.0 36-37 3.7892949890363066E-4 38-39 0.0 40-41 9.473237472590767E-5 42-43 0.021930544749047626 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.578872912098461E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5788729120984611E-4 84-85 1.5788729120984611E-4 86-87 0.0 88-89 0.0 90-91 0.01116263148853612 92-93 0.0025735628467204916 94-95 0.0 96-97 0.0 98-99 3.473520406616614E-4 100-101 0.013988814001192364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3166816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.49883687931428 #Duplication Level Percentage of deduplicated Percentage of total 1 69.60663045601567 33.06233985748998 2 15.444130172208991 14.671564395853018 3 5.9959637056121196 8.544039059614766 4 2.902759808575973 5.515108585895188 5 1.6340986176447858 3.8808891842111324 6 1.0590570178568992 3.0182385922246735 7 0.6905539747960023 2.29603574236382 8 0.4734618039407587 1.7991107995174382 9 0.33462063134786996 1.430468170636107 >10 1.6763268602958883 14.192024128441915 >50 0.11611880293778017 3.7903542130155183 >100 0.06254017065290671 5.219428998231222 >500 0.0028034835862734715 0.8641276240673142 >1k 7.342457007219902E-4 0.5642907153051336 >5k 6.674960915654456E-5 0.2638514064933881 >10k+ 1.3349921831308913E-4 0.8881285266393643 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15802 0.4989869951395976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12210 0.3855607651344442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8322 0.26278760748966784 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3236 0.10218465487101241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.157745824196922E-5 2 0.0 0.0 0.0 0.0 3.157745824196922E-5 3 0.0 0.0 0.0 0.0 3.157745824196922E-5 4 0.0 0.0 0.0 0.0 3.157745824196922E-5 5 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 6 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 7 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 8 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 9 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 10-11 0.0 0.0 0.0 3.157745824196922E-5 3.157745824196922E-5 12-13 0.0 0.0 0.0 6.315491648393844E-5 3.157745824196922E-5 14-15 0.0 0.0 0.0 6.315491648393844E-5 3.157745824196922E-5 16-17 0.0 0.0 0.0 6.315491648393844E-5 3.157745824196922E-5 18-19 0.0 0.0 0.0 6.315491648393844E-5 3.157745824196922E-5 20-21 0.0 0.0 0.0 1.5788729120984611E-4 3.157745824196922E-5 22-23 0.0 0.0 0.0 2.684083950567384E-4 3.157745824196922E-5 24-25 0.0 0.0 0.0 3.9471822802461526E-4 3.157745824196922E-5 26-27 0.0 0.0 0.0 6.315491648393845E-4 3.157745824196922E-5 28-29 0.0 0.0 0.0 0.0013578307044046765 3.157745824196922E-5 30-31 0.0 0.0 0.0 0.002684083950567384 3.157745824196922E-5 32-33 0.0 0.0 0.0 0.004926083485747199 3.157745824196922E-5 34-35 0.0 0.0 0.0 0.007199660479168982 3.157745824196922E-5 36-37 0.0 3.157745824196922E-5 0.0 0.01092580055172135 3.157745824196922E-5 38-39 0.0 3.157745824196922E-5 0.0 0.014936137748451442 3.157745824196922E-5 40-41 0.0 3.157745824196922E-5 0.0 0.01998853106716652 3.157745824196922E-5 42-43 0.0 3.157745824196922E-5 0.0 0.027898684356779807 3.157745824196922E-5 44-45 0.0 3.157745824196922E-5 0.0 0.038856062366743126 3.157745824196922E-5 46-47 0.0 3.157745824196922E-5 0.0 0.0626812546103089 3.157745824196922E-5 48-49 0.0 3.157745824196922E-5 0.0 0.10562659781938705 3.157745824196922E-5 50-51 0.0 3.157745824196922E-5 0.0 0.1396039428877459 3.157745824196922E-5 52-53 0.0 3.157745824196922E-5 0.0 0.17099193638026333 3.157745824196922E-5 54-55 0.0 3.157745824196922E-5 0.0 0.21477408223275365 3.157745824196922E-5 56-57 0.0 3.157745824196922E-5 0.0 0.2504092438588159 3.157745824196922E-5 58-59 0.0 3.157745824196922E-5 0.0 0.29834382547012517 3.157745824196922E-5 60-61 0.0 3.157745824196922E-5 0.0 0.36762476885300566 3.157745824196922E-5 62-63 0.0 3.157745824196922E-5 0.0 0.42129065913523234 3.157745824196922E-5 64-65 0.0 3.157745824196922E-5 0.0 0.4875401665268838 3.157745824196922E-5 66-67 0.0 3.157745824196922E-5 0.0 0.5919194547457131 3.157745824196922E-5 68-69 3.157745824196922E-5 3.157745824196922E-5 0.0 0.7037668118387681 3.157745824196922E-5 70-71 3.157745824196922E-5 3.157745824196922E-5 0.0 0.8055725372108768 3.157745824196922E-5 72-73 3.157745824196922E-5 3.157745824196922E-5 0.0 0.9282825399391692 3.157745824196922E-5 74-75 3.157745824196922E-5 3.157745824196922E-5 0.0 1.049713655608662 3.157745824196922E-5 76-77 3.157745824196922E-5 3.157745824196922E-5 0.0 1.1586085203560927 3.157745824196922E-5 78-79 3.157745824196922E-5 3.157745824196922E-5 0.0 1.2920864363448965 3.157745824196922E-5 80-81 3.157745824196922E-5 3.157745824196922E-5 0.0 1.4561313319119267 3.157745824196922E-5 82-83 3.157745824196922E-5 3.157745824196922E-5 0.0 1.6244076068833806 3.157745824196922E-5 84-85 3.157745824196922E-5 3.157745824196922E-5 0.0 1.827150677525944 3.157745824196922E-5 86-87 3.157745824196922E-5 3.157745824196922E-5 0.0 2.116668603417439 3.157745824196922E-5 88-89 3.157745824196922E-5 3.157745824196922E-5 0.0 2.5161234501783496 3.157745824196922E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10985 0.0 36.46584 1 GTATCAA 16735 0.0 33.959724 1 ATCAACG 20905 0.0 26.970001 3 TATCAAC 21065 0.0 26.855343 2 TCAACGC 21080 0.0 26.746105 4 CAACGCA 21400 0.0 26.368357 5 AACGCAG 21930 0.0 25.686556 6 GTACATG 13080 0.0 24.267633 1 TACATGG 13365 0.0 23.420536 2 ACGCAGA 24705 0.0 22.670303 7 CGCAGAG 24795 0.0 22.511383 8 ACCGTCG 150 3.033506E-5 22.168444 8 ACATGGG 13440 0.0 21.840855 3 GAGTACT 14465 0.0 21.70758 12-13 AGTACTT 14990 0.0 21.121607 12-13 GTACCGT 225 7.167546E-8 21.109468 6 GCAGAGT 26315 0.0 20.813942 9 AGAGTAC 21825 0.0 20.601307 10-11 GTACTTT 15165 0.0 20.498735 14-15 CATGGGG 6460 0.0 18.74943 4 >>END_MODULE