FastQCFastQC Report
Thu 26 May 2016
SRR937827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857358
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121360.6534012290576184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80900.4355649260939463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.33504580161713576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22930.12345492899053385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.11187934690027447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38450.057.7560731
GTATCAA81100.049.3119541
ATCAACG108300.036.387383
TATCAAC108800.036.350252
TCAACGC109650.035.940354
CAACGCA112650.034.983225
AACGCAG116800.033.917186
TGGTATC16200.031.103112
GTGGTAT18150.030.1299441
ACGCAGA133000.029.466877
CGCAGAG134100.029.0472558
GTACATG86950.027.2355921
TACATGG86550.027.0215972
GCAGAGT143450.026.9560079
ACATGGG87950.025.240953
GAGTACT92900.021.9867512-13
AGAGTAC138150.021.86886410-11
AGTACTT96750.021.79918712-13
CAGAGTA140050.021.18211610-11
AATACCG1805.1363368E-621.127785
Produced by FastQC (version 0.11.5)