FastQCFastQC Report
Thu 26 May 2016
SRR937823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710987
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150720.5559598773435653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120800.4455941692084839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84600.3120634661840872No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39120.1443016879092375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.11331666289805152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114150.033.4340441
GTATCAA169150.031.7508741
TATCAAC208000.025.589152
ATCAACG208250.025.5584323
TCAACGC209350.025.4241354
CAACGCA211350.025.1610645
AACGCAG216950.024.5203786
GGACCGA10050.024.1031026
GTACATG115400.022.9814621
TACATGG116200.022.4526392
GAGTACT137150.021.9230412-13
AGGACCG11500.021.901775
ACGCAGA243100.021.6690277
CGCAGAG242900.021.6282068
ACATGGG113900.021.23713
AGTACTT143200.020.94706512-13
AGAGTAC197450.020.83308610-11
GTACTTT145450.020.57290614-15
GCAGAGT260950.019.5860949
CATGGGG53900.019.132524
Produced by FastQC (version 0.11.5)