##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1754722 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.178009394080657 31.0 31.0 33.0 30.0 34.0 2 31.444805501954157 31.0 31.0 34.0 30.0 34.0 3 31.462036721486367 31.0 31.0 34.0 30.0 34.0 4 35.282030429891456 37.0 35.0 37.0 33.0 37.0 5 35.126120262924836 37.0 35.0 37.0 32.0 37.0 6 35.239912077240724 37.0 35.0 37.0 32.0 37.0 7 35.17759280387435 37.0 35.0 37.0 32.0 37.0 8 35.131971902101874 37.0 35.0 37.0 32.0 37.0 9 36.60517392498641 38.0 35.0 39.0 32.0 39.0 10-11 36.45932432601859 38.0 35.0 39.0 32.0 39.0 12-13 36.29320143019806 38.0 35.0 39.0 32.0 39.0 14-15 37.1280484885925 38.5 36.0 40.0 32.0 41.0 16-17 37.04380380481923 38.0 36.0 40.0 31.5 41.0 18-19 36.993190659261124 38.5 36.0 40.0 31.0 41.0 20-21 36.84461327777277 38.5 36.0 40.0 31.0 41.0 22-23 36.75596504745481 38.5 35.5 40.0 31.0 41.0 24-25 36.74203663030383 38.0 35.5 40.0 30.5 41.0 26-27 36.41546866113265 38.0 35.0 40.0 30.0 41.0 28-29 36.302775881307696 38.0 35.0 40.0 30.0 41.0 30-31 36.242531580501065 38.0 35.0 40.0 30.0 41.0 32-33 36.02077565563092 38.0 35.0 40.0 30.0 41.0 34-35 35.759352193680826 38.0 34.0 40.0 29.0 41.0 36-37 35.45512109610525 38.0 34.0 40.0 28.0 41.0 38-39 35.21244419343918 38.0 34.0 40.0 27.0 41.0 40-41 34.90074781076433 38.0 33.5 40.0 26.5 41.0 42-43 34.53165743633464 37.5 33.0 40.0 25.5 41.0 44-45 34.004888238706755 37.0 33.0 40.0 24.0 41.0 46-47 33.46173895352084 37.0 32.5 40.0 22.5 40.5 48-49 32.93219752188666 37.0 31.0 39.0 19.5 40.0 50-51 32.52421238236029 36.5 31.0 39.0 17.5 40.0 52-53 32.08765405574216 36.0 30.5 39.0 14.5 40.0 54-55 31.69465391098989 36.0 30.0 39.0 11.5 40.0 56-57 31.25526379677237 35.0 29.0 38.5 8.5 40.0 58-59 30.85261226564664 35.0 28.5 38.0 8.0 40.0 60-61 30.312909395334415 34.5 28.0 38.0 6.5 40.0 62-63 29.663108743151337 34.0 27.0 38.0 2.0 40.0 64-65 29.087665738504448 34.0 26.0 37.5 2.0 39.5 66-67 28.473439667366115 33.0 26.0 37.0 2.0 39.0 68-69 27.78313288372745 32.0 25.0 36.0 2.0 39.0 70-71 27.209643749836154 32.0 23.5 36.0 2.0 38.5 72-73 26.550383764493752 31.0 22.0 35.0 2.0 38.0 74-75 25.88896531758307 31.0 20.5 35.0 2.0 37.5 76-77 25.12927603346855 29.5 20.5 34.0 2.0 36.0 78-79 25.643406191977988 31.0 21.0 34.0 2.0 36.0 80-81 25.34251778914267 31.0 20.0 34.0 2.0 36.0 82-83 24.720673132268246 31.0 18.0 34.0 2.0 36.0 84-85 24.014519393955283 30.0 14.5 34.0 2.0 35.0 86-87 23.30148450865721 30.0 7.0 34.0 2.0 35.0 88-89 22.523195697096178 29.0 2.0 34.0 2.0 35.0 90-91 21.578599914972287 29.0 2.0 33.5 2.0 35.0 92-93 20.63873821608209 27.5 2.0 33.0 2.0 34.5 94-95 19.63973751967548 26.0 2.0 32.5 2.0 34.0 96-97 18.57649758765206 25.0 2.0 32.0 2.0 34.0 98-99 17.208553548653292 21.5 2.0 32.0 2.0 34.0 100-101 14.98894639720708 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22687836825915397 1101 2 0.2404575535105522 1101 3 0.2419281023104496 1101 4 0.2614563297575856 1101 5 0.16362703454806393 1101 6 0.14010065291139284 1101 7 0.06253133556754165 1101 8 0.1919687075065255 1101 9 0.2778625242167223 1101 10-11 0.19939363203729954 1101 12-13 0.28413566153967906 1101 14-15 0.2484601401336164 1101 16-17 0.41700625118210866 1101 18-19 0.3575634721066976 1101 20-21 0.3146348711324265 1101 22-23 0.2254115986051488 1101 24-25 0.2396526154226919 1101 26-27 0.141561730139955 1101 28-29 0.30560914561283425 1101 30-31 0.3989481455695554 1101 32-33 0.4747391432037844 1101 34-35 0.44499569642900383 1101 36-37 0.44042910588877504 1101 38-39 0.5346525760030545 1101 40-41 0.42813809463648766 1101 42-43 0.5788152867730147 1101 44-45 0.5053790559584215 1101 46-47 0.5833935272878179 1101 48-49 0.49661122411227865 1101 50-51 0.477519257097228 1101 52-53 0.27052775476889224 1101 54-55 0.3832379560134278 1101 56-57 0.6291705450540803 1101 58-59 0.7321633980248272 1101 60-61 0.7265930941904415 1101 62-63 0.6014561356803299 1101 64-65 0.8225852589712268 1101 66-67 0.7377264055688002 1101 68-69 1.000054511457062 1101 70-71 0.8410199747463807 1101 72-73 0.6633022847378633 1101 74-75 0.8873977994261679 1101 76-77 0.9147702910294804 1101 78-79 1.0199730291718474 1101 80-81 1.0633308167213045 1101 82-83 0.9725180963253734 1101 84-85 1.0734716192102596 1101 86-87 1.0671041355712916 1101 88-89 1.3269498846688528 1101 90-91 1.105776133781351 1101 92-93 1.159070145389915 1101 94-95 1.2856555336992095 1101 96-97 1.324738242740299 1101 98-99 1.299385176837145 1101 100-101 1.5302097607574847 1102 1 0.2173142697860193 1102 2 0.20754922762397143 1102 3 0.20114917319757097 1102 4 0.16875374172195023 1102 5 0.07223356055040853 1102 6 0.19828262459203216 1102 7 0.19363716578444468 1102 8 0.22396178041697112 1102 9 0.18816558617346146 1102 10-11 0.16874365754346599 1102 12-13 0.278807984160899 1102 14-15 0.41999432376803014 1102 16-17 0.41470148428274456 1102 18-19 0.3924511197509375 1102 20-21 0.34019640038654586 1102 22-23 0.19407648115156206 1102 24-25 0.3559775661811102 1102 26-27 0.3934091388489662 1102 28-29 0.38465430620154706 1102 30-31 0.3249572669767744 1102 32-33 0.361016859253219 1102 34-35 0.40557828884326597 1102 36-37 0.38579885950560566 1102 38-39 0.3491941875494433 1102 40-41 0.5027220006364033 1102 42-43 0.37944262051606614 1102 44-45 0.49539569757204305 1102 46-47 0.3429197063490079 1102 48-49 0.40878190654006374 1102 50-51 0.4108426216794996 1102 52-53 0.49108941280590557 1102 54-55 0.6415072496606378 1102 56-57 0.5901443155529194 1102 58-59 0.6896492086524191 1102 60-61 0.5566187041027213 1102 62-63 0.7209011206950073 1102 64-65 0.6979358346666018 1102 66-67 0.6097065357969385 1102 68-69 0.7398076847037025 1102 70-71 0.5295910639850696 1102 72-73 0.8649925961850187 1102 74-75 1.0373002105999483 1102 76-77 0.6254650318932242 1102 78-79 0.9933051295280784 1102 80-81 1.0396476398472778 1102 82-83 1.0428637031468568 1102 84-85 1.0065348244308296 1102 86-87 1.312010860972503 1102 88-89 1.350114799768722 1102 90-91 1.5328774057987609 1102 92-93 1.5817536270501549 1102 94-95 1.7855872966442128 1102 96-97 1.8601891367440935 1102 98-99 1.6664708410815017 1102 100-101 1.637137225930653 1103 1 0.20973953569620818 1103 2 0.22469585172186513 1103 3 0.1751457056868304 1103 4 0.07482621588158622 1103 5 0.1455920445800487 1103 6 0.09806873690552464 1103 7 0.18577093747450846 1103 8 -0.05590398551048992 1103 9 0.12300759147476015 1103 10-11 0.22685765594052043 1103 12-13 0.23311478889574033 1103 14-15 0.30300269800866886 1103 16-17 0.30751848693124373 1103 18-19 0.32585336906993234 1103 20-21 0.19363806315976717 1103 22-23 0.3171209512878974 1103 24-25 0.18058268456194781 1103 26-27 0.1712989533180007 1103 28-29 0.14789255468095774 1103 30-31 0.24166158572580088 1103 32-33 0.3121201800863602 1103 34-35 0.31915830490139285 1103 36-37 0.2893602603742238 1103 38-39 0.215023012623746 1103 40-41 0.2344446956892554 1103 42-43 0.48709811049387497 1103 44-45 0.551322236734002 1103 46-47 0.5934934346137624 1103 48-49 0.6658783771695553 1103 50-51 0.7039593856033548 1103 52-53 0.6740526761239636 1103 54-55 0.6329143436171485 1103 56-57 0.5852232704038833 1103 58-59 0.7091855843217232 1103 60-61 0.6394953959636283 1103 62-63 0.43306113581190786 1103 64-65 0.5302388877330451 1103 66-67 0.5098410454379874 1103 68-69 0.7283294511264629 1103 70-71 0.7718497809416789 1103 72-73 0.8909104406237311 1103 74-75 0.6749226126733703 1103 76-77 0.8426518460474988 1103 78-79 1.080345411023096 1103 80-81 1.2929295444622824 1103 82-83 1.1159963641526787 1103 84-85 1.2422113907470163 1103 86-87 1.4740560588602172 1103 88-89 1.5993553311721662 1103 90-91 1.4036301390715273 1103 92-93 1.8346575196700563 1103 94-95 2.049238808414838 1103 96-97 1.9997433814206573 1103 98-99 2.0001646204087464 1103 100-101 2.0928564087376085 1104 1 0.11619298445401682 1104 2 0.21093233844181825 1104 3 0.09491073965364905 1104 4 0.19101853175151717 1104 5 0.14392071511991844 1104 6 0.12243756871350797 1104 7 0.06993109624897897 1104 8 0.15690148413546723 1104 9 0.1554230099003675 1104 10-11 0.11817041740568612 1104 12-13 0.09532691098871027 1104 14-15 0.20460504993595663 1104 16-17 0.12736308363937354 1104 18-19 0.21386926772396464 1104 20-21 0.17769379583626232 1104 22-23 0.17503747147100057 1104 24-25 0.07360909803919924 1104 26-27 0.06503470373523612 1104 28-29 0.2499123916175492 1104 30-31 0.3006741228905767 1104 32-33 0.1325104909093966 1104 34-35 0.33839422470024516 1104 36-37 0.2258755183397767 1104 38-39 0.4751769972417321 1104 40-41 0.6381258801795653 1104 42-43 0.5361223425772224 1104 44-45 0.6926496694077784 1104 46-47 0.813149581703918 1104 48-49 0.9080144722367933 1104 50-51 0.8597561035305858 1104 52-53 0.9041399154266401 1104 54-55 1.0808437104772572 1104 56-57 0.8691210293562008 1104 58-59 0.6416884266970015 1104 60-61 0.8392667993374019 1104 62-63 0.865784092021137 1104 64-65 0.939637273113977 1104 66-67 1.052614885603532 1104 68-69 1.1416203229655508 1104 70-71 1.035357027970278 1104 72-73 0.982041315665807 1104 74-75 1.503516825690543 1104 76-77 1.2292331655860558 1104 78-79 1.4806534058537117 1104 80-81 1.5252328218366102 1104 82-83 1.2491341544986412 1104 84-85 1.024199790162946 1104 86-87 1.2772886425816274 1104 88-89 1.3344337979147767 1104 90-91 1.2799287238529224 1104 92-93 1.5492514142168794 1104 94-95 1.6480900299277828 1104 96-97 1.683826646608285 1104 98-99 1.5244063931514518 1104 100-101 1.5979549508125306 1105 1 0.1375420570747572 1105 2 0.1151493521596123 1105 3 0.14537497011194134 1105 4 0.10896095082967605 1105 5 0.14501338734549307 1105 6 0.11801379857568861 1105 7 0.08832985370739266 1105 8 0.09729205786950956 1105 9 0.28779899811273424 1105 10-11 0.2376920332866277 1105 12-13 0.12800732509446533 1105 14-15 0.100190353684809 1105 16-17 0.24585943044852598 1105 18-19 0.38394605323651376 1105 20-21 0.41040483681337037 1105 22-23 0.2723700292173774 1105 24-25 0.17916293763531854 1105 26-27 0.3561859816509525 1105 28-29 0.24147988651173335 1105 30-31 0.2227937347445561 1105 32-33 0.2889864617351492 1105 34-35 0.3904787348345522 1105 36-37 0.36472651590644745 1105 38-39 0.4872530784764564 1105 40-41 0.5679781260566585 1105 42-43 0.7682901521930603 1105 44-45 0.8044578806943292 1105 46-47 0.6954319266045559 1105 48-49 0.9421488114294974 1105 50-51 0.9842315006783835 1105 52-53 0.9942238799259115 1105 54-55 0.9571310806685993 1105 56-57 0.8898500530540616 1105 58-59 0.9692602426040651 1105 60-61 0.8680914125254233 1105 62-63 0.8683113597516723 1105 64-65 0.8887242558323258 1105 66-67 1.0534531685116946 1105 68-69 1.0476032392205887 1105 70-71 0.9958138110310735 1105 72-73 1.042719577677989 1105 74-75 1.1900951651812974 1105 76-77 1.1287216427515112 1105 78-79 1.1778874528392187 1105 80-81 1.0477325554288974 1105 82-83 1.1097132847996534 1105 84-85 1.4538115680277919 1105 86-87 1.6771624093943593 1105 88-89 1.6105216227218584 1105 90-91 1.6855192395781877 1105 92-93 1.7110914942754043 1105 94-95 2.0355799444930334 1105 96-97 2.0934231931631295 1105 98-99 2.111305984285721 1105 100-101 2.047918675296481 1106 1 -0.031624873678925525 1106 2 -0.08603141422991811 1106 3 -0.11800423684827521 1106 4 -0.04837398036529095 1106 5 -0.08711450143295707 1106 6 -0.09222064579785183 1106 7 0.04224270391110707 1106 8 0.10584766594202932 1106 9 -0.13803752679779535 1106 10-11 -0.2642084468289738 1106 12-13 -0.3980961890834962 1106 14-15 -0.2056485020414769 1106 16-17 -0.017931860412403466 1106 18-19 -0.2933585881693759 1106 20-21 -0.10503465445228244 1106 22-23 -0.2543272751482277 1106 24-25 -0.3292978965479634 1106 26-27 0.026647704897143853 1106 28-29 -0.05935434003057338 1106 30-31 -0.10030600145685042 1106 32-33 -0.2599708091662123 1106 34-35 -0.5994641466594217 1106 36-37 -0.2545072838424076 1106 38-39 -0.19942795885012288 1106 40-41 -0.41784986514789324 1106 42-43 -0.8348328975739108 1106 44-45 -0.7055982914247849 1106 46-47 -0.20052594673967405 1106 48-49 -0.17226274141296471 1106 50-51 -0.1604163027846397 1106 52-53 -0.36008593902757013 1106 54-55 -0.7363992291298054 1106 56-57 -0.49220404458494116 1106 58-59 -0.2728118742417287 1106 60-61 -0.5087667505754361 1106 62-63 -0.5690489129808114 1106 64-65 -0.865011133305746 1106 66-67 -0.6347915703882663 1106 68-69 -0.4054213592680078 1106 70-71 -0.3685628606938067 1106 72-73 -0.30283251199839256 1106 74-75 -0.26254763378773305 1106 76-77 -0.42212164206552316 1106 78-79 -0.06735627694190072 1106 80-81 -0.23245569985545345 1106 82-83 -0.2565848227510017 1106 84-85 0.03594375610677858 1106 86-87 0.4585235084578123 1106 88-89 0.14146536515752572 1106 90-91 -0.1581476473712975 1106 92-93 -0.1275819849349098 1106 94-95 0.13427525549740338 1106 96-97 0.002241383044037093 1106 98-99 0.12142394195031869 1106 100-101 0.4634099744856197 1107 1 0.0954303545755053 1107 2 0.1844862023537992 1107 3 0.1320201189774899 1107 4 0.19608350033981026 1107 5 0.13960357047022143 1107 6 0.06590424848591425 1107 7 0.05755370240433422 1107 8 0.02018718077398063 1107 9 0.08855054775314386 1107 10-11 0.09700398916600506 1107 12-13 0.08429783617842901 1107 14-15 0.09736083206725965 1107 16-17 0.11310816024373338 1107 18-19 0.23833626052864787 1107 20-21 0.1199256141730487 1107 22-23 0.22967632652554215 1107 24-25 0.06105725621834779 1107 26-27 0.25564745446546056 1107 28-29 0.2682448150762937 1107 30-31 0.18876253613569105 1107 32-33 0.29838649647579274 1107 34-35 0.43287695324369935 1107 36-37 0.48497847053970133 1107 38-39 0.6186864698996715 1107 40-41 0.5229908800268177 1107 42-43 0.5971861077401357 1107 44-45 0.6047409508609931 1107 46-47 0.6255326650320541 1107 48-49 0.829921662371369 1107 50-51 0.7700481135033428 1107 52-53 0.9516521943060852 1107 54-55 0.8410234583069531 1107 56-57 0.8585468918442629 1107 58-59 0.9891174000263874 1107 60-61 1.0046431487079914 1107 62-63 0.9906177797849409 1107 64-65 1.0961072563288923 1107 66-67 1.086857053044369 1107 68-69 1.49204180964308 1107 70-71 1.3343564315707255 1107 72-73 1.3253554862874388 1107 74-75 1.4221942930778475 1107 76-77 1.1565818928463862 1107 78-79 1.3806307364720318 1107 80-81 1.6607432570577458 1107 82-83 1.843512391360914 1107 84-85 1.70725471515253 1107 86-87 2.0423677662506314 1107 88-89 2.1415831098857616 1107 90-91 2.279271776683654 1107 92-93 2.3184230918352497 1107 94-95 2.139976760059863 1107 96-97 1.9628864030098363 1107 98-99 1.8292100661225952 1107 100-101 1.7848439678070438 1108 1 0.1897059120106377 1108 2 0.15624937818367712 1108 3 0.06991314577336993 1108 4 0.06974792686387588 1108 5 -0.020319931587174267 1108 6 0.13667166643308803 1108 7 0.12131278788501731 1108 8 0.010533422353546484 1108 9 0.1879342101163033 1108 10-11 0.10890414280989091 1108 12-13 0.3080470709432106 1108 14-15 0.16734312010491692 1108 16-17 0.13547803899408706 1108 18-19 0.2250224467327584 1108 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50-51 0.21015116044601712 2316 52-53 0.18234373933904635 2316 54-55 0.40462972376242234 2316 56-57 0.5186104860494716 2316 58-59 0.4492126654881474 2316 60-61 0.5545374380238748 2316 62-63 0.7463909674444764 2316 64-65 0.40139614642963295 2316 66-67 0.5852294440983954 2316 68-69 0.9701314875401117 2316 70-71 0.9754796705125273 2316 72-73 1.4119622152758673 2316 74-75 1.1445050153712657 2316 76-77 1.046207039404603 2316 78-79 0.9443041198221813 2316 80-81 0.9599013469663618 2316 82-83 0.8651138662766513 2316 84-85 1.19783880863098 2316 86-87 1.8047305678354526 2316 88-89 1.9156485456382022 2316 90-91 1.892902896734494 2316 92-93 2.2715905321055665 2316 94-95 1.7367953718520575 2316 96-97 2.1791355404400186 2316 98-99 2.124527753995018 2316 100-101 2.447779473802557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 14.0 4 55.0 5 107.0 6 252.0 7 708.0 8 1497.0 9 2566.0 10 3931.0 11 6146.0 12 8781.0 13 12003.0 14 16165.0 15 21339.0 16 28080.0 17 34479.0 18 30816.0 19 25116.0 20 24584.0 21 26161.0 22 28445.0 23 30843.0 24 35473.0 25 41297.0 26 48274.0 27 57048.0 28 67116.0 29 77913.0 30 90423.0 31 103953.0 32 120024.0 33 139101.0 34 157832.0 35 175650.0 36 174876.0 37 127213.0 38 35470.0 39 960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.620140190538734 23.40866180514701 16.448787584426718 19.522410419887535 2 18.059042970909353 24.029162454223517 36.940153483001865 20.971641091865266 3 21.732274400161394 26.55434878003467 30.220513562832174 21.49286325697176 4 15.788768819220367 19.575864438925368 35.62250886465206 29.0128578772022 5 13.509855192726809 38.958616397508735 33.522701366915 14.008827042849456 6 30.964961971184042 38.53721558172747 16.292837269949313 14.20498517713917 7 27.49774608171551 34.570091444684685 20.368525612604163 17.563636860995647 8 26.328501038911007 36.42759365871061 19.04142080625877 18.202484496119613 9 26.395406223891875 17.225634601948343 20.483358617490406 35.89560055666938 10-11 24.969567828978036 28.316451266924332 26.419256155675942 20.29472474842169 12-13 25.81793820443561 25.53839376214102 27.90731206762867 20.736355965794704 14-15 22.601699870406822 26.619829237907773 26.797521202788815 23.980949688896587 16-17 21.207319450032543 30.16232770775086 27.374364714182647 21.255988128033955 18-19 21.19538023686943 27.795229101817835 30.9227615542519 20.086629107060833 20-21 21.88968718068249 26.813637967104704 31.377559597861538 19.919115254351265 22-23 21.662394800243348 26.850867457211287 31.340558700288796 20.14617904225657 24-25 21.408861346697652 27.068333331433696 31.450679936764914 20.072125385103735 26-27 21.315074629289857 27.304395350447496 31.193250837105808 20.187279183156843 28-29 21.091762697153264 27.322818872458992 31.388825804149956 20.196592626237788 30-31 21.273461344150626 27.128607018264724 31.348507441209332 20.249424196375323 32-33 21.11405681355793 27.26306503252367 31.217138669259292 20.405739484659108 34-35 21.529849172689463 27.181741609212175 31.135216860562526 20.153192357535836 36-37 21.640390342257568 27.07685258558861 30.980161182732367 20.302595889421454 38-39 21.379967880952083 27.341766957956875 30.963394771365493 20.314870389725552 40-41 21.5474285746851 27.242989256105325 30.875824741019564 20.333757428190008 42-43 21.638881249225147 27.300437337102974 30.56369318287075 20.49698823080113 44-45 21.650523558717563 27.565534597503195 30.225072689577036 20.558869154202203 46-47 21.836421951739364 27.899490631564426 29.563486409813066 20.70060100688314 48-49 21.95379097087744 28.09442179444949 29.183369217460086 20.768418017212984 50-51 22.04072781899355 28.256156815723514 28.879104496324775 20.82401086895816 52-53 22.317473171668553 28.372508115960283 28.539942093374933 20.77007661899623 54-55 22.00322900151705 28.50756416116057 28.65812932190968 20.831077515412698 56-57 21.983624756514136 28.67260454932462 28.536771066869854 20.80699962729139 58-59 22.10390021895206 28.252167579821762 28.750622605746095 20.89330959548008 60-61 22.084979842960877 28.333405519506794 28.890616291355553 20.690998346176773 62-63 21.92538191234851 28.414016579264405 28.984790753179134 20.675810755207948 64-65 21.916890538786202 28.31579589245476 28.838414290126867 20.92889927863217 66-67 21.9227034253859 28.518278109011003 28.638525076906767 20.92049338869633 68-69 21.95570010520185 28.435387485880952 28.581051585379335 21.027860823537857 70-71 22.070162681040074 28.531926994703433 28.332607672326443 21.065302651930047 72-73 22.11344589057412 28.654054602381464 28.103910476987238 21.12858903005718 74-75 22.103615273530508 28.85534004816717 27.827826858043608 21.213217820258706 76-77 21.985163461790528 28.658157816451833 28.2526519870384 21.10402673471923 78-79 22.04135469892097 28.654966427730432 28.11274378505541 21.190935088293188 80-81 21.9269206176249 28.826389593337293 28.078179905420914 21.168509883616892 82-83 22.065163158649092 28.768090152352073 27.996285444075504 21.17046124492333 84-85 22.02168040058847 28.72207137498123 27.921629594718468 21.334618629711834 86-87 22.10335882265111 28.479981444354145 28.115080337512154 21.30157939548259 88-89 22.046027803834452 28.542014062626443 28.06447403064417 21.34748410289493 90-91 22.0795901315122 28.647819282148788 27.94779636982093 21.32479421651808 92-93 22.071681223522464 28.523458664485062 27.91251809811694 21.492342013875533 94-95 21.896317479350007 28.392075781804753 27.926503457527748 21.785103281317497 96-97 22.057539598865233 28.438322423722962 27.79730920339518 21.706828774016625 98-99 21.993225655429377 28.515875998777005 27.58922635868009 21.90167198711353 100-101 22.318433915305782 28.1617838377465 27.880016951041185 21.63976529590653 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 971.0 1 653.5 2 400.0 3 517.0 4 509.0 5 509.5 6 911.5 7 1829.5 8 2787.5 9 3121.5 10 2853.0 11 2672.0 12 2819.0 13 3158.5 14 3523.5 15 3802.0 16 4032.5 17 4277.0 18 4550.0 19 4746.5 20 5138.5 21 5724.0 22 6462.0 23 7518.5 24 8679.5 25 10258.5 26 12629.0 27 15186.0 28 17531.5 29 20340.5 30 23876.0 31 27349.0 32 31328.5 33 35774.0 34 40281.0 35 44148.0 36 48661.5 37 53164.0 38 55500.0 39 56963.0 40 58535.5 41 61418.5 42 63971.5 43 65383.5 44 66629.5 45 67502.5 46 66787.0 47 65490.5 48 65097.0 49 63712.0 50 60863.0 51 57178.5 52 53416.5 53 50051.5 54 46748.0 55 42491.5 56 37685.5 57 33256.0 58 29517.0 59 26123.0 60 22164.5 61 18132.5 62 14505.5 63 11100.0 64 8286.5 65 6332.0 66 4658.0 67 3551.5 68 2701.0 69 1806.0 70 1260.5 71 929.5 72 731.0 73 578.0 74 410.0 75 321.5 76 248.0 77 181.0 78 118.0 79 67.0 80 46.0 81 32.0 82 15.5 83 5.5 84 3.5 85 2.0 86 2.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030660127359205615 2 0.0 3 0.0 4 0.0 5 0.006496755611430187 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004644610371329475 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7096725293237335E-4 22-23 0.0013962325656143822 24-25 0.0 26-27 8.833308068172623E-4 28-29 0.0015671998185467556 30-31 2.8494542155395554E-5 32-33 0.0 34-35 0.0 36-37 3.134399637093511E-4 38-39 0.0 40-41 2.5645087939856E-4 42-43 0.02020263038817545 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.8494542155395554E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9946179508776888E-4 84-85 1.9946179508776888E-4 86-87 0.0 88-89 0.0 90-91 0.011853729536644552 92-93 0.002877948757694951 94-95 0.0 96-97 0.0 98-99 7.6935263819568E-4 100-101 0.014674689210028713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1754722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.669016808576735 #Duplication Level Percentage of deduplicated Percentage of total 1 77.2061320762314 43.75195596353153 2 12.857088036294485 14.57197076076246 3 4.2286706173194855 7.189038188724376 4 1.8615934678541626 4.219786860822568 5 1.02909986280618 2.915903871153372 6 0.6083749619681565 2.0685606567414414 7 0.40046321446215327 1.5885699642100701 8 0.2890366178127391 1.3103536762499426 9 0.2173901076784482 1.108735529944346 >10 1.1825523253523278 12.294381002220478 >50 0.08292359380097733 3.2251086992885023 >100 0.03476626136193666 3.451719603848747 >500 0.0012055939310770174 0.43223674523034 >1k 4.0186464369233916E-4 0.3703503724609275 >5k 2.0093232184616958E-4 0.8729565351181248 >10k+ 1.0046616092308479E-4 0.6283715696927374 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11037 0.6289885235382016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8776 0.5001362039115028 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6557 0.3736774258258573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2347 0.13375338087742672 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 0.10035777747130314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.698908431079111E-5 2 0.0 0.0 0.0 0.0 5.698908431079111E-5 3 0.0 0.0 0.0 0.0 5.698908431079111E-5 4 0.0 0.0 0.0 0.0 5.698908431079111E-5 5 0.0 0.0 0.0 0.0 5.698908431079111E-5 6 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 7 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 8 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 9 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 10-11 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 12-13 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 14-15 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 16-17 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 18-19 0.0 0.0 0.0 5.698908431079111E-5 5.698908431079111E-5 20-21 0.0 0.0 0.0 8.548362646618666E-5 5.698908431079111E-5 22-23 0.0 0.0 0.0 3.4193450586474664E-4 5.698908431079111E-5 24-25 0.0 0.0 0.0 6.838690117294933E-4 5.698908431079111E-5 26-27 0.0 0.0 0.0 9.118253489726577E-4 5.698908431079111E-5 28-29 0.0 0.0 0.0 0.001852145240100711 5.698908431079111E-5 30-31 0.0 0.0 0.0 0.004217192238998542 5.698908431079111E-5 32-33 5.698908431079111E-5 0.0 0.0 0.008177933598598525 5.698908431079111E-5 34-35 5.698908431079111E-5 0.0 0.0 0.012594587632684836 5.698908431079111E-5 36-37 5.698908431079111E-5 0.0 0.0 0.01872091419609488 5.698908431079111E-5 38-39 5.698908431079111E-5 0.0 0.0 0.03066012735920562 5.698908431079111E-5 40-41 5.698908431079111E-5 0.0 0.0 0.04687352184562569 5.698908431079111E-5 42-43 5.698908431079111E-5 0.0 0.0 0.061519716513499 5.698908431079111E-5 44-45 5.698908431079111E-5 0.0 0.0 0.07961375078217518 5.698908431079111E-5 46-47 5.698908431079111E-5 0.0 0.0 0.10773786388955059 5.698908431079111E-5 48-49 5.698908431079111E-5 0.0 0.0 0.1352635916116627 5.698908431079111E-5 50-51 5.698908431079111E-5 0.0 0.0 0.1646699591160309 5.698908431079111E-5 52-53 5.698908431079111E-5 0.0 0.0 0.19381987574100057 5.698908431079111E-5 54-55 5.698908431079111E-5 0.0 0.0 0.22704451189419178 5.698908431079111E-5 56-57 5.698908431079111E-5 0.0 0.0 0.2732626592702434 5.698908431079111E-5 58-59 5.698908431079111E-5 0.0 0.0 0.3211904791756187 5.698908431079111E-5 60-61 5.698908431079111E-5 0.0 0.0 0.3693462554182372 5.698908431079111E-5 62-63 5.698908431079111E-5 0.0 0.0 0.42200416932140816 5.698908431079111E-5 64-65 5.698908431079111E-5 0.0 0.0 0.4832104458711978 5.698908431079111E-5 66-67 5.698908431079111E-5 0.0 0.0 0.5533640086577817 5.698908431079111E-5 68-69 5.698908431079111E-5 0.0 0.0 0.6357132354868749 5.698908431079111E-5 70-71 5.698908431079111E-5 0.0 0.0 0.7286909265399306 1.1397816862158222E-4 72-73 5.698908431079111E-5 0.0 0.0 0.8267121515544913 1.1397816862158222E-4 74-75 5.698908431079111E-5 0.0 0.0 0.935247862624393 1.7096725293237332E-4 76-77 5.698908431079111E-5 0.0 0.0 1.0590566482895865 1.7096725293237332E-4 78-79 5.698908431079111E-5 0.0 0.0 1.199477752031376 1.7096725293237332E-4 80-81 5.698908431079111E-5 0.0 0.0 1.356368701138984 1.7096725293237332E-4 82-83 5.698908431079111E-5 0.0 0.0 1.5143994319328076 1.7096725293237332E-4 84-85 5.698908431079111E-5 0.0 0.0 1.6908091424168614 1.7096725293237332E-4 86-87 5.698908431079111E-5 0.0 0.0 1.8703247579958533 1.7096725293237332E-4 88-89 5.698908431079111E-5 0.0 0.0 2.0621500157859765 1.7096725293237332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5515 0.0 52.29726 1 GTATCAA 9520 0.0 39.3301 1 TATCAAC 12605 0.0 29.618765 2 ATCAACG 12810 0.0 29.03353 3 TCAACGC 12945 0.0 28.76744 4 CAACGCA 13275 0.0 28.052317 5 AACGCAG 13685 0.0 27.245033 6 GCGTATC 90 8.9323486E-4 26.397526 1 TGGTATC 2495 0.0 24.749134 2 GTGGTAT 2670 0.0 24.202677 1 ACGCAGA 15995 0.0 23.254887 7 CGCAGAG 16150 0.0 22.97287 8 GAGTACT 10175 0.0 21.616396 12-13 GCAGAGT 16940 0.0 21.593052 9 CGTAGAC 110 0.0028782086 21.590593 3 AGTACTT 10405 0.0 21.366848 12-13 GTACTTT 10715 0.0 20.368347 14-15 AGAGTAC 15785 0.0 19.772314 10-11 CGTATCA 145 6.148416E-4 19.654882 2 CAGAGTA 16610 0.0 19.047647 10-11 >>END_MODULE