##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2710987 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.664398243149083 33.0 31.0 34.0 30.0 34.0 2 31.799205971847154 33.0 31.0 34.0 30.0 34.0 3 31.81898659049269 33.0 31.0 34.0 30.0 34.0 4 35.41646972117535 37.0 35.0 37.0 33.0 37.0 5 35.40556041028599 37.0 35.0 37.0 33.0 37.0 6 35.47007676539946 37.0 35.0 37.0 33.0 37.0 7 35.411568554183404 37.0 35.0 37.0 33.0 37.0 8 35.39831212764945 37.0 35.0 37.0 33.0 37.0 9 37.022506931977176 39.0 37.0 39.0 33.0 39.0 10-11 36.89263135529606 39.0 37.0 39.0 33.0 39.0 12-13 36.77408246516859 39.0 37.0 39.0 33.0 39.0 14-15 37.90210539556257 40.0 38.0 41.0 33.0 41.0 16-17 37.81796316249395 40.0 38.0 41.0 33.0 41.0 18-19 37.764632770278865 40.0 38.0 41.0 32.5 41.0 20-21 37.65931669904725 40.0 37.0 41.0 32.0 41.0 22-23 37.578222064510086 40.0 37.0 41.0 32.0 41.0 24-25 37.61298043848974 40.0 37.0 41.0 32.0 41.0 26-27 37.516709412475976 40.0 37.0 41.0 32.0 41.0 28-29 37.39402125498942 40.0 37.0 41.0 32.0 41.0 30-31 37.146964555713474 39.5 36.5 41.0 31.0 41.0 32-33 36.99311339375659 39.0 36.0 41.0 31.0 41.0 34-35 36.97761331942942 40.0 36.0 41.0 31.0 41.0 36-37 36.82885458322006 39.5 36.0 41.0 30.5 41.0 38-39 36.6742463169318 39.0 36.0 41.0 30.0 41.0 40-41 36.44306390993391 39.0 35.5 41.0 30.0 41.0 42-43 36.16577615458871 39.0 35.0 41.0 30.0 41.0 44-45 35.913486859214004 39.0 35.0 41.0 28.5 41.0 46-47 35.549067184755955 39.0 35.0 40.0 27.0 41.0 48-49 35.20708011510199 39.0 35.0 40.0 26.0 41.0 50-51 34.31334215176982 38.0 34.0 39.5 24.0 40.5 52-53 34.3982341117829 38.0 34.0 39.5 24.0 40.5 54-55 34.68372293928374 38.0 34.0 40.0 24.0 41.0 56-57 34.44382728504416 38.0 34.0 40.0 23.0 41.0 58-59 34.18622885318152 38.0 34.0 40.0 23.0 41.0 60-61 33.85685379531513 38.0 33.5 40.0 22.0 41.0 62-63 33.47927360035293 37.0 33.0 40.0 20.5 41.0 64-65 33.082487300750614 37.0 32.5 39.5 19.0 41.0 66-67 32.6393322800884 36.0 32.0 39.0 17.5 41.0 68-69 32.1184649723514 35.5 31.0 39.0 13.5 40.0 70-71 31.65567116330694 35.0 31.0 38.5 9.5 40.0 72-73 31.19561252045842 35.0 30.5 37.5 7.5 40.0 74-75 30.65521358088401 35.0 30.0 37.0 4.5 39.0 76-77 30.157330522057094 34.0 29.5 36.5 2.0 39.0 78-79 29.58749709976477 34.0 29.0 36.0 2.0 38.5 80-81 29.02167845142747 34.0 29.0 35.5 2.0 37.0 82-83 28.506814492286388 34.0 27.5 35.0 2.0 37.0 84-85 27.989285267690327 33.5 26.5 35.0 2.0 36.0 86-87 27.449946274179847 33.0 26.0 35.0 2.0 36.0 88-89 26.947781564426535 33.0 25.5 35.0 2.0 35.5 90-91 26.3763952021902 32.0 24.5 35.0 2.0 35.0 92-93 25.731753970048548 32.0 23.0 34.5 2.0 35.0 94-95 24.98250083825559 31.5 19.0 34.0 2.0 35.0 96-97 24.13595454349283 31.0 8.5 34.0 2.0 35.0 98-99 23.071718344647174 31.0 2.0 34.0 2.0 35.0 100-101 20.214804239194066 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23000771759682337 1101 2 0.2753688929845559 1101 3 0.30081733358829865 1101 4 0.2036307543322451 1101 5 0.14343232799986794 1101 6 0.18924153742121064 1101 7 0.21073057386983862 1101 8 0.22324886018099477 1101 9 0.3222653136163416 1101 10-11 0.2926512822246252 1101 12-13 0.31740559812082836 1101 14-15 0.44647333896333663 1101 16-17 0.4054710626792044 1101 18-19 0.3610786170004161 1101 20-21 0.40956766780144704 1101 22-23 0.37966249352991355 1101 24-25 0.2544101861817083 1101 26-27 0.2811318769814122 1101 28-29 0.3469321434460042 1101 30-31 0.42007997451560186 1101 32-33 0.34769556810281443 1101 34-35 0.43891868073887963 1101 36-37 0.4401534703127723 1101 38-39 0.41205890202747497 1101 40-41 0.5081390051727439 1101 42-43 0.44578030667300084 1101 44-45 0.44884747891730825 1101 46-47 0.4529522426822865 1101 48-49 0.6564708022161767 1101 50-51 0.5782697560133485 1101 52-53 0.4989453496694196 1101 54-55 0.5858213739922888 1101 56-57 0.5993936503484605 1101 58-59 0.461955403389986 1101 60-61 0.4671723324203185 1101 62-63 0.4600873645686292 1101 64-65 0.5887322627294012 1101 66-67 0.6091126475271906 1101 68-69 0.4700414887601525 1101 70-71 0.5964987621551501 1101 72-73 0.6548441963092273 1101 74-75 0.5696431835377709 1101 76-77 0.5084613896432231 1101 78-79 0.7379391844345484 1101 80-81 0.6629979318296648 1101 82-83 0.7121128809760506 1101 84-85 0.7511217929909435 1101 86-87 0.8416165574435688 1101 88-89 0.6637955122392185 1101 90-91 0.7975437396289955 1101 92-93 0.8797198954266854 1101 94-95 0.8272785304156791 1101 96-97 1.1856396610635827 1101 98-99 1.1867238774029296 1101 100-101 1.3214128657265256 1102 1 0.2621508996942268 1102 2 0.37873583334985383 1102 3 0.3479196973554224 1102 4 0.15981794034261299 1102 5 0.14256782038752647 1102 6 0.20320589919688814 1102 7 0.26159811439130465 1102 8 0.2551059721215623 1102 9 0.41694399745715316 1102 10-11 0.31004739352108857 1102 12-13 0.4629265054027414 1102 14-15 0.5391852583475867 1102 16-17 0.6131801007997879 1102 18-19 0.5963032733178366 1102 20-21 0.6562186164594337 1102 22-23 0.47968526605093587 1102 24-25 0.6057506677562756 1102 26-27 0.49574431746057 1102 28-29 0.5747475055933435 1102 30-31 0.6330061303752643 1102 32-33 0.6663522129753758 1102 34-35 0.7266271868152288 1102 36-37 0.8498338865101545 1102 38-39 0.736176068447314 1102 40-41 0.7860096408036696 1102 42-43 0.7145010667303069 1102 44-45 0.8169931043057375 1102 46-47 0.8545915943182862 1102 48-49 0.840054526186492 1102 50-51 0.7999040236711821 1102 52-53 0.7812067638172735 1102 54-55 0.8308146321951426 1102 56-57 0.7915989325452344 1102 58-59 0.7222421700878243 1102 60-61 0.769431541283403 1102 62-63 0.8112587229058121 1102 64-65 0.7855611891772369 1102 66-67 0.847264960737256 1102 68-69 0.7777866647639797 1102 70-71 0.8195928743532619 1102 72-73 0.8301649778539257 1102 74-75 0.7211484317556796 1102 76-77 0.7612359231792318 1102 78-79 0.9558952787055865 1102 80-81 0.9747890214510981 1102 82-83 1.091179420945938 1102 84-85 1.0382219823164398 1102 86-87 1.067613073710497 1102 88-89 1.0278596258818098 1102 90-91 1.2701795098619648 1102 92-93 1.2681623012535717 1102 94-95 1.4243100963169404 1102 96-97 1.5364402591121369 1102 98-99 1.579961363954034 1102 100-101 1.6130897920222047 1103 1 0.3193495017508887 1103 2 0.3433965612688361 1103 3 0.3541720321347128 1103 4 0.29615021480359616 1103 5 0.3046642032381115 1103 6 0.18696703171435303 1103 7 0.3018888309663552 1103 8 0.3768293119057802 1103 9 0.3949258102838584 1103 10-11 0.3899121910295307 1103 12-13 0.45688848294565076 1103 14-15 0.45959569476492845 1103 16-17 0.5763989954803606 1103 18-19 0.4512158728801978 1103 20-21 0.48510609972410634 1103 22-23 0.5263410717677957 1103 24-25 0.47733023255604223 1103 26-27 0.4344658120776046 1103 28-29 0.4457995222367259 1103 30-31 0.6604878986797971 1103 32-33 0.6276198380892524 1103 34-35 0.6617035278413255 1103 36-37 0.5920511351877877 1103 38-39 0.7180239991676558 1103 40-41 0.6349386851156069 1103 42-43 0.7125395886786805 1103 44-45 0.6345520013362389 1103 46-47 0.7202303477819285 1103 48-49 0.7153564338954652 1103 50-51 0.5759819606457057 1103 52-53 0.7967477660981857 1103 54-55 0.746220671719243 1103 56-57 0.7216908984807731 1103 58-59 0.7251095688499518 1103 60-61 0.9252467421521473 1103 62-63 1.0363477145498692 1103 64-65 1.0146858912870655 1103 66-67 0.9795212835435123 1103 68-69 0.9053532095962211 1103 70-71 0.8337461812964477 1103 72-73 0.814412692865691 1103 74-75 0.8772048163133945 1103 76-77 0.7977877656856265 1103 78-79 0.901176811595878 1103 80-81 0.8494469384143599 1103 82-83 1.066373000164571 1103 84-85 1.1599970889983986 1103 86-87 1.1576679150087337 1103 88-89 1.1962022842819309 1103 90-91 1.2515380919179009 1103 92-93 0.8793483770382018 1103 94-95 1.0732856781478937 1103 96-97 1.5126907787569515 1103 98-99 1.588429796157314 1103 100-101 1.5553801071074211 1104 1 0.3435874745749814 1104 2 0.35735175774331296 1104 3 0.2564856568133749 1104 4 0.18671209508269726 1104 5 0.25839170966484915 1104 6 0.17778598140219515 1104 7 0.2508421912307668 1104 8 0.25306875369791726 1104 9 0.3089911793620814 1104 10-11 0.27496544112133847 1104 12-13 0.4387100033275857 1104 14-15 0.5725226136044412 1104 16-17 0.505888529083002 1104 18-19 0.5105254578967333 1104 20-21 0.6053778588133483 1104 22-23 0.4778651261196103 1104 24-25 0.30197217159386724 1104 26-27 0.3942528117103592 1104 28-29 0.45094091041153916 1104 30-31 0.48604794274884 1104 32-33 0.600443929165273 1104 34-35 0.5577740383077199 1104 36-37 0.5692821304136473 1104 38-39 0.589489294797481 1104 40-41 0.6171274475100219 1104 42-43 0.5316728975292193 1104 44-45 0.5208905984717589 1104 46-47 0.6790992423834652 1104 48-49 0.6958926072336951 1104 50-51 0.7127796102968276 1104 52-53 0.787015852767297 1104 54-55 0.9276382258874349 1104 56-57 0.9324878136478674 1104 58-59 0.950228922504003 1104 60-61 0.9414053907015401 1104 62-63 1.0765607149171146 1104 64-65 1.08739980975934 1104 66-67 1.1776483492798349 1104 68-69 1.0979716451452433 1104 70-71 0.9848662694345336 1104 72-73 1.0037259503023428 1104 74-75 0.9982110594276037 1104 76-77 0.9364216253991771 1104 78-79 0.9805010862929002 1104 80-81 1.2032846174448402 1104 82-83 1.1686499006419844 1104 84-85 1.3096482090865358 1104 86-87 1.3886631408625725 1104 88-89 1.414894902717478 1104 90-91 1.4531539567728409 1104 92-93 1.4015665187936186 1104 94-95 1.6131314365026448 1104 96-97 1.782430036928087 1104 98-99 1.5517055949086895 1104 100-101 1.4946015540409512 1105 1 0.2717314256715575 1105 2 0.3388759869722797 1105 3 0.27758749943560446 1105 4 0.24195675792927318 1105 5 0.27526832606405094 1105 6 0.250621342338917 1105 7 0.2711629159216713 1105 8 0.2473803886632453 1105 9 0.268070898081902 1105 10-11 0.3773768998493665 1105 12-13 0.3930898986733311 1105 14-15 0.3532786463803035 1105 16-17 0.5111596178098452 1105 18-19 0.46380216989544465 1105 20-21 0.4881280740093956 1105 22-23 0.5234274241607935 1105 24-25 0.509751970174257 1105 26-27 0.5470332612223601 1105 28-29 0.5109077420456458 1105 30-31 0.6210156973456762 1105 32-33 0.5065057540666089 1105 34-35 0.6230381902409903 1105 36-37 0.670237251108027 1105 38-39 0.7192024768426464 1105 40-41 0.7283434849104182 1105 42-43 0.5485208535068153 1105 44-45 0.6758001772704247 1105 46-47 0.676651448070082 1105 48-49 0.5628144973836413 1105 50-51 0.47218120012441744 1105 52-53 0.5744050729512296 1105 54-55 0.6552311196573442 1105 56-57 0.7610095965398287 1105 58-59 0.8015911183392106 1105 60-61 0.9362839190908971 1105 62-63 0.930424139750059 1105 64-65 0.8434641606639346 1105 66-67 0.8757987319693328 1105 68-69 1.0133855113486874 1105 70-71 0.776585156360909 1105 72-73 0.9739647741952275 1105 74-75 0.8448399137685421 1105 76-77 0.6866086467944257 1105 78-79 0.82523203779947 1105 80-81 0.9091135642763604 1105 82-83 0.9837731793164934 1105 84-85 0.8677283105930087 1105 86-87 0.9438276516826072 1105 88-89 0.9816411384868502 1105 90-91 0.9886166137007955 1105 92-93 1.1627542843882885 1105 94-95 1.6280445711366767 1105 96-97 1.5169762404423395 1105 98-99 1.6444822942773847 1105 100-101 1.5666023623619942 1106 1 0.27710980441323585 1106 2 0.2618172565033454 1106 3 0.2780465978036304 1106 4 0.37268760323897254 1106 5 0.3034372163591854 1106 6 0.30699789812918965 1106 7 0.29578671893124664 1106 8 0.47464597189912183 1106 9 0.46006345737952614 1106 10-11 0.29321584524664246 1106 12-13 0.28973408606404405 1106 14-15 0.37101157934677076 1106 16-17 0.34809051419940573 1106 18-19 0.3668078165985662 1106 20-21 0.3338542197648522 1106 22-23 0.461645605490169 1106 24-25 0.4189158941417759 1106 26-27 0.4652801465397971 1106 28-29 0.4285158705683756 1106 30-31 0.5044540856208855 1106 32-33 0.4730194913444663 1106 34-35 0.5606737095649947 1106 36-37 0.5620598716291028 1106 38-39 0.4980778512818276 1106 40-41 0.5342968740180041 1106 42-43 0.6701609824265944 1106 44-45 0.6202598732935414 1106 46-47 0.7175466175073666 1106 48-49 0.6629597234327207 1106 50-51 0.6766084490601614 1106 52-53 0.5893852252188552 1106 54-55 0.6635140193805356 1106 56-57 0.5550494346973096 1106 58-59 0.4358639489184739 1106 60-61 0.4973907907801447 1106 62-63 0.5756545833486868 1106 64-65 0.6715579304716002 1106 66-67 0.7388059503605646 1106 68-69 0.6249500337984912 1106 70-71 0.2344160923528804 1106 72-73 0.6705023039358018 1106 74-75 0.7566299527815623 1106 76-77 0.7083806630944807 1106 78-79 0.8236104589353666 1106 80-81 0.8729975674900459 1106 82-83 0.644980039321478 1106 84-85 0.6855838765920161 1106 86-87 0.9313438467911404 1106 88-89 1.1850643682438715 1106 90-91 1.2332785611671184 1106 92-93 1.0988263876481987 1106 94-95 1.275728967892796 1106 96-97 1.4770481244931162 1106 98-99 1.5125295131649388 1106 100-101 1.6683153989978372 1107 1 0.29114940893936136 1107 2 0.3033429779997725 1107 3 0.238439103434942 1107 4 0.17081386265525822 1107 5 0.24410871458361783 1107 6 0.2012525667118581 1107 7 0.28268990263173777 1107 8 0.17351603594267573 1107 9 0.2706066199015993 1107 10-11 0.2684607902358209 1107 12-13 0.41385154389737266 1107 14-15 0.4561854089406765 1107 16-17 0.3830140281392076 1107 18-19 0.3919996671386201 1107 20-21 0.40715748850551137 1107 22-23 0.2890796483449307 1107 24-25 0.47863156613161806 1107 26-27 0.47567326437125246 1107 28-29 0.42791851163691774 1107 30-31 0.5565045479804098 1107 32-33 0.6013711480137189 1107 34-35 0.5719845710715958 1107 36-37 0.5341237875506408 1107 38-39 0.5820716187133002 1107 40-41 0.6971422059176646 1107 42-43 0.551140832879085 1107 44-45 0.5077532550389137 1107 46-47 0.5605709864124933 1107 48-49 0.4763600982476177 1107 50-51 0.48580171025776764 1107 52-53 0.4539393746929008 1107 54-55 0.4914656496324241 1107 56-57 0.5523571454138221 1107 58-59 0.5595652961669302 1107 60-61 0.5017747931369243 1107 62-63 0.630265526444866 1107 64-65 0.5686973603537169 1107 66-67 0.7462241465072594 1107 68-69 0.7860643234032949 1107 70-71 0.8560547888934309 1107 72-73 0.8388033594801669 1107 74-75 0.8281973558322484 1107 76-77 0.8685628315964493 1107 78-79 0.9246505684338544 1107 80-81 0.9170113260390522 1107 82-83 0.9913903388283423 1107 84-85 0.9379897304127205 1107 86-87 0.9735082971077595 1107 88-89 1.0666405572942423 1107 90-91 1.2148124713043025 1107 92-93 1.0943230062627158 1107 94-95 1.1849533214372663 1107 96-97 1.3380235003190784 1107 98-99 1.5019877049037973 1107 100-101 1.5482944877249345 1108 1 0.05625216584564896 1108 2 0.10703684890430054 1108 3 0.023502007706238004 1108 4 0.11177969308734248 1108 5 0.0870175362560488 1108 6 0.23050839026929282 1108 7 0.312811630556169 1108 8 0.2494279787270841 1108 9 0.29514484221746073 1108 10-11 0.2777393762104836 1108 12-13 0.35952480908250806 1108 14-15 0.5327135636283558 1108 16-17 0.5536277755454364 1108 18-19 0.4901150455651191 1108 20-21 0.4280994907919151 1108 22-23 0.4075010976725153 1108 24-25 0.4795305992284469 1108 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-1.6277042453130726 2316 100-101 -1.2405349313597789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11181.0 3 2333.0 4 2140.0 5 2386.0 6 2775.0 7 3325.0 8 3981.0 9 5085.0 10 6441.0 11 8314.0 12 11155.0 13 13789.0 14 15806.0 15 19560.0 16 24281.0 17 30935.0 18 27059.0 19 20036.0 20 19674.0 21 20984.0 22 22804.0 23 25585.0 24 28433.0 25 32124.0 26 36775.0 27 43494.0 28 50840.0 29 60716.0 30 73751.0 31 91027.0 32 114023.0 33 146613.0 34 196147.0 35 270428.0 36 381852.0 37 500380.0 38 351724.0 39 33031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44591909013596 19.02644518115049 15.024769458624679 22.50286627008887 2 14.972993770069698 20.013910058155883 41.34343045710819 23.669665714666234 3 18.999079593008457 24.77670512054076 30.00926326007565 26.214952026375137 4 12.339509463600441 16.519690230373865 37.90234776313049 33.23845254289521 5 13.098424845797831 37.856942270245156 34.68262157931288 14.36201130464414 6 31.3833681805397 36.50925240453116 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21.17682084618799 27.022818490306477 30.44222860329934 21.358132060206188 30-31 21.285110576299328 26.652711947917822 30.41954345670187 21.64263401908098 32-33 21.1670770253242 26.64910767784067 30.392328997614733 21.7914862992204 34-35 21.751408618209624 26.633404789200267 30.29903716622998 21.316149426360138 36-37 21.626673796273696 26.47541015411 30.238002369842942 21.659913679773354 38-39 21.31706495146718 26.87200261019328 30.1652654367769 21.645667001562643 40-41 21.596364444620175 26.62782690537871 30.180903837409556 21.59490481259156 42-43 21.194914338140524 26.66941996808757 30.019226913613785 22.11643878015812 44-45 21.404426901427712 26.80910512135357 29.95149183399674 21.834976143221976 46-47 21.823934785103486 27.116103235482775 29.33188253645765 21.72807944295609 48-49 21.641431930521996 27.147773816537317 29.213959467220963 21.996834785719724 50-51 21.786193588113996 27.38486802591248 29.10600321041526 21.722935175558263 52-53 21.892476211003107 27.43578662196799 28.85349586144099 21.818241305587915 54-55 21.71837492740493 27.509220936426953 28.828820505448306 21.94358363071981 56-57 21.80211478157794 27.708301810033248 28.59601641557954 21.89356699280927 58-59 21.887302158565795 27.242849552198656 28.833469926476184 22.036378362759365 60-61 21.71832642841018 27.450053075477282 29.05234871456847 21.779271781544068 62-63 21.616831507012048 27.474281820733097 29.27204633771021 21.63684033454464 64-65 21.591740659089727 27.466986302356865 29.012056357220967 21.92921668133244 66-67 21.550083901312178 27.49829250715004 28.725396538311117 22.226227053226665 68-69 21.643356294502585 27.467266384175677 28.983231978265906 21.906145343055833 70-71 21.858988392984983 27.390338418619258 28.769600982878153 21.981072205517606 72-73 21.629793819594408 27.455231326903363 28.68908442657431 22.225890426927915 74-75 21.49771596698429 27.903964379246478 28.521082848736505 22.077236805032722 76-77 21.694461365580985 27.55994008531948 28.590506586494662 22.15509196260487 78-79 21.684411322237057 27.4920890899839 28.633207603786005 22.190291983993042 80-81 21.757837780714514 27.67819747070803 28.460271204865776 22.103693543711678 82-83 21.74294426446965 27.51462045024798 28.64654951831725 22.095885766965125 84-85 21.68448409030828 27.624688559169 28.403219051690265 22.287608298832453 86-87 21.692911938512115 27.766994523970812 28.398539947229278 22.141553590287792 88-89 21.795013193328682 27.584485613517035 28.30015649469616 22.32034469845813 90-91 21.758616548576917 27.389086474951736 28.42870735605214 22.42358962041921 92-93 21.80225872117136 27.213649647159105 28.52752683646948 22.456564795200055 94-95 21.64755477460444 27.173336107019452 28.58089559639763 22.598213521978476 96-97 21.639099430673504 27.18711844529546 28.582381268997487 22.59140085503355 98-99 21.4490065319749 27.287858618909013 28.46257947882766 22.80055537028843 100-101 21.746325288230615 26.592485926026626 28.983592955458676 22.67759583028408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3425.0 1 1998.0 2 552.0 3 543.0 4 498.5 5 491.5 6 811.5 7 1793.5 8 2919.0 9 3412.0 10 3228.0 11 2942.0 12 3070.5 13 3341.0 14 3545.0 15 3779.5 16 3995.5 17 4309.5 18 4686.0 19 5021.5 20 5514.0 21 6357.0 22 7498.0 23 9096.0 24 11239.0 25 13930.0 26 17758.0 27 22095.0 28 25590.5 29 29512.5 30 34859.5 31 40689.0 32 48238.5 33 54993.5 34 60679.0 35 66496.0 36 73972.0 37 80818.0 38 83631.0 39 85321.0 40 86820.0 41 89083.0 42 90003.0 43 93339.5 44 99454.5 45 102401.0 46 101111.0 47 102322.0 48 105998.5 49 103972.5 50 98055.0 51 92294.0 52 87858.5 53 84110.0 54 82308.0 55 75608.0 56 65577.5 57 58627.5 58 53338.0 59 47216.5 60 38282.0 61 31057.5 62 24736.5 63 17939.0 64 12867.5 65 9682.0 66 7037.0 67 5049.0 68 3810.5 69 2931.0 70 2218.5 71 1490.5 72 1016.0 73 726.5 74 496.5 75 329.0 76 245.0 77 187.0 78 118.0 79 79.0 80 60.5 81 51.0 82 35.5 83 20.0 84 17.5 85 16.0 86 14.0 87 10.0 88 5.5 89 3.5 90 4.0 91 4.5 92 3.5 93 1.0 94 1.0 95 2.0 96 1.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32715022240977176 2 0.29166499138505647 3 0.28900913209838336 4 0.29310358183200436 5 0.3026204109425829 6 0.1979352907262189 7 0.1985254816788129 8 0.19915255956594408 9 0.19826727313705306 10-11 0.20479626055012437 12-13 0.19387772792713504 14-15 0.19675490882103086 16-17 0.20256460101062823 18-19 0.19500277943051736 20-21 0.1885475658865203 22-23 0.1868507668978125 24-25 0.19162762492037033 26-27 0.18666633222512685 28-29 0.19985341132214945 30-31 0.20546022537179265 32-33 0.2076734414440202 34-35 0.20610574672619233 36-37 0.1807644226991867 38-39 0.1724095320265276 40-41 0.17799790260890222 42-43 0.18976483472624545 44-45 0.19391461486167214 46-47 0.19725288243728206 48-49 0.19642292641019674 50-51 0.19985341132214945 52-53 0.22353482329498445 54-55 0.18637123674882985 56-57 0.17172712373759078 58-59 0.17163490640124796 60-61 0.1646632757737311 62-63 0.17268618403555605 64-65 0.1686655081710093 66-67 0.16722691772406137 68-69 0.17794257220709653 70-71 0.171505802130368 72-73 0.16770644787304403 74-75 0.17335014885722433 76-77 0.17726016391815969 78-79 0.1812623963154379 80-81 0.1717640106721279 82-83 0.16929258605814043 84-85 0.16280048557960625 86-87 0.16128812126358408 88-89 0.16619408355702184 90-91 0.1738112355389384 92-93 0.16434973683016554 94-95 0.16969834233804884 96-97 0.16759578706943265 98-99 0.17279684483916743 100-101 0.14902321552998965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2710987.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.514420707755704 #Duplication Level Percentage of deduplicated Percentage of total 1 71.88871877890581 34.876395469813744 2 14.702855453152381 14.266010301191095 3 5.604088331909788 8.156372970530889 4 2.63288697350255 5.109319452338894 5 1.410071150277748 3.4204392506221843 6 0.8427699712377282 2.4531898166694193 7 0.5650181497064117 1.9188069755662185 8 0.38768678998186 1.504672002561543 9 0.28938812171315803 1.2635547376157428 >10 1.472057452030902 13.060846848830904 >50 0.1261655195321566 4.236190544887595 >100 0.07259370829176057 6.415532105985705 >500 0.004483685142831212 1.4520976497929123 >1k 9.879306246851837E-4 0.8163394648237873 >5k 1.5198932687464364E-4 0.5889353541276839 >10k+ 7.599466343732182E-5 0.4612970546416415 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12512 0.4615293249285223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9371 0.3456674635474091 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6603 0.24356442874864395 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3601 0.13282985126819125 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3060 0.11287401968360601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.377386907425229E-5 7 0.0 3.6886934537126144E-5 0.0 0.0 7.377386907425229E-5 8 3.6886934537126144E-5 3.6886934537126144E-5 0.0 0.0 7.377386907425229E-5 9 3.6886934537126144E-5 3.6886934537126144E-5 0.0 3.6886934537126144E-5 7.377386907425229E-5 10-11 3.6886934537126144E-5 3.6886934537126144E-5 0.0 3.6886934537126144E-5 7.377386907425229E-5 12-13 3.6886934537126144E-5 3.6886934537126144E-5 0.0 3.6886934537126144E-5 7.377386907425229E-5 14-15 3.6886934537126144E-5 3.6886934537126144E-5 0.0 3.6886934537126144E-5 7.377386907425229E-5 16-17 7.377386907425229E-5 3.6886934537126144E-5 0.0 3.6886934537126144E-5 7.377386907425229E-5 18-19 1.6599120541706765E-4 3.6886934537126144E-5 0.0 3.6886934537126144E-5 9.221733634281536E-5 20-21 2.58208541759883E-4 3.6886934537126144E-5 0.0 5.533040180568921E-5 1.1066080361137844E-4 22-23 2.58208541759883E-4 3.6886934537126144E-5 0.0 1.4754773814850458E-4 1.291042708799415E-4 24-25 2.9509547629700915E-4 3.6886934537126144E-5 0.0 2.58208541759883E-4 1.4754773814850458E-4 26-27 2.9509547629700915E-4 3.6886934537126144E-5 0.0 3.319824108341353E-4 1.4754773814850458E-4 28-29 4.241997471769507E-4 3.6886934537126144E-5 0.0 0.0011066080361137843 1.4754773814850458E-4 30-31 4.4264321444551375E-4 3.6886934537126144E-5 0.0 0.0026005288848673933 1.6599120541706765E-4 32-33 4.4264321444551375E-4 3.6886934537126144E-5 0.0 0.004426432144455137 1.8443467268563073E-4 34-35 4.6108668171407683E-4 3.6886934537126144E-5 0.0 0.005994126862282999 1.8443467268563073E-4 36-37 5.16417083519766E-4 3.6886934537126144E-5 0.0 0.008447108009001888 1.8443467268563073E-4 38-39 5.533040180568921E-4 3.6886934537126144E-5 0.0 0.013777270049616616 1.8443467268563073E-4 40-41 5.533040180568921E-4 3.6886934537126144E-5 0.0 0.018627901941248703 1.8443467268563073E-4 42-43 5.533040180568921E-4 3.6886934537126144E-5 0.0 0.02340475996380654 1.8443467268563073E-4 44-45 5.533040180568921E-4 3.6886934537126144E-5 0.0 0.026521705932193697 1.8443467268563073E-4 46-47 5.717474853254552E-4 3.6886934537126144E-5 0.0 0.03126167702021441 1.8443467268563073E-4 48-49 5.901909525940183E-4 3.6886934537126144E-5 0.0 0.03843618578768544 2.58208541759883E-4 50-51 5.901909525940183E-4 3.6886934537126144E-5 0.0 0.045776685760573546 2.58208541759883E-4 52-53 6.086344198625814E-4 3.6886934537126144E-5 0.0 0.05920352993208747 2.58208541759883E-4 54-55 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.06975319320970555 2.58208541759883E-4 56-57 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.09653310768365914 2.58208541759883E-4 58-59 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.14061299445552486 2.58208541759883E-4 60-61 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.18157593525900345 2.58208541759883E-4 62-63 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.20564466004447826 2.58208541759883E-4 64-65 6.270778871311445E-4 3.6886934537126144E-5 0.0 0.24625717496985416 2.58208541759883E-4 66-67 7.008517562053968E-4 3.6886934537126144E-5 0.0 0.31890599254072405 2.58208541759883E-4 68-69 7.008517562053968E-4 3.6886934537126144E-5 0.0 0.4199761931724497 2.58208541759883E-4 70-71 7.377386907425229E-4 3.6886934537126144E-5 0.0 0.5137612242330929 2.766520090284461E-4 72-73 8.115125598167752E-4 3.6886934537126144E-5 0.0 0.624016271564563 3.135389435655722E-4 74-75 8.483994943539014E-4 3.6886934537126144E-5 0.0 0.7564956969546515 3.504258781026984E-4 76-77 8.483994943539014E-4 3.6886934537126144E-5 0.0 0.8639842241958371 3.6886934537126145E-4 78-79 8.483994943539014E-4 3.6886934537126144E-5 0.0 1.0049107575949277 3.6886934537126145E-4 80-81 9.590602979652798E-4 3.6886934537126144E-5 0.0 1.1493231063077765 3.873128126398245E-4 82-83 9.959472325024058E-4 3.6886934537126144E-5 0.0 1.261238065693417 4.4264321444551375E-4 84-85 0.001014390699770969 3.6886934537126144E-5 0.0 1.4200732058102825 4.4264321444551375E-4 86-87 0.001032834167039532 3.6886934537126144E-5 0.0 1.6376139022429839 4.4264321444551375E-4 88-89 0.001032834167039532 3.6886934537126144E-5 0.0 1.9311232403548966 4.4264321444551375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9600 0.0 34.486576 1 GTATCAA 14850 0.0 34.370457 1 ATCAACG 18320 0.0 27.668335 3 TATCAAC 18520 0.0 27.42089 2 TCAACGC 18575 0.0 27.315617 4 CAACGCA 18710 0.0 27.09311 5 AACGCAG 19305 0.0 26.353924 6 GTACATG 12205 0.0 25.83972 1 TACATGG 12200 0.0 25.256111 2 ACATGGG 11750 0.0 24.523705 3 ACGCAGA 21545 0.0 23.481657 7 CGCAGAG 21605 0.0 23.327202 8 TAGGTCG 185 2.5171903E-7 23.133759 5 GAGTACT 11945 0.0 23.00439 12-13 GCAGAGT 23110 0.0 21.37642 9 GTACTTT 12960 0.0 21.184801 14-15 AGAGTAC 18080 0.0 21.135529 10-11 AGTACTT 12725 0.0 20.829073 12-13 CATGGGG 5780 0.0 20.567787 4 TATCCCG 120 0.004734651 19.813637 5 >>END_MODULE