FastQCFastQC Report
Thu 26 May 2016
SRR937826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122970.6622056504769044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79390.4275230266842437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62310.3355455320908832No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23170.12477274881312413No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20580.11082534184609819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.056.2429961
GTATCAA81850.048.620271
ATCAACG107300.036.8497733
TATCAAC108500.036.5308072
TCAACGC109350.036.1648064
CAACGCA112000.035.3496485
AACGCAG116200.034.0953676
GTGTACG1751.5097612E-1029.8858221
ACGCAGA133900.029.4811337
CGCAGAG135150.029.138958
GTGGTAT18150.028.815531
GTACATG90550.027.4089661
GCAGAGT144650.026.995349
TACATGG90900.026.5073932
TGGTATC18450.026.2740352
ACATGGG89650.025.285953
GAGTACT88300.023.83170112-13
AATACCG1050.002188963622.6328285
AGTACTT93400.022.50496912-13
AGAGTAC135850.022.25618710-11
Produced by FastQC (version 0.11.5)