FastQCFastQC Report
Thu 26 May 2016
SRR937825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754722
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110370.6289885235382016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87760.5001362039115028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.3736774258258573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.13375338087742672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.10035777747130314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55150.052.297261
GTATCAA95200.039.33011
TATCAAC126050.029.6187652
ATCAACG128100.029.033533
TCAACGC129450.028.767444
CAACGCA132750.028.0523175
AACGCAG136850.027.2450336
GCGTATC908.9323486E-426.3975261
TGGTATC24950.024.7491342
GTGGTAT26700.024.2026771
ACGCAGA159950.023.2548877
CGCAGAG161500.022.972878
GAGTACT101750.021.61639612-13
GCAGAGT169400.021.5930529
CGTAGAC1100.002878208621.5905933
AGTACTT104050.021.36684812-13
GTACTTT107150.020.36834714-15
AGAGTAC157850.019.77231410-11
CGTATCA1456.148416E-419.6548822
CAGAGTA166100.019.04764710-11
Produced by FastQC (version 0.11.5)