FastQCFastQC Report
Thu 26 May 2016
SRR937824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439526
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105000.729406763059507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.4762678826224743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.3638697738005427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19270.13386350784911144No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.1227487381262999No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14850.10315895648984458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.056.677051
GTATCAA69150.049.7795261
ATCAACG90600.037.5059933
TATCAAC92600.036.7985732
TCAACGC92450.036.702784
CAACGCA93700.036.263875
AACGCAG97200.034.988696
ACGCAGA109650.030.715947
CGCAGAG110450.030.4074448
GTGGTAT14800.028.5912151
GCAGAGT117450.028.0289259
TGGTATC15250.027.7359472
GTACATG67750.026.8778081
TACATGG68200.025.9924552
ACATGGG69450.024.4297373
GAGTACT78100.023.38521212-13
AGTACTT80350.023.203312-13
AGAGTAC111700.022.9638210-11
CATGGGG26350.022.5443154
CAGAGTA114250.021.95236210-11
Produced by FastQC (version 0.11.5)