##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937825_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1754722 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25976080541533 33.0 31.0 34.0 31.0 34.0 2 32.415672112163634 34.0 31.0 34.0 31.0 34.0 3 32.45574854592351 34.0 31.0 34.0 31.0 34.0 4 36.01656843648168 37.0 35.0 37.0 35.0 37.0 5 36.01520411780328 37.0 35.0 37.0 35.0 37.0 6 36.08501916542905 37.0 37.0 37.0 35.0 37.0 7 36.05047010295648 37.0 37.0 37.0 35.0 37.0 8 36.04084008748964 37.0 37.0 37.0 35.0 37.0 9 37.757260694286614 39.0 38.0 39.0 35.0 39.0 10-11 37.6726438717928 39.0 38.0 39.0 35.0 39.0 12-13 37.57358373577125 39.0 38.0 39.0 35.0 39.0 14-15 38.84012624221957 40.0 38.0 41.0 36.0 41.0 16-17 38.78432395558954 40.0 38.0 41.0 36.0 41.0 18-19 38.7385699843052 40.0 38.0 41.0 36.0 41.0 20-21 38.67217086239302 40.0 38.0 41.0 35.0 41.0 22-23 38.49392068943115 40.0 38.0 41.0 34.5 41.0 24-25 38.339001277695274 40.0 38.0 41.0 34.0 41.0 26-27 38.28418490222383 40.0 38.0 41.0 34.0 41.0 28-29 38.319635816955625 40.0 38.0 41.0 34.0 41.0 30-31 38.126534858513196 40.0 38.0 41.0 34.0 41.0 32-33 37.88393061692963 40.0 37.5 41.0 34.0 41.0 34-35 37.915568392030195 40.0 38.0 41.0 34.0 41.0 36-37 37.840257032168054 40.0 38.0 41.0 34.0 41.0 38-39 37.71852777818937 40.0 38.0 41.0 33.5 41.0 40-41 37.48690875249755 40.0 37.0 41.0 33.0 41.0 42-43 37.22324704426114 40.0 37.0 41.0 33.0 41.0 44-45 36.9589701388596 40.0 37.0 41.0 32.0 41.0 46-47 36.57389233166279 40.0 36.5 41.0 31.0 41.0 48-49 36.194703206547814 39.0 36.0 41.0 30.0 41.0 50-51 35.27441412371874 38.5 35.0 40.0 28.5 40.5 52-53 35.37888736791355 38.5 35.0 40.0 29.0 40.5 54-55 35.740100141218946 39.0 35.5 40.0 29.0 41.0 56-57 35.56104414260493 39.0 35.0 40.0 28.0 41.0 58-59 35.33862457984797 39.0 35.0 40.0 28.0 41.0 60-61 35.04062181929673 38.0 35.0 40.0 27.0 41.0 62-63 34.69992996041538 38.0 34.5 40.0 26.0 41.0 64-65 34.34294093309367 37.5 34.0 40.0 26.0 41.0 66-67 33.95437795844584 37.0 34.0 39.5 25.5 41.0 68-69 33.482794425555724 36.0 33.5 39.0 23.5 41.0 70-71 33.063424291711165 36.0 33.0 39.0 22.5 40.0 72-73 32.61066767271397 35.0 33.0 38.5 21.5 40.0 74-75 32.160888733372005 35.0 32.0 37.5 20.0 39.5 76-77 31.670182513241414 35.0 32.0 37.0 18.5 39.0 78-79 31.157664006036285 35.0 31.0 36.5 16.5 39.0 80-81 30.64864690817121 34.5 31.0 36.0 10.0 37.5 82-83 30.14706489119074 34.0 30.0 35.5 7.0 37.0 84-85 29.69193353705031 34.0 30.0 35.0 2.0 36.5 86-87 29.23744701439886 34.0 29.0 35.0 2.0 36.0 88-89 28.782405133120804 34.0 29.0 35.0 2.0 36.0 90-91 28.31059421378429 34.0 29.0 35.0 2.0 35.0 92-93 27.782761029952322 33.0 27.0 35.0 2.0 35.0 94-95 27.181668378238832 33.0 26.0 35.0 2.0 35.0 96-97 26.49490375113551 32.5 25.0 34.5 2.0 35.0 98-99 25.573330134346065 32.0 22.5 34.0 2.0 35.0 100-101 23.12924297979965 29.5 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16782746601664655 1101 2 0.1963781767215238 1101 3 0.15973156871856276 1101 4 0.12605371120120878 1101 5 0.06634294863134471 1101 6 0.03171345808861048 1101 7 0.10779372603201409 1101 8 0.08504905916668548 1101 9 0.16237513919224256 1101 10-11 0.1382141089287714 1101 12-13 0.24471764721852907 1101 14-15 0.36027213148877735 1101 16-17 0.382102204650721 1101 18-19 0.4188564923673752 1101 20-21 0.399191694200141 1101 22-23 0.3833004136223863 1101 24-25 0.41190304306952896 1101 26-27 0.375800800276167 1101 28-29 0.352552131920973 1101 30-31 0.3317264621152489 1101 32-33 0.48120389479323933 1101 34-35 0.578666851414603 1101 36-37 0.5378334762885828 1101 38-39 0.5884514432720138 1101 40-41 0.6711325057656055 1101 42-43 0.6184179442971072 1101 44-45 0.6775285807311633 1101 46-47 0.6585316794069414 1101 48-49 0.7086160975670381 1101 50-51 0.6693737675743634 1101 52-53 0.703593239437204 1101 54-55 0.8785903038968144 1101 56-57 0.7964277080809978 1101 58-59 0.8951160732218355 1101 60-61 0.858111937858979 1101 62-63 1.0039302597215993 1101 64-65 0.7711491351424442 1101 66-67 0.9793014730825362 1101 68-69 0.9460925899037136 1101 70-71 0.9857726485203671 1101 72-73 0.9576147899076446 1101 74-75 1.0004868814224324 1101 76-77 1.0599738780803563 1101 78-79 1.046431517552076 1101 80-81 1.0246044001432217 1101 82-83 1.0923338039204538 1101 84-85 1.364471593120438 1101 86-87 1.431526966517044 1101 88-89 1.2813843237026994 1101 90-91 1.3665167382265473 1101 92-93 1.532279482649784 1101 94-95 1.6122830052671162 1101 96-97 1.8072020244527103 1101 98-99 2.031160275358012 1101 100-101 2.1331832038988097 1102 1 0.21216151886486045 1102 2 0.13539070515214036 1102 3 0.14889895859665359 1102 4 0.13601542690435053 1102 5 0.11032427940237 1102 6 0.04747272992143081 1102 7 0.03264657367253676 1102 8 0.1751652571231901 1102 9 0.2744631322608626 1102 10-11 0.22049654988002487 1102 12-13 0.28058699624239125 1102 14-15 0.3556017835388232 1102 16-17 0.44314396179333926 1102 18-19 0.43209564365938746 1102 20-21 0.43753161811805796 1102 22-23 0.5280158248584073 1102 24-25 0.47528578730452864 1102 26-27 0.4019490966571482 1102 28-29 0.24610992790165653 1102 30-31 0.5034898614402863 1102 32-33 0.4983489243811192 1102 34-35 0.600564149191456 1102 36-37 0.6488210909500864 1102 38-39 0.6924430158615777 1102 40-41 0.6887166492984562 1102 42-43 0.5768922531800555 1102 44-45 0.7875979911415456 1102 46-47 0.7674178074128548 1102 48-49 0.7402872132062157 1102 50-51 0.657825696849649 1102 52-53 0.5857947975673028 1102 54-55 0.6632180604770852 1102 56-57 0.763702987271607 1102 58-59 0.7298257142680953 1102 60-61 0.8656455591413561 1102 62-63 0.9996484963115364 1102 64-65 0.8908816458868714 1102 66-67 0.8604875243195309 1102 68-69 0.8395837586157597 1102 70-71 0.9292960666190808 1102 72-73 0.8734962087518809 1102 74-75 0.9775685319328034 1102 76-77 0.8594278564348201 1102 78-79 0.9633326466204544 1102 80-81 0.9932771527562636 1102 82-83 1.1056227691569411 1102 84-85 1.082048081224837 1102 86-87 1.170246388625209 1102 88-89 1.3217577655189423 1102 90-91 1.2997686461811107 1102 92-93 1.3755885129788759 1102 94-95 1.526926945566224 1102 96-97 1.7920838053200079 1102 98-99 2.0121241045317753 1102 100-101 2.240272005437909 1103 1 0.2194370642892025 1103 2 0.22233775860008365 1103 3 0.1603914179507484 1103 4 0.13326528898588919 1103 5 0.13046958989740176 1103 6 0.0945883239317169 1103 7 0.1576145656746064 1103 8 0.16100586722470922 1103 9 0.11086920357190877 1103 10-11 0.14781455074859906 1103 12-13 0.22985877067274885 1103 14-15 0.316253911044015 1103 16-17 0.30980852889042865 1103 18-19 0.2923614742201792 1103 20-21 0.2688632027246598 1103 22-23 0.269764471664935 1103 24-25 0.3442914341327139 1103 26-27 0.23708665812995378 1103 28-29 0.2108852044677576 1103 30-31 0.2743202012466739 1103 32-33 0.20823639401265837 1103 34-35 0.36943338960833927 1103 36-37 0.36186265688066044 1103 38-39 0.41983364871538953 1103 40-41 0.5576908025829042 1103 42-43 0.5980502779407786 1103 44-45 0.5982352755248499 1103 46-47 0.7545149383331236 1103 48-49 0.8084656355836657 1103 50-51 0.7702954939149222 1103 52-53 0.7406760194881699 1103 54-55 0.7722882547546703 1103 56-57 0.780270470151919 1103 58-59 0.8835431960658084 1103 60-61 0.8885704432818002 1103 62-63 0.9261264605831911 1103 64-65 0.8913293552415453 1103 66-67 0.9224746917741058 1103 68-69 0.9772034765803426 1103 70-71 0.97242763787672 1103 72-73 0.9434360328210758 1103 74-75 0.8889159957273094 1103 76-77 1.011690819651605 1103 78-79 1.1626310384634841 1103 80-81 1.0366151319837655 1103 82-83 1.1347440974973537 1103 84-85 1.2092017430561093 1103 86-87 1.2830568885726912 1103 88-89 1.231761385487065 1103 90-91 1.5027229732061294 1103 92-93 1.469048198648256 1103 94-95 1.4963211260961629 1103 96-97 1.6705751822624713 1103 98-99 1.8212666087015386 1103 100-101 2.2432607606323316 1104 1 0.19678855737947032 1104 2 0.18890568483342918 1104 3 0.25929492564019796 1104 4 0.16697798960895227 1104 5 0.13217620338573255 1104 6 0.07070780113576802 1104 7 0.12345852946405245 1104 8 0.1917172316251694 1104 9 0.24740449847782742 1104 10-11 0.23490768979674215 1104 12-13 0.21200167025568106 1104 14-15 0.3213176261377342 1104 16-17 0.407442542839874 1104 18-19 0.37885661297691087 1104 20-21 0.3443258668296707 1104 22-23 0.4229225658667133 1104 24-25 0.3943637663905122 1104 26-27 0.3706295749344193 1104 28-29 0.2392020996487858 1104 30-31 0.3492098198263349 1104 32-33 0.3317082802291651 1104 34-35 0.4419026701084974 1104 36-37 0.4243775451866014 1104 38-39 0.3972804818093181 1104 40-41 0.5427978347628439 1104 42-43 0.49728101299849214 1104 44-45 0.6030648911464951 1104 46-47 0.5887267159954845 1104 48-49 0.7429947501172691 1104 50-51 0.8037698902335322 1104 52-53 0.7833559822004474 1104 54-55 0.7482303977237237 1104 56-57 0.8293394014316107 1104 58-59 0.7722058162211809 1104 60-61 0.9331498933153171 1104 62-63 0.9110683159782766 1104 64-65 0.803907965039862 1104 66-67 0.8618938435424113 1104 68-69 0.8492804096560462 1104 70-71 0.868231983004236 1104 72-73 0.7399471919891525 1104 74-75 0.9028505359937924 1104 76-77 0.9426598123035745 1104 78-79 0.8698338431963712 1104 80-81 0.9741206279739103 1104 82-83 0.9915806156255726 1104 84-85 1.055666676490869 1104 86-87 1.1534749238665931 1104 88-89 1.2117531476719812 1104 90-91 1.3903242388241104 1104 92-93 1.416159268564197 1104 94-95 1.6324542196443588 1104 96-97 1.7284218839462504 1104 98-99 1.708116306236974 1104 100-101 2.1338778965674337 1105 1 0.10137754035902447 1105 2 0.12217996779607887 1105 3 0.13774290242386655 1105 4 0.11751368944398166 1105 5 0.15562549017423777 1105 6 0.1351662483433742 1105 7 0.16188053049442885 1105 8 0.20495068410084372 1105 9 0.0828560735683439 1105 10-11 0.21567745985283437 1105 12-13 0.16457789290682712 1105 14-15 0.2173064474298414 1105 16-17 0.21138709958951551 1105 18-19 0.2068439648201732 1105 20-21 0.2705680879248362 1105 22-23 0.3205492938111618 1105 24-25 0.30798346998490445 1105 26-27 0.22659744816761673 1105 28-29 0.3002899885539634 1105 30-31 0.326444179290057 1105 32-33 0.4006417760511738 1105 34-35 0.4507796554468584 1105 36-37 0.37947993388225143 1105 38-39 0.34242533148727006 1105 40-41 0.5651630625073878 1105 42-43 0.6139441542698947 1105 44-45 0.6627777757450772 1105 46-47 0.6737219927670566 1105 48-49 0.6603707022744558 1105 50-51 0.7457905586645026 1105 52-53 0.7733901304609461 1105 54-55 0.8870149936693608 1105 56-57 0.8543146235114776 1105 58-59 0.87738378057783 1105 60-61 0.8027331034601204 1105 62-63 0.7960540982194146 1105 64-65 0.8924702006852883 1105 66-67 0.8912006947474893 1105 68-69 0.8804242722233155 1105 70-71 1.0436135870110093 1105 72-73 0.9365846259180373 1105 74-75 1.1487352379416507 1105 76-77 1.1520906252196568 1105 78-79 1.28459479335449 1105 80-81 1.156909668321255 1105 82-83 1.1676109123917477 1105 84-85 1.1377518997164948 1105 86-87 1.3272333437523862 1105 88-89 1.2437479124600124 1105 90-91 1.459426392760495 1105 92-93 1.6352619833589195 1105 94-95 1.6387672811536902 1105 96-97 1.6128921772831752 1105 98-99 1.8075744169295795 1105 100-101 2.0937786286478044 1106 1 0.04781285238008337 1106 2 0.13713991129407788 1106 3 0.10312839555634667 1106 4 -0.023504179422481286 1106 5 0.04606714460997807 1106 6 -0.12007619435154737 1106 7 -0.10635796765984651 1106 8 -0.08344044733445344 1106 9 -0.03946227152001569 1106 10-11 -0.21600746577197327 1106 12-13 -0.06701596325749648 1106 14-15 -0.14613749239509843 1106 16-17 -0.07354809724273537 1106 18-19 0.103026672654849 1106 20-21 0.18305961100207924 1106 22-23 0.08638099485239792 1106 24-25 0.004867504599943118 1106 26-27 0.17487267154196218 1106 28-29 0.1199102656776816 1106 30-31 0.20528285979956706 1106 32-33 0.2093834708300335 1106 34-35 0.22240432596901627 1106 36-37 0.23421914209711048 1106 38-39 0.33238335517182094 1106 40-41 0.2543422908829882 1106 42-43 0.32706534911852003 1106 44-45 0.26826300724213326 1106 46-47 0.28438977418736755 1106 48-49 0.24681260114841308 1106 50-51 0.268688525176465 1106 52-53 0.2847102698988877 1106 54-55 0.18347556070113313 1106 56-57 0.13917681335345122 1106 58-59 0.2562919529262402 1106 60-61 0.24602776291767015 1106 62-63 0.40973977230106584 1106 64-65 0.3911380935088644 1106 66-67 0.30834425028526624 1106 68-69 0.32232071261879724 1106 70-71 0.5416553772938357 1106 72-73 0.40897622135331346 1106 74-75 0.5248169581810203 1106 76-77 0.4431428614508093 1106 78-79 0.5464370679710946 1106 80-81 0.5263868247043924 1106 82-83 0.19375944754704477 1106 84-85 0.30068457429243267 1106 86-87 0.5441929487142652 1106 88-89 0.5026136650431106 1106 90-91 0.7459923494386906 1106 92-93 0.8205350673580583 1106 94-95 0.9347270743113683 1106 96-97 1.1552377188273972 1106 98-99 1.4707876276255902 1106 100-101 2.0231463935198732 1107 1 0.031296315222654414 1107 2 0.041000145313219605 1107 3 0.061010448809234674 1107 4 0.03046713509281318 1107 5 -0.026442390023412088 1107 6 0.01822574736062421 1107 7 0.02497312752750247 1107 8 0.05938360916866969 1107 9 0.011753397972320556 1107 10-11 0.13373815779993237 1107 12-13 0.07240709355104258 1107 14-15 0.38605298988611736 1107 16-17 0.37774144660701836 1107 18-19 0.30202766541624015 1107 20-21 0.2359305801129068 1107 22-23 0.30403615939318485 1107 24-25 0.37045786292046046 1107 26-27 0.2986967931931872 1107 28-29 0.32782997333605124 1107 30-31 0.30535198252962203 1107 32-33 0.2331135162364717 1107 34-35 0.3228901873374497 1107 36-37 0.2940129931924673 1107 38-39 0.27986337891201885 1107 40-41 0.2923300573850298 1107 42-43 0.2860486443093393 1107 44-45 0.3239368388050181 1107 46-47 0.2321226203643434 1107 48-49 0.42578025481873283 1107 50-51 0.4169023914411625 1107 52-53 0.3794024331803456 1107 54-55 0.40644851348191224 1107 56-57 0.44793546714271315 1107 58-59 0.5108633770576958 1107 60-61 0.48956554971125144 1107 62-63 0.6018656981960078 1107 64-65 0.5424376785334104 1107 66-67 0.5133527643647184 1107 68-69 0.4145602591880291 1107 70-71 0.6175998304711783 1107 72-73 0.6658462078634741 1107 74-75 0.7419005826386993 1107 76-77 0.7313934616799642 1107 78-79 0.8575921557088542 1107 80-81 0.9575269283795222 1107 82-83 0.966281563533375 1107 84-85 1.073892837222985 1107 86-87 1.219658892097577 1107 88-89 1.4649499953881993 1107 90-91 1.4751597507647212 1107 92-93 1.5080460872485482 1107 94-95 1.7443132284876306 1107 96-97 1.8771261649615738 1107 98-99 2.239861078221516 1107 100-101 2.575966260223275 1108 1 0.2082590547334604 1108 2 0.10252562501693063 1108 3 0.19678719168395276 1108 4 0.19490417492141887 1108 5 0.2101677700265654 1108 6 0.19970259306249005 1108 7 0.14006763016866586 1108 8 0.16786227324561764 1108 9 0.1811121010201333 1108 10-11 0.17606738884725104 1108 12-13 0.2642068699346751 1108 14-15 0.2427760892748978 1108 16-17 0.33993442173353117 1108 18-19 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-0.631431007918458 2316 74-75 -0.849953402381427 2316 76-77 -0.7088411992593073 2316 78-79 -0.7244395449117782 2316 80-81 -0.6248120463526874 2316 82-83 -0.6801314044828537 2316 84-85 -0.6087837195451193 2316 86-87 -0.7492036483566871 2316 88-89 -0.8275162306774568 2316 90-91 -0.604647096518999 2316 92-93 -0.29377941461502033 2316 94-95 -0.18799470421829056 2316 96-97 -0.08474405419932296 2316 98-99 -0.1061306201338823 2316 100-101 0.2301586117196912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6485.0 3 900.0 4 747.0 5 793.0 6 881.0 7 1045.0 8 1201.0 9 1453.0 10 1864.0 11 2692.0 12 4232.0 13 6177.0 14 6908.0 15 9903.0 16 13781.0 17 19553.0 18 16072.0 19 8918.0 20 8331.0 21 8288.0 22 8908.0 23 9698.0 24 10538.0 25 12115.0 26 13988.0 27 16591.0 28 20216.0 29 25070.0 30 31980.0 31 42381.0 32 57285.0 33 80922.0 34 119319.0 35 184759.0 36 284408.0 37 397268.0 38 291418.0 39 27632.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59666837932262 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20.82904119538802 26.999384444606868 31.130486323021543 21.041088036983574 20-21 21.437096859374844 26.103429523275285 31.392520336343637 21.066953281006235 22-23 21.102944114910112 26.085246313433775 31.362045637491935 21.449763934164178 24-25 20.807965012769678 26.203942029349562 31.568119738948813 21.419973218931947 26-27 20.672498542601986 26.54008858010893 31.21432515192054 21.573087725368545 28-29 20.54350783751242 26.59103686324762 31.490613515658033 21.374841783581925 30-31 20.71080621648521 26.384488136955902 31.38237825041013 21.522327396148757 32-33 20.531494416179303 26.411116418657066 31.300061016157432 21.7573281490062 34-35 20.97189643397963 26.59043983125755 31.14240898802495 21.295254746737864 36-37 20.974268914366164 26.3585052916321 31.0191494451451 21.648076348856634 38-39 20.668620036619977 26.586194828099067 31.100403482037997 21.64478165324296 40-41 20.976379642995692 26.527379055819424 31.020033928911424 21.47620737227346 42-43 20.847712339185424 26.443220151239355 30.855500412242577 21.85356709733264 44-45 20.828175813936493 26.783198057879932 30.696128092227866 21.692498035955712 46-47 20.962182635768144 26.975438724702354 30.149866085982445 21.91251255354705 48-49 21.14545645048336 27.085403765276467 29.833490878869988 21.935648905370186 50-51 21.225880816492484 27.312856207565954 29.531397088866406 21.929865887075152 52-53 21.290707997833152 27.323957175913037 29.488834651061254 21.896500175192564 54-55 21.098713931382267 27.365671948584662 29.54023789186152 21.995376228171548 56-57 21.03907135010262 27.682625089656064 29.3832718691588 21.895031691082515 58-59 21.15337300737823 27.340554580354215 29.54828673773744 21.95778567453012 60-61 21.228244344971174 27.291911551883885 29.74622517584638 21.73361892729856 62-63 21.07901447503002 27.35374459009776 29.841821466731865 21.72541946814035 64-65 21.023478635403684 27.269377359616133 29.72724571519257 21.97989828978762 66-67 20.894423632226218 27.4943738503644 29.558066088846925 22.05313642856246 68-69 20.93286541053717 27.391324702224463 29.650154061696615 22.025655825541758 70-71 21.00253671268639 27.422500672926763 29.4769610712937 22.098001543093147 72-73 21.047739967208173 27.513764118068845 29.280806277031562 22.15768963769142 74-75 20.92135317682105 27.700168013879555 29.213406171698423 22.165072637600968 76-77 20.991273287031433 27.55018259636269 29.240512183368317 22.21803193323756 78-79 21.039732930662296 27.559183706098107 29.250864569317002 22.15021879392259 80-81 20.907280712898082 27.78872272426871 29.191652023974523 22.112344538858682 82-83 21.010712856283973 27.74885566049101 29.134681209308646 22.105750273916367 84-85 21.022582112035067 27.612782818766195 28.982713090511425 22.38192197868731 86-87 20.977861384951566 27.53089714842763 29.15688857615492 22.33435289046588 88-89 21.06327372175777 27.548626802228405 29.11735948649069 22.27073998952313 90-91 20.97922121002687 27.534501663884726 29.204895192533602 22.281381933554805 92-93 21.045394260188715 27.347060069505304 29.264336539499098 22.343209130806883 94-95 20.892786184405846 27.350661822134732 29.145180665110693 22.611371328348735 96-97 21.009770080985863 27.363562384631614 29.122770662412577 22.503896871969953 98-99 20.947505708321405 27.414643503589126 28.910410682289633 22.727440105799836 100-101 21.09922078752438 26.96176780958204 29.40929820069538 22.529713202198202 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2697.0 1 1639.0 2 669.0 3 745.0 4 606.5 5 534.5 6 1015.5 7 2392.0 8 3894.5 9 4238.0 10 3471.5 11 2788.5 12 2779.5 13 2949.0 14 3140.5 15 3337.0 16 3389.0 17 3548.0 18 3788.5 19 4008.0 20 4362.5 21 4794.0 22 5475.0 23 6497.0 24 7579.0 25 9135.0 26 11507.5 27 14187.0 28 16454.0 29 19059.0 30 22412.5 31 25857.5 32 29954.0 33 34411.5 34 39183.5 35 43245.5 36 47753.0 37 51836.5 38 53845.5 39 55681.0 40 57675.5 41 60521.5 42 62673.5 43 64536.5 44 66599.5 45 68458.0 46 67806.0 47 66565.5 48 66946.0 49 65350.0 50 62206.0 51 58281.0 52 54615.5 53 52026.5 54 49199.0 55 44549.5 56 39100.5 57 34397.5 58 31224.5 59 27792.5 60 23261.0 61 19038.5 62 14915.0 63 11071.0 64 8307.5 65 6493.0 66 4797.5 67 3586.5 68 2601.5 69 1673.5 70 1214.5 71 915.0 72 698.0 73 569.0 74 421.5 75 335.5 76 248.0 77 162.5 78 109.5 79 70.0 80 45.5 81 30.0 82 22.5 83 21.0 84 14.5 85 10.0 86 11.0 87 8.5 88 5.5 89 5.5 90 4.0 91 4.5 92 4.0 93 1.5 94 2.0 95 3.0 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28511638880688794 2 0.2683615980195153 3 0.25622292306131683 4 0.24260253191103776 5 0.24020899036998455 6 0.19655535178791853 7 0.18857687998440778 8 0.20157039120726813 9 0.20208329296606528 10-11 0.20416339454340915 12-13 0.2020547984239099 14-15 0.19672631904085092 16-17 0.19649836270360777 18-19 0.19706825354671567 20-21 0.1846161386248078 22-23 0.17621024868896612 24-25 0.17732153583302654 26-27 0.18880483632165093 28-29 0.20290963468857173 30-31 0.19621341728205377 32-33 0.20211178750822065 34-35 0.19456073383704087 36-37 0.1813962553612481 38-39 0.1834478623964366 40-41 0.17772045942320208 42-43 0.18954569441769126 44-45 0.18430269866109844 46-47 0.18378979690230132 48-49 0.19242364317538618 50-51 0.20222576567684225 52-53 0.2160741131643645 54-55 0.1902295634294207 56-57 0.16461297003172012 58-59 0.1679468314639014 60-61 0.17387369623222368 62-63 0.18170969532495745 64-65 0.1698559657883129 66-67 0.1755263796772366 68-69 0.17957260466330277 70-71 0.17356025626851432 72-73 0.17472853249688555 74-75 0.17558336876154743 76-77 0.1890897817432049 78-79 0.19470320654781784 80-81 0.18689570199723946 82-83 0.1866392511178409 84-85 0.17874626294079632 86-87 0.17920217561528265 88-89 0.18490108404636177 90-91 0.18512904038360492 92-93 0.18242205887884236 94-95 0.18202313528866682 96-97 0.17076779113728555 98-99 0.17737852491733733 100-101 0.17196456190781217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1754722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.511686917092646 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62475035421286 41.22432714492143 2 13.502868295737327 14.721282580399391 3 4.532009038559663 7.4114237344619545 4 2.093037383911587 4.563799943102363 5 1.1272244157425426 3.0723452218130083 6 0.673133833423218 2.2016196468521314 7 0.44278838353390504 1.689599921360804 8 0.33050976039768776 1.441331566547365 9 0.2373774760610561 1.164586199058895 >10 1.2981583689963794 12.946440863818637 >50 0.09838913166525182 3.680358412380935 >100 0.03777136528613332 3.551506548996379 >500 0.0013562369548495569 0.5250790357598696 >1k 3.129777588114362E-4 0.33513384140939145 >5k 2.0865183920762412E-4 0.7873626394557992 >10k+ 1.0432591960381206E-4 0.6838026996617212 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12024 0.6852367497529523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7781 0.4434320650222656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6064 0.3455818072606373 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2238 0.1275415706875505 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.11198355067070452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.8494542155395554E-5 0.0 22-23 0.0 0.0 0.0 2.849454215539555E-4 0.0 24-25 0.0 0.0 0.0 6.268799274187023E-4 0.0 26-27 0.0 0.0 0.0 8.548362646618666E-4 0.0 28-29 0.0 0.0 0.0 0.0017666616136345244 0.0 30-31 0.0 0.0 0.0 0.004046224986066169 0.0 32-33 0.0 0.0 0.0 0.007864493634889173 0.0 34-35 0.0 0.0 0.0 0.012252653126820088 0.0 36-37 0.0 0.0 0.0 0.018407474232385528 0.0 38-39 0.0 0.0 0.0 0.03006174197394231 0.0 40-41 0.0 0.0 0.0 0.04607567466527461 0.0 42-43 0.0 0.0 0.0 0.06134874926056663 0.0 44-45 0.0 0.0 0.0 0.07981321257726295 0.0 46-47 0.0 0.0 0.0 0.10853571106990167 0.0 48-49 0.0 0.0 0.0 0.1364888569243447 0.0 50-51 0.0 0.0 0.0 0.16694952248846257 0.0 52-53 0.0 0.0 0.0 0.19746717713689121 0.0 54-55 0.0 0.0 0.0 0.2315751440968997 0.0 56-57 0.0 0.0 0.0 0.2801298439296937 0.0 58-59 0.0 0.0 0.0 0.3305936780868992 0.0 60-61 0.0 0.0 0.0 0.38322309744791483 0.0 62-63 0.0 0.0 0.0 0.44086755622828 0.0 64-65 0.0 0.0 0.0 0.5081431702571689 0.0 66-67 0.0 0.0 0.0 0.5850214449924261 0.0 68-69 0.0 0.0 0.0 0.677942146961171 0.0 70-71 0.0 0.0 0.0 0.7812918513587908 0.0 72-73 0.0 0.0 0.0 0.8904259478139558 0.0 74-75 0.0 0.0 0.0 1.009305177686266 0.0 76-77 0.0 0.0 0.0 1.1433150094430913 0.0 78-79 0.0 0.0 0.0 1.2969290862028289 0.0 80-81 0.0 0.0 0.0 1.4704323533870323 2.8494542155395554E-5 82-83 0.0 0.0 0.0 1.6479248564729914 5.698908431079111E-5 84-85 0.0 0.0 0.0 1.8441952628393556 5.698908431079111E-5 86-87 0.0 0.0 0.0 2.047731777455346 5.698908431079111E-5 88-89 0.0 0.0 0.0 2.2675386756420677 5.698908431079111E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 56.68277 1 GTATCAA 8025 0.0 49.467754 1 ATCAACG 10165 0.0 38.984432 3 TCAACGC 10325 0.0 38.429626 4 TATCAAC 10375 0.0 38.24661 2 CAACGCA 10605 0.0 37.416054 5 AACGCAG 10925 0.0 36.352184 6 ACGCAGA 12740 0.0 31.02413 7 CGCAGAG 12870 0.0 30.636932 8 GCAGAGT 13675 0.0 28.137053 9 GTACATG 8285 0.0 27.658924 1 GTGGTAT 1800 0.0 27.204796 1 TACATGG 8330 0.0 26.813126 2 TGGTATC 1650 0.0 26.497107 2 ACATGGG 8440 0.0 25.44653 3 GGACCGA 195 1.5690603E-8 24.361897 6 GAGTACT 8740 0.0 23.20533 12-13 CATGGGG 3285 0.0 22.132265 4 CAGAGTA 13170 0.0 21.927813 10-11 AGTACTT 9150 0.0 21.854067 12-13 >>END_MODULE