##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857358 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96879007708799 31.0 31.0 33.0 28.0 34.0 2 31.318775378790733 31.0 31.0 34.0 30.0 34.0 3 31.357730712119043 31.0 31.0 34.0 28.0 34.0 4 35.23456598027951 37.0 35.0 37.0 33.0 37.0 5 35.06699785394092 35.0 35.0 37.0 32.0 37.0 6 35.176329495983005 37.0 35.0 37.0 32.0 37.0 7 35.0950995984619 37.0 35.0 37.0 32.0 37.0 8 35.05645007586044 37.0 35.0 37.0 32.0 37.0 9 36.521882157343924 38.0 35.0 39.0 32.0 39.0 10-11 36.37432417444564 38.0 35.0 39.0 32.0 39.0 12-13 36.187444208386324 38.0 35.0 39.0 32.0 39.0 14-15 36.984508909967815 38.0 36.0 40.0 31.0 41.0 16-17 36.893367622181614 38.0 36.0 40.0 31.0 41.0 18-19 36.848572273088976 38.5 36.0 40.0 31.0 41.0 20-21 36.704046015900005 38.0 35.5 40.0 30.5 41.0 22-23 36.59769925883971 38.0 35.0 40.0 30.0 41.0 24-25 36.59014201893227 38.0 35.0 40.0 30.0 41.0 26-27 36.2984411190519 38.0 35.0 40.0 30.0 41.0 28-29 36.02693880232029 38.0 34.0 40.0 29.0 41.0 30-31 35.982360697291526 38.0 35.0 40.0 30.0 41.0 32-33 35.80016157359002 38.0 34.0 40.0 29.0 41.0 34-35 35.5411506559317 38.0 34.0 40.0 28.5 41.0 36-37 35.248593701375825 38.0 34.0 40.0 27.0 41.0 38-39 35.001251239664086 38.0 33.5 40.0 27.0 41.0 40-41 34.65869638486495 37.5 33.0 40.0 25.5 41.0 42-43 34.27380558836799 37.0 33.0 40.0 24.5 41.0 44-45 33.73091751832442 37.0 32.5 39.5 23.5 40.0 46-47 33.13210000441487 37.0 31.5 39.0 21.0 40.0 48-49 32.592718797345476 36.5 31.0 39.0 18.5 40.0 50-51 32.122459698130356 36.0 30.5 39.0 15.0 40.0 52-53 31.693806202143044 36.0 30.0 39.0 12.0 40.0 54-55 31.279115011753255 35.0 29.5 39.0 9.0 40.0 56-57 30.813488568170488 35.0 28.5 38.0 8.0 40.0 58-59 30.35711962906451 34.5 28.0 38.0 7.0 40.0 60-61 29.806241715382818 34.0 27.5 38.0 3.5 40.0 62-63 29.186672682380028 34.0 26.0 38.0 2.0 39.5 64-65 28.52717273675834 33.0 26.0 37.0 2.0 39.0 66-67 27.87231756075027 32.5 24.5 36.5 2.0 39.0 68-69 27.20941977798572 32.0 23.0 36.0 2.0 39.0 70-71 26.642132803692128 31.0 22.0 35.5 2.0 38.0 72-73 25.907701961603525 31.0 20.5 35.0 2.0 38.0 74-75 25.212773735596475 30.5 19.0 34.5 2.0 37.0 76-77 24.53327468371741 29.5 19.5 33.5 2.0 36.0 78-79 25.02457872957179 30.5 19.5 34.0 2.0 36.0 80-81 24.66431511857165 30.5 18.0 34.0 2.0 36.0 82-83 24.0419808674472 30.0 14.0 34.0 2.0 35.5 84-85 23.27931045065087 29.5 7.5 34.0 2.0 35.0 86-87 22.46799270792168 29.0 2.0 34.0 2.0 35.0 88-89 21.565201754319844 29.0 2.0 33.5 2.0 35.0 90-91 20.484917824135145 26.5 2.0 33.0 2.0 34.5 92-93 19.48504623233647 25.5 2.0 32.0 2.0 34.0 94-95 18.431794785927107 25.0 2.0 32.0 2.0 34.0 96-97 17.239161486369348 21.5 2.0 32.0 2.0 34.0 98-99 15.732578748954161 6.5 2.0 31.0 2.0 34.0 100-101 13.370757010764752 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33983698229608805 1101 2 0.2719190397862583 1101 3 0.2528228878369525 1101 4 0.13749084284985003 1101 5 0.19780871380677922 1101 6 0.17176154020698675 1101 7 0.22613897940235006 1101 8 0.1160724912972455 1101 9 0.020047751675647874 1101 10-11 0.1735720719184357 1101 12-13 0.2898950839353702 1101 14-15 0.4075670434979273 1101 16-17 0.23463731659188625 1101 18-19 0.17895258297358652 1101 20-21 0.21877447090652424 1101 22-23 0.2924902396894282 1101 24-25 0.26889253899912546 1101 26-27 -0.010483204817973046 1101 28-29 -0.1750911760345062 1101 30-31 -0.15332240762379 1101 32-33 0.22260649618453243 1101 34-35 0.1694745808850442 1101 36-37 -0.08642964202929448 1101 38-39 -0.06585666905031928 1101 40-41 0.06950484297791348 1101 42-43 0.21851213560720595 1101 44-45 0.0012638586755215897 1101 46-47 -0.32114521120370654 1101 48-49 -0.2290588756710079 1101 50-51 -0.10199028994343706 1101 52-53 -0.16531265724173494 1101 54-55 -0.3540901405760053 1101 56-57 -0.30740941009042544 1101 58-59 -0.4618830592398169 1101 60-61 -0.40437531412308303 1101 62-63 -0.7256353961403939 1101 64-65 -0.7141573826946903 1101 66-67 -0.8943427536487683 1101 68-69 -0.6912003540689042 1101 70-71 -0.6268251011684427 1101 72-73 -0.7535943925938611 1101 74-75 -0.9225487619400923 1101 76-77 -0.659336670193543 1101 78-79 -0.695603524527634 1101 80-81 -0.6540556360545047 1101 82-83 -0.4456437098355899 1101 84-85 -0.620579862699401 1101 86-87 -0.6152021476519458 1101 88-89 -0.6315157816243122 1101 90-91 -0.5045171148111578 1101 92-93 -0.6138424978401922 1101 94-95 -0.7605194245318501 1101 96-97 -0.852545176396557 1101 98-99 -0.9753334637771864 1101 100-101 -1.01605148534159 1102 1 0.22806760972866158 1102 2 0.27138240695557414 1102 3 0.21522497373732108 1102 4 0.24948330309746325 1102 5 0.06668327618820769 1102 6 0.05985464783670835 1102 7 0.14899525959558702 1102 8 0.14206679003181932 1102 9 0.01164315252448489 1102 10-11 0.1742636437269951 1102 12-13 0.27308191283873384 1102 14-15 0.34030488552495086 1102 16-17 0.1352486990754116 1102 18-19 0.2513494247854666 1102 20-21 0.26929802407595815 1102 22-23 0.4207075918770329 1102 24-25 0.21374526445826092 1102 26-27 0.1172024041319304 1102 28-29 0.18996186382545233 1102 30-31 0.21885140762783095 1102 32-33 0.19627664913100062 1102 34-35 0.12155229118644684 1102 36-37 0.19272608417306003 1102 38-39 0.09602278047930213 1102 40-41 0.22493766974941565 1102 42-43 0.28573242644019814 1102 44-45 0.446194817622505 1102 46-47 0.1714360538317905 1102 48-49 0.07599213500786561 1102 50-51 0.20536524703166492 1102 52-53 0.2066319583384626 1102 54-55 0.15280785273503383 1102 56-57 0.3189822903144339 1102 58-59 0.21755980831088806 1102 60-61 0.24189410978506842 1102 62-63 -0.13144472157214437 1102 64-65 -0.03923952785692464 1102 66-67 -0.13057946617981742 1102 68-69 -0.0016592545950224746 1102 70-71 0.11295748307924569 1102 72-73 -0.11183347902230523 1102 74-75 -0.013719501637723397 1102 76-77 -0.040577013247389715 1102 78-79 0.19879135166787165 1102 80-81 0.1997620359124781 1102 82-83 0.4370213383757964 1102 84-85 0.2531256175564245 1102 86-87 -0.02577393665622907 1102 88-89 -0.22289921861701956 1102 90-91 -0.31316625473174753 1102 92-93 -0.31526469708132865 1102 94-95 -0.4959738021844551 1102 96-97 -0.5276393621815316 1102 98-99 -0.43903648701253317 1102 100-101 -0.2745413467705262 1103 1 0.3842238633212922 1103 2 0.3009682673986305 1103 3 0.3068137992156359 1103 4 0.22842141830121676 1103 5 0.316755279037082 1103 6 0.32266023934361954 1103 7 0.23354062419438293 1103 8 0.1776884720423979 1103 9 0.3279441568791057 1103 10-11 0.2415127022822361 1103 12-13 0.33902610752642204 1103 14-15 0.423717131577213 1103 16-17 0.15209786642605394 1103 18-19 0.2230799446600571 1103 20-21 0.16538849971135505 1103 22-23 0.15531400367564174 1103 24-25 0.14171798068611707 1103 26-27 0.21892059840062927 1103 28-29 0.3276393223071494 1103 30-31 0.32462389304600237 1103 32-33 0.3722892655522969 1103 34-35 0.5563197129717139 1103 36-37 0.5733477641807809 1103 38-39 0.48988998038764464 1103 40-41 0.42802465920515687 1103 42-43 0.5523757998347563 1103 44-45 0.6058378517324243 1103 46-47 0.6337318972144743 1103 48-49 0.44143323106948174 1103 50-51 0.22220239721699642 1103 52-53 0.1545196210759201 1103 54-55 0.058041087888124565 1103 56-57 0.26146302980271585 1103 58-59 0.31375531397117484 1103 60-61 0.2109248733277127 1103 62-63 0.27882180313174487 1103 64-65 0.18008455466254603 1103 66-67 -0.02851788141213163 1103 68-69 0.1613759248553066 1103 70-71 0.20525842574575037 1103 72-73 0.31476747778439673 1103 74-75 0.2771230378456764 1103 76-77 0.22361385699166192 1103 78-79 0.17214989701404448 1103 80-81 0.26173225417224444 1103 82-83 0.12420631339410804 1103 84-85 -0.05955591674717553 1103 86-87 0.14507860912015502 1103 88-89 -0.05440163667096698 1103 90-91 -0.1620364809628967 1103 92-93 -0.031620439623978314 1103 94-95 -0.23408051381134243 1103 96-97 -0.34665043760166014 1103 98-99 -0.2568962178520646 1103 100-101 -0.277721789715228 1104 1 0.31710212905483814 1104 2 0.21128295439934774 1104 3 0.17359180166523558 1104 4 0.20622351171397213 1104 5 0.14732932804840004 1104 6 0.24868235326616173 1104 7 0.16594271246361814 1104 8 0.17014236520505222 1104 9 0.13507580849561407 1104 10-11 0.22931211142034158 1104 12-13 0.28226856664348077 1104 14-15 0.18704387209626105 1104 16-17 0.1856744052226631 1104 18-19 0.2526139979790756 1104 20-21 0.07593484604386447 1104 22-23 0.13515622619075884 1104 24-25 0.14684864217498017 1104 26-27 0.17997289835787456 1104 28-29 0.13678016153678385 1104 30-31 0.21390801146269922 1104 32-33 0.29424458282712607 1104 34-35 0.39642253515700787 1104 36-37 0.28055307261519147 1104 38-39 0.13856162608911404 1104 40-41 0.21376529397985422 1104 42-43 0.3780321612442137 1104 44-45 0.37116915873307477 1104 46-47 0.25518045718953175 1104 48-49 0.11510560007847914 1104 50-51 0.2951821188882491 1104 52-53 0.498124859108394 1104 54-55 0.16620690855286213 1104 56-57 0.27505900146192275 1104 58-59 0.4613032210406196 1104 60-61 0.34140553529877593 1104 62-63 0.32230644972367983 1104 64-65 0.11697195335930743 1104 66-67 0.201754307252191 1104 68-69 0.3639380007157982 1104 70-71 0.16459467856263288 1104 72-73 0.3164740817429781 1104 74-75 0.1096299253408155 1104 76-77 0.01692752171772227 1104 78-79 0.3443308460578294 1104 80-81 0.23100132779209304 1104 82-83 0.259480093335025 1104 84-85 0.21045137258548507 1104 86-87 -0.11971805305664418 1104 88-89 -0.1195622029082557 1104 90-91 -0.06613012035711918 1104 92-93 -0.159783905520829 1104 94-95 -0.0167414658126539 1104 96-97 -0.1500594664085888 1104 98-99 -0.16430742783306584 1104 100-101 -0.22406844369454504 1105 1 0.31617380959574604 1105 2 0.2936246221802463 1105 3 0.29398074255156814 1105 4 0.18872864130316458 1105 5 0.2265643548477172 1105 6 0.25528994918494163 1105 7 0.15631121752883104 1105 8 0.09599958022191402 1105 9 0.28011030013393423 1105 10-11 0.2963190724750788 1105 12-13 0.2027556011219076 1105 14-15 0.19244737639388632 1105 16-17 0.14516189612311337 1105 18-19 0.21113664740570215 1105 20-21 -0.017183188980958164 1105 22-23 0.09059813018861718 1105 24-25 0.1895278146397672 1105 26-27 0.04215367118846558 1105 28-29 0.13596594758132596 1105 30-31 0.11957226643836094 1105 32-33 0.28151996211960295 1105 34-35 0.13648132528376777 1105 36-37 0.18975305066071257 1105 38-39 0.0042845185006257225 1105 40-41 0.2549012565681039 1105 42-43 0.18270829969937807 1105 44-45 0.08763142592071205 1105 46-47 0.06860722910563055 1105 48-49 -0.022785329916452213 1105 50-51 0.09652278633735278 1105 52-53 0.3571079954291889 1105 54-55 0.12073019102773941 1105 56-57 0.0011611203165244888 1105 58-59 0.0018288326708812974 1105 60-61 0.002337213957869011 1105 62-63 -0.23315264231839805 1105 64-65 0.10171854422525328 1105 66-67 -0.1315096961548825 1105 68-69 -0.1029469520490629 1105 70-71 -0.3466473959538341 1105 72-73 -0.2792406272611636 1105 74-75 -0.30597739655968326 1105 76-77 -0.20355423048061638 1105 78-79 -0.1367264023034238 1105 80-81 -0.2890585435513735 1105 82-83 -0.2640388461151879 1105 84-85 -0.09430926171731357 1105 86-87 -0.1917538066646891 1105 88-89 -0.13567511742280658 1105 90-91 0.008412859245026993 1105 92-93 0.001322645496916408 1105 94-95 -0.18421347385492481 1105 96-97 -0.31284324452641243 1105 98-99 -0.4951612877767104 1105 100-101 -0.4737688393703383 1106 1 0.13435140873511742 1106 2 0.10287258339714711 1106 3 0.039103754267298285 1106 4 -0.0443724164522763 1106 5 0.0443311160831783 1106 6 -0.07961750196909634 1106 7 0.11978468462794467 1106 8 -0.021589075174098582 1106 9 0.07021549746166045 1106 10-11 -0.09931136209251434 1106 12-13 -0.2663204776889785 1106 14-15 -0.10843851185668285 1106 16-17 -0.005848422755576621 1106 18-19 -0.02921760138099927 1106 20-21 0.2323772525024097 1106 22-23 0.013935777319943554 1106 24-25 0.03095275265270203 1106 26-27 0.05478693673354229 1106 28-29 0.037693901372712446 1106 30-31 0.03067949741370768 1106 32-33 0.19894801032762643 1106 34-35 -0.3663857907178354 1106 36-37 -0.005567657775998214 1106 38-39 0.0036572298862296293 1106 40-41 -0.1774356426860706 1106 42-43 -0.2116759265597068 1106 44-45 0.016127371178839667 1106 46-47 -0.2802245837470565 1106 48-49 -0.24041035207507377 1106 50-51 0.032348764530986784 1106 52-53 0.07484942166058062 1106 54-55 -0.16441984469233617 1106 56-57 -0.21570461253080353 1106 58-59 -0.1503691120744648 1106 60-61 -0.11110692387977039 1106 62-63 -0.5001326109273414 1106 64-65 -0.6840656274076089 1106 66-67 -0.8606967620646913 1106 68-69 -0.29544124786909265 1106 70-71 -0.3744626436700713 1106 72-73 -0.25838185431225114 1106 74-75 -0.7937861621667679 1106 76-77 -0.4827273355098036 1106 78-79 -0.6248741091812278 1106 80-81 -0.4915434281401616 1106 82-83 -0.6100579409479892 1106 84-85 -0.8758081657691648 1106 86-87 -0.5234498748698684 1106 88-89 -0.6104812437767677 1106 90-91 -0.5824816033447604 1106 92-93 -0.7624493638123688 1106 94-95 -0.595679046779793 1106 96-97 -0.43500323241430294 1106 98-99 -0.9412684845155699 1106 100-101 -1.1642378564863005 1107 1 0.2536852580825162 1107 2 0.20402813029650346 1107 3 0.15356681140997708 1107 4 0.034033458272716643 1107 5 0.2082075570188806 1107 6 0.045047746498390495 1107 7 0.1845934208244273 1107 8 0.1182762015006702 1107 9 0.019423930765718467 1107 10-11 0.09205695945700398 1107 12-13 0.2488053944839379 1107 14-15 0.18541344919265157 1107 16-17 0.11035688144367128 1107 18-19 0.27047533666989665 1107 20-21 0.21558133046867312 1107 22-23 0.26394529167907166 1107 24-25 0.23871697340150178 1107 26-27 0.15478774825241004 1107 28-29 0.2793011172865434 1107 30-31 0.244327090028996 1107 32-33 0.10981007842469381 1107 34-35 0.13244338344138384 1107 36-37 0.29432116413926934 1107 38-39 0.16295558507741248 1107 40-41 0.2270513850449234 1107 42-43 0.42006478700603367 1107 44-45 0.15234277179212796 1107 46-47 -0.09429833570099788 1107 48-49 -0.12821856833980405 1107 50-51 0.16653284947070546 1107 52-53 0.14648582986688297 1107 54-55 -0.13644930786420773 1107 56-57 -0.23251662502483228 1107 58-59 -0.2255977505193023 1107 60-61 -0.12183128562018197 1107 62-63 0.01660454594709293 1107 64-65 0.3832902986109765 1107 66-67 0.3638594630405656 1107 68-69 0.4277344157434655 1107 70-71 0.36001342016120574 1107 72-73 0.2746969139476221 1107 74-75 -0.1539817494070057 1107 76-77 0.15764570012482793 1107 78-79 0.27949916599138547 1107 80-81 0.20790902049209592 1107 82-83 0.1691845817451565 1107 84-85 0.3957711003155602 1107 86-87 0.06331940136163894 1107 88-89 0.15559676389404586 1107 90-91 0.18713140849278176 1107 92-93 0.023783134663936778 1107 94-95 0.21924204280461268 1107 96-97 0.21751768098656044 1107 98-99 0.2698219841673577 1107 100-101 0.042804532222280756 1108 1 0.15332914609791004 1108 2 0.024560596914671606 1108 3 -0.04651356312361443 1108 4 0.01713779263077697 1108 5 -0.06056253661451905 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0.516201883639372 2316 100-101 0.6447463413141552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 18.0 4 71.0 5 136.0 6 293.0 7 801.0 8 1706.0 9 2663.0 10 4374.0 11 6688.0 12 9858.0 13 13744.0 14 18159.0 15 24573.0 16 31591.0 17 39322.0 18 35022.0 19 28686.0 20 28756.0 21 29957.0 22 32980.0 23 36381.0 24 41576.0 25 48132.0 26 56881.0 27 66751.0 28 77885.0 29 89144.0 30 102349.0 31 116230.0 32 131490.0 33 148217.0 34 163983.0 35 174044.0 36 160930.0 37 105861.0 38 27421.0 39 676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.266479565215285 23.643384346479436 16.817537853312388 19.272598234992888 2 18.104479588749182 24.215040934488666 36.71855398905327 20.961925487708886 3 21.6203338290195 26.561869063476184 30.357690870580683 21.46010623692363 4 15.851279074901015 19.640855451668447 35.522715599254425 28.985149874176113 5 13.442604763184496 39.03708726329356 33.50913919397151 14.011168779550436 6 31.056263789748666 38.564886252407995 16.220674743372037 14.158175214471308 7 27.45938047484653 34.561027007179014 20.35843386143113 17.621158656543326 8 26.450474275826203 36.388784499272624 18.964303058430307 18.19643816647087 9 26.378436467283095 17.042917951197346 20.472359125165962 36.106286456353594 10-11 25.027727557099922 28.32141138111231 26.400483913171293 20.250377148616476 12-13 25.88470709157482 25.51362989895792 27.935619291598996 20.666043717868266 14-15 22.69212505074412 26.573983044733435 26.77849396831413 23.955397936208314 16-17 21.164309734579977 30.092771560463845 27.422150172449257 21.320768532506925 18-19 21.16229073770377 27.702440778783625 31.06145934171011 20.073809141802496 20-21 21.862828813831257 26.678270963379163 31.535573648160454 19.923326574629126 22-23 21.607597710500286 26.75140751684255 31.46740327208295 20.173591500574204 24-25 21.37947891987174 26.91337432535056 31.636357860794945 20.070788893982755 26-27 21.268838126845047 27.16386417758328 31.365821065320944 20.20147663025073 28-29 21.137173750761505 27.196506404449583 31.499780734991383 20.166539109797522 30-31 21.205012711604333 27.06231108919228 31.501708340556856 20.23096785864653 32-33 21.170232125416856 27.10231414729955 31.354402328468716 20.373051398814876 34-35 21.54059691238846 27.111843812555254 31.269873659251473 20.077685615804818 36-37 21.65686421249969 26.905663851556888 31.155598436058103 20.28187349988532 38-39 21.361794549031472 27.26453381631328 31.064312857295146 20.3093587773601 40-41 21.53278511337019 27.151021256422815 31.020847236471212 20.295346393735787 42-43 21.64374717645773 27.19837837808732 30.69413236281556 20.463742082639396 44-45 21.667901395422962 27.4681294613101 30.32145660664234 20.542512536624603 46-47 21.872654598628806 27.754450138314745 29.69648285360173 20.67641240945472 48-49 21.96477469610059 27.911285815658587 29.369647639281173 20.754291848959653 50-51 22.066882098120015 28.157764954306064 28.95653395845066 20.818818989123262 52-53 22.333955004904816 28.279012446711942 28.67820850907579 20.708824039307448 54-55 22.07560695838788 28.394483447029927 28.72309582091312 20.80681377366907 56-57 22.071808450497965 28.550096427290804 28.61919996037382 20.75889516183741 58-59 22.22016434096173 28.21235862983873 28.751296195994524 20.816180833205014 60-61 22.135366472160996 28.21903477950939 28.972685933460323 20.672912814869292 62-63 22.004616234457764 28.263533470661013 29.08588435831972 20.645965936561502 64-65 21.972635323938626 28.183581194363178 28.912923625924563 20.930859855773633 66-67 21.989218018281882 28.408739725997894 28.68036210574375 20.92168014997647 68-69 22.051322362194043 28.347523740711267 28.655703423895662 20.945450473199028 70-71 22.111246189479896 28.435255884972094 28.41439291725128 21.039105008296733 72-73 22.22511761329803 28.49458747317426 28.176662765067366 21.103632148460342 74-75 22.171014465443513 28.753269093322096 27.898829385296047 21.176887055938344 76-77 22.064540061743617 28.514265962727702 28.353688411173287 21.06750556435539 78-79 22.108984912978542 28.56296416738184 28.150523485510064 21.177527434129555 80-81 21.999770641954864 28.775308798842225 28.09864872577069 21.12627183343222 82-83 22.223302016313564 28.670740571243975 28.021078418176433 21.084878994266024 84-85 22.086216573815218 28.646800629388796 27.986103913759198 21.280878883036788 86-87 22.22134881912911 28.41724643283632 28.135609828584474 21.2257949194501 88-89 22.241484947974488 28.395845065948517 28.01401237672005 21.34865760935695 90-91 22.210531558733692 28.535120676634545 27.972748309557883 21.281599455073884 92-93 22.225697945031232 28.384439470152678 27.921166299427536 21.46869628538855 94-95 22.116307141649592 28.253519246155022 27.92654943204272 21.70362418015267 96-97 22.26590135019743 28.2714210184574 27.811708889724006 21.650968741621163 98-99 22.171525330648485 28.301006549706976 27.615212118438514 21.91225600120603 100-101 22.619314302207613 27.94007413996989 27.94723612137243 21.49337543645007 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1066.0 1 742.5 2 488.5 3 576.0 4 528.5 5 539.5 6 972.0 7 1992.5 8 3043.0 9 3416.0 10 3127.5 11 2854.0 12 2998.0 13 3278.0 14 3682.0 15 4024.5 16 4294.5 17 4601.5 18 4858.0 19 5197.0 20 5591.0 21 6119.0 22 6965.5 23 8059.0 24 9226.5 25 10841.5 26 13346.5 27 16095.5 28 18410.5 29 21525.0 30 25451.0 31 29034.0 32 33161.5 33 37815.0 34 42508.0 35 46306.5 36 51210.5 37 55972.0 38 58151.5 39 59702.5 40 61685.5 41 64644.5 42 67047.5 43 68628.5 44 69995.5 45 71393.0 46 70720.5 47 69356.5 48 69146.5 49 67403.5 50 64499.5 51 60599.0 52 56250.5 53 52680.0 54 49559.0 55 45274.0 56 39976.0 57 35226.5 58 31506.0 59 28089.5 60 23803.5 61 19571.0 62 15732.5 63 12056.0 64 9075.0 65 6907.0 66 5180.0 67 3929.5 68 2869.0 69 1923.5 70 1356.5 71 1013.5 72 783.0 73 596.5 74 461.0 75 371.0 76 266.5 77 177.0 78 121.0 79 83.5 80 52.5 81 37.0 82 29.5 83 17.5 84 10.5 85 8.5 86 4.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013729178758214625 2 0.0 3 0.0 4 0.0 5 0.03095795210185651 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003364994793680055 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.8455925028992796E-4 24-25 2.4227962514496398E-4 26-27 0.0011306382506764987 28-29 8.883586255315345E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.26838875434892E-4 42-43 0.011037182923270581 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.345997917472022E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.6919958349440442E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.307193335910471E-4 84-85 2.6919958349440442E-5 86-87 0.0 88-89 0.0 90-91 0.01214090121559764 92-93 0.001642117459315867 94-95 0.0 96-97 0.0 98-99 0.0012383180840742603 100-101 0.01776717251063069 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.07449043581855 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28622577156906 43.337857278483625 2 12.619534201862983 14.15267899813702 3 4.209090554532518 7.0806782413088385 4 1.9089053969957253 4.281635897068768 5 1.060684478296889 2.9738670816840043 6 0.6346779638176473 2.1353546047150473 7 0.4356842763224699 1.7101541649976606 8 0.296599616791752 1.3305337900045235 9 0.21054843567434375 1.0625756618247792 >10 1.2129488666798656 12.504712255341122 >50 0.08536988193007991 3.278003474674275 >100 0.037523814588525384 3.64084933004781 >500 0.0015351241309143862 0.5617343054784364 >1k 3.837810327285965E-4 0.37838112924335243 >5k 1.9189051636429825E-4 0.9005180351592824 >10k+ 9.594525818214913E-5 0.67046575183146 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12462 0.6709530419014536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9368 0.5043723396351161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7370 0.3968001860707521 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.1366457085817597 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.10380335939544234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.3839916698880884E-5 0.0 16-17 0.0 0.0 0.0 5.3839916698880884E-5 0.0 18-19 0.0 0.0 0.0 5.3839916698880884E-5 0.0 20-21 0.0 0.0 0.0 1.0767983339776177E-4 0.0 22-23 0.0 0.0 0.0 2.4227962514496398E-4 0.0 24-25 0.0 0.0 0.0 3.768794168921662E-4 0.0 26-27 0.0 0.0 0.0 9.691185005798559E-4 0.0 28-29 0.0 0.0 0.0 0.0018574771261113904 0.0 30-31 0.0 0.0 0.0 0.0035803544604755787 0.0 32-33 0.0 0.0 0.0 0.006003150711925219 0.0 34-35 0.0 0.0 0.0 0.00926046567220751 0.0 36-37 0.0 0.0 0.0 0.013890698508311268 0.0 38-39 0.0 0.0 0.0 0.02199360597149284 0.0 40-41 0.0 0.0 0.0 0.03233086997767797 0.0 42-43 0.0 0.0 0.0 0.04086449677445059 0.0 44-45 0.0 0.0 0.0 0.054432155782568575 0.0 46-47 0.0 0.0 0.0 0.07211856841815095 0.0 48-49 0.0 0.0 0.0 0.08743602471898256 0.0 50-51 0.0 0.0 0.0 0.10749139368931568 0.0 52-53 0.0 0.0 0.0 0.12751984270129937 0.0 54-55 0.0 0.0 0.0 0.1487866097973573 0.0 56-57 0.0 0.0 0.0 0.1811982396500836 0.0 58-59 0.0 0.0 0.0 0.21673258467134499 0.0 60-61 0.0 0.0 0.0 0.2522400097342569 0.0 62-63 0.0 0.0 0.0 0.2900087112985219 0.0 64-65 0.0 0.0 0.0 0.3310347278230691 0.0 66-67 0.0 0.0 0.0 0.38226340856205426 0.0 68-69 0.0 0.0 0.0 0.441594996764221 0.0 70-71 0.0 0.0 0.0 0.512313727348201 0.0 72-73 0.0 0.0 0.0 0.5904085265199277 0.0 74-75 0.0 0.0 0.0 0.6782214306558025 0.0 76-77 0.0 0.0 0.0 0.7798981133416389 2.6919958349440442E-5 78-79 0.0 0.0 0.0 0.8934734176179282 5.3839916698880884E-5 80-81 0.0 0.0 0.0 1.0187319838178746 5.3839916698880884E-5 82-83 0.0 0.0 0.0 1.156642930442058 5.3839916698880884E-5 84-85 0.0 0.0 0.0 1.300449347944769 5.3839916698880884E-5 86-87 0.0 0.0 0.0 1.4533277914112412 5.3839916698880884E-5 88-89 0.0 0.0 0.0 1.6179971766347685 5.3839916698880884E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5565 0.0 55.073586 1 GTATCAA 10020 0.0 41.68406 1 TATCAAC 13610 0.0 30.648912 2 ATCAACG 13910 0.0 30.05621 3 TCAACGC 14325 0.0 29.218634 4 CAACGCA 14590 0.0 28.65537 5 AACGCAG 15045 0.0 27.813595 6 TGGTATC 2465 0.0 25.248352 2 ACGCAGA 17620 0.0 23.560198 7 CGCAGAG 17660 0.0 23.479939 8 TGCACCG 290 3.6379788E-12 22.935526 5 GTGGTAT 2950 0.0 22.872581 1 GCAGAGT 18450 0.0 22.036917 9 GAGTACT 11385 0.0 21.235289 12-13 AGTACTT 11610 0.0 21.089674 12-13 GTACTTT 12070 0.0 19.872728 14-15 AGAGTAC 17435 0.0 19.805502 10-11 CAGAGTA 18135 0.0 19.263649 10-11 TACTTTT 12965 0.0 18.885548 14-15 GTACATG 9645 0.0 18.031326 1 >>END_MODULE