FastQCFastQC Report
Thu 26 May 2016
SRR937826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118230.6366802801974824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89860.48390501546600495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72140.3884810573750011No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.13484288434530117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.10538639163887956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.055.3171771
GTATCAA96050.043.6799281
TATCAAC129200.032.3578952
ATCAACG130000.032.2318573
TCAACGC133600.031.3633334
CAACGCA137900.030.385365
AACGCAG143050.029.2859256
TGGTATC21350.027.5920472
CGTACAC1606.236951E-826.7228413
ACGCAGA169550.024.5418857
CGCAGAG170100.024.294988
GCAGAGT177650.022.7811689
GTGGTAT27150.022.750561
GAGTACT113250.022.18804612-13
AGTACTT117000.021.76108412-13
GTACTTT119150.021.04835114-15
GAGTACA78900.020.95661
GGACCGA3650.020.821186
AGAGTAC165050.020.47674610-11
AGTACAT75900.020.0920092
Produced by FastQC (version 0.11.5)