##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937826_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856976 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19364385969447 33.0 31.0 34.0 31.0 34.0 2 32.34519024478507 34.0 31.0 34.0 31.0 34.0 3 32.40102618450642 34.0 31.0 34.0 31.0 34.0 4 35.97020478455295 37.0 35.0 37.0 35.0 37.0 5 35.961460460447526 37.0 35.0 37.0 35.0 37.0 6 36.05789412464135 37.0 37.0 37.0 35.0 37.0 7 36.01494634287142 37.0 37.0 37.0 35.0 37.0 8 36.00903942754241 37.0 37.0 37.0 35.0 37.0 9 37.70898654586812 39.0 38.0 39.0 35.0 39.0 10-11 37.617982946467805 39.0 38.0 39.0 35.0 39.0 12-13 37.51931635088445 39.0 37.0 39.0 35.0 39.0 14-15 38.76998948828633 40.0 38.0 41.0 35.5 41.0 16-17 38.721761885990986 40.0 38.0 41.0 36.0 41.0 18-19 38.683230693342296 40.0 38.0 41.0 35.0 41.0 20-21 38.52212225683046 40.0 38.0 41.0 35.0 41.0 22-23 38.398092920963975 40.0 38.0 41.0 34.0 41.0 24-25 38.269744466272044 40.0 38.0 41.0 34.0 41.0 26-27 38.36428176777729 40.0 38.0 41.0 34.0 41.0 28-29 38.303191586751794 40.0 38.0 41.0 34.0 41.0 30-31 38.08831104979278 40.0 38.0 41.0 34.0 41.0 32-33 37.8472241967586 40.0 37.5 41.0 34.0 41.0 34-35 37.5995979484926 40.0 37.0 41.0 33.0 41.0 36-37 37.632663534692966 40.0 37.0 41.0 33.0 41.0 38-39 37.595444690722985 40.0 37.0 41.0 33.0 41.0 40-41 37.407629393163944 40.0 37.0 41.0 33.0 41.0 42-43 37.15004878899889 40.0 37.0 41.0 32.5 41.0 44-45 36.88841805171418 40.0 37.0 41.0 31.5 41.0 46-47 36.492339696366564 39.5 36.0 41.0 30.5 41.0 48-49 36.13832650502753 39.0 36.0 41.0 30.0 41.0 50-51 35.2568406915329 38.5 35.0 40.0 28.5 40.5 52-53 35.36622282678936 38.5 35.0 40.0 29.0 40.5 54-55 35.67657874953689 39.0 35.0 40.0 29.0 41.0 56-57 35.43773640585554 39.0 35.0 40.0 28.0 41.0 58-59 35.22532062880727 38.5 35.0 40.0 27.5 41.0 60-61 34.939578648297015 38.0 35.0 40.0 26.5 41.0 62-63 34.591136342095965 38.0 34.0 40.0 26.0 41.0 64-65 34.235044502459914 37.5 34.0 40.0 26.0 41.0 66-67 33.83862930915639 37.0 34.0 39.5 25.0 41.0 68-69 33.37059741213672 36.0 33.0 39.0 23.0 40.5 70-71 32.94177630728669 36.0 33.0 39.0 22.0 40.0 72-73 32.483109097802014 35.0 32.5 38.0 21.0 40.0 74-75 31.993284242768887 35.0 32.0 37.5 19.5 39.5 76-77 31.538089614513055 35.0 31.5 37.0 18.0 39.0 78-79 31.017897377241276 35.0 31.0 36.0 14.5 39.0 80-81 30.500726719139074 34.0 30.5 36.0 7.5 37.5 82-83 30.000306142890377 34.0 30.0 35.0 6.5 37.0 84-85 29.549338279008452 34.0 29.5 35.0 2.0 36.5 86-87 29.04063111208761 34.0 29.0 35.0 2.0 36.0 88-89 28.570978569459164 34.0 29.0 35.0 2.0 36.0 90-91 28.086384530548592 33.5 28.0 35.0 2.0 35.0 92-93 27.555752470683522 33.0 26.5 35.0 2.0 35.0 94-95 26.94290260078752 33.0 25.5 35.0 2.0 35.0 96-97 26.19083741523854 32.0 24.5 34.0 2.0 35.0 98-99 25.26611033206676 32.0 21.0 34.0 2.0 35.0 100-101 22.652170518089626 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2510678017057586 1101 2 0.3126846344784511 1101 3 0.2805393494883859 1101 4 0.18805405600807035 1101 5 0.1588156784087431 1101 6 0.2207828667573537 1101 7 0.21866015407991313 1101 8 0.24632020701702828 1101 9 0.37192912007112966 1101 10-11 0.3348350575444101 1101 12-13 0.3420417437336809 1101 14-15 0.5021975027305743 1101 16-17 0.5693737771889076 1101 18-19 0.5232076897105387 1101 20-21 0.6263406974593622 1101 22-23 0.6112370086403729 1101 24-25 0.6244126838836266 1101 26-27 0.6904760859471182 1101 28-29 0.542575784717549 1101 30-31 0.5497442690792056 1101 32-33 0.5821375484255569 1101 34-35 0.6532839115630864 1101 36-37 0.6968582816998037 1101 38-39 0.6978711601836451 1101 40-41 0.7547586895678933 1101 42-43 0.8298206502804888 1101 44-45 1.0433699672190855 1101 46-47 1.2320205570320155 1101 48-49 1.299630310970315 1101 50-51 1.0402697394049838 1101 52-53 0.9909891385137044 1101 54-55 1.2233949942086255 1101 56-57 1.2551973575577833 1101 58-59 1.234631845633416 1101 60-61 1.3015569066800978 1101 62-63 1.318302753815999 1101 64-65 1.2993204063193033 1101 66-67 1.3067970575514352 1101 68-69 1.4039952420119661 1101 70-71 1.5831766235752056 1101 72-73 1.5009326506875666 1101 74-75 1.403738443323391 1101 76-77 1.440272451194641 1101 78-79 1.5592814043976375 1101 80-81 1.621461514299579 1101 82-83 1.6954807717180564 1101 84-85 1.7208826890290716 1101 86-87 1.9087150989491057 1101 88-89 1.8891082326229416 1101 90-91 1.7361170913255428 1101 92-93 1.8744431999651532 1101 94-95 1.9244970374374617 1101 96-97 1.9452246707372893 1101 98-99 2.23099179954518 1101 100-101 2.3696087556471 1102 1 0.19412018905724437 1102 2 0.21980630357415976 1102 3 0.21486015655644763 1102 4 0.10904308006708874 1102 5 0.13059971705087037 1102 6 0.17595671905878874 1102 7 0.1446365477297391 1102 8 0.12138193891861704 1102 9 0.14688221092664122 1102 10-11 0.10323888625369193 1102 12-13 0.2333644839732969 1102 14-15 0.34153665628900853 1102 16-17 0.2806426229487968 1102 18-19 0.29488663766756673 1102 20-21 0.3045962453421893 1102 22-23 0.3003165420519309 1102 24-25 0.21499188977438877 1102 26-27 0.2221229386408723 1102 28-29 0.2777558679025063 1102 30-31 0.26711281252585906 1102 32-33 0.21128033151802583 1102 34-35 0.37892097946196657 1102 36-37 0.3965840163138665 1102 38-39 0.48636763556277884 1102 40-41 0.42217995587515134 1102 42-43 0.5346646740466028 1102 44-45 0.38956717557024945 1102 46-47 0.45049619179218325 1102 48-49 0.5816442709897061 1102 50-51 0.6286043183232906 1102 52-53 0.4684395532846253 1102 54-55 0.535703119387243 1102 56-57 0.8033177964534062 1102 58-59 0.6487926379319688 1102 60-61 0.5848990567431969 1102 62-63 0.5801251008531381 1102 64-65 0.6304142015309324 1102 66-67 0.6320518403314779 1102 68-69 0.6108587111051804 1102 70-71 0.6550085033315796 1102 72-73 0.557471261750841 1102 74-75 0.5726214820063475 1102 76-77 0.6279068989271117 1102 78-79 0.6617475738954219 1102 80-81 0.813751905425093 1102 82-83 0.9071121093343066 1102 84-85 0.9006202463452126 1102 86-87 0.7889550323443331 1102 88-89 0.9184059002132976 1102 90-91 1.0436772300026398 1102 92-93 1.0503798295719697 1102 94-95 1.1004199093954696 1102 96-97 1.4158477413379913 1102 98-99 1.499512080094707 1102 100-101 1.4055629062853647 1103 1 0.19211507669614036 1103 2 0.24947254536528618 1103 3 0.16940620937032236 1103 4 0.14299663932337126 1103 5 0.18308904898027123 1103 6 0.1613691716066583 1103 7 0.22306176039841574 1103 8 0.11859161499720017 1103 9 0.09406106043756779 1103 10-11 0.2729323870434115 1103 12-13 0.2128201352797916 1103 14-15 0.33374619382122006 1103 16-17 0.35206016014849695 1103 18-19 0.25115707325736025 1103 20-21 0.23370968711115125 1103 22-23 0.2943922992078569 1103 24-25 0.2752451871780295 1103 26-27 0.14095173688964735 1103 28-29 0.18196525303371658 1103 30-31 0.25779712769114127 1103 32-33 0.2505655015144228 1103 34-35 0.0783551907174811 1103 36-37 0.25787606513760863 1103 38-39 0.3866114771765723 1103 40-41 0.4051647296289218 1103 42-43 0.4545704971505984 1103 44-45 0.3149654963882327 1103 46-47 0.4386135890600542 1103 48-49 0.33408857534836756 1103 50-51 0.23480063160435094 1103 52-53 0.2772033040119979 1103 54-55 0.2518990170291886 1103 56-57 0.5248763045860798 1103 58-59 0.512430276824702 1103 60-61 0.5061472210037792 1103 62-63 0.4576147791484573 1103 64-65 0.575823467512258 1103 66-67 0.5704394030237054 1103 68-69 0.5384689247436896 1103 70-71 0.5598662854812702 1103 72-73 0.5126350581985051 1103 74-75 0.6277212007572075 1103 76-77 0.497696862627965 1103 78-79 0.4737186731223346 1103 80-81 0.6067786933660173 1103 82-83 0.5139653055990969 1103 84-85 0.6838291188548418 1103 86-87 0.6169121314604062 1103 88-89 0.7167379750837561 1103 90-91 0.8615630470105948 1103 92-93 1.042518708454729 1103 94-95 0.9410895040087865 1103 96-97 0.7687315840601521 1103 98-99 1.1421590777948367 1103 100-101 1.5568565830289707 1104 1 0.20669255106790274 1104 2 0.26376085163399665 1104 3 0.23103020011694042 1104 4 0.13558883291858592 1104 5 0.1898731594765053 1104 6 0.2302762295763543 1104 7 0.1713148448581805 1104 8 0.2222782248130457 1104 9 0.27055282765157784 1104 10-11 0.29841319988927495 1104 12-13 0.34710753435444985 1104 14-15 0.41698408278066523 1104 16-17 0.374517465328573 1104 18-19 0.41075093382163885 1104 20-21 0.5039202695297362 1104 22-23 0.4796484114277604 1104 24-25 0.45182200383410986 1104 26-27 0.49579785409911636 1104 28-29 0.3697643435716813 1104 30-31 0.45787877515554243 1104 32-33 0.5396967498134373 1104 34-35 0.4263066331560168 1104 36-37 0.5312717491619239 1104 38-39 0.5454526501784756 1104 40-41 0.6209068143608505 1104 42-43 0.5694283511830704 1104 44-45 0.6463478900197259 1104 46-47 0.5055418300107917 1104 48-49 0.512247311627057 1104 50-51 0.5564264367390663 1104 52-53 0.5390804585978586 1104 54-55 0.5081118787728158 1104 56-57 0.666407081144186 1104 58-59 0.7078412924284407 1104 60-61 0.6730227313004278 1104 62-63 0.6657775411007378 1104 64-65 0.5697438498945573 1104 66-67 0.6112603002386194 1104 68-69 0.7290136947699963 1104 70-71 0.7017457647518839 1104 72-73 0.5801515577902734 1104 74-75 0.46094916728175406 1104 76-77 0.4156723457087921 1104 78-79 0.38654016001337865 1104 80-81 0.5695071928670608 1104 82-83 0.5694458916464491 1104 84-85 0.5706254764707346 1104 86-87 0.626553903210386 1104 88-89 0.7116817857523579 1104 90-91 0.8995673902021046 1104 92-93 0.9235784812026218 1104 94-95 0.9790895947281939 1104 96-97 1.034284292034389 1104 98-99 1.136411153001223 1104 100-101 1.4616848398556037 1105 1 0.15843303382693819 1105 2 0.20007696882610304 1105 3 0.21392869827278105 1105 4 0.16575847934019805 1105 5 0.2320134661613622 1105 6 0.18263453604671298 1105 7 0.19567456498664626 1105 8 0.28519010728725647 1105 9 0.2489507835823872 1105 10-11 0.37860720174833773 1105 12-13 0.31001651205172465 1105 14-15 0.2767807409465206 1105 16-17 0.3354896457584218 1105 18-19 0.300335614028036 1105 20-21 0.4553864743120428 1105 22-23 0.42704355270419114 1105 24-25 0.3761480955833534 1105 26-27 0.3558261496057966 1105 28-29 0.3094024269849527 1105 30-31 0.3462878586164848 1105 32-33 0.3597381838496716 1105 34-35 0.45899244950260965 1105 36-37 0.44319751572511734 1105 38-39 0.5903424399620008 1105 40-41 0.7010537066964062 1105 42-43 0.45322768397815594 1105 44-45 0.4787719598952478 1105 46-47 0.5701044078439139 1105 48-49 0.6369208966282827 1105 50-51 0.5699936070990219 1105 52-53 0.5165446963248002 1105 54-55 0.5974027668599504 1105 56-57 0.7905954194330178 1105 58-59 0.8394538495557242 1105 60-61 0.8152929336516621 1105 62-63 0.7972618039130452 1105 64-65 0.8693742915100202 1105 66-67 0.9031456941263727 1105 68-69 0.9498661201842609 1105 70-71 0.9654794011751022 1105 72-73 0.8810401774700622 1105 74-75 0.9661955482276028 1105 76-77 0.7642949228268954 1105 78-79 0.8845498291965264 1105 80-81 0.7928364992361203 1105 82-83 0.9684211012372792 1105 84-85 1.0516362420209724 1105 86-87 1.148043666478511 1105 88-89 1.123627289902462 1105 90-91 1.0689958604422891 1105 92-93 1.1144179505669065 1105 94-95 1.379366367833832 1105 96-97 1.193800862669626 1105 98-99 1.5214898910767403 1105 100-101 1.5026314880409402 1106 1 0.034196270733389156 1106 2 0.11923902104551587 1106 3 0.11876845110457879 1106 4 0.06150496087507662 1106 5 0.11589189889469509 1106 6 -0.007458494142284167 1106 7 0.0329480986676316 1106 8 -0.03508735417212705 1106 9 0.1155950907473624 1106 10-11 -0.0018423667825189227 1106 12-13 -0.1753194832434204 1106 14-15 -0.09202488289579236 1106 16-17 -0.0012352859412416706 1106 18-19 -0.13632447918949708 1106 20-21 -0.016879394417728122 1106 22-23 -0.030793106452293273 1106 24-25 -0.09603662322452777 1106 26-27 -0.09653803169432962 1106 28-29 0.053779562134089076 1106 30-31 0.2330394262252682 1106 32-33 0.24959971784996782 1106 34-35 0.035799476006559416 1106 36-37 0.2425591702793639 1106 38-39 0.2752750682175673 1106 40-41 0.2068535333593573 1106 42-43 0.31766872494532805 1106 44-45 0.06877282332227708 1106 46-47 0.28526869112617703 1106 48-49 0.5670594685199788 1106 50-51 0.5046333014458568 1106 52-53 0.5430542477826137 1106 54-55 0.4933767125422506 1106 56-57 0.5378236965051215 1106 58-59 0.5960821792480004 1106 60-61 0.4665283877572577 1106 62-63 0.5784896582317245 1106 64-65 0.5395608487658237 1106 66-67 0.8260581556590765 1106 68-69 0.8869680070166694 1106 70-71 0.6525660151238455 1106 72-73 0.7776233075722203 1106 74-75 0.7974177617612526 1106 76-77 0.973290284757578 1106 78-79 0.9021964244431189 1106 80-81 0.7605793543775015 1106 82-83 0.7099434289713713 1106 84-85 0.7685421431373989 1106 86-87 0.5921373776344794 1106 88-89 0.9284236473808001 1106 90-91 1.1292238662679814 1106 92-93 1.0466360782987145 1106 94-95 1.0619643830920538 1106 96-97 1.2374389531600372 1106 98-99 1.6174657102327643 1106 100-101 1.383694517399089 1107 1 0.21193344717207196 1107 2 0.26360280395074653 1107 3 0.2966939916546707 1107 4 0.18450218960688147 1107 5 0.1931011305983077 1107 6 0.20324789276190103 1107 7 0.23446690070053222 1107 8 0.18218432178620958 1107 9 0.17016744453002275 1107 10-11 0.28903097422862345 1107 12-13 0.35344603232515226 1107 14-15 0.4375636811823753 1107 16-17 0.3102251303792585 1107 18-19 0.39125856813597437 1107 20-21 0.3604716952770062 1107 22-23 0.40226147664469636 1107 24-25 0.32772139120287136 1107 26-27 0.36378983645365537 1107 28-29 0.2863469291619154 1107 30-31 0.21948938583083333 1107 32-33 0.2596923576595813 1107 34-35 0.30765866559682564 1107 36-37 0.29954361126051765 1107 38-39 0.31511801695388186 1107 40-41 0.36202951175454245 1107 42-43 0.2921222302844555 1107 44-45 0.2659931515580851 1107 46-47 0.41566094207969684 1107 48-49 0.396692843680178 1107 50-51 0.25651873309331563 1107 52-53 0.22873625955907073 1107 54-55 0.2983582628863175 1107 56-57 0.37498614515262574 1107 58-59 0.3383383611241726 1107 60-61 0.43248354420690305 1107 62-63 0.3057760261132785 1107 64-65 0.23620462525378372 1107 66-67 0.3739530136291904 1107 68-69 0.44614501580413446 1107 70-71 0.40182103386953827 1107 72-73 0.3300874873551436 1107 74-75 0.34710124320750424 1107 76-77 0.43907104325617397 1107 78-79 0.4694141217885459 1107 80-81 0.5281986802965939 1107 82-83 0.4769255637455352 1107 84-85 0.5032013306267764 1107 86-87 0.5707338191045679 1107 88-89 0.6051933389506843 1107 90-91 0.5717495353544955 1107 92-93 0.7378483314677204 1107 94-95 0.8250743103820746 1107 96-97 0.7629351935174178 1107 98-99 1.0062790784027165 1107 100-101 0.953410366914067 1108 1 0.17565626270084778 1108 2 0.1664638535246752 1108 3 0.1801319084042987 1108 4 0.230347428826569 1108 5 0.2250648541987701 1108 6 0.12718200141579672 1108 7 0.22918457257242864 1108 8 0.18936487620434406 1108 9 0.17850812787578718 1108 10-11 0.29623808549973774 1108 12-13 0.2693217713709757 1108 14-15 0.3653535047533012 1108 16-17 0.3657110305218296 1108 18-19 0.3265089050678682 1108 20-21 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-0.19107995320329252 2316 74-75 -0.16756567478464746 2316 76-77 -0.27642541714968516 2316 78-79 -0.10854278149994911 2316 80-81 0.05964858714347443 2316 82-83 -0.22295449565255154 2316 84-85 -0.3315678678637006 2316 86-87 -0.14645516139470516 2316 88-89 -0.00538202251203046 2316 90-91 -0.22324628243228517 2316 92-93 0.011509812863184976 2316 94-95 0.2542866349801649 2316 96-97 0.13835488745009172 2316 98-99 0.10541239815998438 2316 100-101 0.8287964011829736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6727.0 3 965.0 4 850.0 5 853.0 6 1011.0 7 1104.0 8 1311.0 9 1677.0 10 2168.0 11 2920.0 12 4496.0 13 6580.0 14 7740.0 15 10427.0 16 14585.0 17 20920.0 18 16858.0 19 9782.0 20 9051.0 21 9100.0 22 9726.0 23 10454.0 24 11711.0 25 13387.0 26 15760.0 27 18553.0 28 22910.0 29 28338.0 30 35938.0 31 47644.0 32 63353.0 33 89284.0 34 131613.0 35 199641.0 36 303260.0 37 412066.0 38 288322.0 39 25889.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60692803129722 19.09394026784528 14.992174614644327 23.306957086213174 2 14.520155260558095 19.846844852810182 41.87440954021065 23.758590346421073 3 18.590822890895716 24.886353637358383 29.77909878609868 26.743724685647223 4 12.138461513544854 16.383176404598107 37.70646327565274 33.771898806204305 5 13.28542892619986 37.29228526696539 34.13464341629326 15.287642390541489 6 30.972383190781734 36.31984866437462 17.913210788598462 14.794557356245184 7 27.80189389770449 33.22381269921426 20.959821573083172 18.01447182999808 8 25.781608680710473 34.689285226812316 20.102331648846206 19.426774443631004 9 26.051056762649665 16.95449696738175 20.886460132441325 36.107986137527256 10-11 24.106074541068757 27.077664955035623 27.46478512254219 21.35147538135343 12-13 25.12522280040995 24.384960260796866 28.560888077645558 21.92892886114763 14-15 22.146296482146344 25.44905268633056 27.275883725974538 25.12876710554856 16-17 20.965788027711397 29.016476267468995 27.77379005403253 22.243945650787076 18-19 20.811893265757387 27.031007766575964 31.066142007138314 21.090956960528334 20-21 21.585382399247347 26.17156238797806 31.181055510016265 21.06199970275833 22-23 21.202534377238944 26.09848550242993 31.193999413018897 21.504980707312228 24-25 20.874360749205437 26.245901754321604 31.437786243274346 21.441951253198617 26-27 20.709725417247526 26.628204076876266 31.09912946650151 21.5629410393747 28-29 20.60508730219322 26.656041255658124 31.363208039630553 21.375663402518104 30-31 20.769355897308074 26.432362163070604 31.28543492786968 21.512847011751642 32-33 20.618421535386933 26.500003237300607 31.16689579560979 21.71467943170267 34-35 21.00903039581324 26.647207591197557 30.985007789475105 21.358754223514097 36-37 21.03420995205962 26.43704483543742 30.850672710936465 21.678072501566497 38-39 20.780477568963438 26.652386293970874 30.972288938581897 21.594847198483784 40-41 21.026594957080246 26.55169614424771 30.91856937918669 21.503139519485355 42-43 20.9318961417713 26.561537799646807 30.723166014938542 21.78340004364335 44-45 20.82300583950151 26.836729436789803 30.556650231689215 21.783614492019474 46-47 21.032974935337577 27.00654460041469 30.035597037329552 21.92488342691818 48-49 21.174819605900804 27.140153293170222 29.737186272891194 21.947840828037783 50-51 21.266913153562992 27.374369642942888 29.4299257679876 21.928791435506522 52-53 21.306519187244422 27.425172350252296 29.371078396061463 21.89723006644182 54-55 21.122543964727335 27.38761707495953 29.439623536723158 22.050215423589968 56-57 21.163627595800925 27.71781506227333 29.222494293735284 21.896063048190463 58-59 21.211558907631336 27.359544470594326 29.442056312211985 21.986840309562353 60-61 21.25838323450187 27.270498673677103 29.679373979907208 21.79174411191382 62-63 21.095263677985336 27.358829469105594 29.737373835445606 21.80853301746346 64-65 21.06219020261514 27.34636921982369 29.661662621663616 21.929777955897556 66-67 20.96102821518169 27.539077775817976 29.43032939197845 22.069564617021886 68-69 20.981395875699206 27.474121981347327 29.50104914531067 22.043432997642796 70-71 21.075943034026334 27.50990037724328 29.326154014618815 22.088002574111567 72-73 21.098550250902555 27.539515196824865 29.19162072315386 22.170313829118722 74-75 20.966807872458936 27.78212499207656 29.08720637759265 22.163860757871852 76-77 21.01255027675986 27.566592686031093 29.193600283947553 22.2272567532615 78-79 21.11745673146428 27.5783645453702 29.12372718975149 22.180451533414033 80-81 20.996539374293196 27.842137642335125 29.047868078175455 22.113454905196225 82-83 21.080727892075526 27.861306686061017 28.94236896343524 22.11559645842822 84-85 21.087564199849957 27.644241011212017 28.91480882831195 22.353385960626067 86-87 21.034693941942777 27.56799919313278 29.092255503996718 22.305051360927724 88-89 21.083959958889874 27.57862607936325 29.037140154942342 22.30027380680453 90-91 21.01310203839453 27.673781359397164 29.062494943120214 22.25062165908809 92-93 21.120077498933366 27.441812422159906 29.13211227204887 22.305997806857857 94-95 20.94293363423383 27.394503909270302 29.042300917938903 22.620261538556967 96-97 21.046174376600337 27.39282499068184 29.01774468730093 22.543255945416895 98-99 21.049863428693648 27.41303171038198 28.892322688584247 22.644782172340122 100-101 21.145582356935698 27.028455633171145 29.27758171616211 22.548380293731043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2661.0 1 1645.0 2 706.0 3 764.5 4 601.5 5 565.0 6 1109.0 7 2403.5 8 3937.5 9 4407.0 10 3593.0 11 2852.5 12 2851.0 13 3044.5 14 3247.5 15 3463.0 16 3565.0 17 3765.5 18 3965.5 19 4122.0 20 4459.0 21 5056.0 22 5922.5 23 6911.5 24 8029.5 25 9733.5 26 12097.0 27 14835.0 28 17261.0 29 19986.0 30 23477.0 31 27144.5 32 31664.0 33 36346.5 34 41371.5 35 45685.0 36 50276.5 37 54639.0 38 57046.5 39 58781.5 40 60607.0 41 63861.0 42 66310.0 43 68351.5 44 70833.0 45 72432.5 46 71514.5 47 70571.0 48 70932.0 49 69274.5 50 65697.0 51 61415.0 52 57687.0 53 55097.5 54 52274.5 55 47468.0 56 41882.0 57 36981.5 58 33183.0 59 29454.0 60 25090.5 61 20621.5 62 16161.5 63 11956.0 64 8805.5 65 6883.5 66 5089.5 67 3808.5 68 2821.0 69 1840.0 70 1380.5 71 1016.5 72 743.5 73 592.0 74 463.0 75 377.5 76 265.0 77 159.5 78 106.5 79 76.0 80 48.5 81 34.5 82 31.0 83 22.5 84 18.0 85 15.5 86 10.0 87 9.0 88 8.0 89 6.0 90 7.0 91 5.5 92 3.5 93 4.5 94 3.0 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2859487683201075 2 0.24803766984602926 3 0.24335263352892014 4 0.2503532625085085 5 0.25180723929657683 6 0.19450978364825394 7 0.1854628169669398 8 0.1828241183515565 9 0.19531754853051414 10-11 0.19792932164982208 12-13 0.19275962640335687 14-15 0.19547910150696618 16-17 0.18150476904386487 18-19 0.18613595436882327 20-21 0.17585041486804354 22-23 0.18438579712392622 24-25 0.1851935620061864 26-27 0.1830933733123099 28-29 0.1915749045760419 30-31 0.1879668881019464 32-33 0.1927865518994322 34-35 0.18920546092141202 36-37 0.17875836844418022 38-39 0.1696036997785647 40-41 0.16744966009253753 42-43 0.1784891134834268 44-45 0.17811215653837206 46-47 0.1782737095148241 48-49 0.19030940626050094 50-51 0.1864859858178027 52-53 0.19927559645358905 54-55 0.17784290157761865 56-57 0.16653419322597598 58-59 0.1687151584080785 60-61 0.1603682546247232 62-63 0.16413782407527078 64-65 0.16384164361844203 66-67 0.16397627109881874 68-69 0.16618416177699658 70-71 0.1686882329120032 72-73 0.16526869491043503 74-75 0.17762749760901594 76-77 0.16761121306898957 78-79 0.17253857885077675 80-81 0.16804202100619503 82-83 0.15226368030604598 84-85 0.15105203298265568 86-87 0.15571014380368944 88-89 0.15786418348971662 90-91 0.165107141933983 92-93 0.16236074133429834 94-95 0.1607721370658533 96-97 0.16526869491043503 98-99 0.17138078251953714 100-101 0.15977589371106574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1856976.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91508769157618 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2103142406264 40.549706875943734 2 13.687529004318618 14.759286530976615 3 4.624689708602826 7.480216535569538 4 2.1228453981102295 4.578135831790879 5 1.1602058954185035 3.127630129588614 6 0.6983399018743255 2.2590634228848576 7 0.4458398073842077 1.6826244618060493 8 0.3402950374605303 1.4677629428554153 9 0.2394177155361573 1.1617404415243886 >10 1.3250657775453696 13.094208755926504 >50 0.10319111069996688 3.8136939790226414 >100 0.0400738303425657 3.6759687645574886 >500 0.0014949355092870882 0.5395335823378529 >1k 3.9864946914322334E-4 0.38827503893768794 >5k 1.9932473457161167E-4 0.7613973570090006 >10k+ 9.966236728580584E-5 0.6607553492688554 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12297 0.6622056504769044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7939 0.4275230266842437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6231 0.3355455320908832 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2317 0.12477274881312413 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2058 0.11082534184609819 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.3850992150679384E-5 0.0 9 0.0 0.0 0.0 1.0770198430135877E-4 0.0 10-11 0.0 0.0 0.0 1.0770198430135877E-4 0.0 12-13 0.0 0.0 0.0 1.0770198430135877E-4 0.0 14-15 0.0 0.0 0.0 1.8847847252737786E-4 0.0 16-17 0.0 0.0 0.0 2.4232946467805724E-4 0.0 18-19 0.0 0.0 0.0 2.6925496075339694E-4 0.0 20-21 0.0 0.0 0.0 2.6925496075339694E-4 0.0 22-23 0.0 0.0 0.0 4.038824411300954E-4 0.0 24-25 0.0 0.0 0.0 7.269883940341716E-4 0.0 26-27 0.0 0.0 0.0 9.154668665615495E-4 0.0 28-29 0.0 0.0 0.0 0.0020463377017258164 0.0 30-31 0.0 0.0 0.0 0.003958047923074935 0.0 32-33 0.0 0.0 0.0 0.006892926995286961 0.0 34-35 0.0 0.0 0.0 0.01130870835164267 0.0 36-37 0.0 0.0 0.0 0.017851603897950215 0.0 38-39 0.0 0.0 0.0 0.02867565332023677 0.0 40-41 0.0 0.0 0.0 0.04367315463420098 0.0 42-43 0.0 0.0 0.0 0.05823984801095976 0.0 44-45 0.0 0.0 0.0 0.07687229129509482 0.0 46-47 0.0 0.0 0.0 0.1029092459999483 0.0 48-49 0.0 0.0 0.0 0.12926930665770586 0.0 50-51 0.0 0.0 0.0 0.15950663875031235 0.0 52-53 0.0 0.0 0.0 0.18742837818043961 2.6925496075339692E-5 54-55 0.0 0.0 0.0 0.22197378964510042 5.3850992150679384E-5 56-57 0.0 0.0 0.0 0.26976654517882837 5.3850992150679384E-5 58-59 0.0 0.0 0.0 0.32132887016310385 5.3850992150679384E-5 60-61 0.0 0.0 0.0 0.3750721603294819 5.3850992150679384E-5 62-63 0.0 0.0 0.0 0.4330158278836129 5.3850992150679384E-5 64-65 0.0 0.0 0.0 0.49575223373915445 5.3850992150679384E-5 66-67 0.0 0.0 0.0 0.5701204538992426 5.3850992150679384E-5 68-69 0.0 0.0 0.0 0.6566320727893091 5.3850992150679384E-5 70-71 0.0 0.0 0.0 0.7582219694815657 5.3850992150679384E-5 72-73 0.0 0.0 0.0 0.8629621492146371 1.0770198430135877E-4 74-75 0.0 0.0 0.0 0.9771801035662281 1.0770198430135877E-4 76-77 0.0 0.0 0.0 1.1052377629005437 1.0770198430135877E-4 78-79 0.0 0.0 0.0 1.2520086420072203 1.0770198430135877E-4 80-81 0.0 0.0 0.0 1.4176542938627101 1.0770198430135877E-4 82-83 0.0 0.0 0.0 1.596843470244096 1.0770198430135877E-4 84-85 0.0 0.0 0.0 1.7872875039849734 1.0770198430135877E-4 86-87 0.0 0.0 0.0 1.9822011700743576 1.0770198430135877E-4 88-89 0.0 0.0 0.0 2.2016170375923005 1.0770198430135877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4100 0.0 56.242996 1 GTATCAA 8185 0.0 48.62027 1 ATCAACG 10730 0.0 36.849773 3 TATCAAC 10850 0.0 36.530807 2 TCAACGC 10935 0.0 36.164806 4 CAACGCA 11200 0.0 35.349648 5 AACGCAG 11620 0.0 34.095367 6 GTGTACG 175 1.5097612E-10 29.885822 1 ACGCAGA 13390 0.0 29.481133 7 CGCAGAG 13515 0.0 29.13895 8 GTGGTAT 1815 0.0 28.81553 1 GTACATG 9055 0.0 27.408966 1 GCAGAGT 14465 0.0 26.99534 9 TACATGG 9090 0.0 26.507393 2 TGGTATC 1845 0.0 26.274035 2 ACATGGG 8965 0.0 25.28595 3 GAGTACT 8830 0.0 23.831701 12-13 AATACCG 105 0.0021889636 22.632828 5 AGTACTT 9340 0.0 22.504969 12-13 AGAGTAC 13585 0.0 22.256187 10-11 >>END_MODULE