##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937828_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2664001 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.854777456915368 33.0 31.0 34.0 30.0 34.0 2 31.96364303166553 33.0 31.0 34.0 30.0 34.0 3 32.01099136224048 34.0 31.0 34.0 30.0 34.0 4 35.60455683012131 37.0 35.0 37.0 33.0 37.0 5 35.61275652674305 37.0 35.0 37.0 33.0 37.0 6 35.67386648878885 37.0 35.0 37.0 33.0 37.0 7 35.59795885962505 37.0 35.0 37.0 33.0 37.0 8 35.60807634831969 37.0 35.0 37.0 33.0 37.0 9 37.193835512824506 39.0 37.0 39.0 34.0 39.0 10-11 37.12510599658184 39.0 37.0 39.0 34.0 39.0 12-13 37.033566053466195 39.0 37.0 39.0 33.0 39.0 14-15 38.190745236206745 40.0 38.0 41.0 33.0 41.0 16-17 38.10646448706288 40.0 38.0 41.0 33.0 41.0 18-19 38.04238249159816 40.0 38.0 41.0 33.0 41.0 20-21 38.0606535057607 40.0 38.0 41.0 33.5 41.0 22-23 38.06202005930177 40.0 38.0 41.0 33.0 41.0 24-25 37.99927195973275 40.0 38.0 41.0 33.0 41.0 26-27 37.896509235544585 40.0 38.0 41.0 33.0 41.0 28-29 37.74972813448643 40.0 38.0 41.0 33.0 41.0 30-31 37.513386631611624 40.0 37.0 41.0 32.5 41.0 32-33 37.3778532740791 40.0 37.0 41.0 32.0 41.0 34-35 37.359929294320835 40.0 37.0 41.0 32.0 41.0 36-37 37.20384939795443 40.0 37.0 41.0 31.5 41.0 38-39 37.044791462165364 40.0 36.5 41.0 31.0 41.0 40-41 36.81657401780255 39.5 36.0 41.0 30.5 41.0 42-43 36.56033218456 39.0 36.0 41.0 30.0 41.0 44-45 36.29776002336335 39.0 36.0 41.0 30.0 41.0 46-47 35.915556901067234 39.0 35.0 41.0 28.5 41.0 48-49 35.546636619130396 39.0 35.0 40.0 27.0 41.0 50-51 34.69331092593434 38.0 34.0 39.5 25.0 40.5 52-53 34.80560930720371 38.0 34.5 40.0 26.0 40.5 54-55 35.08141945141912 39.0 35.0 40.0 26.0 41.0 56-57 34.857153957524794 38.5 34.5 40.0 25.5 41.0 58-59 34.590861827754566 38.0 34.0 40.0 24.5 41.0 60-61 34.25570166828015 38.0 34.0 40.0 23.5 41.0 62-63 33.90071268742016 38.0 34.0 40.0 22.5 41.0 64-65 33.48332620746014 37.0 33.0 40.0 21.5 41.0 66-67 32.983554060227455 36.5 32.5 39.0 19.0 41.0 68-69 32.532324124502956 36.0 32.0 39.0 18.0 40.0 70-71 32.06899528190868 35.5 31.0 38.5 15.0 40.0 72-73 31.599833671233608 35.0 31.0 38.0 10.5 40.0 74-75 31.06112741699421 35.0 30.5 37.0 7.5 39.0 76-77 30.510123119323154 34.5 30.0 36.5 6.0 39.0 78-79 29.978368626738504 34.0 29.5 36.0 2.0 39.0 80-81 29.42663910411445 34.0 29.0 36.0 2.0 37.5 82-83 28.870554853395326 34.0 29.0 35.0 2.0 37.0 84-85 28.315368124861816 34.0 27.5 35.0 2.0 36.5 86-87 27.73430509222782 33.0 26.0 35.0 2.0 36.0 88-89 27.191582510667224 33.0 26.0 35.0 2.0 36.0 90-91 26.617546690110103 32.5 25.0 35.0 2.0 35.0 92-93 25.945114885467387 32.0 23.5 34.0 2.0 35.0 94-95 25.177611607503152 32.0 19.5 34.0 2.0 35.0 96-97 24.29677428799764 31.0 12.5 34.0 2.0 35.0 98-99 23.113104311897782 31.0 2.0 34.0 2.0 35.0 100-101 19.877676660031284 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19263539038903588 1101 2 0.0984417716543895 1101 3 0.06758027261848909 1101 4 0.03633023321466311 1101 5 0.025926308036879675 1101 6 0.12612767247842527 1101 7 0.12849552990630997 1101 8 0.21994928086375154 1101 9 0.3643314409348264 1101 10-11 0.34444486895599624 1101 12-13 0.24137448105032178 1101 14-15 0.48486202849897353 1101 16-17 0.48876986782781984 1101 18-19 0.5628701260572342 1101 20-21 0.48691696900913684 1101 22-23 0.3724358412155482 1101 24-25 0.4256502008739744 1101 26-27 0.4399662833925291 1101 28-29 0.44739127392055167 1101 30-31 0.48805900249383427 1101 32-33 0.4912023791669924 1101 34-35 0.6010884592883841 1101 36-37 0.7076008382178358 1101 38-39 0.6729352463237959 1101 40-41 0.7640633465906816 1101 42-43 0.7395218591795754 1101 44-45 0.8745729295426159 1101 46-47 0.8605273254807102 1101 48-49 0.9560150393064077 1101 50-51 0.7272783004766836 1101 52-53 0.835015196890545 1101 54-55 0.9349903663150201 1101 56-57 1.1188393091996787 1101 58-59 0.984416113578618 1101 60-61 1.028707584672489 1101 62-63 0.9864518595666567 1101 64-65 1.003417989167417 1101 66-67 1.1436910120274888 1101 68-69 1.231944820821191 1101 70-71 1.218591303559041 1101 72-73 1.2782970849620447 1101 74-75 1.2916963991660069 1101 76-77 1.1920157992331148 1101 78-79 1.526635246338639 1101 80-81 1.6503425899146613 1101 82-83 1.5960740774331406 1101 84-85 1.8395914283659636 1101 86-87 2.0435217467737132 1101 88-89 2.1589035946129584 1101 90-91 2.158829572884745 1101 92-93 2.229489998645228 1101 94-95 2.5269782270061256 1101 96-97 2.6299834140420764 1101 98-99 3.031582350391563 1101 100-101 3.59190103014566 1102 1 0.1649837311348179 1102 2 0.20773106040885736 1102 3 0.13633988633827343 1102 4 0.1898034650434539 1102 5 0.08407683423300227 1102 6 0.14084778643903917 1102 7 0.21376051204372004 1102 8 0.1455570165914608 1102 9 0.2785040474023148 1102 10-11 0.3383864930635241 1102 12-13 0.33870921344894356 1102 14-15 0.4180050223996261 1102 16-17 0.44138259013598713 1102 18-19 0.3649547440654004 1102 20-21 0.44858145164488405 1102 22-23 0.45273473073425663 1102 24-25 0.3865668270001521 1102 26-27 0.4512531268625892 1102 28-29 0.40829891150747954 1102 30-31 0.5761685811231629 1102 32-33 0.49537948894286643 1102 34-35 0.6100307000398217 1102 36-37 0.646085936927058 1102 38-39 0.7364280279744051 1102 40-41 0.8411413763510822 1102 42-43 0.7714089783919107 1102 44-45 0.8105798528268835 1102 46-47 0.8108857321852909 1102 48-49 0.9193214619240209 1102 50-51 0.852469369705112 1102 52-53 0.8295139095710269 1102 54-55 0.9762482636757497 1102 56-57 1.0664086575376075 1102 58-59 1.1222201120022675 1102 60-61 1.1928721064761802 1102 62-63 1.2878090084783267 1102 64-65 1.2460644601765125 1102 66-67 1.3293809261030773 1102 68-69 1.3498187169381808 1102 70-71 1.4300482965622372 1102 72-73 1.4054564434273935 1102 74-75 1.442816541889954 1102 76-77 1.4410717515342029 1102 78-79 1.582080121786607 1102 80-81 1.6830966763356017 1102 82-83 1.9161959821116419 1102 84-85 2.077080285590199 1102 86-87 2.0748934561146832 1102 88-89 2.199100743910286 1102 90-91 2.2250588876949244 1102 92-93 2.3823947932425433 1102 94-95 2.574066271706581 1102 96-97 2.471270767699618 1102 98-99 2.759066712341859 1102 100-101 3.4612969521006356 1103 1 0.10073115640065211 1103 2 0.009083213822886904 1103 3 0.04183460311751119 1103 4 0.045506752009281115 1103 5 0.1043060524500774 1103 6 0.10079186120664474 1103 7 0.12007320849259884 1103 8 0.08635317789688202 1103 9 0.16074130748390303 1103 10-11 0.1520802334500715 1103 12-13 0.23971653639417667 1103 14-15 0.22057912960219284 1103 16-17 0.28717900222721227 1103 18-19 0.2683936442860926 1103 20-21 0.2517269324848499 1103 22-23 0.07306196439973434 1103 24-25 0.0852940890880376 1103 26-27 0.0519906460895001 1103 28-29 0.0610048902408451 1103 30-31 0.08681736397610251 1103 32-33 0.13430311630861524 1103 34-35 0.1320856388480891 1103 36-37 0.13150838559956668 1103 38-39 0.2684211898817068 1103 40-41 0.33099144824301163 1103 42-43 0.15893677381757954 1103 44-45 0.25217434891462887 1103 46-47 0.35857724893778453 1103 48-49 0.36631328630709703 1103 50-51 0.3210059927907807 1103 52-53 0.34349939743299984 1103 54-55 0.4280873595093553 1103 56-57 0.3852788257312838 1103 58-59 0.39629680229852227 1103 60-61 0.5216606438934193 1103 62-63 0.5793508395490221 1103 64-65 0.4626873786544081 1103 66-67 0.7704427113635575 1103 68-69 0.7455137697703904 1103 70-71 0.9370341438838388 1103 72-73 0.8920605111730033 1103 74-75 0.9229973584485016 1103 76-77 0.9405785394697368 1103 78-79 1.127665455833288 1103 80-81 1.2409349863737198 1103 82-83 1.4773565769755486 1103 84-85 1.4613158904855368 1103 86-87 1.5755996271171568 1103 88-89 1.6761513357647644 1103 90-91 1.775019388932133 1103 92-93 1.8884450005588 1103 94-95 2.048001894992879 1103 96-97 2.158981845744158 1103 98-99 2.330329168036368 1103 100-101 2.9947097705985932 1104 1 0.2279562108054236 1104 2 0.21198228289679477 1104 3 0.20660146859667705 1104 4 0.15207008319328708 1104 5 0.12620397297881425 1104 6 0.19500833174747356 1104 7 0.2581076849977961 1104 8 0.30284991361059355 1104 9 0.3088507416742772 1104 10-11 0.3309036798915983 1104 12-13 0.29966354211675394 1104 14-15 0.4818200117654854 1104 16-17 0.4274501193385163 1104 18-19 0.38901280081063305 1104 20-21 0.26370882778588367 1104 22-23 0.2603959794718733 1104 24-25 0.19619670454704874 1104 26-27 0.24366898291548722 1104 28-29 0.2413657752251268 1104 30-31 0.3215019170509734 1104 32-33 0.44948204755709753 1104 34-35 0.5407450649785019 1104 36-37 0.5094117365304953 1104 38-39 0.5971766303588595 1104 40-41 0.5978571792819452 1104 42-43 0.6091425548283027 1104 44-45 0.7253114892386421 1104 46-47 0.7109554426993654 1104 48-49 0.9089943332966968 1104 50-51 0.7255661705130265 1104 52-53 0.8645903138511457 1104 54-55 0.96387763919018 1104 56-57 1.1417341029941213 1104 58-59 1.0461350992276763 1104 60-61 1.132759217279407 1104 62-63 1.1243778404356135 1104 64-65 1.1693942779854325 1104 66-67 1.285512581195107 1104 68-69 1.126659745832292 1104 70-71 1.2515367935977117 1104 72-73 1.29372615355231 1104 74-75 1.1220981684750981 1104 76-77 1.3289862931571683 1104 78-79 1.5016811324674464 1104 80-81 1.5485823830046535 1104 82-83 1.5491958108757622 1104 84-85 1.7586782665635567 1104 86-87 1.9526012693352577 1104 88-89 2.0208710504419614 1104 90-91 1.956492297370918 1104 92-93 2.0981144411971506 1104 94-95 2.316232279050265 1104 96-97 2.2303100274157934 1104 98-99 2.484990850957299 1104 100-101 3.1492251147591475 1105 1 0.23982210310703778 1105 2 0.23627826513039807 1105 3 0.13951335187620018 1105 4 0.21721934717644586 1105 5 0.13706424510152004 1105 6 0.16430766723114232 1105 7 0.23097398638071098 1105 8 0.15203507130966187 1105 9 0.3156672426047251 1105 10-11 0.2824964863464601 1105 12-13 0.3066769799559097 1105 14-15 0.2318078204330476 1105 16-17 0.29846044876808975 1105 18-19 0.31771195775845484 1105 20-21 0.2766534882610827 1105 22-23 0.31921700412168263 1105 24-25 0.2865374687636404 1105 26-27 0.42330291851585145 1105 28-29 0.3549845327433232 1105 30-31 0.315861968411717 1105 32-33 0.33174601601069753 1105 34-35 0.5167637255743429 1105 36-37 0.5426522535240963 1105 38-39 0.5261359366543061 1105 40-41 0.4897747567501298 1105 42-43 0.41133146928265774 1105 44-45 0.4732062919798423 1105 46-47 0.6288581989543331 1105 48-49 0.6706764938542662 1105 50-51 0.542856166574289 1105 52-53 0.5552256470192276 1105 54-55 0.6447540261760238 1105 56-57 0.6908719654201292 1105 58-59 0.6927818238145775 1105 60-61 0.8255335336650163 1105 62-63 0.8769840735774892 1105 64-65 0.7712731118553364 1105 66-67 0.9069341463188749 1105 68-69 0.8574334569600595 1105 70-71 1.0526012160453782 1105 72-73 1.0218426611233475 1105 74-75 1.111902108531254 1105 76-77 1.1081141237133636 1105 78-79 1.2494918378260031 1105 80-81 1.3161398026597197 1105 82-83 1.3531768765452235 1105 84-85 1.7388481645669671 1105 86-87 1.8651736506193082 1105 88-89 1.8408360428154111 1105 90-91 1.8511293276941352 1105 92-93 1.9639684199563483 1105 94-95 1.9531750578528566 1105 96-97 2.0637008957276066 1105 98-99 2.4346861690294155 1105 100-101 3.187771877516834 1106 1 0.03528034070828312 1106 2 0.06561482190212331 1106 3 0.11979391560537422 1106 4 0.0777836504361602 1106 5 0.06828692252193491 1106 6 0.1072846267922003 1106 7 0.13578621101736132 1106 8 0.14181540357176914 1106 9 0.14589012278784708 1106 10-11 -0.14729585899507214 1106 12-13 -0.2712273362024433 1106 14-15 -0.36510801838770846 1106 16-17 -0.1839476728553322 1106 18-19 4.4901911616079815E-4 1106 20-21 0.07319287247319295 1106 22-23 0.17062156335098422 1106 24-25 -0.18572091382515765 1106 26-27 -0.056395084149379215 1106 28-29 0.2850314002427794 1106 30-31 0.38172778347503566 1106 32-33 0.4832470860114668 1106 34-35 0.425409585245454 1106 36-37 0.26738974605790844 1106 38-39 0.02290142876304202 1106 40-41 0.16976536065318726 1106 42-43 0.2487084527843635 1106 44-45 0.242102004770139 1106 46-47 0.34644746645575 1106 48-49 0.2725487203719581 1106 50-51 0.37498674147984445 1106 52-53 0.4954099625912818 1106 54-55 0.551548225696969 1106 56-57 0.3870916777413811 1106 58-59 0.18953456691301085 1106 60-61 0.549032463664247 1106 62-63 0.4676616766047559 1106 64-65 0.6930500704392273 1106 66-67 0.77965627224917 1106 68-69 0.5680976452801971 1106 70-71 0.4090365958162643 1106 72-73 0.42562710177312013 1106 74-75 0.7891619049623912 1106 76-77 1.0530396969760467 1106 78-79 0.9701397226812212 1106 80-81 0.855118565709514 1106 82-83 0.949473128733171 1106 84-85 0.9126802330763297 1106 86-87 1.1837231705236455 1106 88-89 1.2365611240731766 1106 90-91 1.5112260552751131 1106 92-93 1.7605500209511113 1106 94-95 1.703238955587171 1106 96-97 1.670058025584904 1106 98-99 1.7482234193473012 1106 100-101 2.4285883149761425 1107 1 0.16032022747078756 1107 2 0.1381684338018765 1107 3 0.16804973907270337 1107 4 0.16533038953991053 1107 5 0.14047275801665648 1107 6 0.16221749430268773 1107 7 0.24175674799545277 1107 8 0.168547716349444 1107 9 0.2676973408600247 1107 10-11 0.20303571250246932 1107 12-13 0.28922493877485067 1107 14-15 0.40653249347195697 1107 16-17 0.35314425488213175 1107 18-19 0.4905815454414153 1107 20-21 0.33485105723686104 1107 22-23 0.34979167323390215 1107 24-25 0.4411035476012728 1107 26-27 0.4084846214658526 1107 28-29 0.33974949287824785 1107 30-31 0.4197813912838413 1107 32-33 0.3393999770858329 1107 34-35 0.3808034005472436 1107 36-37 0.3982376568097479 1107 38-39 0.4974363900217469 1107 40-41 0.44257353191672166 1107 42-43 0.4943372727089326 1107 44-45 0.5209207020480164 1107 46-47 0.6657827279901838 1107 48-49 0.6736252538645644 1107 50-51 0.5127520395873688 1107 52-53 0.5044079048434256 1107 54-55 0.6043447991172499 1107 56-57 0.7023297505766521 1107 58-59 0.5578157951798417 1107 60-61 0.7750857810155978 1107 62-63 0.9471008687239504 1107 64-65 0.9978242090896998 1107 66-67 1.072902522893493 1107 68-69 0.9869739022245838 1107 70-71 1.1295088346295508 1107 72-73 1.2118159522979859 1107 74-75 1.1692987063304017 1107 76-77 1.2678191743162728 1107 78-79 1.3583793664412944 1107 80-81 1.467112924289463 1107 82-83 1.570511043657774 1107 84-85 1.66548498839661 1107 86-87 1.7301556575757893 1107 88-89 1.6811767179563866 1107 90-91 1.855882456384581 1107 92-93 2.041792094735481 1107 94-95 2.1208943707776875 1107 96-97 2.4263562476580347 1107 98-99 2.641417101284123 1107 100-101 3.398978427968185 1108 1 -0.012459934859609945 1108 2 -0.022293536539258696 1108 3 0.10878897298714563 1108 4 0.010442169681901703 1108 5 0.10188572410628183 1108 6 0.1619428752095402 1108 7 0.14430968845396563 1108 8 0.11589204800794306 1108 9 0.2703713171410769 1108 10-11 0.19892354585953598 1108 12-13 0.16635221963898772 1108 14-15 0.3970352498339693 1108 16-17 0.29486550633150443 1108 18-19 0.3811275288829066 1108 20-21 0.3003070266422654 1108 22-23 0.3457578238879009 1108 24-25 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-1.6710728585049033 2316 82-83 -1.7080981650081846 2316 84-85 -1.940302164420821 2316 86-87 -2.130703639375003 2316 88-89 -1.8972683925079608 2316 90-91 -1.959146050755006 2316 92-93 -2.2189809522005426 2316 94-95 -2.207926655846922 2316 96-97 -2.228366826780846 2316 98-99 -2.3199267448363976 2316 100-101 -2.270829340834517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9954.0 3 1726.0 4 1630.0 5 1924.0 6 2144.0 7 2654.0 8 3052.0 9 3978.0 10 5116.0 11 6901.0 12 9441.0 13 12164.0 14 14185.0 15 17678.0 16 22640.0 17 30248.0 18 25932.0 19 17815.0 20 17504.0 21 18485.0 22 20111.0 23 22378.0 24 25346.0 25 29017.0 26 33503.0 27 39446.0 28 46651.0 29 55934.0 30 68499.0 31 85342.0 32 108947.0 33 141288.0 34 191704.0 35 269073.0 36 389700.0 37 521370.0 38 362028.0 39 28492.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01857098839605 19.16113012498936 15.24744367342603 22.572855213188557 2 14.654040104581364 20.06256811623665 41.95391718947937 23.329474589702613 3 18.666488966595022 24.82451176717074 30.54005385968697 25.968945406547267 4 12.149603077051653 16.630331787453944 38.59731680312795 32.622748332366456 5 13.084235994956916 37.515411410787365 34.974857989143594 14.425494605112126 6 30.755471661489953 36.661063001174774 18.244764948865456 14.338700388469816 7 27.590846293912037 33.14316084282221 21.49246287292107 17.773529990344684 8 25.529256963848024 35.07927945820939 20.493190753689 18.89827282425359 9 25.84534802318587 17.108665059399783 21.305978950045198 35.740007967369145 10-11 24.056351273828444 26.994780547901403 28.164558509587806 20.784309668682347 12-13 25.016190110931653 24.36461690990549 28.874486811572314 21.74470616759055 14-15 22.08493980469747 25.688382266173658 27.441286328164598 24.785391600964267 16-17 21.00923939372967 29.10580166522732 28.050290075738655 21.834668865304355 18-19 20.889177072851222 27.471311727434472 30.805152182633076 20.83435901708123 20-21 21.74987071356708 26.424435605953754 31.005613381851006 20.820080298628156 22-23 21.380387920496084 26.40384775967375 30.857850752241983 21.357913567588184 24-25 21.20138159617011 26.447776025853585 31.053585560266363 21.297256817709947 26-27 20.975135161160853 26.707687400342977 30.985336429322928 21.331841009173242 28-29 20.818939622214224 26.88474385119094 31.106272140734664 21.190044385860173 30-31 21.08575952587367 26.584585890912045 30.982759162281013 21.34689542093327 32-33 20.89929067022184 26.64068248539283 30.868296991593073 21.591729852792252 34-35 21.340464702656057 26.66632685074909 30.757773195069632 21.23543525152522 36-37 21.26890232251639 26.51338941153991 30.741145566125596 21.476562699818107 38-39 21.027712342540944 26.868743548802314 30.68394119309618 21.419602915560557 40-41 21.30624733691182 26.712114197733992 30.626444836984216 21.35519362836997 42-43 21.04476685691719 26.6380381942812 30.454777105998065 21.86241784280355 44-45 21.234135175227703 26.823251258504115 30.278401722337733 21.664211843930445 46-47 21.32252363469628 27.059942897197086 29.86646610534322 21.75106736276341 48-49 21.378142484961536 26.99761291804264 29.82731415379158 21.796930443204246 50-51 21.458498219976587 27.21919867348382 29.67256145820481 21.649741648334782 52-53 21.44304116065713 27.45860956583521 29.314019145515356 21.7843301279923 54-55 21.366981408159106 27.279386927407334 29.449481996841804 21.904149667591756 56-57 21.456828049456703 27.51814519882016 29.34728427938821 21.67774247233493 58-59 21.585916583366345 27.256511290335013 29.37368998217182 21.783882144126824 60-61 21.534647973341666 27.25815313348609 29.65224353369578 21.55495535947646 62-63 21.3490616057428 27.287662681953723 29.72643485389177 21.636840858411706 64-65 21.256680319068323 27.25000441876006 29.588431046847507 21.90488421532411 66-67 21.409256386877612 27.27606229841663 29.32047236203015 21.99420895267561 68-69 21.289700876115123 27.24608032731864 29.501773571388952 21.962445225177287 70-71 21.376850631212918 27.160949281653206 29.39444588288673 22.067754204247148 72-73 21.43025447026216 27.205682550505824 29.210401460917794 22.153661518314223 74-75 21.154237463445824 27.535548054048466 29.1537861192865 22.156428363219213 76-77 21.300614733750777 27.28127378971472 29.126941917740623 22.291169558793882 78-79 21.365688886615526 27.248165722134548 29.13406438665088 22.25208100459905 80-81 21.290312146937275 27.541111356828974 28.974630633395464 22.193945862838284 82-83 21.455973997818486 27.399708517139707 29.10620509510235 22.03811238993946 84-85 21.394761250180736 27.26418941671983 28.959761469225032 22.38128786387441 86-87 21.210050586122485 27.25507624020426 29.1742952625268 22.360577911146454 88-89 21.396083791053034 27.06076350071749 29.10540675577917 22.437745952450307 90-91 21.397493967711462 26.96850423313216 29.227585008634748 22.406416790521636 92-93 21.413028006605895 26.793243907194825 29.309639712531173 22.484088373668108 94-95 21.199181679550076 26.912513796935432 29.29777124257974 22.59053328093475 96-97 21.066867701086288 26.980239492469575 29.312206165335514 22.64068664110862 98-99 21.066506401366418 27.10214223833734 29.09509089107269 22.736260469223552 100-101 21.30744145514436 26.50163524188325 29.625130551432328 22.56579275154006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3511.0 1 2033.0 2 524.5 3 521.0 4 448.0 5 409.0 6 716.5 7 1523.5 8 2691.0 9 3282.5 10 3033.0 11 2866.0 12 3098.0 13 3372.5 14 3681.0 15 4011.0 16 4237.5 17 4488.0 18 4808.0 19 5032.5 20 5450.5 21 6393.5 22 7619.5 23 9218.0 24 11310.5 25 14088.5 26 18068.5 27 22694.0 28 26576.0 29 31116.0 30 36735.5 31 42294.0 32 48126.5 33 54573.5 34 61546.0 35 68231.0 36 75790.5 37 81578.0 38 83903.0 39 85369.5 40 86820.0 41 90554.0 42 94145.0 43 96406.0 44 100740.0 45 104408.5 46 104949.5 47 105228.0 48 106575.0 49 103296.0 50 96792.0 51 90520.0 52 83667.5 53 78038.0 54 74242.0 55 68441.5 56 59988.5 57 52510.0 58 46230.5 59 39974.5 60 32742.5 61 26438.5 62 20776.5 63 15497.0 64 11571.5 65 8539.0 66 6016.0 67 4371.0 68 3242.0 69 2210.5 70 1567.5 71 1135.5 72 868.5 73 679.5 74 494.5 75 350.0 76 245.5 77 162.5 78 102.5 79 68.0 80 43.5 81 30.5 82 21.5 83 16.0 84 15.5 85 13.0 86 10.0 87 7.0 88 3.0 89 1.0 90 2.0 91 5.0 92 4.0 93 1.0 94 2.0 95 2.0 96 0.5 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34590827856295847 2 0.2888887804471545 3 0.2942941838235046 4 0.288738630353367 5 0.28866355530647325 6 0.21103595681833454 7 0.20127620072214686 8 0.2085960177942876 9 0.21238730766242206 10-11 0.2130629830844658 12-13 0.21555922839368302 14-15 0.2106793503455892 16-17 0.202139563761425 18-19 0.19540157830271082 20-21 0.194294221361028 22-23 0.20326568946483128 24-25 0.200337762635975 26-27 0.2067566791453907 28-29 0.2147897091630221 30-31 0.21796162989428305 32-33 0.2146020215457877 34-35 0.21392634612374395 36-37 0.20018761254218748 38-39 0.19127245072355453 40-41 0.18633626639029038 42-43 0.1961710975333718 44-45 0.19853596151052497 46-47 0.20504872182855785 48-49 0.215258928206108 50-51 0.21054796901352513 52-53 0.2248309966850613 54-55 0.20731974199709383 56-57 0.181174856916345 58-59 0.16694813552997914 60-61 0.18280773918628407 62-63 0.17533777202035583 64-65 0.18333326451454035 66-67 0.1838024835576263 68-69 0.18087455672877 70-71 0.18297665804179503 72-73 0.19236103890351391 74-75 0.19804797370571556 76-77 0.19853596151052497 78-79 0.19478220916583738 80-81 0.19237980766523738 82-83 0.18166284472115438 84-85 0.17723341695442307 86-87 0.18290158299490128 88-89 0.19059677530151076 90-91 0.18682425419509976 92-93 0.18245113271353877 94-95 0.18378371479590286 96-97 0.19093461301253264 98-99 0.1905592377780639 100-101 0.17299167680492614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2664001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.13895366938864 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28262099670255 38.20879180918442 2 14.112546792901504 14.716268467843419 3 5.2792762503242034 8.257678194706724 4 2.44718154035778 5.103739398131896 5 1.3616892491595969 3.549852633701842 6 0.8090411729051854 2.5309536138438786 7 0.5502404892145198 2.0082274361924317 8 0.38245959663722556 1.59528345515851 9 0.26002825202887686 1.2201840886739153 >10 1.3631226051213405 12.781814644546863 >50 0.10290152921162694 3.710689553320133 >100 0.046518598513287386 4.15981857049631 >500 0.001653858158001574 0.5831051904637461 >1k 5.033481350381437E-4 0.49844939928926685 >5k 1.4381375286804106E-4 0.5883539994769813 >10k+ 7.190687643402053E-5 0.48678954496967597 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12984 0.48738720443423256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9320 0.34984971852488045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6373 0.23922663692693807 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3736 0.14024018759752718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.507504689375117E-5 0.0 4 0.0 0.0 0.0 1.1261257034062675E-4 0.0 5 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 6 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 7 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 8 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 9 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 10-11 0.0 0.0 0.0 1.1261257034062675E-4 3.753752344687558E-5 12-13 0.0 0.0 0.0 1.5015009378750233E-4 3.753752344687558E-5 14-15 0.0 0.0 0.0 1.8768761723437791E-4 3.753752344687558E-5 16-17 0.0 0.0 0.0 2.252251406812535E-4 3.753752344687558E-5 18-19 0.0 0.0 0.0 2.252251406812535E-4 3.753752344687558E-5 20-21 0.0 0.0 0.0 2.815314258515669E-4 7.507504689375117E-5 22-23 0.0 0.0 0.0 4.129127579156314E-4 7.507504689375117E-5 24-25 0.0 0.0 0.0 5.818316134265716E-4 7.507504689375117E-5 26-27 0.0 0.0 0.0 8.633630392781385E-4 7.507504689375117E-5 28-29 0.0 0.0 0.0 0.0017642636020031525 7.507504689375117E-5 30-31 0.0 0.0 0.0 0.003528527204006305 7.507504689375117E-5 32-33 0.0 0.0 0.0 0.006137385083564158 7.507504689375117E-5 34-35 0.0 0.0 0.0 0.00850224906071732 7.507504689375117E-5 36-37 0.0 0.0 0.0 0.011411407127850177 7.507504689375117E-5 38-39 0.0 0.0 0.0 0.014583327859111164 7.507504689375117E-5 40-41 0.0 0.0 0.0 0.018806299246884664 7.507504689375117E-5 42-43 0.0 0.0 0.0 0.026182422604195718 7.507504689375117E-5 44-45 0.0 0.0 0.0 0.03725599202102402 7.507504689375117E-5 46-47 0.0 0.0 0.0 0.061036013124619704 7.507504689375117E-5 48-49 0.0 0.0 0.0 0.10730101077289385 7.507504689375117E-5 50-51 0.0 0.0 0.0 0.14112231939852876 7.507504689375117E-5 52-53 0.0 0.0 0.0 0.17314182689871363 7.507504689375117E-5 54-55 0.0 0.0 0.0 0.21535277201472522 7.507504689375117E-5 56-57 0.0 0.0 0.0 0.25086326919546953 7.507504689375117E-5 58-59 0.0 0.0 0.0 0.29602090990206087 7.507504689375117E-5 60-61 0.0 0.0 0.0 0.3631192330633509 7.507504689375117E-5 62-63 0.0 0.0 0.0 0.4154277719865721 7.507504689375117E-5 64-65 0.0 0.0 0.0 0.48233840753062784 7.507504689375117E-5 66-67 0.0 0.0 0.0 0.5969029290904921 7.507504689375117E-5 68-69 0.0 0.0 0.0 0.7162347161281095 7.507504689375117E-5 70-71 0.0 0.0 0.0 0.8190875303725487 7.507504689375117E-5 72-73 0.0 0.0 0.0 0.9478787733187788 7.507504689375117E-5 74-75 0.0 0.0 0.0 1.072522119924129 7.507504689375117E-5 76-77 0.0 0.0 0.0 1.1830138201900073 7.507504689375117E-5 78-79 0.0 0.0 0.0 1.3202322371500612 7.507504689375117E-5 80-81 0.0 0.0 0.0 1.490558749790259 7.507504689375117E-5 82-83 0.0 0.0 0.0 1.668899523686365 7.507504689375117E-5 84-85 0.0 0.0 0.0 1.8706261746898742 7.507504689375117E-5 86-87 0.0 0.0 0.0 2.1883437731442292 7.507504689375117E-5 88-89 0.0 0.0 0.0 2.632375137997321 7.507504689375117E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12790 0.0 35.700764 1 GGTATCA 8375 0.0 35.381805 1 ATCAACG 15710 0.0 28.744473 3 TATCAAC 15750 0.0 28.67201 2 TCAACGC 15840 0.0 28.422283 4 CAACGCA 15920 0.0 28.278923 5 AACGCAG 16355 0.0 27.59424 6 GTACATG 10925 0.0 26.165548 1 TACATGG 11000 0.0 25.539352 2 ACGCAGA 18425 0.0 24.235811 7 CGCAGAG 18535 0.0 24.091978 8 ACATGGG 11425 0.0 23.4655 3 GAGTACT 10320 0.0 22.642937 12-13 GCAGAGT 19570 0.0 22.25826 9 AGTACTT 10850 0.0 21.427443 12-13 GTACTTT 10925 0.0 21.215332 14-15 CATGGGG 5530 0.0 21.148235 4 AGAGTAC 16400 0.0 20.924822 10-11 CAGAGTA 19020 0.0 18.779114 10-11 CATGGGA 5605 0.0 18.405529 4 >>END_MODULE