FastQCFastQC Report
Thu 26 May 2016
SRR937822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693951
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.553796264297309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118900.44135917839634053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83590.3102877520786384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39720.1474414345324024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.1116575617002685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122750.030.9304621
GTATCAA179000.029.9710561
ATCAACG215000.024.6823863
TCAACGC217100.024.4655194
TATCAAC217550.024.4149112
CAACGCA217900.024.3320885
AACGCAG223150.023.8394856
GTACATG119050.023.5496121
TACATGG118400.023.3931832
ACATGGG116850.022.768363
GGACCGA9900.022.5495766
GAGTACT134350.022.43291912-13
AGGACCG11800.021.338625
ACGCAGA250200.021.1869647
GTACTTT141400.021.1792914-15
CGCAGAG250950.020.9911488
AGTACTT141100.020.92213212-13
AGAGTAC200700.020.50751510-11
TATTCCG3052.0918378E-1020.2495775
CATGGGG56100.019.9011154
Produced by FastQC (version 0.11.5)