FastQCFastQC Report
Thu 26 May 2016
SRR937827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857358
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124620.6709530419014536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93680.5043723396351161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73700.3968001860707521No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.1366457085817597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.10380335939544234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.055.0735861
GTATCAA100200.041.684061
TATCAAC136100.030.6489122
ATCAACG139100.030.056213
TCAACGC143250.029.2186344
CAACGCA145900.028.655375
AACGCAG150450.027.8135956
TGGTATC24650.025.2483522
ACGCAGA176200.023.5601987
CGCAGAG176600.023.4799398
TGCACCG2903.6379788E-1222.9355265
GTGGTAT29500.022.8725811
GCAGAGT184500.022.0369179
GAGTACT113850.021.23528912-13
AGTACTT116100.021.08967412-13
GTACTTT120700.019.87272814-15
AGAGTAC174350.019.80550210-11
CAGAGTA181350.019.26364910-11
TACTTTT129650.018.88554814-15
GTACATG96450.018.0313261
Produced by FastQC (version 0.11.5)