FastQCFastQC Report
Thu 26 May 2016
SRR937825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754722
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120240.6852367497529523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.4434320650222656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60640.3455818072606373No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22380.1275415706875505No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.11198355067070452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.056.682771
GTATCAA80250.049.4677541
ATCAACG101650.038.9844323
TCAACGC103250.038.4296264
TATCAAC103750.038.246612
CAACGCA106050.037.4160545
AACGCAG109250.036.3521846
ACGCAGA127400.031.024137
CGCAGAG128700.030.6369328
GCAGAGT136750.028.1370539
GTACATG82850.027.6589241
GTGGTAT18000.027.2047961
TACATGG83300.026.8131262
TGGTATC16500.026.4971072
ACATGGG84400.025.446533
GGACCGA1951.5690603E-824.3618976
GAGTACT87400.023.2053312-13
CATGGGG32850.022.1322654
CAGAGTA131700.021.92781310-11
AGTACTT91500.021.85406712-13
Produced by FastQC (version 0.11.5)