FastQCFastQC Report
Thu 26 May 2016
SRR937824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439526
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90180.6264562085019653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68460.47557320951479864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52990.36810727975736457No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.13365510591680874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45900.054.1430661
GTATCAA80350.040.095691
TATCAAC106800.029.9338022
ATCAACG107150.029.747363
TCAACGC109700.029.0558744
CAACGCA111450.028.5996365
AACGCAG115450.027.6891146
ACGCAGA133400.023.82097
CGCAGAG135000.023.397848
GAGTACT83450.022.71090512-13
GCAGAGT138350.022.2819619
TGGTATC20400.021.8884872
AGTACTT85650.021.71162212-13
GTACTTT88750.021.22084814-15
TAAGGTG5400.021.11235
AGAGTAC128950.020.14901710-11
CAGAGTA135650.019.78411110-11
GAGTACA66800.019.4195881
GTGGTAT23150.019.2943741
GTACACT6500.019.0010684
Produced by FastQC (version 0.11.5)