##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937826_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856976 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.093132598374993 31.0 31.0 33.0 30.0 34.0 2 31.35904556655552 31.0 31.0 34.0 30.0 34.0 3 31.401170505165386 31.0 31.0 34.0 28.0 34.0 4 35.251319887817615 37.0 35.0 37.0 33.0 37.0 5 35.09205504002206 35.0 35.0 37.0 32.0 37.0 6 35.19337137367419 37.0 35.0 37.0 32.0 37.0 7 35.11585933259234 37.0 35.0 37.0 32.0 37.0 8 35.08702428033534 37.0 35.0 37.0 32.0 37.0 9 36.543899867311154 38.0 35.0 39.0 32.0 39.0 10-11 36.40139237125305 38.0 35.0 39.0 32.0 39.0 12-13 36.23293004325312 38.0 35.0 39.0 32.0 39.0 14-15 37.052839132008174 38.5 36.0 40.0 31.5 41.0 16-17 36.97475384711488 38.0 36.0 40.0 31.0 41.0 18-19 36.94059858608835 38.5 36.0 40.0 31.0 41.0 20-21 36.78674657076882 38.5 36.0 40.0 31.0 41.0 22-23 36.54195019214034 38.0 35.0 40.0 30.0 41.0 24-25 36.60130717898346 38.0 35.0 40.0 30.0 41.0 26-27 36.339904231395565 38.0 35.0 40.0 30.0 41.0 28-29 36.08352342733562 38.0 34.5 40.0 29.5 41.0 30-31 36.046677501486286 38.0 35.0 40.0 30.0 41.0 32-33 35.88752277896968 38.0 34.5 40.0 30.0 41.0 34-35 35.638896787034405 38.0 34.0 40.0 29.0 41.0 36-37 35.33221888705077 38.0 34.0 40.0 27.0 41.0 38-39 35.07709227259803 38.0 34.0 40.0 27.0 41.0 40-41 34.75154148465032 38.0 33.0 40.0 26.0 41.0 42-43 34.38225130534805 37.0 33.0 40.0 24.5 41.0 44-45 33.83817211423303 37.0 33.0 40.0 24.0 40.5 46-47 33.28692024021851 37.0 32.0 39.0 22.0 40.0 48-49 32.76258309208089 37.0 31.0 39.0 18.5 40.0 50-51 32.34701013906481 36.0 31.0 39.0 16.0 40.0 52-53 31.906125065698212 36.0 30.0 39.0 13.5 40.0 54-55 31.50765114896477 35.5 30.0 39.0 10.0 40.0 56-57 31.073180805783167 35.0 29.0 38.0 8.0 40.0 58-59 30.635647687422995 35.0 28.0 38.0 7.5 40.0 60-61 30.055891944753192 34.0 28.0 38.0 6.0 40.0 62-63 29.492305500986554 34.0 26.5 38.0 2.0 40.0 64-65 28.892945573879253 33.5 26.0 37.0 2.0 39.0 66-67 28.22463214387262 33.0 25.5 37.0 2.0 39.0 68-69 27.570004405011158 32.0 24.5 36.0 2.0 39.0 70-71 26.98440017533883 31.5 22.5 36.0 2.0 38.5 72-73 26.258293591301126 31.0 21.5 35.0 2.0 38.0 74-75 25.524476083697365 31.0 20.0 34.5 2.0 37.0 76-77 24.8226145626007 29.5 20.0 34.0 2.0 36.0 78-79 25.3260949522234 31.0 20.0 34.0 2.0 36.0 80-81 24.958373721577445 31.0 19.0 34.0 2.0 36.0 82-83 24.37601562971196 30.5 17.0 34.0 2.0 36.0 84-85 23.668632227880167 30.0 9.0 34.0 2.0 35.0 86-87 22.866160359638464 29.0 4.5 34.0 2.0 35.0 88-89 22.057108169410913 29.0 2.0 34.0 2.0 35.0 90-91 21.023449953041933 28.0 2.0 33.0 2.0 35.0 92-93 20.018768686294276 26.0 2.0 33.0 2.0 34.0 94-95 19.010964062001875 25.0 2.0 32.0 2.0 34.0 96-97 17.888670612867372 24.0 2.0 32.0 2.0 34.0 98-99 16.447751613375722 10.5 2.0 31.5 2.0 34.0 100-101 14.148320710660773 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46612987353621804 1101 2 0.43603666263478047 1101 3 0.39394857515176085 1101 4 0.34535514316183935 1101 5 0.2172125829611744 1101 6 0.23635890754228228 1101 7 0.38426146852378196 1101 8 0.2725186703206859 1101 9 0.4373506005931489 1101 10-11 0.5159519304239666 1101 12-13 0.4833206386016755 1101 14-15 0.5885983128710066 1101 16-17 0.5786437495443053 1101 18-19 0.5394044092347059 1101 20-21 0.6450851063872349 1101 22-23 0.5361434215795882 1101 24-25 0.5394756962371474 1101 26-27 0.30997399875478493 1101 28-29 0.36387165435297675 1101 30-31 0.5339780537554333 1101 32-33 0.7006534494671754 1101 34-35 0.6616202405392002 1101 36-37 0.6515927980591059 1101 38-39 0.615566060092867 1101 40-41 0.5039634047899639 1101 42-43 0.6718191119493042 1101 44-45 0.6369120577215241 1101 46-47 0.6569618237863324 1101 48-49 0.6828277636946396 1101 50-51 0.7102944609139499 1101 52-53 0.6098665920932831 1101 54-55 0.8625091247026937 1101 56-57 0.8563844291171705 1101 58-59 0.5975548427480568 1101 60-61 0.6056237851734423 1101 62-63 0.6666407097037599 1101 64-65 0.8807951802453822 1101 66-67 0.7362870229451097 1101 68-69 0.7888591213794598 1101 70-71 0.852022315588858 1101 72-73 0.9456653051606949 1101 74-75 0.9515504472797325 1101 76-77 0.8149370618407445 1101 78-79 0.9160771746459879 1101 80-81 0.7754714486823246 1101 82-83 0.9013276248750124 1101 84-85 0.9508390477731616 1101 86-87 0.6707444637473579 1101 88-89 1.1843957490052262 1101 90-91 0.9271966023766076 1101 92-93 1.1642157580187558 1101 94-95 1.133027367956064 1101 96-97 1.1594787614199582 1101 98-99 1.2337023166403505 1101 100-101 1.092245309343328 1102 1 0.3017018278146679 1102 2 0.36715238132547867 1102 3 0.29215298008463364 1102 4 0.22107937242284947 1102 5 0.1986466802730149 1102 6 0.2376347297615098 1102 7 0.34446192190488034 1102 8 0.30071421710290736 1102 9 0.3397950241299128 1102 10-11 0.3147470266653798 1102 12-13 0.28776737697379673 1102 14-15 0.36580579419167236 1102 16-17 0.3705303758670411 1102 18-19 0.30565343510440357 1102 20-21 0.2926806244115241 1102 22-23 0.4296563795095878 1102 24-25 0.34028943897443753 1102 26-27 0.396683133656083 1102 28-29 0.3106633706507296 1102 30-31 0.3651654897379828 1102 32-33 0.36848274101038925 1102 34-35 0.3495476818117922 1102 36-37 0.4716631662137871 1102 38-39 0.45511282100978434 1102 40-41 0.33937621413735286 1102 42-43 0.5255249787433129 1102 44-45 0.5850744796357645 1102 46-47 0.583074906334005 1102 48-49 0.30768437349044575 1102 50-51 0.45151415192604105 1102 52-53 0.39438923718322627 1102 54-55 0.4789503138605973 1102 56-57 0.40834103305487446 1102 58-59 0.41765343627255547 1102 60-61 0.4554391664023285 1102 62-63 0.5992033780130619 1102 64-65 0.7286138384574556 1102 66-67 0.7190971514149211 1102 68-69 0.8790823148226501 1102 70-71 0.7835718883916734 1102 72-73 0.7338660354699478 1102 74-75 0.584112849311957 1102 76-77 0.5551018518457695 1102 78-79 1.0467778561377763 1102 80-81 1.1256240254455676 1102 82-83 1.1638680324209503 1102 84-85 1.1377707903080854 1102 86-87 1.2115833785440238 1102 88-89 1.0513687822383808 1102 90-91 1.2318510368662352 1102 92-93 1.1659511744672244 1102 94-95 1.2422653184746828 1102 96-97 1.2871983823124111 1102 98-99 1.2546904531091236 1102 100-101 1.3079438517426265 1103 1 0.36622772213149446 1103 2 0.375087278385525 1103 3 0.3176115685742005 1103 4 0.22109357342592517 1103 5 0.2722750711954589 1103 6 0.19076518502488682 1103 7 0.22240949595791193 1103 8 0.23031083934237273 1103 9 0.24988431726632854 1103 10-11 0.21949518399863877 1103 12-13 0.2862134062347934 1103 14-15 0.343139088270199 1103 16-17 0.4923808704775823 1103 18-19 0.37614652164046447 1103 20-21 0.3209949999830357 1103 22-23 0.526664470617682 1103 24-25 0.41982821810380955 1103 26-27 0.2886810416058694 1103 28-29 0.3135643584863601 1103 30-31 0.42974541713969217 1103 32-33 0.41783070714966186 1103 34-35 0.38978535811205006 1103 36-37 0.37046976240652896 1103 38-39 0.2982558147197736 1103 40-41 0.4346848608163967 1103 42-43 0.4742356315738121 1103 44-45 0.5976144849005323 1103 46-47 0.5577834394050285 1103 48-49 0.5028913617694215 1103 50-51 0.4544231061780195 1103 52-53 0.3191348314086113 1103 54-55 0.44841209584403074 1103 56-57 0.5738350201423543 1103 58-59 0.6380500598974805 1103 60-61 0.6169870064643952 1103 62-63 0.7028282706396247 1103 64-65 0.8882443699213347 1103 66-67 0.8892703690158505 1103 68-69 0.7624740685426232 1103 70-71 0.958243533491288 1103 72-73 0.9885007579615674 1103 74-75 0.8872521369065147 1103 76-77 0.9442910784799246 1103 78-79 0.9771486062278321 1103 80-81 0.8811935895786824 1103 82-83 1.1595784635772581 1103 84-85 1.1981517235564425 1103 86-87 1.014779123784951 1103 88-89 1.0000861892044917 1103 90-91 1.2545574016865366 1103 92-93 1.2608782990758343 1103 94-95 1.321852415651108 1103 96-97 1.287322727680806 1103 98-99 1.2502841589474656 1103 100-101 1.2289306829100166 1104 1 0.3705850885471733 1104 2 0.32732067673443055 1104 3 0.18652486350298503 1104 4 0.26379122832931046 1104 5 0.30382557922412445 1104 6 0.3177978666904977 1104 7 0.2402642088310003 1104 8 0.3443309563704062 1104 9 0.35352840236112826 1104 10-11 0.40082342948534944 1104 12-13 0.40737909388788296 1104 14-15 0.4146926013791514 1104 16-17 0.3685035231129845 1104 18-19 0.43418000844079074 1104 20-21 0.38565667330245645 1104 22-23 0.3066146316446279 1104 24-25 0.25121628169811316 1104 26-27 0.5243402881812074 1104 28-29 0.4040597998362685 1104 30-31 0.5852597394657337 1104 32-33 0.5236109507312605 1104 34-35 0.6172543434155031 1104 36-37 0.7443201774478112 1104 38-39 0.6645963640257762 1104 40-41 0.48536298836788205 1104 42-43 0.5032715110984469 1104 44-45 0.5322554061560822 1104 46-47 0.49201895832001696 1104 48-49 0.41715018423155215 1104 50-51 0.5732456817019269 1104 52-53 0.392709686257561 1104 54-55 0.5482360434989317 1104 56-57 0.765641356892786 1104 58-59 0.6933577120792833 1104 60-61 0.7507470989981897 1104 62-63 0.598292583893727 1104 64-65 0.7122473069620696 1104 66-67 0.5833872156093385 1104 68-69 0.5598182579647393 1104 70-71 0.6687905148030886 1104 72-73 0.8980081515930642 1104 74-75 0.8304121772766493 1104 76-77 0.6505940926321507 1104 78-79 1.1409906864237875 1104 80-81 1.2757223589699542 1104 82-83 1.3264015267179659 1104 84-85 1.2326973894368756 1104 86-87 1.364423050120795 1104 88-89 1.597165874861755 1104 90-91 1.5044510898839434 1104 92-93 1.5408100327236554 1104 94-95 1.4395906107885459 1104 96-97 1.6186455867028506 1104 98-99 1.5385890101676445 1104 100-101 1.396645347701817 1105 1 0.2276996693357205 1105 2 0.40625743154742366 1105 3 0.2868124736610653 1105 4 0.16165349870296808 1105 5 0.12139497360047358 1105 6 0.11315837930169437 1105 7 0.21791790877200867 1105 8 0.22800041211947786 1105 9 0.3838511081831939 1105 10-11 0.17205436967971366 1105 12-13 0.31044696660800497 1105 14-15 0.141216696876306 1105 16-17 0.19279319342825119 1105 18-19 0.21079065139455366 1105 20-21 0.49965045484535153 1105 22-23 0.42191370077533463 1105 24-25 0.4072403235686082 1105 26-27 0.19986525213218442 1105 28-29 0.22278570508392903 1105 30-31 0.33489673544968923 1105 32-33 0.46655303171346674 1105 34-35 0.4761245453198484 1105 36-37 0.41704873498026274 1105 38-39 0.3469713878598455 1105 40-41 0.3541957372700253 1105 42-43 0.47450397816274403 1105 44-45 0.2865584794100897 1105 46-47 0.5374527420675719 1105 48-49 0.3624167384808672 1105 50-51 0.5661952666912313 1105 52-53 0.6807534195364795 1105 54-55 0.8306167651646952 1105 56-57 0.7047015572777049 1105 58-59 0.5603572860453312 1105 60-61 0.8041566367960407 1105 62-63 0.762751837321364 1105 64-65 1.0024999741431522 1105 66-67 0.8822181918527328 1105 68-69 0.7101129066207967 1105 70-71 0.9277548240322382 1105 72-73 1.0299986090284001 1105 74-75 0.7503391146883907 1105 76-77 0.8109808860265382 1105 78-79 1.0986634312741366 1105 80-81 0.97638686393638 1105 82-83 1.1039605048621226 1105 84-85 1.035843355967888 1105 86-87 0.909681150724392 1105 88-89 1.1472346398697688 1105 90-91 1.250878865956924 1105 92-93 1.0895250040929838 1105 94-95 1.0856805017178992 1105 96-97 1.4065757357551476 1105 98-99 1.7847076881813102 1105 100-101 1.7836443109198914 1106 1 0.059170525415471076 1106 2 0.09861736494950435 1106 3 0.1573751918596642 1106 4 0.21786137608981448 1106 5 0.16762008694563946 1106 6 0.06628915754268405 1106 7 0.1357897077792316 1106 8 0.031915931487127125 1106 9 0.10400137706740509 1106 10-11 -0.07250400368894105 1106 12-13 -0.22588834364739085 1106 14-15 0.2408551571741384 1106 16-17 0.20922488168873343 1106 18-19 0.215440429917372 1106 20-21 0.2586934274453867 1106 22-23 0.2371619034623862 1106 24-25 0.39884451395915477 1106 26-27 0.2233720528418246 1106 28-29 0.3466358605822535 1106 30-31 0.32334799173600715 1106 32-33 0.44749328745077577 1106 34-35 0.12224623069681684 1106 36-37 0.07838221248962185 1106 38-39 0.17744604464760272 1106 40-41 0.22265039024566846 1106 42-43 0.14518476593401175 1106 44-45 0.44083741195363757 1106 46-47 0.3940707065628004 1106 48-49 0.3741367421300694 1106 50-51 0.6413347997564145 1106 52-53 0.6260393319914925 1106 54-55 0.8225509516616718 1106 56-57 0.7120412978911936 1106 58-59 0.6717323465259533 1106 60-61 0.8024376228297001 1106 62-63 0.8008227580766807 1106 64-65 0.7248773349236934 1106 66-67 0.750388972830617 1106 68-69 0.4436941494018143 1106 70-71 0.8286471473514432 1106 72-73 0.7015777478169625 1106 74-75 0.3864452502334004 1106 76-77 0.45153988171429305 1106 78-79 0.3978287783636567 1106 80-81 0.5684207518179356 1106 82-83 0.6410603756738951 1106 84-85 0.5776907517579453 1106 86-87 0.7989264747783302 1106 88-89 0.5887910159525127 1106 90-91 0.8291738786069409 1106 92-93 1.1535383432259394 1106 94-95 0.9773428105217583 1106 96-97 1.0563516112782985 1106 98-99 1.301164525790238 1106 100-101 1.2595885009520345 1107 1 0.2538893591527476 1107 2 0.42053497367546555 1107 3 0.40185151575617084 1107 4 0.2536820706448779 1107 5 0.21235286296948885 1107 6 0.21565998798728714 1107 7 0.24884637266968923 1107 8 0.22527324364346413 1107 9 0.40822970970361894 1107 10-11 0.4091475652002785 1107 12-13 0.40553764190993036 1107 14-15 0.5030243382271422 1107 16-17 0.5904701427437331 1107 18-19 0.4547424704569565 1107 20-21 0.5943255668988172 1107 22-23 0.5374429455814607 1107 24-25 0.44292386466877787 1107 26-27 0.5464258146808305 1107 28-29 0.5227261044194691 1107 30-31 0.6364993877790752 1107 32-33 0.721130715423584 1107 34-35 0.6342061580823284 1107 36-37 0.6997222428952696 1107 38-39 0.8444283226644345 1107 40-41 0.9314802981692836 1107 42-43 0.8374059249772969 1107 44-45 0.8914769427288434 1107 46-47 0.6047317332880624 1107 48-49 0.9766127871808408 1107 50-51 1.0286622639600722 1107 52-53 0.840560390573927 1107 54-55 1.0394426764371154 1107 56-57 0.8565805530675092 1107 58-59 0.5575611342918805 1107 60-61 0.8087318251303692 1107 62-63 0.8354845000603497 1107 64-65 1.1633351509122498 1107 66-67 1.2092628085064483 1107 68-69 1.1065025747446917 1107 70-71 0.9913177016241832 1107 72-73 1.2745296879911834 1107 74-75 0.9248346439731243 1107 76-77 1.2117110662547752 1107 78-79 1.379618457060797 1107 80-81 1.4002160169792184 1107 82-83 1.443826916859983 1107 84-85 1.5817914313011734 1107 86-87 1.7300203044570672 1107 88-89 1.9002108468497632 1107 90-91 1.8440812626711 1107 92-93 2.013388759680133 1107 94-95 2.084611039106502 1107 96-97 1.8351550861544865 1107 98-99 1.9749365063779898 1107 100-101 1.8448030880216155 1108 1 0.07104110919702933 1108 2 0.2180762538832326 1108 3 0.09396444439097351 1108 4 0.10409372803059824 1108 5 -0.1411688242985818 1108 6 0.02092473364720604 1108 7 0.0014751740607934494 1108 8 -0.014186890857189383 1108 9 0.14995214794193146 1108 10-11 0.03358996940395542 1108 12-13 0.09863068848855505 1108 14-15 0.025698003692944837 1108 16-17 -0.14892418832315713 1108 18-19 -0.04502505896293485 1108 20-21 -0.07003440378660031 1108 22-23 0.01137772142723037 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-0.6461848229923746 2316 52-53 -0.5063686419519691 2316 54-55 -0.3042359545570399 2316 56-57 -0.356145436512584 2316 58-59 -0.07383244871762074 2316 60-61 -0.008857200472853322 2316 62-63 8.148780752001983E-4 2316 64-65 -0.25773490101900265 2316 66-67 -0.45227075943474304 2316 68-69 -0.05383214817176096 2316 70-71 0.028815408559591305 2316 72-73 0.2705300784432829 2316 74-75 0.37566432663542315 2316 76-77 0.3726564315715244 2316 78-79 0.5027370922287524 2316 80-81 0.034846537654946275 2316 82-83 -0.20109392402574144 2316 84-85 0.36721668468831936 2316 86-87 0.27678676080693165 2316 88-89 0.23853731080671636 2316 90-91 0.8730013652881965 2316 92-93 0.8471577051621324 2316 94-95 1.2242711550416026 2316 96-97 1.450229622674943 2316 98-99 1.0358804131093073 2316 100-101 1.2981572454669354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 23.0 4 72.0 5 145.0 6 272.0 7 714.0 8 1690.0 9 2755.0 10 4246.0 11 6599.0 12 9410.0 13 13534.0 14 17489.0 15 23262.0 16 30304.0 17 37359.0 18 33321.0 19 27548.0 20 27377.0 21 29037.0 22 31925.0 23 35121.0 24 40072.0 25 46550.0 26 54807.0 27 64082.0 28 74532.0 29 86660.0 30 99075.0 31 112901.0 32 129951.0 33 146935.0 34 164288.0 35 178928.0 36 171480.0 37 120290.0 38 33340.0 39 875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.374979735410435 23.489904329593518 16.685795721088155 19.449320213907892 2 18.083917078088245 24.10262706680108 36.85858083249326 20.95487502261742 3 21.638836473923195 26.527267988385418 30.280951396248522 21.55294414144286 4 15.740931213899604 19.6291385878316 35.53738501454517 29.09254518372363 5 13.524281346158714 38.9544826085528 33.50821999632668 14.013016048961802 6 31.081500245560523 38.461886421795434 16.296979605552252 14.159633727091789 7 27.466913950422622 34.54029562040651 20.341296818052577 17.65149361111829 8 26.402656792548747 36.4418818552313 18.97822050473458 18.177240847485372 9 26.40362611040746 17.15746460912796 20.40683347549995 36.03207580496463 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27.19432022815588 31.159530333186858 20.14363675136351 36-37 21.679117016051904 26.97269646995976 31.012678677591953 20.335507836396378 38-39 21.394908761536573 27.256809396144106 31.04232179817224 20.305960044147078 40-41 21.584411730890345 27.13891867920723 30.90744256541806 20.369227024484367 42-43 21.621984805842406 27.213139652841157 30.620959061518 20.543916479798437 44-45 21.666677436865097 27.46982190399876 30.31229267368022 20.551207985455925 46-47 21.876938635717423 27.830623551408312 29.5696336409302 20.722804171944066 48-49 21.941425198817864 28.042446429033006 29.247550856876988 20.768577515272142 50-51 22.10386671664039 28.177316238874383 28.88133718475629 20.837479859728937 52-53 22.27255494955239 28.356774670216524 28.60750488967009 20.763165490560997 54-55 22.077237401021875 28.401201738740834 28.681011493955765 20.840549366281525 56-57 22.052331317152188 28.54382609145191 28.61353620079096 20.79030639060494 58-59 22.14533198059641 28.237467797106692 28.73677419632779 20.880426025969104 60-61 22.08240709626834 28.264904877607467 28.926679720147163 20.72600830597703 62-63 21.952464652208754 28.32357553355563 29.04447876547678 20.67948104875884 64-65 21.980359466142804 28.178716364670304 28.871778633649548 20.969145535537347 66-67 21.94056358294345 28.50314166688207 28.636584425431455 20.919710324743022 68-69 21.974022798912962 28.362748115281068 28.598454098319603 21.064774987486366 70-71 22.115875487889987 28.46431510154143 28.373872360224368 21.045937050344214 72-73 22.154244319797346 28.521100972764323 28.17672387796073 21.147930829477602 74-75 22.114212061494616 28.80748830558077 27.828934738234295 21.249364894690316 76-77 21.979605552252696 28.564989531367125 28.30179280723068 21.1536121091495 78-79 22.041749597194578 28.558931294750174 28.175593007125567 21.22372610092968 80-81 21.96151753213761 28.78252836399834 28.08876584532214 21.167188258541913 82-83 22.133512625388207 28.705209073240663 27.99874847396217 21.162529827408957 84-85 22.003993069345587 28.658686810620004 27.983930790388094 21.35338932964632 86-87 22.11609089185859 28.487686432134822 28.049339356028298 21.34688331997829 88-89 22.114852319039127 28.488413420528858 27.9960537992952 21.40068046113682 90-91 22.150667007402937 28.557352619221565 27.947447265093267 21.344533108282235 92-93 22.141500446705766 28.431596934074417 27.910251722334806 21.516650896885015 94-95 22.054781537295042 28.310893624904143 27.921712504631184 21.712612333169627 96-97 22.172230551175677 28.364340734613698 27.753428154160314 21.710000560050318 98-99 22.108059204769123 28.344539014348836 27.60841058835884 21.9389911925232 100-101 22.44526643353536 27.97947331355712 27.904794093528114 21.670466159379405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1098.0 1 742.0 2 439.0 3 521.5 4 512.5 5 556.0 6 950.5 7 1923.5 8 2993.5 9 3349.5 10 3059.0 11 2825.0 12 3003.0 13 3290.0 14 3651.5 15 4005.0 16 4303.0 17 4597.0 18 4772.5 19 5070.0 20 5454.0 21 6035.5 22 6966.5 23 8006.5 24 9164.0 25 10885.5 26 13393.5 27 16032.5 28 18394.0 29 21340.5 30 25166.5 31 28968.5 32 33153.5 33 37752.0 34 42399.5 35 46828.5 36 51818.0 37 55962.0 38 58220.0 39 60072.0 40 61704.5 41 64330.5 42 66805.5 43 68429.5 44 69952.0 45 71342.5 46 70851.0 47 69852.5 48 69513.5 49 67748.0 50 64688.5 51 60324.0 52 55940.0 53 52607.0 54 49810.5 55 45301.0 56 39774.5 57 35182.5 58 31487.5 59 28096.5 60 23757.0 61 19542.5 62 15776.0 63 12020.0 64 8908.5 65 6764.5 66 5120.0 67 3818.0 68 2824.5 69 1977.5 70 1416.0 71 1038.5 72 808.0 73 633.5 74 480.5 75 383.0 76 296.5 77 191.0 78 124.0 79 86.5 80 53.0 81 38.5 82 21.5 83 12.5 84 8.5 85 3.0 86 2.5 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015562936731546344 2 0.0 3 0.0 4 1.0770198430135875E-4 5 0.018578592291984386 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0022348161742531945 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.423923626368892E-4 24-25 2.692549607533969E-5 26-27 4.5773343328077475E-4 28-29 0.001157796331239607 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.077648822601908E-5 40-41 8.346903783355306E-4 42-43 0.01483594833751217 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.077648822601908E-5 70-71 0.0 72-73 0.0 74-75 2.692549607533969E-5 76-77 0.0 78-79 0.0 80-81 2.692549607533969E-5 82-83 0.002315592662479214 84-85 4.5773343328077475E-4 86-87 0.0 88-89 0.0 90-91 0.011443335832019369 92-93 0.0026386986153832896 94-95 0.0 96-97 0.0 98-99 0.0016693807566710611 100-101 0.019844090607525355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1856976.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.1462211169215 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12467965462129 43.30259317460104 2 12.814441738165245 14.389649586418674 3 4.211268948168189 7.093405126400297 4 1.912503685413679 4.295194192326548 5 1.04229218868433 2.9260383847155227 6 0.6365641829524986 2.1444404022698076 7 0.409568005653461 1.6096987065485044 8 0.2992325525891227 1.344062165043978 9 0.22081254068432613 1.115801076118626 >10 1.2038247200082028 12.425676062284126 >50 0.0860039680313224 3.33244946205164 >100 0.036694984856156763 3.605036205455675 >500 0.0014405660265413259 0.5270293187066982 >1k 3.841509404110202E-4 0.3778822862181851 >5k 1.920754702055101E-4 0.8735292575249142 >10k+ 9.603773510275505E-5 0.6375145933158679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11823 0.6366802801974824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8986 0.48390501546600495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7214 0.3884810573750011 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2504 0.13484288434530117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1957 0.10538639163887956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.3850992150679384E-5 0.0 9 0.0 0.0 0.0 1.0770198430135877E-4 0.0 10-11 0.0 0.0 0.0 1.0770198430135877E-4 0.0 12-13 0.0 0.0 0.0 1.0770198430135877E-4 0.0 14-15 0.0 0.0 0.0 1.8847847252737786E-4 0.0 16-17 0.0 0.0 0.0 2.4232946467805724E-4 0.0 18-19 0.0 0.0 0.0 2.6925496075339694E-4 0.0 20-21 0.0 0.0 0.0 2.6925496075339694E-4 0.0 22-23 0.0 0.0 0.0 4.038824411300954E-4 0.0 24-25 0.0 0.0 0.0 7.808393861848511E-4 0.0 26-27 0.0 0.0 0.0 9.693178587122288E-4 0.0 28-29 0.0 0.0 0.0 0.0019924867095751373 0.0 30-31 0.0 0.0 0.0 0.0039849734191502745 0.0 32-33 0.0 0.0 0.0 0.006866001499211622 0.0 34-35 0.0 0.0 0.0 0.011497186824170048 0.0 36-37 0.0 0.0 0.0 0.017851603897950215 0.0 38-39 0.0 0.0 0.0 0.02886413179276415 0.0 40-41 0.0 0.0 0.0 0.04345775066559826 0.0 42-43 0.0 0.0 0.0 0.05807829503450772 2.6925496075339692E-5 44-45 0.0 0.0 0.0 0.07673766381471812 5.3850992150679384E-5 46-47 0.0 0.0 0.0 0.10199377913338675 5.3850992150679384E-5 48-49 0.0 0.0 0.0 0.12797688284608955 5.3850992150679384E-5 50-51 0.0 0.0 0.0 0.15708334410353175 5.3850992150679384E-5 52-53 0.0 0.0 0.0 0.18392806369064543 5.3850992150679384E-5 54-55 0.0 0.0 0.0 0.21631943546927906 5.3850992150679384E-5 56-57 0.0 0.0 0.0 0.26152734337977446 5.3850992150679384E-5 58-59 0.0 0.0 0.0 0.30985860883500915 5.3850992150679384E-5 60-61 0.0 0.0 0.0 0.35894378818035344 5.3850992150679384E-5 62-63 0.0 0.0 0.0 0.412013940944848 5.3850992150679384E-5 64-65 0.0 0.0 0.0 0.4693652475853215 5.3850992150679384E-5 66-67 0.0 0.0 0.0 0.5360058503717873 5.3850992150679384E-5 68-69 0.0 0.0 0.0 0.6133089496040875 5.3850992150679384E-5 70-71 5.3850992150679384E-5 0.0 0.0 0.7052595187013726 5.3850992150679384E-5 72-73 5.3850992150679384E-5 0.0 0.0 0.798744841074952 5.3850992150679384E-5 74-75 5.3850992150679384E-5 0.0 0.0 0.903377318823722 1.0770198430135877E-4 76-77 5.3850992150679384E-5 0.0 0.0 1.0218225760591413 1.0770198430135877E-4 78-79 5.3850992150679384E-5 0.0 0.0 1.1545114207184153 1.0770198430135877E-4 80-81 5.3850992150679384E-5 0.0 0.0 1.3025208726445576 1.0770198430135877E-4 82-83 5.3850992150679384E-5 0.0 0.0 1.4617313309380413 1.0770198430135877E-4 84-85 5.3850992150679384E-5 0.0 0.0 1.6295579479756335 1.0770198430135877E-4 86-87 5.3850992150679384E-5 0.0 0.0 1.7983269573758627 1.0770198430135877E-4 88-89 5.3850992150679384E-5 0.0 0.0 1.9860784415092063 1.0770198430135877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5145 0.0 55.317177 1 GTATCAA 9605 0.0 43.679928 1 TATCAAC 12920 0.0 32.357895 2 ATCAACG 13000 0.0 32.231857 3 TCAACGC 13360 0.0 31.363333 4 CAACGCA 13790 0.0 30.38536 5 AACGCAG 14305 0.0 29.285925 6 TGGTATC 2135 0.0 27.592047 2 CGTACAC 160 6.236951E-8 26.722841 3 ACGCAGA 16955 0.0 24.541885 7 CGCAGAG 17010 0.0 24.29498 8 GCAGAGT 17765 0.0 22.781168 9 GTGGTAT 2715 0.0 22.75056 1 GAGTACT 11325 0.0 22.188046 12-13 AGTACTT 11700 0.0 21.761084 12-13 GTACTTT 11915 0.0 21.048351 14-15 GAGTACA 7890 0.0 20.9566 1 GGACCGA 365 0.0 20.82118 6 AGAGTAC 16505 0.0 20.476746 10-11 AGTACAT 7590 0.0 20.092009 2 >>END_MODULE