FastQCFastQC Report
Thu 26 May 2016
SRR937822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693951
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124110.46069880261370755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96700.3589523343223392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.23641855401230388No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36390.13508040792130221No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28780.10683193569593508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141400.034.7039221
GGTATCA91550.034.0716971
ATCAACG171500.028.159983
TCAACGC172050.028.0725734
CAACGCA172850.027.803585
TATCAAC174300.027.762672
AACGCAG177800.027.0678776
GTACATG121650.024.7032031
TACATGG120750.024.3676912
ACGCAGA200350.023.9036317
CGCAGAG200250.023.8211088
ACATGGG120850.023.4030843
GAGTACT108850.022.47620612-13
GTCTACG1553.877494E-521.4740831
GCAGAGT216500.021.4402269
AGAGTAC170550.021.11357110-11
AGTACTT116200.020.40039612-13
GTACTTT119150.020.31243714-15
CATGGGG55700.019.885844
CATGGGA61050.019.622674
Produced by FastQC (version 0.11.5)