##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937827_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857358 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22675703876151 33.0 31.0 34.0 31.0 34.0 2 32.388222410542284 34.0 31.0 34.0 31.0 34.0 3 32.42551947443627 34.0 31.0 34.0 31.0 34.0 4 35.993140794612565 37.0 35.0 37.0 35.0 37.0 5 35.98867800391739 37.0 35.0 37.0 35.0 37.0 6 36.07263004762679 37.0 37.0 37.0 35.0 37.0 7 36.03586653730729 37.0 37.0 37.0 35.0 37.0 8 36.03354819049424 37.0 37.0 37.0 35.0 37.0 9 37.749184594461596 39.0 38.0 39.0 35.0 39.0 10-11 37.64644376582221 39.0 38.0 39.0 35.0 39.0 12-13 37.55479772881695 39.0 37.5 39.0 35.0 39.0 14-15 38.81199558728043 40.0 38.0 41.0 36.0 41.0 16-17 38.73962531725171 40.0 38.0 41.0 36.0 41.0 18-19 38.68513932155244 40.0 38.0 41.0 35.0 41.0 20-21 38.60313089883587 40.0 38.0 41.0 35.0 41.0 22-23 38.52637725198912 40.0 38.0 41.0 35.0 41.0 24-25 38.5680272731482 40.0 38.0 41.0 35.0 41.0 26-27 38.48388813572828 40.0 38.0 41.0 35.0 41.0 28-29 38.358303299633135 40.0 38.0 41.0 34.5 41.0 30-31 38.1081016691451 40.0 38.0 41.0 34.0 41.0 32-33 37.957583298427124 40.0 38.0 41.0 34.0 41.0 34-35 37.95845900467222 40.0 38.0 41.0 34.0 41.0 36-37 37.82759381874684 40.0 38.0 41.0 34.0 41.0 38-39 37.6883578717727 40.0 37.5 41.0 33.0 41.0 40-41 37.45770120784469 40.0 37.0 41.0 33.0 41.0 42-43 37.1764046026668 40.0 37.0 41.0 33.0 41.0 44-45 36.90475099576926 40.0 37.0 41.0 31.5 41.0 46-47 36.50564376926796 39.5 36.0 41.0 31.0 41.0 48-49 36.15199466123386 39.0 36.0 41.0 30.0 41.0 50-51 35.27811385850224 38.5 35.0 39.5 28.5 40.5 52-53 35.38074619970948 38.5 35.0 40.0 29.0 40.5 54-55 35.67157866173349 39.0 35.0 40.0 29.0 41.0 56-57 35.46293579374574 39.0 35.0 40.0 28.0 41.0 58-59 35.22985714116503 38.5 35.0 40.0 27.5 41.0 60-61 34.92022028063518 38.0 35.0 40.0 26.5 41.0 62-63 34.560391965361546 38.0 34.0 40.0 26.0 41.0 64-65 34.183504472481886 37.5 34.0 40.0 26.0 41.0 66-67 33.75253693687485 37.0 34.0 39.5 24.5 41.0 68-69 33.25996253818596 36.0 33.0 39.0 23.0 40.5 70-71 32.80999597277423 35.5 33.0 39.0 22.0 40.0 72-73 32.35304906216249 35.0 32.0 38.0 20.5 40.0 74-75 31.822474719467113 35.0 31.5 37.0 18.5 39.0 76-77 31.335512055295748 35.0 31.0 37.0 17.0 39.0 78-79 30.787987022426478 34.5 31.0 36.0 10.5 38.5 80-81 30.238939396712965 34.0 30.0 36.0 7.0 37.5 82-83 29.74885617096973 34.0 29.5 35.0 4.5 37.0 84-85 29.240512599078905 34.0 29.0 35.0 2.0 36.5 86-87 28.71554406851022 34.0 29.0 35.0 2.0 36.0 88-89 28.266253463252642 33.5 28.5 35.0 2.0 36.0 90-91 27.726034776278993 33.0 26.5 35.0 2.0 35.0 92-93 27.11600079252357 33.0 26.0 35.0 2.0 35.0 94-95 26.39258209779698 32.0 25.0 34.0 2.0 35.0 96-97 25.569390230639435 32.0 22.5 34.0 2.0 35.0 98-99 24.509251851285534 31.5 10.0 34.0 2.0 35.0 100-101 21.559213678784594 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2438071877455812 1101 2 0.3924769426164971 1101 3 0.4049037577988486 1101 4 0.32954314188478406 1101 5 0.24585606697667117 1101 6 0.26737153988594997 1101 7 0.2917781163253679 1101 8 0.30223982944747974 1101 9 0.3821940809989286 1101 10-11 0.4229311854847637 1101 12-13 0.36386203530393857 1101 14-15 0.4777032424271255 1101 16-17 0.49294322817558367 1101 18-19 0.5178879623305193 1101 20-21 0.5246528670918309 1101 22-23 0.4608398686277937 1101 24-25 0.3889744405109923 1101 26-27 0.502781443696243 1101 28-29 0.5673048986607796 1101 30-31 0.625314229017846 1101 32-33 0.5798082642961191 1101 34-35 0.5844654119483934 1101 36-37 0.6269757751476845 1101 38-39 0.6110283067242719 1101 40-41 0.6290337869199121 1101 42-43 0.6604993868199927 1101 44-45 0.7215818710219963 1101 46-47 0.8987897072732878 1101 48-49 0.9071364223850082 1101 50-51 0.7991466321396032 1101 52-53 0.6491612050534386 1101 54-55 0.7112507443091545 1101 56-57 0.7135340307119336 1101 58-59 0.7340877474572309 1101 60-61 0.8337645645465344 1101 62-63 0.8569834149564102 1101 64-65 0.8760018189669481 1101 66-67 0.9153117392466257 1101 68-69 0.8571151318791621 1101 70-71 0.8942939499572446 1101 72-73 0.9504911985591917 1101 74-75 0.8526036055055357 1101 76-77 0.9054978324655352 1101 78-79 0.9656486860832381 1101 80-81 1.0042398033448627 1101 82-83 1.146867709215094 1101 84-85 1.1917097384049207 1101 86-87 1.1974444275605869 1101 88-89 1.181736841961932 1101 90-91 1.0508537762311008 1101 92-93 1.1047472553052593 1101 94-95 1.0724020342029412 1101 96-97 1.3667204743175851 1101 98-99 1.3769293113677321 1101 100-101 1.6304459990609175 1102 1 0.20540615799729522 1102 2 0.2287562557264735 1102 3 0.2430227802714171 1102 4 0.08733470831479906 1102 5 0.09694167886033966 1102 6 0.09477563051945026 1102 7 0.07201902657200066 1102 8 0.09374208354984859 1102 9 0.15666160432228793 1102 10-11 0.14513079333940482 1102 12-13 0.18388864691377904 1102 14-15 0.22802289956886312 1102 16-17 0.32485827394217637 1102 18-19 0.304074923252891 1102 20-21 0.24592100748182588 1102 22-23 0.28068495409787175 1102 24-25 0.3839901116565301 1102 26-27 0.2926982472715167 1102 28-29 0.3245142979253828 1102 30-31 0.3532835566010206 1102 32-33 0.3535295134893346 1102 34-35 0.36335679452970027 1102 36-37 0.5443413480123951 1102 38-39 0.6745865980071031 1102 40-41 0.5512370008493832 1102 42-43 0.5547564376130794 1102 44-45 0.5120227778459139 1102 46-47 0.5542451484123632 1102 48-49 0.5101253083401431 1102 50-51 0.46009186380851474 1102 52-53 0.5544134224838544 1102 54-55 0.5427489522733282 1102 56-57 0.5633712683852679 1102 58-59 0.45623815378240806 1102 60-61 0.6014434992020128 1102 62-63 0.6406241203290932 1102 64-65 0.4741417566735393 1102 66-67 0.5533999572833608 1102 68-69 0.4518818616215796 1102 70-71 0.3603436722283675 1102 72-73 0.407734763854819 1102 74-75 0.38137549865814435 1102 76-77 0.4326573823296016 1102 78-79 0.6482887362504641 1102 80-81 0.6295035357667764 1102 82-83 0.7285990896449874 1102 84-85 0.8454299852315721 1102 86-87 0.7503186525601002 1102 88-89 0.8520386154173103 1102 90-91 1.080435813822291 1102 92-93 1.3602199322596285 1102 94-95 1.41127222039864 1102 96-97 1.406648585076642 1102 98-99 1.6262340723644257 1102 100-101 1.8644295260221355 1103 1 0.20100847634883223 1103 2 0.22028515560706552 1103 3 0.2040805551971232 1103 4 0.11662194219967859 1103 5 0.12310765433971227 1103 6 0.14365925588992923 1103 7 0.21632755687668492 1103 8 0.2005586603481504 1103 9 0.2421931776215871 1103 10-11 0.2239578899642254 1103 12-13 0.2847711474775849 1103 14-15 0.2386264456741216 1103 16-17 0.3370124349457555 1103 18-19 0.32739693641649126 1103 20-21 0.27650519393375816 1103 22-23 0.20152237827456787 1103 24-25 0.21181367483417546 1103 26-27 0.2739281360446384 1103 28-29 0.27812433450163354 1103 30-31 0.42144011142392657 1103 32-33 0.33881179069409484 1103 34-35 0.4207097174447938 1103 36-37 0.3042105451736248 1103 38-39 0.28483427579005394 1103 40-41 0.44966882086108484 1103 42-43 0.4759333467100362 1103 44-45 0.4504837755350124 1103 46-47 0.5518601416687261 1103 48-49 0.4464918142571932 1103 50-51 0.45074020590035957 1103 52-53 0.49198625151283437 1103 54-55 0.50740626878423 1103 56-57 0.514488344016101 1103 58-59 0.49763578616050097 1103 60-61 0.4642457259026358 1103 62-63 0.5974929117107024 1103 64-65 0.6562562890716137 1103 66-67 0.7163614314530591 1103 68-69 0.8183447171644289 1103 70-71 0.927481371718315 1103 72-73 0.6477513034800211 1103 74-75 0.7611662918271094 1103 76-77 0.6918521160873041 1103 78-79 0.7883211585624927 1103 80-81 0.8273038737695764 1103 82-83 0.8451513729264235 1103 84-85 0.7452082284742225 1103 86-87 0.7499673507606097 1103 88-89 0.8744371094261538 1103 90-91 0.8236027534788946 1103 92-93 0.889261915828957 1103 94-95 1.0527588198573063 1103 96-97 1.1846304543787554 1103 98-99 0.9930994028024251 1103 100-101 1.1768106311927617 1104 1 0.18631850203428968 1104 2 0.3140290275848443 1104 3 0.12181436837803261 1104 4 0.1028453445740567 1104 5 0.14223712960420443 1104 6 0.07175043121645786 1104 7 0.14402854796875175 1104 8 0.17254897767120525 1104 9 0.2153508329478626 1104 10-11 0.21363809962697644 1104 12-13 0.35033475675007253 1104 14-15 0.3453080794490475 1104 16-17 0.283144105850468 1104 18-19 0.23393937367357864 1104 20-21 0.2705473499045752 1104 22-23 0.3141980214557023 1104 24-25 0.22753817985071123 1104 26-27 0.19164667939969604 1104 28-29 0.35225411516263705 1104 30-31 0.38819649501481024 1104 32-33 0.37505070399684826 1104 34-35 0.31246794948828693 1104 36-37 0.31242792159251564 1104 38-39 0.36657345269821917 1104 40-41 0.39687259427615373 1104 42-43 0.3002095971480401 1104 44-45 0.3158117472842221 1104 46-47 0.3744852417262763 1104 48-49 0.29319401407652634 1104 50-51 0.2772227452563243 1104 52-53 0.2801330893730025 1104 54-55 0.3106750562957643 1104 56-57 0.26997222068734317 1104 58-59 0.3172322368100353 1104 60-61 0.2622213086474119 1104 62-63 0.2882188633423226 1104 64-65 0.3880150756508911 1104 66-67 0.38833402774136516 1104 68-69 0.2698356951083767 1104 70-71 0.20610887958629576 1104 72-73 0.2621524369832997 1104 74-75 0.32133643787094 1104 76-77 0.3535575627538208 1104 78-79 0.482663446978961 1104 80-81 0.47543987005338195 1104 82-83 0.5963114778542362 1104 84-85 0.7024125323148205 1104 86-87 0.7303359804303646 1104 88-89 0.8487689339935365 1104 90-91 0.8171319638520913 1104 92-93 0.7702203626144204 1104 94-95 0.769272292665768 1104 96-97 0.8583745513855483 1104 98-99 1.148594048719275 1104 100-101 1.3584894119212763 1105 1 0.09881938929227374 1105 2 0.15165916563393012 1105 3 0.13357590149380627 1105 4 0.09460993976156828 1105 5 0.10285257301034534 1105 6 0.08152471278509665 1105 7 0.08296889782076278 1105 8 0.09203712472987746 1105 9 0.06664866440681294 1105 10-11 0.13841832788749997 1105 12-13 0.12555073185072274 1105 14-15 0.22665393426227354 1105 16-17 0.20497523300650045 1105 18-19 0.2637631064628394 1105 20-21 0.21055560759401004 1105 22-23 0.2512276715541688 1105 24-25 0.22048437570114743 1105 26-27 0.19030815787687771 1105 28-29 0.13178267991761317 1105 30-31 0.14511937702683042 1105 32-33 0.19838381448398934 1105 34-35 0.31347055101122834 1105 36-37 0.3386698145306042 1105 38-39 0.44458746807295313 1105 40-41 0.4915939870153352 1105 42-43 0.4019228923169038 1105 44-45 0.38138516767613595 1105 46-47 0.42585489981419045 1105 48-49 0.3614930703641761 1105 50-51 0.2787562571967541 1105 52-53 0.35930023061952454 1105 54-55 0.4534598330946693 1105 56-57 0.5574464902057841 1105 58-59 0.5576924595901929 1105 60-61 0.7122417568488757 1105 62-63 0.6771724395284835 1105 64-65 0.6118815107225899 1105 66-67 0.7140048566796793 1105 68-69 0.6695371637473144 1105 70-71 0.7522546515337538 1105 72-73 0.7376729891796572 1105 74-75 0.7235483307595061 1105 76-77 0.6865102005099075 1105 78-79 0.7075726165219969 1105 80-81 0.7311050122870277 1105 82-83 0.7489873241332852 1105 84-85 0.6946781187552098 1105 86-87 0.6896917238370044 1105 88-89 0.9403956534169744 1105 90-91 0.997326013363562 1105 92-93 1.085916864259353 1105 94-95 1.4397400344415026 1105 96-97 1.602310658906358 1105 98-99 1.589509888791639 1105 100-101 1.6462651428155155 1106 1 0.08843736329840368 1106 2 0.07726859421166665 1106 3 0.002384707423871646 1106 4 0.09082566618004506 1106 5 0.011796438097263717 1106 6 0.08067108291809433 1106 7 0.11937674421608335 1106 8 0.18146512424600303 1106 9 0.17569645626719677 1106 10-11 0.11281323593208015 1106 12-13 0.09884969174609637 1106 14-15 0.08866489450458204 1106 16-17 0.08508246169942169 1106 18-19 0.13562086220753145 1106 20-21 0.1279612118795086 1106 22-23 0.2165681686832457 1106 24-25 0.04852208453424112 1106 26-27 0.17327702473268403 1106 28-29 0.2520162945227469 1106 30-31 0.2135299745144863 1106 32-33 0.31690027881299443 1106 34-35 0.38987879302823103 1106 36-37 0.24222865983263375 1106 38-39 0.2927630814957851 1106 40-41 0.4071485383004543 1106 42-43 0.3131879915608806 1106 44-45 0.20380390736239207 1106 46-47 0.3443853630643616 1106 48-49 0.35694456220029025 1106 50-51 0.3914407253176364 1106 52-53 0.2791427123239245 1106 54-55 0.3125519421919165 1106 56-57 0.3693405518482038 1106 58-59 0.46054945680638326 1106 60-61 0.35852477699439333 1106 62-63 0.4510495396519687 1106 64-65 0.5587765271827294 1106 66-67 0.6464859296056886 1106 68-69 0.43754004265588264 1106 70-71 0.42076628471332356 1106 72-73 0.5291290063192093 1106 74-75 0.442776761959756 1106 76-77 0.4306540485546577 1106 78-79 0.5118281363955184 1106 80-81 0.38224619064621734 1106 82-83 0.35784985853144846 1106 84-85 0.32291166032249663 1106 86-87 0.6499880444792545 1106 88-89 0.7975534007166658 1106 90-91 0.6409572270015573 1106 92-93 0.7968018623611322 1106 94-95 0.9502015651778883 1106 96-97 1.1442994339018533 1106 98-99 1.3816319959031702 1106 100-101 1.445517950398063 1107 1 0.19381056963183596 1107 2 0.2062995343478775 1107 3 0.14391079943443685 1107 4 0.1301160516962767 1107 5 0.11959608584211168 1107 6 0.09710042229136917 1107 7 0.17380055742570732 1107 8 0.18551768156581971 1107 9 0.19121073815975365 1107 10-11 0.2555703181132003 1107 12-13 0.24937398890079976 1107 14-15 0.1675755899314737 1107 16-17 0.283291187709942 1107 18-19 0.3192247671243891 1107 20-21 0.33413247705981064 1107 22-23 0.2813231767340838 1107 24-25 0.23110753327338784 1107 26-27 0.2796020867299305 1107 28-29 0.24350779903954844 1107 30-31 0.3352256235980562 1107 32-33 0.22018807754287906 1107 34-35 0.19311079284018007 1107 36-37 0.2658883524740503 1107 38-39 0.4278107344433053 1107 40-41 0.4964051198583874 1107 42-43 0.38009394435958654 1107 44-45 0.3566834281521096 1107 46-47 0.37615254893519534 1107 48-49 0.2079803401082856 1107 50-51 0.12418657495830132 1107 52-53 0.11978335330070422 1107 54-55 0.17894207680890162 1107 56-57 0.2282824823564411 1107 58-59 0.21326972401435995 1107 60-61 0.38314695899511975 1107 62-63 0.4487081678045044 1107 64-65 0.44333083262611694 1107 66-67 0.5288252082941369 1107 68-69 0.48501235726999425 1107 70-71 0.47786447233984575 1107 72-73 0.46380257705259 1107 74-75 0.47149221714068545 1107 76-77 0.48013948962635666 1107 78-79 0.5703242352284406 1107 80-81 0.6912647397288403 1107 82-83 0.7449611479901534 1107 84-85 0.6639517644655513 1107 86-87 0.7416790427202145 1107 88-89 0.7892454063603616 1107 90-91 0.8176059954797026 1107 92-93 0.9971804219207385 1107 94-95 1.227121045156597 1107 96-97 1.4289538080165265 1107 98-99 1.5818330007314287 1107 100-101 1.422714550078048 1108 1 0.030487757344502597 1108 2 0.03333471140651767 1108 3 0.21530567893130126 1108 4 0.09181142634720629 1108 5 0.05445692643066735 1108 6 0.12974304308784212 1108 7 0.11805465828694395 1108 8 0.06101342230221718 1108 9 0.08870739410981088 1108 10-11 0.16182779822490545 1108 12-13 0.17203843402317887 1108 14-15 0.14174309749958525 1108 16-17 0.19195843714087601 1108 18-19 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-0.6550221398087146 2316 60-61 -0.6549300353670091 2316 62-63 -0.6189763460382949 2316 64-65 -0.5227781988930289 2316 66-67 -0.5383816946486775 2316 68-69 -0.6847715851822471 2316 70-71 -0.6810670891072093 2316 72-73 -0.5819831205999044 2316 74-75 -0.4467456283351581 2316 76-77 -0.624323439688812 2316 78-79 -0.7177499869311177 2316 80-81 -0.6544641673738631 2316 82-83 -0.7951989585891539 2316 84-85 -0.924856536934648 2316 86-87 -0.915104061734791 2316 88-89 -0.8138155805120739 2316 90-91 -0.6341620361139064 2316 92-93 -0.9534327892878309 2316 94-95 -0.5987161204167677 2316 96-97 -0.9697273364120598 2316 98-99 -1.0920130105443064 2316 100-101 -1.1357908050252625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6653.0 3 982.0 4 785.0 5 828.0 6 958.0 7 1060.0 8 1324.0 9 1584.0 10 2044.0 11 2935.0 12 4465.0 13 6663.0 14 7395.0 15 10374.0 16 14532.0 17 21268.0 18 16918.0 19 9846.0 20 9106.0 21 9478.0 22 9856.0 23 10812.0 24 12231.0 25 14062.0 26 16710.0 27 19898.0 28 24146.0 29 30063.0 30 37620.0 31 48775.0 32 66222.0 33 92510.0 34 134534.0 35 204166.0 36 308852.0 37 410888.0 38 267477.0 39 19336.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.56800757697662 19.081053994598058 14.96463681594189 23.386301612483436 2 14.487214020556674 19.86481241670207 41.84477826893606 23.8031952938052 3 18.622775889077577 24.828478552126864 29.780657670719847 26.76808788807571 4 12.08705551737931 16.332356105389472 37.62341978678761 33.95716859044361 5 13.272409053561034 37.267336589010526 34.179211964883606 15.281042392544839 6 31.023408672519686 36.315886278695835 17.866622509197825 14.79408253958665 7 27.83930656727608 33.19383051999363 20.865172751373407 18.101690161356878 8 25.754069710993882 34.682136422969464 20.075946362881215 19.487847503155443 9 25.997051765623457 16.950028344258413 20.90182746302436 36.151092427093765 10-11 24.081003178017816 27.04284673582265 27.45623448986786 21.419915596291673 12-13 25.087757338822076 24.40077433580586 28.514581634282216 21.996886691089852 14-15 22.134353986878466 25.45551437977471 27.237765547602976 25.172366085743853 16-17 20.976413731834544 29.049221464089285 27.749823142769834 22.224541661306336 18-19 20.828751974467906 27.030570170506106 30.994123185222282 21.1465546698037 20-21 21.497108447056064 26.136105916188786 31.19778653610969 21.168999100645458 22-23 21.173971233338843 26.119537215792565 31.207623216443547 21.49886833442504 24-25 20.8578256016556 26.286095223404278 31.38832563900583 21.467753535934296 26-27 20.680407528287166 26.61745642957824 31.07879148085636 21.62334456127823 28-29 20.60863282538417 26.666724201215942 31.314994375548324 21.409648597851568 30-31 20.779761594919577 26.436279085573545 31.210637903640592 21.573321415866285 32-33 20.551400482785592 26.500074170970834 31.174059040092782 21.774466306150796 34-35 21.02048962572787 26.634652469300622 30.951228919042112 21.393628985929396 36-37 20.99645596326045 26.434097176662263 30.86774295581239 21.701703904264896 38-39 20.74193791056656 26.623115569759815 30.96611874576508 21.668827773908546 40-41 20.982735365670166 26.585827284626205 30.88025204759005 21.551185302113574 42-43 20.9326702928938 26.546868131719958 30.673539778381247 21.846921797004992 44-45 20.824184329570173 26.839145276740844 30.55780741057169 21.77886298311729 46-47 20.980968757348958 27.058301923836193 29.958106886500786 22.002622432314062 48-49 21.172417419867234 27.16025743885288 29.71854592624455 21.948779215035337 50-51 21.22872197833079 27.359305748160565 29.42660252313013 21.985369750378513 52-53 21.36161828798571 27.397235146851635 29.31977620707077 21.921370358091885 54-55 21.109671399736538 27.4356343396781 29.381267182715554 22.07342707786981 56-57 21.160156580720553 27.70671475464293 29.23167270552568 21.90145595911084 58-59 21.252784959721048 27.37272636333266 29.379326411165348 21.995162265780944 60-61 21.241793411002156 27.340539403760133 29.59173989123489 21.82592729400282 62-63 21.12304872347 27.4624493694061 29.657219217596552 21.757282689527347 64-65 21.045800026261887 27.367742154666324 29.566379610423542 22.02007820864825 66-67 20.95747402910272 27.57572873411609 29.384854603688517 22.081942633092673 68-69 21.010526781286764 27.50167788356661 29.443357913529876 22.04443742161675 70-71 21.061373460917885 27.545714082331724 29.29586723556261 22.097045221187777 72-73 21.133196598102224 27.582059338273023 29.09184493070802 22.19289913291673 74-75 20.987760510125693 27.789436446355133 29.04978600508583 22.17301703843334 76-77 21.024550568659972 27.62671724417986 29.08051515505317 22.268217032106996 78-79 21.141232319500826 27.689397565345885 29.004067643961832 22.165302471191453 80-81 20.982473037360634 27.930271792895866 28.929505225894598 22.157749943848902 82-83 21.137530950146232 27.854983313951436 28.858330230342155 22.149155505560174 84-85 21.155706491554067 27.678508839569872 28.83291579087 22.332868878006064 86-87 21.053745467290348 27.650189396492458 28.964425811843686 22.33163932437351 88-89 21.15007260681938 27.583500538417443 28.97521662067965 22.291210234083522 90-91 21.066778834292556 27.698204374267775 28.96645352121411 22.26856327022556 92-93 21.143230133482806 27.462505564142738 29.01833558595175 22.375928716422706 94-95 20.97918011420227 27.42221460082861 28.9466461104739 22.65195917449522 96-97 21.092234645758456 27.367474541037662 28.94254646243775 22.597744350766128 98-99 21.04420362615117 27.409596973282184 28.78676760040077 22.759431800165874 100-101 21.26480581025864 26.913422757457393 29.23231787879515 22.589453553488816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2734.0 1 1659.5 2 669.0 3 762.0 4 615.0 5 564.5 6 1084.0 7 2402.0 8 3854.5 9 4200.5 10 3431.0 11 2804.5 12 2776.5 13 2978.0 14 3229.5 15 3296.0 16 3449.5 17 3718.0 18 3891.5 19 4072.0 20 4441.0 21 5091.5 22 5934.0 23 6883.5 24 8017.5 25 9631.5 26 12146.5 27 15003.5 28 17325.5 29 20147.0 30 23770.5 31 27424.5 32 31795.0 33 36284.0 34 41286.5 35 45622.5 36 50120.5 37 54689.0 38 56695.5 39 58342.0 40 60673.5 41 63552.5 42 65994.5 43 68356.0 44 70670.0 45 72125.0 46 71271.5 47 70174.5 48 70620.5 49 69128.0 50 65903.0 51 61825.0 52 57873.0 53 55156.0 54 52485.5 55 47954.0 56 42304.5 57 37284.0 58 33551.5 59 29932.0 60 25266.5 61 20563.0 62 16168.5 63 11993.5 64 8968.5 65 7027.0 66 5097.5 67 3785.0 68 2778.0 69 1874.5 70 1370.5 71 1014.0 72 799.0 73 640.5 74 491.5 75 380.0 76 248.0 77 165.5 78 110.0 79 76.5 80 61.0 81 41.5 82 27.5 83 18.5 84 18.0 85 16.5 86 10.0 87 6.0 88 7.0 89 8.0 90 7.5 91 4.0 92 1.5 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29245842750832096 2 0.25956223840530473 3 0.25950839848860585 4 0.2605313569058846 5 0.26941494316119996 6 0.18235579785910955 7 0.18267883735930285 8 0.18402483527677488 9 0.18127899952513193 10-11 0.19002798598870008 12-13 0.18208659827561516 14-15 0.1821135182339646 16-17 0.1863668716531762 18-19 0.18184431865047018 20-21 0.17500664922971232 22-23 0.17403753072913244 24-25 0.176944886230872 26-27 0.1725300130615638 28-29 0.18302879681784556 30-31 0.188251268737637 32-33 0.19021642569714617 34-35 0.18806282902919094 36-37 0.16779210039206227 38-39 0.16068523138781 40-41 0.16717294135002514 42-43 0.17621804735543714 44-45 0.17934076252397224 46-47 0.18057908060804648 48-49 0.17904464298212838 50-51 0.18257115752590508 52-53 0.2029495659964315 54-55 0.17258385297826268 56-57 0.16038911184596613 58-59 0.16030835197091783 60-61 0.1562972781768512 62-63 0.16125055051314824 64-65 0.15933923347033796 66-67 0.1575625162192749 68-69 0.16623074280779473 70-71 0.16184278959683593 72-73 0.15734715655247938 74-75 0.16106211080470217 76-77 0.1638079465563451 78-79 0.16639226255789138 80-81 0.16079291122120776 82-83 0.15960843305383238 84-85 0.1537668020920038 86-87 0.1498364881729855 88-89 0.15338992267511165 90-91 0.1610082708880033 92-93 0.1534168426334611 94-95 0.1576971160110221 96-97 0.1562972781768512 98-99 0.1582893550947098 100-101 0.1420566202099972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46262208504498 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76421383051006 39.97090909506052 2 13.861952647301681 14.821926714869571 3 4.77123440939193 7.652481063254505 4 2.1483129554713636 4.594177746350862 5 1.210559931198116 3.235985405647145 6 0.6968370083114687 2.2352840178137634 7 0.46824921430885424 1.7523681550349457 8 0.34276709490140056 1.466018212632186 9 0.23434148586506734 1.1275659267886804 >10 1.3547390764728222 13.209647657352228 >50 0.10302580905873439 3.770841019213485 >100 0.041750087259506345 3.8559360793061424 >500 0.0014115149642907783 0.5507742203522484 >1k 3.024674923480239E-4 0.3304214103307186 >5k 2.0164499489868263E-4 0.7715043468292946 >10k+ 1.0082249744934131E-4 0.6541589291637195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12136 0.6534012290576184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8090 0.4355649260939463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6223 0.33504580161713576 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2293 0.12345492899053385 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.11187934690027447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.3839916698880884E-5 0.0 16-17 0.0 0.0 0.0 5.3839916698880884E-5 0.0 18-19 0.0 0.0 0.0 5.3839916698880884E-5 0.0 20-21 0.0 0.0 0.0 1.0767983339776177E-4 0.0 22-23 0.0 0.0 0.0 2.4227962514496398E-4 0.0 24-25 0.0 0.0 0.0 4.307193335910471E-4 0.0 26-27 0.0 0.0 0.0 9.691185005798559E-4 0.0 28-29 0.0 0.0 0.0 0.00183055716776195 0.0 30-31 0.0 0.0 0.0 0.0033919147520294957 0.0 32-33 0.0 0.0 0.0 0.005572431378334171 0.0 34-35 0.0 0.0 0.0 0.008641306630170383 0.0 36-37 0.0 0.0 0.0 0.013217699549575256 0.0 38-39 0.0 0.0 0.0 0.021132167304310744 0.0 40-41 0.0 0.0 0.0 0.03173863089399028 0.0 42-43 0.0 0.0 0.0 0.0402722576907629 0.0 44-45 0.0 0.0 0.0 0.05332843749024151 0.0 46-47 0.0 0.0 0.0 0.07053029087553396 0.0 48-49 0.0 0.0 0.0 0.0863323064266555 0.0 50-51 0.0 0.0 0.0 0.1074375537726168 0.0 52-53 0.0 0.0 0.0 0.12875816078537364 0.0 54-55 0.0 0.0 0.0 0.1511017262154092 0.0 56-57 0.0 0.0 0.0 0.18526315336084914 0.0 58-59 0.0 0.0 0.0 0.22322029463356013 0.0 60-61 0.0 0.0 0.0 0.2623619140736465 0.0 62-63 0.0 0.0 0.0 0.3046800885989669 0.0 64-65 0.0 0.0 0.0 0.35179001571048774 0.0 66-67 0.0 0.0 0.0 0.40964100620343524 0.0 68-69 0.0 0.0 0.0 0.47659094261849355 0.0 70-71 0.0 0.0 0.0 0.5567316586247778 0.0 72-73 0.0 0.0 0.0 0.6441946033021098 0.0 74-75 0.0 0.0 0.0 0.7410526134433965 0.0 76-77 0.0 0.0 0.0 0.8527704405935743 0.0 78-79 0.0 0.0 0.0 0.979698044211186 0.0 80-81 0.0 0.0 0.0 1.1205701862538078 0.0 82-83 0.0 0.0 0.0 1.2766790247222128 0.0 84-85 0.0 0.0 0.0 1.4411868901956435 0.0 86-87 0.0 0.0 0.0 1.6199623335942777 2.6919958349440442E-5 88-89 0.0 0.0 0.0 1.817177948462278 5.3839916698880884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3845 0.0 57.756073 1 GTATCAA 8110 0.0 49.311954 1 ATCAACG 10830 0.0 36.38738 3 TATCAAC 10880 0.0 36.35025 2 TCAACGC 10965 0.0 35.94035 4 CAACGCA 11265 0.0 34.98322 5 AACGCAG 11680 0.0 33.91718 6 TGGTATC 1620 0.0 31.10311 2 GTGGTAT 1815 0.0 30.129944 1 ACGCAGA 13300 0.0 29.46687 7 CGCAGAG 13410 0.0 29.047255 8 GTACATG 8695 0.0 27.235592 1 TACATGG 8655 0.0 27.021597 2 GCAGAGT 14345 0.0 26.956007 9 ACATGGG 8795 0.0 25.24095 3 GAGTACT 9290 0.0 21.98675 12-13 AGAGTAC 13815 0.0 21.868864 10-11 AGTACTT 9675 0.0 21.799187 12-13 CAGAGTA 14005 0.0 21.182116 10-11 AATACCG 180 5.1363368E-6 21.12778 5 >>END_MODULE