FastQCFastQC Report
Thu 26 May 2016
SRR937828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664001
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129840.48738720443423256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93200.34984971852488045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.23922663692693807No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37360.14024018759752718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127900.035.7007641
GGTATCA83750.035.3818051
ATCAACG157100.028.7444733
TATCAAC157500.028.672012
TCAACGC158400.028.4222834
CAACGCA159200.028.2789235
AACGCAG163550.027.594246
GTACATG109250.026.1655481
TACATGG110000.025.5393522
ACGCAGA184250.024.2358117
CGCAGAG185350.024.0919788
ACATGGG114250.023.46553
GAGTACT103200.022.64293712-13
GCAGAGT195700.022.258269
AGTACTT108500.021.42744312-13
GTACTTT109250.021.21533214-15
CATGGGG55300.021.1482354
AGAGTAC164000.020.92482210-11
CAGAGTA190200.018.77911410-11
CATGGGA56050.018.4055294
Produced by FastQC (version 0.11.5)