##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937824_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1439526 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25788766580111 33.0 31.0 34.0 31.0 34.0 2 32.385287935056404 34.0 31.0 34.0 31.0 34.0 3 32.440133071580505 34.0 31.0 34.0 31.0 34.0 4 36.01578505702572 37.0 35.0 37.0 35.0 37.0 5 36.025074225821555 37.0 37.0 37.0 35.0 37.0 6 36.10854614643987 37.0 37.0 37.0 35.0 37.0 7 36.04463205249506 37.0 37.0 37.0 35.0 37.0 8 36.05889438606875 37.0 37.0 37.0 35.0 37.0 9 37.71537506095756 39.0 38.0 39.0 35.0 39.0 10-11 37.66660900879873 39.0 38.0 39.0 35.0 39.0 12-13 37.58875317291942 39.0 38.0 39.0 35.0 39.0 14-15 38.828862417212335 40.0 38.0 41.0 36.0 41.0 16-17 38.75152272345203 40.0 38.0 41.0 36.0 41.0 18-19 38.702227677721694 40.0 38.0 41.0 35.5 41.0 20-21 38.722512827138935 40.0 38.0 41.0 35.5 41.0 22-23 38.72298346816939 40.0 38.0 41.0 35.0 41.0 24-25 38.66284874326688 40.0 38.0 41.0 35.0 41.0 26-27 38.55943727310239 40.0 38.0 41.0 35.0 41.0 28-29 38.40773455984817 40.0 38.0 41.0 35.0 41.0 30-31 38.15302919155333 40.0 38.0 41.0 34.0 41.0 32-33 37.99986349673434 40.0 38.0 41.0 34.0 41.0 34-35 37.97868291368131 40.0 38.0 41.0 34.0 41.0 36-37 37.829714086442344 40.0 38.0 41.0 34.0 41.0 38-39 37.67280132488055 40.0 37.5 41.0 33.0 41.0 40-41 37.43431205827474 40.0 37.0 41.0 33.0 41.0 42-43 37.14778996697524 40.0 37.0 41.0 33.0 41.0 44-45 36.84378364822865 40.0 37.0 41.0 31.5 41.0 46-47 36.411344775988766 39.5 36.0 41.0 30.5 41.0 48-49 36.01089282166491 39.0 36.0 41.0 29.5 41.0 50-51 35.147743771213584 38.5 35.0 39.5 28.0 40.5 52-53 35.260463861020924 38.5 35.0 40.0 28.5 40.5 54-55 35.53868252466437 39.0 35.0 40.0 28.0 41.0 56-57 35.31982645676424 39.0 35.0 40.0 27.5 41.0 58-59 35.05719174228183 38.5 35.0 40.0 27.0 41.0 60-61 34.72847937446076 38.0 34.5 40.0 26.0 41.0 62-63 34.379674281673275 38.0 34.0 40.0 26.0 41.0 64-65 33.952902899982355 37.0 34.0 40.0 24.5 41.0 66-67 33.44104135666879 36.5 33.5 39.0 22.0 41.0 68-69 33.005377117189965 36.0 33.0 39.0 21.5 40.0 70-71 32.554598874907434 35.5 32.0 39.0 20.0 40.0 72-73 32.08465355957447 35.0 32.0 38.0 18.5 40.0 74-75 31.542619931838676 35.0 31.0 37.0 15.0 39.0 76-77 31.009256171823225 35.0 31.0 37.0 9.5 39.0 78-79 30.489290224698962 34.0 30.5 36.0 7.0 38.5 80-81 29.954874034925382 34.0 30.0 36.0 2.0 37.5 82-83 29.42056135144485 34.0 29.0 35.0 2.0 37.0 84-85 28.8790021854416 34.0 29.0 35.0 2.0 36.5 86-87 28.298460048654903 33.5 28.0 35.0 2.0 36.0 88-89 27.758733430309697 33.0 26.5 35.0 2.0 35.5 90-91 27.208175816206168 33.0 26.0 35.0 2.0 35.0 92-93 26.547442699888713 32.0 25.0 34.5 2.0 35.0 94-95 25.78136101744602 32.0 23.5 34.0 2.0 35.0 96-97 24.891519152832252 31.5 19.0 34.0 2.0 35.0 98-99 23.672128186639213 31.0 2.0 34.0 2.0 35.0 100-101 20.333259003310815 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2142547959439085 1101 2 0.17519901853285091 1101 3 0.1833320534704228 1101 4 0.12949632083751084 1101 5 0.14479689630935155 1101 6 0.1753994202154061 1101 7 0.12997179850306395 1101 8 0.19808321288711994 1101 9 0.32962958471443926 1101 10-11 0.3290596538709707 1101 12-13 0.31626789235123454 1101 14-15 0.532404892849911 1101 16-17 0.5676192157632087 1101 18-19 0.565616017826521 1101 20-21 0.4805771363280158 1101 22-23 0.38658232237316525 1101 24-25 0.41785783278881183 1101 26-27 0.432813595915178 1101 28-29 0.4291595781234818 1101 30-31 0.5013137374600021 1101 32-33 0.5322482854240533 1101 34-35 0.617447195242768 1101 36-37 0.6728554899707504 1101 38-39 0.6080703725156269 1101 40-41 0.6041608824794764 1101 42-43 0.6507644098501189 1101 44-45 0.7330548029545056 1101 46-47 0.8378607071055626 1101 48-49 1.0676138368555783 1101 50-51 0.9855901525161386 1101 52-53 1.1005480360474351 1101 54-55 1.1931586255544673 1101 56-57 1.2975584197213337 1101 58-59 1.1971718307356412 1101 60-61 1.211894789991625 1101 62-63 1.2466731821229828 1101 64-65 1.1892435340846035 1101 66-67 1.2974295004298781 1101 68-69 1.3980679901259307 1101 70-71 1.4900572674761605 1101 72-73 1.445598476300983 1101 74-75 1.5334379779006788 1101 76-77 1.4042952688355577 1101 78-79 1.581659067871481 1101 80-81 1.781761051034369 1101 82-83 1.7463968094572415 1101 84-85 1.9456498778111069 1101 86-87 2.1918513761608516 1101 88-89 2.2238671021507486 1101 90-91 2.080586099517543 1101 92-93 2.1092745331238056 1101 94-95 2.472065093861282 1101 96-97 2.6669335137804424 1101 98-99 3.005159954871086 1101 100-101 3.8002139777003734 1102 1 0.15799779112118983 1102 2 0.16863932046194208 1102 3 0.05097890002659966 1102 4 0.1262976640653335 1102 5 0.05961230861746003 1102 6 0.13017501116772223 1102 7 0.1578246717539855 1102 8 0.11021556582829106 1102 9 0.18492225894348024 1102 10-11 0.27313495359247497 1102 12-13 0.24297027653817338 1102 14-15 0.37371594094119587 1102 16-17 0.3786276130597699 1102 18-19 0.2438086249790743 1102 20-21 0.2917970580438194 1102 22-23 0.2961400845658062 1102 24-25 0.2545558867216968 1102 26-27 0.29757434937938143 1102 28-29 0.3178297019178018 1102 30-31 0.33587118125160487 1102 32-33 0.4549216229461308 1102 34-35 0.4967713996647163 1102 36-37 0.5125532000305171 1102 38-39 0.6140372078640866 1102 40-41 0.7747421129508822 1102 42-43 0.7945850948793307 1102 44-45 0.7873291245480374 1102 46-47 0.7724473376584768 1102 48-49 0.8737011339279874 1102 50-51 0.7708618759572516 1102 52-53 0.7545597829228683 1102 54-55 0.9779806606040538 1102 56-57 1.0394599955941786 1102 58-59 1.0670762467456925 1102 60-61 1.0093793157014446 1102 62-63 0.9946459227492497 1102 64-65 1.0001338045981143 1102 66-67 1.1376386686814826 1102 68-69 1.1613587921569035 1102 70-71 1.1747409042102959 1102 72-73 1.1376724132660634 1102 74-75 1.170189735474377 1102 76-77 1.1694455328961517 1102 78-79 1.4437335779584295 1102 80-81 1.5545334112775429 1102 82-83 1.6267647324636236 1102 84-85 1.696226820481936 1102 86-87 1.8556292256378235 1102 88-89 1.9121299910245426 1102 90-91 1.9511256194536912 1102 92-93 2.3053691321932277 1102 94-95 2.2739138363894718 1102 96-97 2.2888858852313305 1102 98-99 2.4630702039880568 1102 100-101 3.096625491930773 1103 1 0.07966374037037127 1103 2 0.06331567108856007 1103 3 0.13399674608056955 1103 4 0.10025981970493802 1103 5 0.03732414671162587 1103 6 0.1484003555281177 1103 7 0.13537068056057677 1103 8 0.08301603438423655 1103 9 0.1483145616419108 1103 10-11 0.24559097354843828 1103 12-13 0.25592260238212816 1103 14-15 0.4175636743723814 1103 16-17 0.35746409126909384 1103 18-19 0.32732792821381196 1103 20-21 0.21400592179565336 1103 22-23 0.15924359732407822 1103 24-25 0.15171526630847154 1103 26-27 0.10148959650030065 1103 28-29 0.11691296586158018 1103 30-31 0.259568980308849 1103 32-33 0.3325793019006298 1103 34-35 0.3551165821475024 1103 36-37 0.4077071919295818 1103 38-39 0.3657396042737062 1103 40-41 0.38650840656045915 1103 42-43 0.43484403554772655 1103 44-45 0.6982801202294198 1103 46-47 0.7297207160597381 1103 48-49 0.732714925985114 1103 50-51 0.5809551461108029 1103 52-53 0.6310403385568293 1103 54-55 0.7534407486699877 1103 56-57 0.8201066592500368 1103 58-59 0.8319051779582978 1103 60-61 0.9830764091021464 1103 62-63 0.9505781126837647 1103 64-65 0.7372815937761672 1103 66-67 1.0731707689976062 1103 68-69 1.1215637324583625 1103 70-71 1.2479704119442943 1103 72-73 1.2346543202323126 1103 74-75 1.2715370102288084 1103 76-77 1.1479340342227822 1103 78-79 1.3839490325423718 1103 80-81 1.552579183689197 1103 82-83 1.6634213846878545 1103 84-85 1.71943762831755 1103 86-87 1.8069565655949198 1103 88-89 1.9030040877841685 1103 90-91 1.844313475810246 1103 92-93 2.1623259354279547 1103 94-95 2.337483347087222 1103 96-97 2.3232615026808077 1103 98-99 2.732370351470273 1103 100-101 3.4566086796636384 1104 1 0.22524511842953387 1104 2 0.27345758958583843 1104 3 0.2835606003378075 1104 4 0.2698790795672892 1104 5 0.21656912181038024 1104 6 0.28003286704035446 1104 7 0.3024209372795639 1104 8 0.3481068312149418 1104 9 0.4248067549005796 1104 10-11 0.3746311355855809 1104 12-13 0.3622072308853319 1104 14-15 0.452928946998604 1104 16-17 0.4255783740561867 1104 18-19 0.4745196365034374 1104 20-21 0.4539400262076967 1104 22-23 0.4122408809897138 1104 24-25 0.46430270172072596 1104 26-27 0.40762653112241054 1104 28-29 0.4593988923301495 1104 30-31 0.4988077946959919 1104 32-33 0.5407987746452605 1104 34-35 0.7157142754392609 1104 36-37 0.7507853899010328 1104 38-39 0.7388848175503782 1104 40-41 0.803483751020849 1104 42-43 0.875083432913172 1104 44-45 0.9233393247133677 1104 46-47 1.10031596591989 1104 48-49 1.0965267064987714 1104 50-51 0.8932398725325896 1104 52-53 0.9691992604368309 1104 54-55 1.0244983465636537 1104 56-57 1.1518919192375066 1104 58-59 1.1404974440212499 1104 60-61 1.1144084782580634 1104 62-63 1.1437936006855622 1104 64-65 1.231568995732907 1104 66-67 1.3260219680924124 1104 68-69 1.2561348218117985 1104 70-71 1.3486334477652804 1104 72-73 1.3949376540340772 1104 74-75 1.5246585054457604 1104 76-77 1.6310992890785947 1104 78-79 1.814108141193028 1104 80-81 1.9303406240717322 1104 82-83 1.8076254425069713 1104 84-85 2.0187910935931654 1104 86-87 2.1732028245152613 1104 88-89 2.2896685271050146 1104 90-91 2.2747239541879196 1104 92-93 2.389701866674592 1104 94-95 2.4484468751304753 1104 96-97 2.8211104668743303 1104 98-99 3.088175813514578 1104 100-101 3.699316917609451 1105 1 0.055733670955739 1105 2 0.10467908243574442 1105 3 0.04416660226110025 1105 4 0.015213448658876416 1105 5 -0.011683938215469425 1105 6 0.09917064977085488 1105 7 -0.031356164046862034 1105 8 0.002234743764127245 1105 9 0.09479387819315122 1105 10-11 0.08750335712280588 1105 12-13 0.10253272502561117 1105 14-15 0.0950601707711698 1105 16-17 0.09854260779404456 1105 18-19 0.11176344750877121 1105 20-21 0.08660332788569036 1105 22-23 0.03943176585949715 1105 24-25 0.061940403190462234 1105 26-27 0.05249010249303154 1105 28-29 0.04829940585413084 1105 30-31 0.11879331238350943 1105 32-33 0.19012678603107958 1105 34-35 0.2670696144682907 1105 36-37 0.32842619962605824 1105 38-39 0.12778295330808476 1105 40-41 0.32457073829048966 1105 42-43 0.3278882043301721 1105 44-45 0.310140246110052 1105 46-47 0.4363085570943568 1105 48-49 0.4734760765010222 1105 50-51 0.4780512409027651 1105 52-53 0.4748068667395273 1105 54-55 0.4136891681897552 1105 56-57 0.5248819407084895 1105 58-59 0.4648248854785635 1105 60-61 0.658129349089215 1105 62-63 0.7080070172194723 1105 64-65 0.6868922383491523 1105 66-67 0.839357805025692 1105 68-69 0.83736850990622 1105 70-71 0.9668257129651465 1105 72-73 0.905107507438661 1105 74-75 0.9767273949725315 1105 76-77 1.0043028192525725 1105 78-79 1.1979942153023622 1105 80-81 1.3736593337339684 1105 82-83 1.386087194420643 1105 84-85 1.5009269601382158 1105 86-87 1.6560876874345851 1105 88-89 1.7321894594149114 1105 90-91 1.740091175674884 1105 92-93 1.6586192647903744 1105 94-95 1.9238581980534413 1105 96-97 1.9746813202644518 1105 98-99 2.1530713057845112 1105 100-101 2.7866287400839553 1106 1 0.005176806958296254 1106 2 0.014984060280944789 1106 3 0.11629705613519548 1106 4 0.08604455320560334 1106 5 -0.005913709482086915 1106 6 0.0795386989505289 1106 7 0.1318744455096379 1106 8 0.10577552057942796 1106 9 0.14794544898872886 1106 10-11 -0.010191545276306613 1106 12-13 -0.168053479117944 1106 14-15 -0.2589452581538225 1106 16-17 -0.08038398196285357 1106 18-19 0.1254934383274957 1106 20-21 -0.0016666014584458821 1106 22-23 0.052945797235487646 1106 24-25 -0.22190056124210145 1106 26-27 0.050591176302454244 1106 28-29 0.251505465492464 1106 30-31 0.3305862547810179 1106 32-33 0.37471941706377976 1106 34-35 0.2151261790764849 1106 36-37 0.11508501564138385 1106 38-39 -0.07007761114044087 1106 40-41 0.2816213550323283 1106 42-43 0.4068800609426759 1106 44-45 0.6244324899326514 1106 46-47 0.6171376657127752 1106 48-49 0.742980687095411 1106 50-51 0.551474856726486 1106 52-53 0.7123263270405147 1106 54-55 0.5996322443144564 1106 56-57 0.5652631630149898 1106 58-59 0.4289279152977272 1106 60-61 0.6655819457919421 1106 62-63 0.6543157474098891 1106 64-65 0.9766489346886118 1106 66-67 1.036638951486907 1106 68-69 0.8661813317496652 1106 70-71 0.8981741621288535 1106 72-73 0.8630287481076984 1106 74-75 0.9893031404517494 1106 76-77 1.275908343982124 1106 78-79 1.358083832483313 1106 80-81 1.3014430549427018 1106 82-83 1.1859269213776535 1106 84-85 1.1929264811154212 1106 86-87 1.2790737957735736 1106 88-89 1.5099552172688888 1106 90-91 1.950669595698038 1106 92-93 2.103568368618564 1106 94-95 1.968374385032007 1106 96-97 2.358882350163455 1106 98-99 2.4640069969744793 1106 100-101 2.855451142383263 1107 1 0.15110440876824782 1107 2 0.06572233129784877 1107 3 0.03876431024331595 1107 4 0.13043369347709444 1107 5 0.07983289685275707 1107 6 0.1662140978550326 1107 7 0.11409402159918614 1107 8 0.07548259369207244 1107 9 0.1436587140518384 1107 10-11 0.1447608243355134 1107 12-13 0.13586888334931757 1107 14-15 0.23618691617339493 1107 16-17 0.14171294587710292 1107 18-19 0.24101499107815272 1107 20-21 0.1721545464339087 1107 22-23 0.06769097465868157 1107 24-25 0.22335426133470548 1107 26-27 0.24722847089640965 1107 28-29 0.13701749216548365 1107 30-31 0.15101969131352888 1107 32-33 0.21476585669226012 1107 34-35 0.36111689115078605 1107 36-37 0.22921099801813938 1107 38-39 0.29200740910247447 1107 40-41 0.3248340247155923 1107 42-43 0.33369596949233227 1107 44-45 0.34471350922295585 1107 46-47 0.4010028717142049 1107 48-49 0.41379159445430247 1107 50-51 0.3231669262668504 1107 52-53 0.5155771010343173 1107 54-55 0.530225242647397 1107 56-57 0.5911240822814818 1107 58-59 0.4770631856001799 1107 60-61 0.7541911384568678 1107 62-63 0.6840204390031204 1107 64-65 0.7986172604804693 1107 66-67 0.8975796516536079 1107 68-69 0.8403811412281144 1107 70-71 0.9182587060678884 1107 72-73 0.8099827847830987 1107 74-75 0.8833040930595182 1107 76-77 0.8401474485308249 1107 78-79 1.1563762830667876 1107 80-81 1.238102973971042 1107 82-83 1.2959912224017067 1107 84-85 1.3796149783766722 1107 86-87 1.4708575538112 1107 88-89 1.4307542170307386 1107 90-91 1.585570762642547 1107 92-93 1.668505258354216 1107 94-95 2.1255368046557237 1107 96-97 2.3169068798133736 1107 98-99 2.472104162966385 1107 100-101 2.9496271366676154 1108 1 -6.753004769279869E-4 1108 2 0.05195381875466154 1108 3 0.052963046257652024 1108 4 0.06154756382734661 1108 5 0.028045427247498367 1108 6 0.10246001685865735 1108 7 0.0983997144359634 1108 8 0.11153967726706782 1108 9 0.16435164613371 1108 10-11 0.11534157156288671 1108 12-13 0.12513405893767526 1108 14-15 0.29259996882676376 1108 16-17 0.2522044761928868 1108 18-19 0.23255994262354562 1108 20-21 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-2.1894487391509116 2316 82-83 -2.2580844011389836 2316 84-85 -2.532095113375064 2316 86-87 -2.574693567364047 2316 88-89 -2.400429981750605 2316 90-91 -2.3906867717584817 2316 92-93 -2.8972066984994456 2316 94-95 -2.7516530458072594 2316 96-97 -2.8488963354907995 2316 98-99 -2.8182680117800416 2316 100-101 -3.160971175814474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5367.0 3 783.0 4 723.0 5 699.0 6 798.0 7 910.0 8 959.0 9 1334.0 10 1667.0 11 2382.0 12 3719.0 13 5283.0 14 6006.0 15 8737.0 16 12058.0 17 17652.0 18 14306.0 19 8237.0 20 7348.0 21 7588.0 22 8061.0 23 9002.0 24 10116.0 25 11458.0 26 13397.0 27 15975.0 28 19473.0 29 23814.0 30 30194.0 31 39205.0 32 52962.0 33 73066.0 34 107158.0 35 162042.0 36 241158.0 37 315081.0 38 189295.0 39 11512.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59950114399571 19.05075187314924 15.182492231073894 23.167254751781154 2 14.395632794166099 19.9008225777353 42.14601608098027 23.55752854711833 3 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20.9933888680481 22-23 20.917739679569966 25.943821558119488 31.73751701351441 21.400921748796133 24-25 20.60166222029813 26.118929280119822 31.96502808763928 21.314380411942764 26-27 20.417197121933597 26.50002540616806 31.601201120098786 21.481576351799557 28-29 20.345738756599026 26.46215724054975 31.888845421335176 21.303258581516047 30-31 20.518434907969034 26.253685467554483 31.819398428670826 21.40848119580566 32-33 20.348555533744587 26.352651355366124 31.684180411057884 21.614612699831408 34-35 20.79800944878382 26.48704881778827 31.447068642553493 21.26787309087441 36-37 20.73441160625916 26.219669223841162 31.491144547872896 21.55477462202678 38-39 20.50661754112953 26.518707768242344 31.468414177273687 21.506260513354437 40-41 20.765368631772564 26.451002955261867 31.402501933812292 21.381126479153277 42-43 20.632000208799145 26.388905805486196 31.24289430413335 21.736199681581304 44-45 20.626089680012527 26.666666666666668 31.072123331767326 21.63512032155348 46-47 20.76157238309985 26.86377777182271 30.54019129447607 21.834458550601365 48-49 20.927465976509506 26.9811448153637 30.249112302056062 21.842276906070733 50-51 21.068148256939995 27.21472003185178 29.83754966834406 21.87958204286417 52-53 21.104691575116057 27.261389745426474 29.809074204986352 21.82484447447112 54-55 20.893337105918818 27.30112923506653 29.867566240371467 21.937967418643183 56-57 20.938244164635837 27.581150914326514 29.701172013633826 21.779432907403827 58-59 20.950700489234443 27.23010786953238 29.919662298473014 21.899529342760164 60-61 20.98229679272679 27.19778260187286 30.09197289212696 21.727947713273387 62-63 20.923469813789072 27.231469368441562 30.1701366660172 21.674924151752165 64-65 20.877605947205158 27.157766910059483 30.080916367862404 21.883710774872952 66-67 20.83325359509281 27.378446736189133 29.836147484313603 21.952152184404454 68-69 20.773255635937502 27.251139156648307 29.977947541731965 21.997657665682226 70-71 20.79959832887554 27.35890449280844 29.799585454801036 22.041911723514986 72-73 20.82448583600063 27.406293084789123 29.64842464089774 22.1207964383125 74-75 20.72258549469823 27.567207341390677 29.5154111436297 22.194796020281395 76-77 20.853716211866168 27.3230391459149 29.641823944130063 22.181420698088868 78-79 20.894304416365365 27.409965792394985 29.568490028291745 22.12723976294791 80-81 20.7640252065194 27.63881837393661 29.51483122287253 22.08232519667147 82-83 20.890864009208155 27.550353621849048 29.458721616827432 22.100060752115365 84-85 20.865907083179227 27.43007509431586 29.323142152430016 22.3808756700749 86-87 20.76941491508628 27.329247008325748 29.561207191709325 22.340130884878647 88-89 20.903107852154054 27.346704847130255 29.474399219687648 22.275788081028047 90-91 20.769010480369708 27.465848066478095 29.47124222601814 22.293899227134062 92-93 20.922479616747534 27.16710369918801 29.53466475151778 22.375751932546674 94-95 20.720174646840512 27.20307566614728 29.436580217779657 22.640169469232553 96-97 20.86677713880864 27.174685408420984 29.428344943115665 22.53019250965471 98-99 20.81880514621446 27.216009609830312 29.2317912228175 22.73339402113773 100-101 21.0805178358331 26.532789195169553 29.855337181266894 22.53135578773045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2303.0 1 1420.0 2 621.0 3 687.0 4 531.0 5 460.5 6 916.5 7 2085.0 8 3469.0 9 3722.5 10 2889.0 11 2312.5 12 2318.0 13 2515.0 14 2734.0 15 2897.0 16 2989.5 17 3119.0 18 3279.5 19 3458.0 20 3719.5 21 4099.0 22 4704.5 23 5544.5 24 6521.5 25 8026.0 26 9896.5 27 11876.0 28 13766.5 29 16053.5 30 18934.0 31 21716.0 32 25047.0 33 28839.0 34 32880.5 35 36114.5 36 39627.5 37 43023.0 38 44759.5 39 46036.5 40 47469.0 41 49783.5 42 51608.0 43 53234.0 44 54908.0 45 55914.5 46 55181.5 47 54312.5 48 54437.5 49 53206.5 50 50362.0 51 46858.0 52 43844.0 53 41804.0 54 39665.5 55 36033.5 56 31628.0 57 27729.0 58 24880.5 59 21998.5 60 18561.0 61 15151.5 62 11887.0 63 8808.5 64 6519.0 65 5071.5 66 3785.5 67 2813.0 68 2011.5 69 1350.0 70 990.0 71 739.0 72 575.0 73 446.5 74 348.5 75 260.0 76 180.0 77 121.5 78 74.0 79 50.0 80 35.0 81 26.5 82 14.0 83 8.5 84 10.5 85 10.5 86 9.0 87 9.0 88 9.5 89 8.0 90 5.5 91 4.5 92 2.5 93 1.5 94 2.5 95 2.5 96 1.5 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3225367238938373 2 0.27210345627657995 3 0.27849444886719654 4 0.2722423908981151 5 0.27231185820888265 6 0.20346975323821867 7 0.19485580670303976 8 0.20103839736135368 9 0.20333081861668356 10-11 0.20506750138587285 12-13 0.2084019323027163 14-15 0.20301821571822948 16-17 0.19509894229072625 18-19 0.1892289545308664 20-21 0.1880480102478177 22-23 0.19454320380458567 24-25 0.19263285275847744 26-27 0.19916277997062923 28-29 0.20492856676433768 30-31 0.2064221139458405 32-33 0.20423389365666197 34-35 0.20416442634589438 36-37 0.19339699317692075 38-39 0.1849914485740445 40-41 0.18044133971876855 42-43 0.1902362305369962 44-45 0.19110457192159086 46-47 0.19732189623528854 48-49 0.20458123021049984 50-51 0.1994753828690833 52-53 0.2101733487272894 54-55 0.1976692327891264 56-57 0.17450188464814112 58-59 0.15842020220544817 60-61 0.1755091606542709 62-63 0.16991704213748135 64-65 0.1770374414911575 66-67 0.17592596451887635 68-69 0.1735293422973951 70-71 0.17571756258657364 72-73 0.18610292554632565 74-75 0.19040989881391512 76-77 0.19176451137388278 78-79 0.18832587949088797 80-81 0.18585978995863917 82-83 0.17637750203886557 84-85 0.1713411220082166 86-87 0.176308034728098 88-89 0.18516511685096343 90-91 0.18071920896183882 92-93 0.17797525018651972 94-95 0.17846152136189272 96-97 0.1849914485740445 98-99 0.18506091588481208 100-101 0.1660963400452649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1439526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.198644303059055 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43871779495275 44.293896744384725 2 12.73486770946904 14.56834336720864 3 4.175335358715619 7.164705660874805 4 1.879867060334427 4.301033892844246 5 0.9924480167271276 2.838334054902569 6 0.5901723731561624 2.0254235789790944 7 0.41609922215063616 1.6660217981801655 8 0.29050953442214505 1.3293401220847652 9 0.20959617726814322 1.0789755471757292 >10 1.1572285273839866 12.191925917784877 >50 0.08254722761503457 3.198632747015266 >100 0.030792581143801016 2.992510674440502 >500 0.0010910499966910242 0.425058155059542 >1k 3.6368333223034134E-4 0.35911520147689524 >5k 2.4245555482022758E-4 0.8386057630163295 >10k+ 1.2122777741011379E-4 0.7280767745718092 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10500 0.729406763059507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6856 0.4762678826224743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5238 0.3638697738005427 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1927 0.13386350784911144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.1227487381262999 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1485 0.10315895648984458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.946731076757211E-5 2 0.0 0.0 0.0 0.0 6.946731076757211E-5 3 0.0 0.0 0.0 0.0 6.946731076757211E-5 4 0.0 0.0 0.0 0.0 6.946731076757211E-5 5 0.0 0.0 0.0 0.0 6.946731076757211E-5 6 0.0 0.0 0.0 0.0 6.946731076757211E-5 7 0.0 0.0 0.0 0.0 6.946731076757211E-5 8 0.0 0.0 0.0 0.0 6.946731076757211E-5 9 0.0 0.0 0.0 0.0 6.946731076757211E-5 10-11 0.0 0.0 0.0 3.4733655383786054E-5 6.946731076757211E-5 12-13 0.0 0.0 0.0 6.946731076757211E-5 6.946731076757211E-5 14-15 0.0 0.0 0.0 6.946731076757211E-5 6.946731076757211E-5 16-17 0.0 0.0 0.0 6.946731076757211E-5 6.946731076757211E-5 18-19 0.0 0.0 0.0 1.3893462153514422E-4 6.946731076757211E-5 20-21 0.0 0.0 0.0 2.4313558768650236E-4 6.946731076757211E-5 22-23 0.0 0.0 0.0 5.557384861405769E-4 6.946731076757211E-5 24-25 0.0 0.0 0.0 9.030750399784374E-4 6.946731076757211E-5 26-27 0.0 0.0 0.0 0.0012851452492000839 6.946731076757211E-5 28-29 0.0 0.0 0.0 0.0025702904984001677 6.946731076757211E-5 30-31 0.0 0.0 0.0 0.005071113686032764 6.946731076757211E-5 32-33 0.0 0.0 0.0 0.008857082122865442 6.946731076757211E-5 34-35 0.0 0.0 0.0 0.012434648627395406 6.946731076757211E-5 36-37 0.0 0.0 0.0 0.01854777197494175 6.946731076757211E-5 38-39 0.0 0.0 0.0 0.02806479355009913 6.946731076757211E-5 40-41 0.0 0.0 0.0 0.04008263831288911 6.946731076757211E-5 42-43 0.0 0.0 0.0 0.0547749745402306 6.946731076757211E-5 44-45 0.0 0.0 0.0 0.07217653588750741 6.946731076757211E-5 46-47 0.0 0.0 0.0 0.0991298524653254 6.946731076757211E-5 48-49 0.0 0.0 0.0 0.1262568373200623 6.946731076757211E-5 50-51 0.0 0.0 0.0 0.15574571074089666 6.946731076757211E-5 52-53 0.0 0.0 0.0 0.18641552844477974 6.946731076757211E-5 54-55 0.0 0.0 0.0 0.2223648617669983 6.946731076757211E-5 56-57 0.0 0.0 0.0 0.2743958775319098 6.946731076757211E-5 58-59 0.0 0.0 0.0 0.3291708520721404 6.946731076757211E-5 60-61 0.0 0.0 0.0 0.3827996159847061 6.946731076757211E-5 62-63 0.0 0.0 0.0 0.44274990517712076 6.946731076757211E-5 64-65 0.0 0.0 0.0 0.5078407753663359 6.946731076757211E-5 66-67 0.0 0.0 0.0 0.5815803257461136 6.946731076757211E-5 68-69 0.0 0.0 0.0 0.6746665221746602 6.946731076757211E-5 70-71 0.0 0.0 0.0 0.7829313260059214 6.946731076757211E-5 72-73 0.0 0.0 0.0 0.8983859965016263 6.946731076757211E-5 74-75 0.0 0.0 0.0 1.0246081001663048 6.946731076757211E-5 76-77 0.0 0.0 0.0 1.166634017030606 6.946731076757211E-5 78-79 0.0 0.0 0.0 1.3259572942760325 6.946731076757211E-5 80-81 0.0 0.0 0.0 1.51244229003158 6.946731076757211E-5 82-83 0.0 0.0 0.0 1.7121260748329659 6.946731076757211E-5 84-85 0.0 0.0 0.0 1.914241215511217 6.946731076757211E-5 86-87 0.0 0.0 0.0 2.123094685333922 6.946731076757211E-5 88-89 0.0 0.0 0.0 2.3516421377592347 6.946731076757211E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3280 0.0 56.67705 1 GTATCAA 6915 0.0 49.779526 1 ATCAACG 9060 0.0 37.505993 3 TATCAAC 9260 0.0 36.798573 2 TCAACGC 9245 0.0 36.70278 4 CAACGCA 9370 0.0 36.26387 5 AACGCAG 9720 0.0 34.98869 6 ACGCAGA 10965 0.0 30.71594 7 CGCAGAG 11045 0.0 30.407444 8 GTGGTAT 1480 0.0 28.591215 1 GCAGAGT 11745 0.0 28.028925 9 TGGTATC 1525 0.0 27.735947 2 GTACATG 6775 0.0 26.877808 1 TACATGG 6820 0.0 25.992455 2 ACATGGG 6945 0.0 24.429737 3 GAGTACT 7810 0.0 23.385212 12-13 AGTACTT 8035 0.0 23.2033 12-13 AGAGTAC 11170 0.0 22.96382 10-11 CATGGGG 2635 0.0 22.544315 4 CAGAGTA 11425 0.0 21.952362 10-11 >>END_MODULE