##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1439526 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.146100869313926 31.0 31.0 33.0 30.0 34.0 2 31.434621535144206 31.0 31.0 34.0 30.0 34.0 3 31.476527690364744 31.0 31.0 34.0 30.0 34.0 4 35.33187174111478 37.0 35.0 37.0 33.0 37.0 5 35.03146660775839 35.0 35.0 37.0 32.0 37.0 6 35.23402286586001 37.0 35.0 37.0 32.0 37.0 7 35.18009400316493 37.0 35.0 37.0 32.0 37.0 8 35.17501038536296 37.0 35.0 37.0 32.0 37.0 9 36.64965829029833 38.0 35.0 39.0 32.0 39.0 10-11 36.51910073176865 38.0 35.0 39.0 32.0 39.0 12-13 36.38330394866088 38.0 35.0 39.0 32.0 39.0 14-15 37.22379102565706 39.0 36.0 40.0 32.0 41.0 16-17 37.15471759454154 39.0 36.0 40.0 32.0 41.0 18-19 37.1208442918016 38.5 36.0 40.0 31.5 41.0 20-21 37.00625692068084 39.0 36.0 40.0 31.0 41.0 22-23 36.77809813785927 38.5 35.5 40.0 31.0 41.0 24-25 36.66583375361056 38.0 35.0 40.0 30.5 41.0 26-27 36.47195743598935 38.0 35.0 40.0 30.0 41.0 28-29 36.2632901385595 38.0 35.0 40.0 30.0 41.0 30-31 36.28483229896507 38.0 35.0 40.0 30.0 41.0 32-33 36.128894511109905 38.0 35.0 40.0 30.0 41.0 34-35 35.89376676767213 38.0 35.0 40.0 30.0 41.0 36-37 35.6125262760103 38.0 34.0 40.0 29.0 41.0 38-39 35.38084063782106 38.0 34.0 40.0 27.5 41.0 40-41 35.05616258407281 38.0 34.0 40.0 27.0 41.0 42-43 34.71715967617118 38.0 33.0 40.0 25.5 41.0 44-45 34.20777742117892 37.0 33.0 40.0 24.5 41.0 46-47 33.66788824932651 37.0 32.5 40.0 23.5 40.5 48-49 33.154302874696256 37.0 31.5 39.5 21.0 40.0 50-51 32.7632088618059 37.0 31.0 39.0 18.5 40.0 52-53 32.38300002917627 36.0 31.0 39.0 17.0 40.0 54-55 32.02373698008928 36.0 30.5 39.0 14.5 40.0 56-57 31.5860092141441 35.5 30.0 39.0 11.0 40.0 58-59 31.18661212093425 35.0 29.0 38.0 8.5 40.0 60-61 30.6351253815492 34.5 28.0 38.0 7.5 40.0 62-63 30.077116703692745 34.0 28.0 38.0 6.5 40.0 64-65 29.47338637857184 34.0 27.0 38.0 2.0 39.5 66-67 28.842487040873177 33.5 26.0 37.0 2.0 39.0 68-69 28.190565505590037 33.0 26.0 36.0 2.0 39.0 70-71 27.637356324234503 32.0 25.0 36.0 2.0 39.0 72-73 27.03522999931922 32.0 23.5 35.5 2.0 38.0 74-75 26.350585192625907 31.0 22.0 35.0 2.0 37.5 76-77 25.58852358345733 30.5 21.5 34.0 2.0 36.0 78-79 26.078082299312413 31.0 22.0 34.0 2.0 36.0 80-81 25.72890381972955 31.0 21.5 34.0 2.0 36.0 82-83 25.165640287150076 31.0 20.0 34.0 2.0 36.0 84-85 24.461952406556044 30.5 18.0 34.0 2.0 35.0 86-87 23.689934742408266 30.0 10.5 34.0 2.0 35.0 88-89 22.887920051461386 29.0 4.5 34.0 2.0 35.0 90-91 21.927611241478097 29.0 2.0 33.5 2.0 35.0 92-93 20.98809712363653 28.0 2.0 33.0 2.0 34.5 94-95 20.003639392411113 26.5 2.0 33.0 2.0 34.0 96-97 18.884994088331855 25.5 2.0 32.0 2.0 34.0 98-99 17.514738879325556 22.0 2.0 32.0 2.0 34.0 100-101 15.308034728097999 9.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06821895906266917 1101 2 0.09688195060230953 1101 3 0.25384666527857647 1101 4 0.19475013646321315 1101 5 -0.06730272083959221 1101 6 0.0802654008225332 1101 7 -0.003650016672459344 1101 8 0.053929504703909004 1101 9 0.22913076987479286 1101 10-11 0.17464189064633473 1101 12-13 0.26638294263788964 1101 14-15 0.319887966525755 1101 16-17 0.019634998308767138 1101 18-19 0.16629927504764197 1101 20-21 0.24280714943188997 1101 22-23 0.24239918692329354 1101 24-25 0.17408910488720863 1101 26-27 0.004232247291511726 1101 28-29 0.19770287455718716 1101 30-31 0.2980953185797759 1101 32-33 0.32548410010723927 1101 34-35 0.3252129319774326 1101 36-37 0.4843379833159531 1101 38-39 0.5931057717742334 1101 40-41 0.47729196054667256 1101 42-43 0.46882435359646024 1101 44-45 0.6623511146624352 1101 46-47 0.1686007114859862 1101 48-49 0.27969820378576316 1101 50-51 0.5399002449465726 1101 52-53 0.36820235361349063 1101 54-55 0.480132726749428 1101 56-57 0.6009549536369754 1101 58-59 0.6414223141944326 1101 60-61 0.652433041458405 1101 62-63 0.6979745683914764 1101 64-65 0.6087399815099701 1101 66-67 0.8639431701117779 1101 68-69 0.7336850953252956 1101 70-71 0.8220989301723485 1101 72-73 0.8625112959701156 1101 74-75 0.7613438967577544 1101 76-77 0.7033685478606024 1101 78-79 0.8999897066703646 1101 80-81 1.166402215345478 1101 82-83 1.0509664537844436 1101 84-85 1.2134993737920396 1101 86-87 1.4042653394909657 1101 88-89 1.6191065554076722 1101 90-91 1.5594023527028114 1101 92-93 1.7827749272049758 1101 94-95 1.6690082750572977 1101 96-97 1.8491488839266488 1101 98-99 2.2552897307275686 1101 100-101 2.334348788286599 1102 1 0.17394932857768097 1102 2 0.3484569743762549 1102 3 0.24410656542801235 1102 4 0.09038846685310631 1102 5 0.038907160630316184 1102 6 0.07519497051415414 1102 7 -0.006198074001623866 1102 8 0.08606041422707023 1102 9 0.27834410705724366 1102 10-11 0.1926177516027252 1102 12-13 0.1729183360966573 1102 14-15 0.10598105849695116 1102 16-17 0.1697108838881647 1102 18-19 0.33436152881750303 1102 20-21 0.3358833067135194 1102 22-23 0.36725484512190576 1102 24-25 0.08376817787321755 1102 26-27 0.23311514788245802 1102 28-29 0.06820227596325168 1102 30-31 0.3151608709843359 1102 32-33 0.3264400461403838 1102 34-35 0.5913134108525853 1102 36-37 0.5233878534083303 1102 38-39 0.5371464719168841 1102 40-41 0.456057158889287 1102 42-43 0.817542895574455 1102 44-45 0.8168855698994975 1102 46-47 0.8529403689713817 1102 48-49 0.6556291022710283 1102 50-51 0.7803469403683962 1102 52-53 0.34577694436203643 1102 54-55 0.5576614677492948 1102 56-57 0.8012992512867534 1102 58-59 0.9553328307682705 1102 60-61 1.123577098821535 1102 62-63 1.25765374751116 1102 64-65 1.159155282589989 1102 66-67 1.2699316567304422 1102 68-69 1.2451342657844755 1102 70-71 1.1465508558617934 1102 72-73 1.0931527254633906 1102 74-75 1.2734792214418995 1102 76-77 1.2187621764436756 1102 78-79 1.4485884237264735 1102 80-81 1.4981430836038676 1102 82-83 1.4462916898257454 1102 84-85 1.4855079960920037 1102 86-87 1.4763987749956478 1102 88-89 1.3607685875704227 1102 90-91 1.669973585381797 1102 92-93 1.994957375241924 1102 94-95 1.8455204676941683 1102 96-97 2.022454247498178 1102 98-99 2.2758186372959663 1102 100-101 2.6011178031962583 1103 1 0.030415259479791956 1103 2 0.25219483708371726 1103 3 0.12379741729658988 1103 4 0.23049304515275537 1103 5 -0.06739558719626615 1103 6 0.05163574476051025 1103 7 0.1193699795153691 1103 8 0.054572255143860104 1103 9 0.21071574813210248 1103 10-11 0.11379503369598609 1103 12-13 0.25821236553665017 1103 14-15 0.2956721220624985 1103 16-17 0.14601890649740312 1103 18-19 0.2322353856539081 1103 20-21 0.26031146029260555 1103 22-23 0.2999563806305474 1103 24-25 0.37532385064616136 1103 26-27 0.3388167645416047 1103 28-29 0.25845830579840623 1103 30-31 0.26834893903785684 1103 32-33 0.3350159783126756 1103 34-35 0.44474678276181123 1103 36-37 0.3120829709707138 1103 38-39 0.3465808352735422 1103 40-41 0.2524734821364376 1103 42-43 0.27955828594365073 1103 44-45 0.23622020634524432 1103 46-47 0.38339871053737085 1103 48-49 0.3570592034621711 1103 50-51 0.31004626011549163 1103 52-53 0.20187505179118403 1103 54-55 0.10646732046465601 1103 56-57 0.37728611196192574 1103 58-59 0.4145591136518618 1103 60-61 0.2680774763477842 1103 62-63 0.60578675671292 1103 64-65 0.5086047822794981 1103 66-67 0.7121630838502355 1103 68-69 0.43389077546608235 1103 70-71 0.5968285317114024 1103 72-73 0.6426571781561776 1103 74-75 0.6577003883155577 1103 76-77 0.5653595005940666 1103 78-79 0.8452516385565474 1103 80-81 0.7635136062131025 1103 82-83 0.8228891198246124 1103 84-85 0.8837509995073773 1103 86-87 0.9642919138970818 1103 88-89 1.3314437068263771 1103 90-91 1.5141509486255593 1103 92-93 1.5942855902131328 1103 94-95 1.563536584889608 1103 96-97 1.850060148903843 1103 98-99 1.8975492423258906 1103 100-101 2.332074338281503 1104 1 0.1529733011804204 1104 2 0.23337406709903163 1104 3 0.22616314868956522 1104 4 0.20146494160928086 1104 5 -0.004409677500781584 1104 6 0.03644680267120037 1104 7 0.2220183601865955 1104 8 0.06105754876818281 1104 9 0.36464695306574413 1104 10-11 0.3340852399324987 1104 12-13 0.31425047490990465 1104 14-15 0.272249744762199 1104 16-17 0.12217606856265206 1104 18-19 0.11293189059471587 1104 20-21 0.21639247705028453 1104 22-23 0.2121710000581345 1104 24-25 0.12484876412873547 1104 26-27 0.3383946039315333 1104 28-29 0.1640393937284017 1104 30-31 0.2869533587931059 1104 32-33 0.20578870562661677 1104 34-35 0.3864847652942274 1104 36-37 0.36928749027654106 1104 38-39 0.36067295309862857 1104 40-41 0.3692888928913405 1104 42-43 0.45819806972489374 1104 44-45 0.43360482786721377 1104 46-47 0.6277449316502555 1104 48-49 0.5913643486310249 1104 50-51 0.5456254803389413 1104 52-53 0.7104228691584993 1104 54-55 0.7363391105707073 1104 56-57 0.8348811269987841 1104 58-59 0.8055342377954133 1104 60-61 0.6076202917068585 1104 62-63 0.8850157973736152 1104 64-65 0.7296324379360648 1104 66-67 0.907629112897606 1104 68-69 0.8638073542628995 1104 70-71 0.9600576739173121 1104 72-73 1.162441040990359 1104 74-75 1.1993346043366735 1104 76-77 0.8639159039715345 1104 78-79 1.2340370807814018 1104 80-81 1.10073595486314 1104 82-83 1.3241690881280022 1104 84-85 1.379997566689962 1104 86-87 1.664058654576909 1104 88-89 1.9129565173892509 1104 90-91 2.140179433870223 1104 92-93 2.0906706487421616 1104 94-95 2.1841091684082734 1104 96-97 2.4257839758526707 1104 98-99 2.5701133132733496 1104 100-101 2.7109327162599737 1105 1 -0.0014366340585425519 1105 2 0.09788747439151635 1105 3 0.04349995732031786 1105 4 -0.021570269677312126 1105 5 -0.2598687959770274 1105 6 -0.14774257145904812 1105 7 -0.0646676930376131 1105 8 -0.01816619143854581 1105 9 -0.11554049335791206 1105 10-11 -7.830600062916915E-4 1105 12-13 -0.11347278447325948 1105 14-15 -0.03540661359316033 1105 16-17 -0.10894452855573888 1105 18-19 0.06650097904492469 1105 20-21 0.17510771355591004 1105 22-23 0.034126926076758934 1105 24-25 -0.017276453945527237 1105 26-27 -0.1725714334768611 1105 28-29 -0.22477172068652607 1105 30-31 -0.11597482359577782 1105 32-33 7.884837834737368E-4 1105 34-35 -0.11231335965322842 1105 36-37 0.07763141529208895 1105 38-39 -0.08721927967047094 1105 40-41 -0.23390542772812495 1105 42-43 0.04998450513213015 1105 44-45 0.03719000913756787 1105 46-47 0.29459511938348015 1105 48-49 0.23405018025722768 1105 50-51 0.3221355518949949 1105 52-53 0.2912740310405013 1105 54-55 0.18036367363164274 1105 56-57 0.36177452144152156 1105 58-59 0.4425503225262588 1105 60-61 0.42302069161282674 1105 62-63 0.5003225083860308 1105 64-65 0.47810925802422233 1105 66-67 0.8386770778360919 1105 68-69 0.6380872281383319 1105 70-71 0.748019631618071 1105 72-73 0.7505648583886924 1105 74-75 0.8841801929306499 1105 76-77 0.7724974095094019 1105 78-79 0.9834358368419487 1105 80-81 0.8446295691879833 1105 82-83 0.8460933187674051 1105 84-85 1.004554646439999 1105 86-87 0.9203506040731533 1105 88-89 1.358934525747717 1105 90-91 1.5442580138976254 1105 92-93 1.7434654188329972 1105 94-95 1.9153381132266958 1105 96-97 2.1438241620636376 1105 98-99 2.5186914213018916 1105 100-101 2.3966466798026875 1106 1 -0.16037759449924138 1106 2 0.15040833184231772 1106 3 0.08288201791669891 1106 4 0.10500950757708694 1106 5 0.0038520135714890102 1106 6 0.014514823455328951 1106 7 -0.18329649029121953 1106 8 -0.06544693871410345 1106 9 -0.06256935448121936 1106 10-11 -0.02430108323890323 1106 12-13 -0.21583237091692098 1106 14-15 0.023848705555778338 1106 16-17 0.2723388533451825 1106 18-19 0.33290790890800537 1106 20-21 0.20866964721125214 1106 22-23 0.26937022973729086 1106 24-25 0.14710740298632885 1106 26-27 -0.13269438266052447 1106 28-29 0.14586418207184693 1106 30-31 0.10610473410650911 1106 32-33 0.22528690700011822 1106 34-35 -0.27100842172659867 1106 36-37 -0.028475127360898966 1106 38-39 -0.12438845455370995 1106 40-41 0.04715161590286954 1106 42-43 0.1693146715925522 1106 44-45 0.32896418980899966 1106 46-47 0.7503505782473994 1106 48-49 0.59975594050632 1106 50-51 0.5924255602778956 1106 52-53 0.6387619556742479 1106 54-55 0.6961329044008728 1106 56-57 0.551720631377254 1106 58-59 0.6532103760171353 1106 60-61 0.6162658714459681 1106 62-63 0.7660488266966787 1106 64-65 0.5896353448071814 1106 66-67 0.822595683879765 1106 68-69 0.6723154026623028 1106 70-71 0.7388896567667693 1106 72-73 1.1431739358706317 1106 74-75 0.9702184748355656 1106 76-77 1.2367599140002383 1106 78-79 1.3530701827762464 1106 80-81 1.436756629983961 1106 82-83 1.340373986205833 1106 84-85 1.352414811702861 1106 86-87 1.618605364362164 1106 88-89 2.0147398828192884 1106 90-91 2.2935913738433342 1106 92-93 2.511880452165002 1106 94-95 2.042098521992809 1106 96-97 2.5060168889008914 1106 98-99 2.8036233601466485 1106 100-101 2.915236157268657 1107 1 0.15276124808232439 1107 2 0.2013014016208352 1107 3 0.17720447564472863 1107 4 0.07016787861780927 1107 5 -0.3175411752829973 1107 6 0.033616990637987954 1107 7 0.015377003397752276 1107 8 -0.027087805587171943 1107 9 0.027328240184175456 1107 10-11 0.005819183491276192 1107 12-13 0.09514405204092213 1107 14-15 0.1633533495186299 1107 16-17 0.15291279952284498 1107 18-19 0.21603722541193093 1107 20-21 0.05360534618720436 1107 22-23 0.09964648598877801 1107 24-25 0.29183948205277943 1107 26-27 -0.08188823834044712 1107 28-29 -0.19878930685408847 1107 30-31 -0.07279143164723934 1107 32-33 -0.1574149267698317 1107 34-35 0.26763887524887053 1107 36-37 0.09695354613278795 1107 38-39 -0.12090548241748422 1107 40-41 0.205138041242229 1107 42-43 0.2827857361317285 1107 44-45 0.2124447804258125 1107 46-47 0.39327270256271873 1107 48-49 0.4100190984010581 1107 50-51 0.601602745321955 1107 52-53 0.4280379737737974 1107 54-55 0.18849060861615996 1107 56-57 0.40167657326817974 1107 58-59 0.5146642936165655 1107 60-61 0.6682389602766392 1107 62-63 0.3158725942603873 1107 64-65 0.7106379644785328 1107 66-67 0.8470407898573775 1107 68-69 0.6411651287875699 1107 70-71 0.8662199735088514 1107 72-73 0.8418224236058052 1107 74-75 0.9325833236090801 1107 76-77 0.7986770371247935 1107 78-79 1.0264352697017038 1107 80-81 0.9867387685883884 1107 82-83 1.0055264034480338 1107 84-85 1.0178101439248195 1107 86-87 1.43778374016593 1107 88-89 1.632912066672585 1107 90-91 1.8634115691279263 1107 92-93 2.0251431337558543 1107 94-95 2.042598641068782 1107 96-97 2.581193121337183 1107 98-99 2.782893424447675 1107 100-101 2.7666436352396016 1108 1 0.05540821160318643 1108 2 0.07519691177601473 1108 3 0.00613754866563454 1108 4 0.08461508230139714 1108 5 -0.15612160120423368 1108 6 0.016476125126395402 1108 7 -0.1071264070762794 1108 8 -0.24098783581172967 1108 9 -0.02606167570441187 1108 10-11 -0.11437279692538738 1108 12-13 0.02816397574433438 1108 14-15 -0.12411523463771346 1108 16-17 0.07184971187978562 1108 18-19 0.1457378933474871 1108 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2316 58-59 -0.10314132236035789 2316 60-61 0.04345800404781386 2316 62-63 -0.12248508248973522 2316 64-65 -0.3940555621614159 2316 66-67 -0.06411670085234888 2316 68-69 -0.13872113288106291 2316 70-71 -0.018241777807759263 2316 72-73 0.14518747619980132 2316 74-75 0.021360648291850026 2316 76-77 0.1591855005532885 2316 78-79 0.06818982792623629 2316 80-81 -0.1654232462854459 2316 82-83 0.0384217275388572 2316 84-85 -0.047569430155434134 2316 86-87 -0.08481006694400861 2316 88-89 -0.607340191885978 2316 90-91 -0.5444908019474894 2316 92-93 -1.128145811404508 2316 94-95 -0.887874047167923 2316 96-97 -1.2207754538095088 2316 98-99 -1.2804912657433967 2316 100-101 -1.3539531738857722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 16.0 4 39.0 5 99.0 6 196.0 7 491.0 8 1091.0 9 1890.0 10 2957.0 11 4499.0 12 6552.0 13 9246.0 14 12110.0 15 16491.0 16 21382.0 17 27036.0 18 23924.0 19 18905.0 20 19006.0 21 20256.0 22 21755.0 23 24347.0 24 27869.0 25 32540.0 26 38541.0 27 45819.0 28 54153.0 29 62938.0 30 73833.0 31 85708.0 32 99391.0 33 115843.0 34 132470.0 35 149620.0 36 148664.0 37 108569.0 38 30535.0 39 740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.69117001228549 23.4001762217392 16.445879913501255 19.46277385247405 2 18.038159783150842 24.209566204431184 36.91590148423856 20.836372528179414 3 21.547578855817818 26.811394861919823 30.30448911655642 21.336537165705934 4 15.747416682586271 19.789236032719128 35.634184887376044 28.82916239731856 5 13.498076140107582 39.06033096782172 33.508789726074035 13.932803165996663 6 30.916148787864895 38.623546917527015 16.363997593652353 14.096306700955733 7 27.464109713891933 34.75164741727485 20.352741110615575 17.431501758217635 8 26.392784847234434 36.49784720803931 19.051826781871256 18.057541162854996 9 26.549294698393776 17.278326337975138 20.457289413320773 35.71508955031031 10-11 24.944634553318245 28.438979219548656 26.465447654297318 20.15093857283578 12-13 25.797430673870647 25.66213899786903 27.925313437294307 20.61511689096602 14-15 22.66357120329886 26.76898506869622 26.673085446181588 23.894358281823322 16-17 21.184785825334174 30.285698208993793 27.337852876571873 21.191663089100164 18-19 21.155852690399477 27.994701033534646 30.875684079342786 19.973762196723087 20-21 21.89100571369028 27.04072523922822 31.301575172365887 19.766693874715617 22-23 21.7046035152702 27.055555690631493 31.231706135276387 20.00813465882192 24-25 21.409472760655138 27.305478156909572 31.360240969714148 19.92480811272114 26-27 21.278622904993007 27.530993529670845 31.138615374569117 20.05176819076703 28-29 21.099366161763523 27.502146602532573 31.30180675853292 20.096680477170985 30-31 21.219845809500125 27.39885850680575 31.237365562639717 20.14393012105441 32-33 21.126190148701728 27.480990270408455 31.10527354142961 20.28754603946021 34-35 21.4859266175116 27.40065132550576 31.035875697972802 20.07754635900984 36-37 21.623070109932097 27.283583126378492 30.87890102638023 20.21444573730918 38-39 21.3516115721425 27.570394699366318 30.85227359561411 20.225720132877072 40-41 21.5360472822304 27.482657485866874 30.729698525764732 20.251596706137992 42-43 21.5947850185304 27.56154249903518 30.458843198671104 20.384829283763313 44-45 21.614406408776222 27.765736777244733 30.141206202597242 20.478650611381802 46-47 21.857715664739644 28.150099407721708 29.44469221118618 20.547492716352465 48-49 21.907488992904607 28.28569959834001 29.160397241869894 20.64641416688549 50-51 22.00099199319776 28.451344400865285 28.81302595437665 20.734637651560305 52-53 22.197376080737687 28.621087774725844 28.539463684573953 20.642072459962517 54-55 21.95861693362954 28.75522915181803 28.60559656442468 20.680557350127753 56-57 21.940694367451506 28.975197391363544 28.422828069795198 20.66128017138975 58-59 22.07549568399598 28.564263514517975 28.645852871014487 20.71438793047156 60-61 22.026625430870993 28.587257194382037 28.843452636492845 20.54266473825412 62-63 21.873762613526953 28.611813888738375 28.96220700425001 20.55221649348466 64-65 21.80637932208241 28.578261177637636 28.83598490058533 20.779374599694624 66-67 21.826524842205004 28.77916064037746 28.56422878086259 20.83008573655495 68-69 21.897346765532543 28.75477761429804 28.512718769928437 20.83515685024098 70-71 21.964591122355554 28.771102432328423 28.332381631175817 20.93192481414021 72-73 22.059027845315534 28.8554758378464 28.11988546213887 20.965610854699193 74-75 21.95054686460703 29.179809027216976 27.769516948131134 21.100127160044863 76-77 21.908843605464575 28.888988458700986 28.20397130722196 20.998196628612472 78-79 21.95097552944511 28.916844850318785 28.115365752337922 21.016813867898183 80-81 21.849483788413686 29.0727642293366 28.035061541090613 21.042690441159102 82-83 21.98446985563968 29.08244606025746 27.964332629628007 20.96875145447485 84-85 21.871967311394123 29.063411977439753 27.875970026182284 21.188650684983845 86-87 21.961360892404862 28.84984362908346 28.00196036750986 21.186835111001816 88-89 21.904814501440057 28.84320950090516 27.94919994498189 21.302776052672893 90-91 21.95852685833501 28.991564967259237 27.83970693619311 21.210201238212647 92-93 21.998752338496956 28.791699577559598 27.847478244076214 21.36206983986723 94-95 21.84510734783533 28.698995363751678 27.856426351451795 21.599470936961193 96-97 22.014885455351273 28.7938529766048 27.708808316070705 21.48245325197322 98-99 21.91147146158332 28.825316970985902 27.567714200070924 21.69549736735985 100-101 22.15202449444088 28.57997411326108 27.841062648548267 21.426938743749773 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 894.0 1 609.5 2 357.5 3 450.0 4 464.5 5 476.0 6 791.5 7 1517.0 8 2280.5 9 2563.0 10 2389.5 11 2285.5 12 2469.0 13 2700.5 14 3002.5 15 3216.5 16 3378.0 17 3597.0 18 3778.5 19 3961.0 20 4223.5 21 4718.5 22 5457.5 23 6270.0 24 7148.0 25 8449.5 26 10494.5 27 12838.5 28 14736.5 29 17047.0 30 20099.0 31 22925.0 32 26174.0 33 29655.5 34 33595.0 35 37116.5 36 40818.0 37 44296.5 38 46073.5 39 47257.5 40 48420.5 41 50598.0 42 52511.5 43 53553.5 44 54594.0 45 55474.5 46 54737.5 47 53736.0 48 53666.0 49 52192.5 50 49749.5 51 46769.5 52 43122.5 53 40074.0 54 37550.5 55 34125.0 56 30064.0 57 26461.0 58 23530.5 59 20978.0 60 17786.0 61 14484.5 62 11614.0 63 8854.5 64 6488.5 65 4837.5 66 3618.0 67 2738.0 68 2003.5 69 1353.5 70 953.0 71 708.5 72 559.5 73 433.5 74 308.5 75 240.5 76 182.5 77 121.5 78 76.5 79 50.5 80 32.0 81 18.5 82 11.0 83 6.0 84 3.0 85 2.0 86 1.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029870943630056004 2 0.0 3 0.0 4 6.946731076757211E-5 5 0.01611641609807673 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0030218280183893866 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.946731076757211E-5 22-23 4.515375199892187E-4 24-25 6.252057969081489E-4 26-27 0.0022229539445623074 28-29 0.0029176270522380285 30-31 5.557384861405769E-4 32-33 0.0 34-35 0.0 36-37 6.946731076757211E-5 38-39 0.0 40-41 0.0 42-43 0.009343353298238448 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3893462153514422E-4 74-75 1.0420096615135816E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0840193230271632E-4 84-85 2.0840193230271632E-4 86-87 0.0 88-89 0.0 90-91 0.007815072461351862 92-93 0.0022924212553298794 94-95 0.0 96-97 0.0 98-99 1.0420096615135816E-4 100-101 0.012434648627395408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1439526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.88943037441403 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82578981767985 46.42005861176452 2 11.995972518116659 14.128719767580304 3 3.915076745061684 6.916699181663925 4 1.7466560940568963 4.114383297560383 5 0.935998205504302 2.756020057681104 6 0.5479510326995479 1.9361114513248976 7 0.3666393412877734 1.511382737290115 8 0.26724427035990017 1.2590290281856338 9 0.19276274070020039 1.021651919752111 >10 1.101838481311362 11.883295973391087 >50 0.07172987730717195 2.8436089186538656 >100 0.030806491683339782 3.1114588204222864 >500 8.262068937978564E-4 0.3089583187545279 >1k 3.5408866877050985E-4 0.31764668542366536 >5k 3.5408866877050985E-4 1.4709752305516477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9018 0.6264562085019653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6846 0.47557320951479864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5299 0.36810727975736457 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1924 0.13365510591680874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4733655383786054E-5 0.0 12-13 0.0 0.0 0.0 6.946731076757211E-5 0.0 14-15 0.0 0.0 0.0 6.946731076757211E-5 0.0 16-17 0.0 0.0 0.0 6.946731076757211E-5 0.0 18-19 0.0 0.0 0.0 1.3893462153514422E-4 0.0 20-21 0.0 0.0 0.0 2.4313558768650236E-4 0.0 22-23 0.0 0.0 0.0 5.557384861405769E-4 0.0 24-25 0.0 0.0 0.0 9.030750399784374E-4 0.0 26-27 0.0 0.0 0.0 0.001215677938432512 0.0 28-29 0.0 0.0 0.0 0.00263975780916774 0.0 30-31 0.0 0.0 0.0 0.0051405809968003355 0.0 32-33 0.0 0.0 0.0 0.009030750399784373 0.0 34-35 0.0 0.0 0.0 0.01292091980276841 0.0 36-37 0.0 0.0 0.0 0.01889510852877961 0.0 38-39 0.0 0.0 0.0 0.028689999347007278 0.0 40-41 0.0 0.0 0.0 0.04098571335286754 0.0 42-43 0.0 0.0 0.0 0.05623378806634961 0.0 44-45 0.0 0.0 0.0 0.07367008306901021 0.0 46-47 0.0 0.0 0.0 0.10062339964682819 0.0 48-49 0.0 0.0 0.0 0.12677784215081908 0.0 50-51 0.0 0.0 0.0 0.15546784149782636 0.0 52-53 0.0 0.0 0.0 0.18523458416173103 0.0 54-55 0.0 0.0 0.0 0.2196209029916792 0.0 56-57 0.0 0.0 0.0 0.2694636984674122 0.0 58-59 0.0 0.0 0.0 0.32114737767848583 0.0 60-61 0.0 0.0 0.0 0.3720669164711162 0.0 62-63 0.0 0.0 0.0 0.4277449660513252 0.0 64-65 0.0 0.0 0.0 0.48766052158835616 0.0 66-67 0.0 0.0 0.0 0.555877420762112 0.0 68-69 0.0 0.0 0.0 0.6401412687231769 0.0 70-71 0.0 0.0 0.0 0.7375691720746969 0.0 72-73 0.0 0.0 0.0 0.8420827411245091 0.0 74-75 0.0 0.0 0.0 0.957051140444841 0.0 76-77 0.0 0.0 0.0 1.0886222270386225 0.0 78-79 0.0 0.0 0.0 1.2359971268320267 0.0 80-81 0.0 0.0 0.0 1.4051500285510645 0.0 82-83 0.0 0.0 0.0 1.5837852181898764 0.0 84-85 0.0 0.0 0.0 1.764886497360937 0.0 86-87 0.0 0.0 0.0 1.9475855246796514 0.0 88-89 0.0 0.0 0.0 2.1456368276779996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4590 0.0 54.143066 1 GTATCAA 8035 0.0 40.09569 1 TATCAAC 10680 0.0 29.933802 2 ATCAACG 10715 0.0 29.74736 3 TCAACGC 10970 0.0 29.055874 4 CAACGCA 11145 0.0 28.599636 5 AACGCAG 11545 0.0 27.689114 6 ACGCAGA 13340 0.0 23.8209 7 CGCAGAG 13500 0.0 23.39784 8 GAGTACT 8345 0.0 22.710905 12-13 GCAGAGT 13835 0.0 22.281961 9 TGGTATC 2040 0.0 21.888487 2 AGTACTT 8565 0.0 21.711622 12-13 GTACTTT 8875 0.0 21.220848 14-15 TAAGGTG 540 0.0 21.1123 5 AGAGTAC 12895 0.0 20.149017 10-11 CAGAGTA 13565 0.0 19.784111 10-11 GAGTACA 6680 0.0 19.419588 1 GTGGTAT 2315 0.0 19.294374 1 GTACACT 650 0.0 19.001068 4 >>END_MODULE