FastQCFastQC Report
Thu 26 May 2016
SRR937828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664001
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129480.486035853590145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.3746244839998183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.2643392401128979No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29540.11088584426207047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152700.032.1540761
GGTATCA105200.031.7624721
ATCAACG187900.025.8644583
TATCAAC189450.025.753152
TCAACGC189300.025.6480774
CAACGCA191750.025.2951225
AACGCAG196800.024.7142756
GTACATG114800.024.0551991
TACATGG116250.023.4161872
ACATGGG118800.022.113793
ACGCAGA220300.021.7992887
GAGTACT116500.021.77313412-13
CGCAGAG221250.021.576878
AGTACTT122550.021.55098312-13
AGAGTAC188500.020.48730510-11
GTACTTT124800.020.36161414-15
GCAGAGT232600.020.1564049
ATACGTC4450.019.2158185
CATGGGG55950.018.764924
TACTTTT144350.018.0646114-15
Produced by FastQC (version 0.11.5)