FastQCFastQC Report
Thu 26 May 2016
SRR937823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710987
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125120.4615293249285223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93710.3456674635474091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66030.24356442874864395No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36010.13282985126819125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30600.11287401968360601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96000.034.4865761
GTATCAA148500.034.3704571
ATCAACG183200.027.6683353
TATCAAC185200.027.420892
TCAACGC185750.027.3156174
CAACGCA187100.027.093115
AACGCAG193050.026.3539246
GTACATG122050.025.839721
TACATGG122000.025.2561112
ACATGGG117500.024.5237053
ACGCAGA215450.023.4816577
CGCAGAG216050.023.3272028
TAGGTCG1852.5171903E-723.1337595
GAGTACT119450.023.0043912-13
GCAGAGT231100.021.376429
GTACTTT129600.021.18480114-15
AGAGTAC180800.021.13552910-11
AGTACTT127250.020.82907312-13
CATGGGG57800.020.5677874
TATCCCG1200.00473465119.8136375
Produced by FastQC (version 0.11.5)