##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937828_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2664001 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.307987121626454 33.0 31.0 34.0 31.0 34.0 2 32.538565113151236 34.0 31.0 34.0 31.0 34.0 3 32.6455151480799 34.0 31.0 34.0 31.0 34.0 4 36.233960122387344 37.0 35.0 37.0 35.0 37.0 5 36.08939260908686 37.0 35.0 37.0 35.0 37.0 6 36.177761194534085 37.0 36.0 37.0 35.0 37.0 7 36.141431253216496 37.0 36.0 37.0 35.0 37.0 8 36.13656526405208 37.0 36.0 37.0 35.0 37.0 9 37.818667860860415 39.0 38.0 39.0 35.0 39.0 10-11 37.77381765247085 39.0 37.5 39.0 35.0 39.0 12-13 37.702794968920806 39.0 37.0 39.0 35.0 39.0 14-15 38.96776727936664 40.0 38.0 41.0 36.0 41.0 16-17 38.946870140063766 40.0 38.0 41.0 36.0 41.0 18-19 38.9279987132137 40.0 38.0 41.0 35.5 41.0 20-21 38.85158695511 40.0 38.0 41.0 35.0 41.0 22-23 38.7070856955384 40.0 38.0 41.0 34.5 41.0 24-25 38.656106360320436 40.0 38.0 41.0 34.5 41.0 26-27 38.591496399588436 40.0 38.0 41.0 34.0 41.0 28-29 38.483373504739674 40.0 38.0 41.0 34.0 41.0 30-31 38.55635958845361 40.0 38.0 41.0 35.0 41.0 32-33 38.48601689714081 40.0 38.0 41.0 34.0 41.0 34-35 38.36485402970945 40.0 38.0 41.0 34.0 41.0 36-37 38.219235841127684 40.0 38.0 41.0 34.0 41.0 38-39 38.09431565528692 40.0 38.0 41.0 34.0 41.0 40-41 37.92128193645573 40.0 38.0 41.0 33.0 41.0 42-43 37.72351643261395 40.0 37.0 41.0 33.0 41.0 44-45 37.433229942481255 40.0 37.0 41.0 33.0 41.0 46-47 37.084197228154196 40.0 37.0 41.0 32.0 41.0 48-49 36.76270410559155 40.0 37.0 41.0 31.0 41.0 50-51 36.52422465306883 40.0 36.0 41.0 31.0 41.0 52-53 36.315522216395564 39.0 36.0 41.0 30.0 41.0 54-55 36.11674451323404 39.0 35.5 40.5 30.0 41.0 56-57 35.84792798501202 39.0 35.0 40.0 29.0 41.0 58-59 35.607338172921104 38.5 35.0 40.0 28.5 41.0 60-61 35.28547718262868 38.0 35.0 40.0 28.0 41.0 62-63 34.93452986691822 38.0 34.0 40.0 27.5 41.0 64-65 34.55521075254852 37.5 34.0 40.0 26.5 41.0 66-67 34.14392149252197 37.0 34.0 39.5 26.0 41.0 68-69 33.70903107769104 36.0 33.0 39.0 26.0 40.0 70-71 33.30239421832049 36.0 33.0 39.0 25.5 40.0 72-73 32.858862290216855 35.0 32.5 38.0 24.5 40.0 74-75 32.372296969858496 35.0 32.0 37.5 23.5 39.0 76-77 31.136098484948015 34.0 30.5 36.0 21.5 38.5 78-79 31.682672228726638 35.0 31.5 36.0 22.5 39.0 80-81 31.477094790880333 35.0 31.5 36.0 21.5 37.5 82-83 31.12150051745476 34.0 31.0 35.5 20.5 37.0 84-85 30.731644620253523 34.0 31.0 35.0 19.0 37.0 86-87 30.32414327171799 34.0 31.0 35.0 18.0 36.0 88-89 29.910405063661763 34.0 30.0 35.0 13.5 36.0 90-91 29.425132910986143 34.0 29.5 35.0 7.0 35.5 92-93 28.98982995877254 34.0 29.0 35.0 2.0 35.0 94-95 28.55754596188215 34.0 29.0 35.0 2.0 35.0 96-97 28.055181660967847 33.5 29.0 35.0 2.0 35.0 98-99 27.302912799206908 33.0 27.0 35.0 2.0 35.0 100-101 24.90735213687983 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.223134826254757 1101 2 0.20193345496202397 1101 3 0.11657075068004019 1101 4 0.11059840557120282 1101 5 -0.04511316496689233 1101 6 0.0776141816989977 1101 7 0.07146974696225783 1101 8 0.10218954152516346 1101 9 0.2305192261104665 1101 10-11 0.17765733180319643 1101 12-13 0.21481580926745636 1101 14-15 0.283554457365085 1101 16-17 0.08809874814112817 1101 18-19 0.26091063582995844 1101 20-21 0.36311633470411664 1101 22-23 0.3105165806503294 1101 24-25 0.2979129118641808 1101 26-27 0.23479314619429914 1101 28-29 0.2339050788427386 1101 30-31 0.2502670099003339 1101 32-33 0.25573021031561183 1101 34-35 0.14981263596056493 1101 36-37 0.25069233279266 1101 38-39 0.29136205136556015 1101 40-41 0.1580738369482475 1101 42-43 0.09690481775216853 1101 44-45 0.1380517312745937 1101 46-47 0.09375124474818364 1101 48-49 0.052750282620081634 1101 50-51 0.1755879341810953 1101 52-53 0.19422389894877057 1101 54-55 0.1873667032041837 1101 56-57 0.13828821316243278 1101 58-59 0.09814640551824283 1101 60-61 0.10385702373737615 1101 62-63 0.15923853224206397 1101 64-65 0.06939759055721595 1101 66-67 0.2324626763221005 1101 68-69 0.18524654943402652 1101 70-71 0.1073447918798891 1101 72-73 0.09191998268504875 1101 74-75 0.1376765998399918 1101 76-77 0.029905098486480597 1101 78-79 0.08123456110797633 1101 80-81 0.16671464874411512 1101 82-83 0.12533028241015387 1101 84-85 0.20436531732751817 1101 86-87 0.44191143011668643 1101 88-89 0.3827491011883062 1101 90-91 0.40977622894622456 1101 92-93 0.5868429541855136 1101 94-95 0.545136138360867 1101 96-97 0.7326915943501255 1101 98-99 1.0054476563691033 1101 100-101 1.583448366221159 1102 1 0.23057201394067306 1102 2 0.24490268985353936 1102 3 0.18925579507639156 1102 4 0.14549553048397712 1102 5 0.11313674735718848 1102 6 0.09459398430063004 1102 7 0.11462718880991929 1102 8 0.17276534232868812 1102 9 0.236612049700895 1102 10-11 0.14884017536324734 1102 12-13 0.1844192811133425 1102 14-15 0.2926204788387565 1102 16-17 0.2544000817390568 1102 18-19 0.2776922386301308 1102 20-21 0.30009407044387615 1102 22-23 0.3504259962606966 1102 24-25 0.22716361718833156 1102 26-27 0.18644841331761342 1102 28-29 0.16412463822554457 1102 30-31 0.2592774932487387 1102 32-33 0.25776774645556344 1102 34-35 0.24225368620760435 1102 36-37 0.30651628356398675 1102 38-39 0.26863063815829236 1102 40-41 0.21806610981740704 1102 42-43 0.2910347693325619 1102 44-45 0.30634264539799005 1102 46-47 0.35241504164375925 1102 48-49 0.35397516442330357 1102 50-51 0.31110809040290377 1102 52-53 0.1139275201952259 1102 54-55 0.18536436799172407 1102 56-57 0.28186521391084085 1102 58-59 0.29202909694600976 1102 60-61 0.27256668327360245 1102 62-63 0.30972390331909594 1102 64-65 0.2871633831696201 1102 66-67 0.2756318183353983 1102 68-69 0.1948271271189057 1102 70-71 0.2251329704744336 1102 72-73 0.3098644413460008 1102 74-75 0.159299359584665 1102 76-77 0.13017521664509957 1102 78-79 0.34912064950056987 1102 80-81 0.2899828735849397 1102 82-83 0.29073421832578816 1102 84-85 0.37269650412402555 1102 86-87 0.5019065652581212 1102 88-89 0.43424569799677926 1102 90-91 0.5654044002936907 1102 92-93 0.7928077990543727 1102 94-95 0.8101086344350392 1102 96-97 1.0209066452021602 1102 98-99 1.2992436360114148 1102 100-101 2.080050196227493 1103 1 0.1756942302183475 1103 2 0.2329172187106252 1103 3 0.1723808368740265 1103 4 0.07106565019239497 1103 5 -0.040705291029759394 1103 6 0.01686083710608699 1103 7 0.07260426698821476 1103 8 0.07522095324288358 1103 9 0.1757421573715945 1103 10-11 0.1569420194747977 1103 12-13 0.22539350033357408 1103 14-15 0.19603032970480427 1103 16-17 0.10442641424759103 1103 18-19 0.1306782615017923 1103 20-21 0.16965201868168833 1103 22-23 0.17910397893975727 1103 24-25 0.20153330888899035 1103 26-27 0.14938143679116678 1103 28-29 0.16078521637040666 1103 30-31 0.16052786942743325 1103 32-33 0.10931446784926635 1103 34-35 0.24288378551877798 1103 36-37 0.16602691688171234 1103 38-39 0.1653819171658455 1103 40-41 0.18682927607166988 1103 42-43 0.23696827765865436 1103 44-45 0.1417669749639643 1103 46-47 0.19902880644011844 1103 48-49 0.12406561450021059 1103 50-51 0.07801000807828729 1103 52-53 0.021415148095975667 1103 54-55 -0.088681810032746 1103 56-57 0.012182708727934255 1103 58-59 -0.03928443545552085 1103 60-61 -0.115944401458556 1103 62-63 -0.015607133930956252 1103 64-65 -0.020102717278447813 1103 66-67 0.07379523035974245 1103 68-69 -0.14392805578753354 1103 70-71 -0.0018864821437674095 1103 72-73 0.0941999425832094 1103 74-75 0.13914412317726033 1103 76-77 0.1680737570717703 1103 78-79 0.1972228279931798 1103 80-81 0.15625302004384523 1103 82-83 0.057787861407444296 1103 84-85 0.11470542373882253 1103 86-87 0.25246748948737263 1103 88-89 0.3500558615091194 1103 90-91 0.5098497039116943 1103 92-93 0.632301546128538 1103 94-95 0.6190815412095958 1103 96-97 0.8235363432560625 1103 98-99 1.1588134129792458 1103 100-101 1.8644289580144253 1104 1 0.1544048371337965 1104 2 0.195557764370335 1104 3 0.12437315975626717 1104 4 0.10783521576021826 1104 5 0.0020044695892451614 1104 6 0.032445285401401236 1104 7 0.054487981826611076 1104 8 0.03892037695530348 1104 9 0.1186584546231515 1104 10-11 0.1071432667545622 1104 12-13 0.1184851018488402 1104 14-15 0.09330303865942113 1104 16-17 0.10904427110259007 1104 18-19 0.11211011448801145 1104 20-21 0.1233857979998163 1104 22-23 0.19565688254256486 1104 24-25 0.14241144220083157 1104 26-27 0.10223164232029802 1104 28-29 0.04133173245003974 1104 30-31 0.1814210648010075 1104 32-33 0.12776196724477273 1104 34-35 0.12419851712190422 1104 36-37 -0.0021069994558402527 1104 38-39 -0.18309500110609633 1104 40-41 -0.07206968217770537 1104 42-43 0.07025140411952435 1104 44-45 -0.03006545833981278 1104 46-47 0.1317112135490035 1104 48-49 0.09163533320739248 1104 50-51 -0.036938458513148476 1104 52-53 5.811430988060806E-4 1104 54-55 0.014277205039384455 1104 56-57 0.16751739882223404 1104 58-59 0.17244029655061155 1104 60-61 0.12386729758230786 1104 62-63 0.04669640439418998 1104 64-65 0.020398612615970535 1104 66-67 0.1002127769060408 1104 68-69 0.03238722993827281 1104 70-71 0.0750259966988196 1104 72-73 0.15136676373256108 1104 74-75 0.14607922029985332 1104 76-77 -0.0594432192319303 1104 78-79 0.02779604271067626 1104 80-81 0.15491405816748482 1104 82-83 0.16845346348691592 1104 84-85 0.24060889556923826 1104 86-87 0.36675680197226157 1104 88-89 0.3533289633870922 1104 90-91 0.3645882083849763 1104 92-93 0.6275884809234107 1104 94-95 0.8384876728288475 1104 96-97 1.0993541779461573 1104 98-99 1.1701293535772983 1104 100-101 1.749495100111627 1105 1 0.1619529193941176 1105 2 0.2228522567774931 1105 3 0.140191578350354 1105 4 0.044716759096729675 1105 5 -0.0015460722280380423 1105 6 0.0500348688836425 1105 7 0.10194678602164231 1105 8 0.1207377523160389 1105 9 0.1715010120554794 1105 10-11 0.1159177311596693 1105 12-13 0.10575182539150063 1105 14-15 0.21860149819205787 1105 16-17 0.22701206469446333 1105 18-19 0.23113340880401267 1105 20-21 0.2216629422169376 1105 22-23 0.2150326863214218 1105 24-25 0.14844658263940147 1105 26-27 0.14601748445749507 1105 28-29 0.04185005417245691 1105 30-31 0.1682043424496058 1105 32-33 0.2193473625861131 1105 34-35 0.16778965278127345 1105 36-37 0.2468900416748241 1105 38-39 0.24395378906257292 1105 40-41 0.19675872823982132 1105 42-43 0.18032519589764462 1105 44-45 0.3439930590420843 1105 46-47 0.33913038704256593 1105 48-49 0.27620480682063686 1105 50-51 0.38000541522823994 1105 52-53 0.21844841522607084 1105 54-55 0.2219562237309063 1105 56-57 0.22248145086629734 1105 58-59 0.1930413672588145 1105 60-61 0.32092459887245894 1105 62-63 0.26943093293431986 1105 64-65 0.342227636908909 1105 66-67 0.32278149318661775 1105 68-69 0.30386447979670805 1105 70-71 0.30914773665795536 1105 72-73 0.4166945635696351 1105 74-75 0.4977939907707736 1105 76-77 0.32405377196749896 1105 78-79 0.5225755671524013 1105 80-81 0.464019488100778 1105 82-83 0.5509223781237615 1105 84-85 0.6070186064597749 1105 86-87 0.7899261124598986 1105 88-89 0.7204474950741648 1105 90-91 0.6945557201314898 1105 92-93 0.9315319334175136 1105 94-95 0.9867878056915558 1105 96-97 1.0868731936367304 1105 98-99 1.2863591345946084 1105 100-101 1.9176636071372322 1106 1 -0.08789821491662764 1106 2 0.008692854756461088 1106 3 -0.04476205494962926 1106 4 0.05761158144117928 1106 5 -0.0767899676659951 1106 6 -0.10734410560123564 1106 7 -0.19527905987137473 1106 8 -0.11623733962952087 1106 9 -0.04763859617278143 1106 10-11 -0.05351814269261013 1106 12-13 -0.1647914870426277 1106 14-15 -0.01435032673334291 1106 16-17 0.06877264652570148 1106 18-19 0.10959892570843977 1106 20-21 0.02633535525189501 1106 22-23 0.09800631434468698 1106 24-25 0.14692319041938617 1106 26-27 -0.051439110887997685 1106 28-29 0.06499416470988706 1106 30-31 -0.04960455919948714 1106 32-33 -0.05870420082425909 1106 34-35 -0.2611066737937051 1106 36-37 0.007384462036107209 1106 38-39 -0.0055535800180734896 1106 40-41 -0.04252897310292525 1106 42-43 -0.045915534732110075 1106 44-45 0.03475833250912075 1106 46-47 0.04262161572346912 1106 48-49 -0.0027648380148406204 1106 50-51 -0.0259998724810373 1106 52-53 -0.01439034811687634 1106 54-55 -0.010875439863788472 1106 56-57 -0.04350771659737518 1106 58-59 0.03467774166625048 1106 60-61 0.13437726294230146 1106 62-63 0.18543190277519983 1106 64-65 -0.0633951041496843 1106 66-67 0.11751731231740337 1106 68-69 -0.1963229382889864 1106 70-71 -0.041465162470046835 1106 72-73 0.1792880664907628 1106 74-75 0.1306995222838836 1106 76-77 0.12260436981210887 1106 78-79 0.27300514009261434 1106 80-81 0.15297325698305642 1106 82-83 0.10859080858165271 1106 84-85 0.2047356480542213 1106 86-87 0.328761430058357 1106 88-89 0.44065901260642093 1106 90-91 0.6475651583688702 1106 92-93 0.9211124802905104 1106 94-95 0.6796764382769886 1106 96-97 0.971451593790146 1106 98-99 0.8999093782152165 1106 100-101 1.7428440628891586 1107 1 0.15692161964535245 1107 2 0.1680110161198911 1107 3 0.12478266322482057 1107 4 0.09062452520698372 1107 5 -0.12168323478048393 1107 6 -0.0017710044156800109 1107 7 0.0870363769193574 1107 8 0.08314267689792132 1107 9 0.23003524874748393 1107 10-11 0.171611540422397 1107 12-13 0.13401906924475782 1107 14-15 0.16848189931619117 1107 16-17 0.1750188112600739 1107 18-19 0.15423701981664806 1107 20-21 0.20091879426810522 1107 22-23 0.2492729747385667 1107 24-25 0.19446422264788765 1107 26-27 0.0904232736449373 1107 28-29 0.15558150240144641 1107 30-31 0.17042728884626257 1107 32-33 0.20358122884821483 1107 34-35 0.23584423889722927 1107 36-37 0.2042854651370547 1107 38-39 0.22046455516328223 1107 40-41 0.3368011951987526 1107 42-43 0.36008138887348906 1107 44-45 0.22139411932153052 1107 46-47 0.21076405851153623 1107 48-49 0.18971650725315925 1107 50-51 0.32732913808179376 1107 52-53 0.31271955934583673 1107 54-55 0.253340304566386 1107 56-57 0.3628026760520058 1107 58-59 0.2843158971387183 1107 60-61 0.41543105075290754 1107 62-63 0.388313977050494 1107 64-65 0.5056062335909601 1107 66-67 0.41578817002597646 1107 68-69 0.5031318403497025 1107 70-71 0.5998753191165846 1107 72-73 0.5906325776631505 1107 74-75 0.7484866660644087 1107 76-77 0.6870670030748016 1107 78-79 0.6865947646252266 1107 80-81 0.6583900838785972 1107 82-83 0.7515819884934523 1107 84-85 0.7715691913896556 1107 86-87 0.8355591571265357 1107 88-89 1.0209323494306979 1107 90-91 0.9720400849363209 1107 92-93 1.2944295657340916 1107 94-95 1.299951902395268 1107 96-97 1.582618864553762 1107 98-99 1.7847152830924493 1107 100-101 2.549530413843577 1108 1 0.13173599748053988 1108 2 0.12158513276180827 1108 3 0.03335077893100191 1108 4 0.06391934406008914 1108 5 -0.10649476471396468 1108 6 -0.008510360383048976 1108 7 0.04917657505195194 1108 8 0.11040625020087447 1108 9 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-0.5023134639555487 2316 86-87 -0.47867091380327054 2316 88-89 -0.58446871313798 2316 90-91 -0.7133194673512762 2316 92-93 -0.6770928323576442 2316 94-95 -0.5046701531907019 2316 96-97 -0.7436200440487859 2316 98-99 -0.8360166211424662 2316 100-101 -1.3032706111794319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 20.0 5 51.0 6 98.0 7 183.0 8 472.0 9 969.0 10 1812.0 11 2966.0 12 4769.0 13 6889.0 14 9278.0 15 12900.0 16 18068.0 17 26079.0 18 23673.0 19 13787.0 20 11526.0 21 11276.0 22 11496.0 23 12348.0 24 14413.0 25 17240.0 26 20742.0 27 25831.0 28 32032.0 29 40746.0 30 51626.0 31 66834.0 32 86254.0 33 116945.0 34 165551.0 35 243475.0 36 391373.0 37 633186.0 38 541331.0 39 47753.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.341021467538056 20.277888984113225 15.374628481123423 22.006461067225295 2 15.215046841198635 21.197664715591323 40.39840825885576 23.188880184354286 3 19.06290575716751 25.28587639419054 30.311062195547223 25.340155653094726 4 12.816366366366367 17.500075075075074 37.58111861861862 32.10243993993994 5 13.305288213353156 38.28474244105175 34.23667152857167 14.173297817023425 6 31.099913250783313 37.40313911293577 17.407913885918212 14.089033750362706 7 27.927129156483048 33.68910146805501 20.774166376063672 17.609602999398273 8 25.992670423171766 35.842216275444336 19.72713223455997 18.437981066823923 9 26.251942097619335 17.392748726445674 20.74237209370417 35.61293708223082 10-11 24.431184522828634 27.677373244229265 27.344678173919608 20.5467640590225 12-13 25.406176171435895 24.945272897870208 28.18768125449177 21.460869676202122 14-15 22.323264893669332 26.39490375566676 26.80813558253169 24.47369576813222 16-17 21.28401978828086 29.80625758023364 27.34274499146209 21.566977640023406 18-19 21.259151178997303 28.155807749321415 30.036212448869197 20.548828622812078 20-21 22.055803689152345 27.078533610835887 30.371026721288796 20.494635978722968 22-23 21.813715185758536 27.054526358147683 30.138033240290152 20.99372521580363 24-25 21.608974965352697 27.167502256016167 30.31481373039218 20.908709048238954 26-27 21.417153114722158 27.458643703803386 30.311189741112223 20.81301344036223 28-29 21.19164117653573 27.656258710079562 30.289407848789356 20.86269226459535 30-31 21.438274918449395 27.34576706369018 30.317568759642494 20.898389258217936 32-33 21.420712679912658 27.401322296800938 30.117894099889604 21.0600709233968 34-35 21.695806420493085 27.386551281324596 30.084016484978797 20.833625813203525 36-37 21.68918918918919 27.296921921921925 30.025075075075076 20.988813813813813 38-39 21.4181038220331 27.680845465148096 29.995296548312105 20.905754164506696 40-41 21.733193793846173 27.557347013007877 29.786550380424032 20.922908812721914 42-43 21.474866685775243 27.59514822379408 29.66623250030831 21.26375259012237 44-45 21.742916012418913 27.734993342720216 29.412545265561086 21.10954537929978 46-47 21.836346908278188 28.00648348104974 28.944977873506804 21.212191737165263 48-49 21.8725934923811 28.024018764260745 28.859754343139198 21.243633400218958 50-51 22.001268017542035 28.229080995089717 28.654625129645222 21.115025857723026 52-53 22.03984195948912 28.411481154001283 28.280156103574306 21.268520782935287 54-55 21.845337145143713 28.43193377179663 28.360481095915503 21.362247987144148 56-57 21.91474402599699 28.60815367561799 28.32497059873476 21.152131699650262 58-59 21.976793552254673 28.248675582328985 28.369377488972415 21.405153376443927 60-61 21.93004056680159 28.33399837312373 28.737789512841776 20.998171547232904 62-63 21.76866675350347 28.344002123122326 28.736644618376644 21.15068650499756 64-65 21.77487921363393 28.29231295333598 28.59435863575126 21.33844919727883 66-67 21.790044373106465 28.340942814961405 28.421216808852552 21.447796003079578 68-69 21.742822168610296 28.352410528374428 28.44501559871787 21.459751704297407 70-71 21.93976278537433 28.328161288227744 28.27545860530833 21.456617321089595 72-73 21.967133608959166 28.3420445510845 28.115017353616633 21.575804486339706 74-75 21.764614356952528 28.815275233826142 27.8541823503279 21.565928058893427 76-77 21.800029354343337 28.50072128351303 28.01344669164914 21.685802670494493 78-79 21.84137693642007 28.473018591209236 27.996967718855963 21.688636753514732 80-81 21.816414483327897 28.76190737165639 27.862076628349612 21.559601516666095 82-83 21.891192779568737 28.750118243420786 27.86723403488594 21.491454942124538 84-85 21.80876192077796 28.594645595043684 27.769774963790265 21.826817520388094 86-87 21.585239645180312 28.61018070188412 27.969602864263187 21.83497678867238 88-89 21.786609689711078 28.49323254758538 27.875139686509126 21.845018076194417 90-91 21.848990291648477 28.463590772392255 27.85196199849162 21.835456937467647 92-93 21.734904074724394 28.3236092641584 27.989421614179843 21.952065046937363 94-95 21.59668858983161 28.378743101072413 28.006652399905256 22.017915909190723 96-97 21.466283233377165 28.64413339184182 27.97590916820226 21.91367420657875 98-99 21.48536718075056 28.669109489527848 27.70129592985271 22.144227399868882 100-101 21.348691719025936 28.814749546486624 27.744319069186318 22.092239665301122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 480.0 1 363.0 2 282.0 3 368.0 4 374.0 5 376.0 6 642.0 7 1356.0 8 2263.5 9 2845.5 10 2979.5 11 3057.5 12 3337.0 13 3648.5 14 3958.0 15 4289.5 16 4575.5 17 4880.5 18 5161.5 19 5379.0 20 5889.0 21 6716.5 22 7791.5 23 9433.0 24 11385.0 25 14237.0 26 18188.5 27 22580.0 28 26523.5 29 31163.0 30 37072.0 31 42654.0 32 48468.5 33 54604.0 34 61625.0 35 68212.5 36 75547.5 37 81874.0 38 84297.5 39 85426.5 40 86650.0 41 90341.5 42 93862.0 43 96607.5 44 101083.0 45 104130.0 46 103304.0 47 104091.0 48 107192.0 49 103720.0 50 97582.0 51 91455.5 52 84425.0 53 78309.5 54 73747.0 55 68352.5 56 59773.0 57 51941.5 58 46375.0 59 40534.5 60 32707.0 61 26022.5 62 20935.5 63 15879.5 64 11318.5 65 8153.5 66 5875.5 67 4535.5 68 3345.0 69 2122.0 70 1520.5 71 1101.5 72 849.0 73 664.0 74 499.0 75 363.0 76 225.0 77 136.0 78 85.5 79 49.0 80 28.0 81 17.5 82 12.0 83 4.5 84 3.0 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04816064258234137 2 0.0 3 0.0 4 3.753752344687558E-5 5 0.016366360222837755 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038100586298578714 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.630628517031338E-5 22-23 1.8768761723437791E-4 24-25 4.8798780480938255E-4 26-27 0.0017830323637265902 28-29 0.0023085576919828483 30-31 4.1291275791563144E-4 32-33 0.0 34-35 0.0 36-37 3.753752344687558E-5 38-39 0.0 40-41 0.0 42-43 0.010341587709614223 44-45 0.0 46-47 0.0 48-49 1.876876172343779E-5 50-51 0.0 52-53 1.876876172343779E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1261257034062676E-4 74-75 9.384380861718896E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8768761723437791E-4 84-85 2.8153142585156686E-4 86-87 0.0 88-89 0.0 90-91 0.007957954970737624 92-93 0.002946695590579733 94-95 0.0 96-97 0.0 98-99 2.4399390240469128E-4 100-101 0.013250745776747081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2664001.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.9361637891863 #Duplication Level Percentage of deduplicated Percentage of total 1 71.41597369705472 35.662397597003455 2 14.95123145539666 14.932142856134437 3 5.5828239047502475 8.36354426741379 4 2.63827083848497 5.269804988432774 5 1.4985221545991834 3.741522387689459 6 0.9369944020836081 2.807394355919864 7 0.5830574685012453 2.038095727191056 8 0.42129279806620795 1.6830196933950998 9 0.3002352966274722 1.3493339052915947 >10 1.5103337132589811 13.512409726570185 >50 0.10516474576183753 3.6278344443237915 >100 0.05292738007730679 4.672899325884675 >500 0.0023413453682012784 0.7392976868257936 >1k 6.042181595282885E-4 0.46997186386300105 >5k 1.5105453988207212E-4 0.6419919000622746 >10k+ 7.552726994103606E-5 0.48833927399872695 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12948 0.486035853590145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9980 0.3746244839998183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7042 0.2643392401128979 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2954 0.11088584426207047 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.753752344687558E-5 0.0 6 0.0 0.0 0.0 3.753752344687558E-5 0.0 7 0.0 0.0 0.0 3.753752344687558E-5 0.0 8 0.0 0.0 0.0 3.753752344687558E-5 0.0 9 0.0 0.0 0.0 3.753752344687558E-5 0.0 10-11 0.0 0.0 0.0 7.507504689375117E-5 0.0 12-13 0.0 0.0 0.0 7.507504689375117E-5 0.0 14-15 0.0 0.0 0.0 9.384380861718896E-5 0.0 16-17 0.0 0.0 0.0 1.1261257034062675E-4 0.0 18-19 0.0 0.0 0.0 1.1261257034062675E-4 0.0 20-21 0.0 0.0 0.0 1.5015009378750233E-4 0.0 22-23 0.0 0.0 0.0 2.6276266412812905E-4 0.0 24-25 0.0 0.0 0.0 4.3168151963906924E-4 0.0 26-27 0.0 0.0 0.0 8.633630392781385E-4 0.0 28-29 0.0 0.0 0.0 0.0017642636020031525 0.0 30-31 0.0 0.0 0.0 0.003678677297793807 0.0 32-33 0.0 0.0 0.0 0.006268766415628222 0.0 34-35 0.0 0.0 0.0 0.008596092869334509 0.0 36-37 0.0 0.0 0.0 0.01163663226853143 0.0 38-39 0.0 0.0 0.0 0.014733477952898666 0.0 40-41 0.0 0.0 0.0 0.018975218102395608 0.0 42-43 0.0 0.0 0.0 0.026501491553494162 0.0 44-45 0.0 0.0 0.0 0.037612598493769335 0.0 46-47 0.0 0.0 0.0 0.061280007027024394 0.0 48-49 0.0 0.0 0.0 0.10756377343702198 0.0 50-51 0.0 0.0 0.0 0.14151646339472096 0.0 52-53 0.0 0.0 0.0 0.17349843337145893 0.0 54-55 0.0 0.0 0.0 0.2158970661047049 0.0 56-57 0.0 0.0 0.0 0.2515764821409602 0.0 58-59 0.0 0.0 0.0 0.2972221106523608 0.0 60-61 0.0 0.0 0.0 0.36486472790363067 1.876876172343779E-5 62-63 0.0 0.0 0.0 0.41726711063546895 3.753752344687558E-5 64-65 0.0 0.0 0.0 0.4843842025584826 3.753752344687558E-5 66-67 0.0 0.0 0.0 0.5995117869700499 3.753752344687558E-5 68-69 0.0 0.0 0.0 0.7195004806679878 3.753752344687558E-5 70-71 0.0 0.0 0.0 0.8231603516665347 3.753752344687558E-5 72-73 0.0 0.0 0.0 0.9533404829802992 3.753752344687558E-5 74-75 0.0 0.0 0.0 1.07984193699627 3.753752344687558E-5 76-77 0.0 0.0 0.0 1.1917225256296824 3.753752344687558E-5 78-79 0.0 0.0 0.0 1.3310055063793145 3.753752344687558E-5 80-81 0.0 0.0 0.0 1.503847033090453 3.753752344687558E-5 82-83 0.0 0.0 0.0 1.6842523707761372 3.753752344687558E-5 84-85 0.0 0.0 0.0 1.8877808229050965 3.753752344687558E-5 86-87 0.0 0.0 0.0 2.2095337051299904 3.753752344687558E-5 88-89 0.0 0.0 0.0 2.663362363602716 3.753752344687558E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15270 0.0 32.154076 1 GGTATCA 10520 0.0 31.762472 1 ATCAACG 18790 0.0 25.864458 3 TATCAAC 18945 0.0 25.75315 2 TCAACGC 18930 0.0 25.648077 4 CAACGCA 19175 0.0 25.295122 5 AACGCAG 19680 0.0 24.714275 6 GTACATG 11480 0.0 24.055199 1 TACATGG 11625 0.0 23.416187 2 ACATGGG 11880 0.0 22.11379 3 ACGCAGA 22030 0.0 21.799288 7 GAGTACT 11650 0.0 21.773134 12-13 CGCAGAG 22125 0.0 21.57687 8 AGTACTT 12255 0.0 21.550983 12-13 AGAGTAC 18850 0.0 20.487305 10-11 GTACTTT 12480 0.0 20.361614 14-15 GCAGAGT 23260 0.0 20.156404 9 ATACGTC 445 0.0 19.215818 5 CATGGGG 5595 0.0 18.76492 4 TACTTTT 14435 0.0 18.06461 14-15 >>END_MODULE