##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937822_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2693951 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.234629731572696 33.0 31.0 34.0 31.0 34.0 2 32.44992726296803 34.0 31.0 34.0 31.0 34.0 3 32.540829807223666 34.0 31.0 34.0 31.0 34.0 4 36.15923600689099 37.0 35.0 37.0 35.0 37.0 5 36.07578497159005 37.0 35.0 37.0 35.0 37.0 6 36.111807898510406 37.0 35.0 37.0 35.0 37.0 7 36.05956752739749 37.0 35.0 37.0 35.0 37.0 8 36.037364079747555 37.0 35.0 37.0 35.0 37.0 9 37.71294429631423 39.0 37.0 39.0 35.0 39.0 10-11 37.6500016147287 39.0 37.0 39.0 35.0 39.0 12-13 37.56212900680079 39.0 37.0 39.0 35.0 39.0 14-15 38.78362060037469 40.0 38.0 41.0 35.0 41.0 16-17 38.754382503616434 40.0 38.0 41.0 35.0 41.0 18-19 38.75626524016212 40.0 38.0 41.0 35.0 41.0 20-21 38.64502193989423 40.0 38.0 41.0 34.0 41.0 22-23 38.49295737004867 40.0 38.0 41.0 34.0 41.0 24-25 38.57087304112065 40.0 38.0 41.0 34.0 41.0 26-27 38.4438731439436 40.0 38.0 41.0 34.0 41.0 28-29 38.30827342442383 40.0 38.0 41.0 34.0 41.0 30-31 38.32654621409223 40.0 38.0 41.0 34.0 41.0 32-33 38.242334585892614 40.0 38.0 41.0 34.0 41.0 34-35 38.114841175656125 40.0 37.5 41.0 34.0 41.0 36-37 37.95218936795807 40.0 37.0 41.0 33.0 41.0 38-39 37.80762085130725 40.0 37.0 41.0 33.0 41.0 40-41 37.63232608907883 40.0 37.0 41.0 33.0 41.0 42-43 37.406468974380005 40.0 37.0 41.0 33.0 41.0 44-45 37.08717493376828 40.0 36.5 41.0 31.5 41.0 46-47 36.722799523822076 40.0 36.0 41.0 31.0 41.0 48-49 36.37686617165643 39.0 36.0 41.0 30.0 41.0 50-51 36.11150462647613 39.0 35.5 41.0 29.5 41.0 52-53 35.875106302972846 39.0 35.0 40.5 29.0 41.0 54-55 35.656699398021715 39.0 35.0 40.0 28.0 41.0 56-57 35.39229017157328 38.5 35.0 40.0 28.0 41.0 58-59 35.127460558859454 38.0 34.5 40.0 27.5 41.0 60-61 34.775644397392526 38.0 34.0 40.0 26.5 41.0 62-63 34.42862657858291 37.5 34.0 40.0 26.0 41.0 64-65 34.045810038861134 37.0 34.0 40.0 26.0 41.0 66-67 33.61019985144496 36.5 33.0 39.0 24.5 40.5 68-69 33.163871020668154 36.0 32.5 39.0 23.5 40.0 70-71 32.74472419876976 35.5 32.0 38.5 22.5 40.0 72-73 32.255045841591034 35.0 31.5 37.5 21.5 40.0 74-75 31.752447984391697 35.0 31.0 37.0 20.0 39.0 76-77 30.52476566945724 33.5 29.5 35.5 19.5 38.5 78-79 31.113060334059526 34.0 31.0 36.0 20.0 38.5 80-81 30.91565715189326 34.0 31.0 36.0 18.5 37.5 82-83 30.555114216999492 34.0 31.0 35.0 17.5 37.0 84-85 30.171476392851986 34.0 30.0 35.0 12.5 36.5 86-87 29.754797321851807 34.0 30.0 35.0 7.0 36.0 88-89 29.38053197701072 34.0 29.5 35.0 4.5 36.0 90-91 28.858372516797818 34.0 29.0 35.0 2.0 35.0 92-93 28.3892001005215 34.0 29.0 35.0 2.0 35.0 94-95 27.923801323780573 33.0 27.5 35.0 2.0 35.0 96-97 27.38082392738398 33.0 27.0 35.0 2.0 35.0 98-99 26.5241656214237 33.0 25.5 34.5 2.0 35.0 100-101 23.890152604854357 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3138894683663409 1101 2 0.2738444042865922 1101 3 0.21510649266625137 1101 4 0.11927781421112371 1101 5 0.08592970157068436 1101 6 0.13890790564855848 1101 7 0.11336722480737649 1101 8 0.1351364019928596 1101 9 0.2688056408386146 1101 10-11 0.2309163015096587 1101 12-13 0.3057563898021627 1101 14-15 0.3209934582380569 1101 16-17 0.32838220863941814 1101 18-19 0.3200384771907494 1101 20-21 0.3234259472375882 1101 22-23 0.33102285742557314 1101 24-25 0.37343639467277967 1101 26-27 0.31088099307372374 1101 28-29 0.2679177628085725 1101 30-31 0.36358126578942773 1101 32-33 0.39510171000434013 1101 34-35 0.37906042613212065 1101 36-37 0.28319112318256145 1101 38-39 0.20978502547451683 1101 40-41 0.24984055538259042 1101 42-43 0.28902960706412273 1101 44-45 0.28317379560544254 1101 46-47 0.3221152675697212 1101 48-49 0.3033071205003708 1101 50-51 0.2737678637631973 1101 52-53 0.3023248644809371 1101 54-55 0.32290294758673355 1101 56-57 0.23153716572379324 1101 58-59 0.17002469542057952 1101 60-61 0.19733338877722417 1101 62-63 0.25272934228040356 1101 64-65 0.2775200277538872 1101 66-67 0.08128384831152147 1101 68-69 0.046077231157518383 1101 70-71 0.3080904088515197 1101 72-73 0.19035887693971176 1101 74-75 0.3321834301898008 1101 76-77 0.12383007758040065 1101 78-79 0.24515058638379017 1101 80-81 0.27716562004744105 1101 82-83 0.23203153798576537 1101 84-85 0.19162283720128315 1101 86-87 0.21276265730739752 1101 88-89 0.40670043159455105 1101 90-91 0.2822231958722199 1101 92-93 0.45576027625870097 1101 94-95 0.441277489729174 1101 96-97 0.6335474756751331 1101 98-99 0.5575407234856691 1101 100-101 0.9259323632779086 1102 1 0.24760956617313212 1102 2 0.19074582583197497 1102 3 0.19898743242954708 1102 4 0.11817609329516188 1102 5 0.056764313272168465 1102 6 0.10952868122289061 1102 7 0.14146110816442103 1102 8 0.14446384088685704 1102 9 0.2690543415517084 1102 10-11 0.15378305371077516 1102 12-13 0.2013462521102909 1102 14-15 0.1470958764457393 1102 16-17 0.18229843136369794 1102 18-19 0.1956276886932784 1102 20-21 0.16509772737685324 1102 22-23 0.31782080087986486 1102 24-25 0.1738157213679017 1102 26-27 0.17761808620400643 1102 28-29 0.13177736544526653 1102 30-31 0.18530236769628772 1102 32-33 0.22843852979627144 1102 34-35 0.15675685320935173 1102 36-37 0.2130447384076959 1102 38-39 0.2746575605482917 1102 40-41 0.23546937305475524 1102 42-43 0.24518971454001814 1102 44-45 0.29864669884924666 1102 46-47 0.24211150219444022 1102 48-49 0.25626317252763187 1102 50-51 0.12843716154394258 1102 52-53 0.260280498133163 1102 54-55 0.17053308546455526 1102 56-57 0.0187493004591488 1102 58-59 0.17637237436887432 1102 60-61 0.0355094130987581 1102 62-63 0.08828819835009938 1102 64-65 0.1240263638113035 1102 66-67 0.20983654961964504 1102 68-69 0.1584678725729347 1102 70-71 -0.050541830198675086 1102 72-73 0.03022350937401086 1102 74-75 -0.02768882596728517 1102 76-77 -0.17346024679970995 1102 78-79 0.002995118222127502 1102 80-81 -0.028102883750474206 1102 82-83 0.010101016132644247 1102 84-85 0.04854903584574899 1102 86-87 0.02566069466363885 1102 88-89 0.059464263537947204 1102 90-91 0.009381081311840234 1102 92-93 0.12508711083321344 1102 94-95 0.3030068661644343 1102 96-97 0.4452020094658913 1102 98-99 0.4212504084027877 1102 100-101 0.800673203189028 1103 1 0.2379125056299003 1103 2 0.24985323425276107 1103 3 0.22800533426079994 1103 4 0.1505477971800886 1103 5 0.1494531197637059 1103 6 0.09850616751276675 1103 7 0.1803010697047185 1103 8 0.1772876721446579 1103 9 0.18915022900215206 1103 10-11 0.20161955428854128 1103 12-13 0.20067261697541738 1103 14-15 0.24186564954273138 1103 16-17 0.30527213585128976 1103 18-19 0.27986044278680566 1103 20-21 0.11693474027153883 1103 22-23 0.27703744881804226 1103 24-25 0.22965082103098666 1103 26-27 0.20898921925086 1103 28-29 0.22233496182681023 1103 30-31 0.3593340605032651 1103 32-33 0.4078433258628209 1103 34-35 0.3790984135582818 1103 36-37 0.36350709415172133 1103 38-39 0.3921943146664617 1103 40-41 0.3084361602373846 1103 42-43 0.46877555559959205 1103 44-45 0.4437023273281113 1103 46-47 0.5720155505760474 1103 48-49 0.45530958546224554 1103 50-51 0.5514897156080139 1103 52-53 0.45121236049753577 1103 54-55 0.5040512652713574 1103 56-57 0.4171099633500006 1103 58-59 0.4089012383959627 1103 60-61 0.36701689020491557 1103 62-63 0.3721122154382783 1103 64-65 0.4431019423623752 1103 66-67 0.3541443648138909 1103 68-69 0.20028824286820424 1103 70-71 0.3361747240542954 1103 72-73 0.29989984110279977 1103 74-75 0.36478930431213286 1103 76-77 0.3320264978986067 1103 78-79 0.4769498608239502 1103 80-81 0.40407422441050755 1103 82-83 0.38307587564333545 1103 84-85 0.30769341783909354 1103 86-87 0.3790040758423068 1103 88-89 0.34809638508949803 1103 90-91 0.48284217987740163 1103 92-93 0.5335870156079743 1103 94-95 0.49141667143780765 1103 96-97 0.5552237341669048 1103 98-99 0.6061920643362946 1103 100-101 0.9166212230431086 1104 1 0.24818822774250293 1104 2 0.21033447080761647 1104 3 0.18453094506284629 1104 4 0.10046741923973457 1104 5 0.12471874190771359 1104 6 0.12710477929434916 1104 7 0.16479257963830918 1104 8 0.17842421153453358 1104 9 0.2496914560897565 1104 10-11 0.26767709833237774 1104 12-13 0.27027218016934995 1104 14-15 0.3142386031859914 1104 16-17 0.2586177797418614 1104 18-19 0.23454064169291655 1104 20-21 0.2222427307292847 1104 22-23 0.27460619187578317 1104 24-25 0.2543184125517044 1104 26-27 0.2557666028200174 1104 28-29 0.17212569797626287 1104 30-31 0.2387448878570737 1104 32-33 0.29558486977542486 1104 34-35 0.3177440335463828 1104 36-37 0.3332505408890327 1104 38-39 0.3296166901978239 1104 40-41 0.19718887269995378 1104 42-43 0.30881056569956655 1104 44-45 0.28198097397918076 1104 46-47 0.17797148565504273 1104 48-49 0.13759761500758572 1104 50-51 0.21163280240212146 1104 52-53 0.21822647233300785 1104 54-55 0.19431498927741586 1104 56-57 0.3013317502242643 1104 58-59 0.27756180914963835 1104 60-61 0.254451451313237 1104 62-63 0.26582469206306314 1104 64-65 0.2777858438091769 1104 66-67 0.22395670692865366 1104 68-69 0.17145529306809237 1104 70-71 0.2821387203611252 1104 72-73 0.2454174234843265 1104 74-75 0.17032155625362577 1104 76-77 0.24737423208430442 1104 78-79 0.391606297854981 1104 80-81 0.27080502158158026 1104 82-83 0.38511813147483664 1104 84-85 0.5085984312666625 1104 86-87 0.42547916336755875 1104 88-89 0.4694553581265666 1104 90-91 0.4204039865297915 1104 92-93 0.5142822496413544 1104 94-95 0.4957038854868365 1104 96-97 0.8144777425187151 1104 98-99 0.9725912732111439 1104 100-101 1.2798943627507455 1105 1 0.18995394947386046 1105 2 0.18112088473731802 1105 3 0.15849144673958193 1105 4 0.11848934396238775 1105 5 0.04553977103908835 1105 6 0.13578324194487124 1105 7 0.09344327966396548 1105 8 0.0862864130848493 1105 9 0.14251938594802027 1105 10-11 0.11964925467447074 1105 12-13 0.1963126473659358 1105 14-15 0.23954801141577065 1105 16-17 0.2869658389711489 1105 18-19 0.24623024521233106 1105 20-21 0.1931950275169143 1105 22-23 0.2301374016345079 1105 24-25 0.20712033550102404 1105 26-27 -0.0014264008575963771 1105 28-29 0.17933338155280154 1105 30-31 0.2049475033453092 1105 32-33 0.22339030122059 1105 34-35 0.1572234490984954 1105 36-37 0.14764054117676295 1105 38-39 0.1734812851588572 1105 40-41 0.09139730025376736 1105 42-43 0.19920104513919057 1105 44-45 0.23908014374281805 1105 46-47 -0.002133405158801338 1105 48-49 -0.0745508527926475 1105 50-51 -0.059556016917092336 1105 52-53 0.05151669569648476 1105 54-55 9.783715613593813E-4 1105 56-57 -0.03655474949476911 1105 58-59 0.07200960713544902 1105 60-61 0.06426792908875001 1105 62-63 -9.908385653716323E-4 1105 64-65 0.10379586489538184 1105 66-67 0.05734279003214482 1105 68-69 -0.08682475712542015 1105 70-71 -0.08622985549489925 1105 72-73 -0.14560414481037753 1105 74-75 -0.15234482425287865 1105 76-77 -0.03125920438021268 1105 78-79 0.03104581344180346 1105 80-81 0.025136472560404854 1105 82-83 0.1444938893246217 1105 84-85 0.14899903983654994 1105 86-87 0.18836899542364094 1105 88-89 0.052780079252524814 1105 90-91 0.071643204878022 1105 92-93 0.11440283033117993 1105 94-95 0.09644483870552278 1105 96-97 0.25149774652338763 1105 98-99 0.5779066297359741 1105 100-101 0.8033757418861462 1106 1 0.1543875661637415 1106 2 0.0725800441044413 1106 3 0.1533215215994872 1106 4 0.08487468349557048 1106 5 0.11526653618293636 1106 6 0.1252169854729459 1106 7 0.12172661337781676 1106 8 0.07838153920007329 1106 9 0.10346685463801464 1106 10-11 0.09348982666402605 1106 12-13 -0.03302001879844596 1106 14-15 0.20883651885990417 1106 16-17 0.2477308191970664 1106 18-19 0.17015253271394926 1106 20-21 0.14744559765463805 1106 22-23 0.2481955150938191 1106 24-25 0.1996045378028839 1106 26-27 0.15211710971944115 1106 28-29 0.29937797152294365 1106 30-31 0.221327547383531 1106 32-33 0.18453516186345809 1106 34-35 0.19096843581854017 1106 36-37 0.06964440086326817 1106 38-39 0.05880195116088771 1106 40-41 0.18557799522564267 1106 42-43 0.01792848379389511 1106 44-45 -0.07708008969020597 1106 46-47 0.13120509683128745 1106 48-49 0.07006974877558747 1106 50-51 0.09238616135147026 1106 52-53 0.013912973756255553 1106 54-55 0.050905217627985166 1106 56-57 -0.0884694672924482 1106 58-59 -0.062147476905018095 1106 60-61 -0.03013393999899705 1106 62-63 -0.08535822034582452 1106 64-65 -0.09004763071474287 1106 66-67 -0.049885750846534904 1106 68-69 0.025424198361982064 1106 70-71 0.06472853396433464 1106 72-73 0.04848066048029409 1106 74-75 -0.07192052509736868 1106 76-77 -0.18480953251064136 1106 78-79 -0.06599492445212363 1106 80-81 0.048663532222558814 1106 82-83 0.007456468249692705 1106 84-85 0.15532239988201368 1106 86-87 0.12506230445477584 1106 88-89 -0.1289790651476963 1106 90-91 0.10596356139484087 1106 92-93 -0.015867614084513093 1106 94-95 0.3885933319945174 1106 96-97 0.2606012414232275 1106 98-99 0.3555824084591208 1106 100-101 0.4665981171597977 1107 1 0.3245770570229354 1107 2 0.40454596541567867 1107 3 0.3084833861005478 1107 4 0.20546651795991977 1107 5 0.17371760600902064 1107 6 0.2183446971205214 1107 7 0.2438620010738859 1107 8 0.18316701774793387 1107 9 0.30682651644688974 1107 10-11 0.2811312031612303 1107 12-13 0.31046461056336483 1107 14-15 0.4356322321390991 1107 16-17 0.38376745656866973 1107 18-19 0.4077229891978007 1107 20-21 0.40539512856278037 1107 22-23 0.45730294269051086 1107 24-25 0.4726257303715755 1107 26-27 0.45233126034798943 1107 28-29 0.3692411376148357 1107 30-31 0.43553611711691076 1107 32-33 0.34123594430125337 1107 34-35 0.47319995953270677 1107 36-37 0.4024400399777406 1107 38-39 0.4435152118128016 1107 40-41 0.4673204953183543 1107 42-43 0.500865962935066 1107 44-45 0.6009569555873568 1107 46-47 0.665446020923703 1107 48-49 0.684473464428045 1107 50-51 0.5543512483096009 1107 52-53 0.5364497779984845 1107 54-55 0.5530513592084318 1107 56-57 0.6296883737725523 1107 58-59 0.6442761148923708 1107 60-61 0.9081169777894118 1107 62-63 0.8794297612613207 1107 64-65 0.8713612208445696 1107 66-67 0.780687264320143 1107 68-69 0.7176707404319203 1107 70-71 0.785924810895402 1107 72-73 0.8253514655383327 1107 74-75 0.7149856936143948 1107 76-77 0.6297763502349376 1107 78-79 0.7237990830385108 1107 80-81 0.7040432555881537 1107 82-83 0.632389256472333 1107 84-85 0.7976723090302293 1107 86-87 0.784326405022135 1107 88-89 0.8395356936315537 1107 90-91 0.7952805970309882 1107 92-93 0.8184321782542696 1107 94-95 1.2403983158636898 1107 96-97 1.3311782429436043 1107 98-99 1.764688908996817 1107 100-101 1.7777144816052335 1108 1 0.14731928873266043 1108 2 0.15915068768997997 1108 3 0.1034416298875982 1108 4 0.08650578370443895 1108 5 0.05380371467613543 1108 6 0.06244822464307731 1108 7 0.08598168779411708 1108 8 0.08694322656265285 1108 9 0.14051983785674338 1108 10-11 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0.1060957949260839 2316 62-63 0.0977146066040433 2316 64-65 0.10495987117008809 2316 66-67 -0.12429015611308358 2316 68-69 -0.04018359161519669 2316 70-71 0.19633605931596065 2316 72-73 0.2281548589894058 2316 74-75 0.27094307291145014 2316 76-77 0.22303711448385855 2316 78-79 0.04822595300626986 2316 80-81 0.049225549468157936 2316 82-83 -0.0330209437123834 2316 84-85 -0.03796253942886452 2316 86-87 0.09228711884241747 2316 88-89 0.20434053761439586 2316 90-91 0.20518472594699944 2316 92-93 0.33282523553327437 2316 94-95 0.4516026113642049 2316 96-97 0.6624682246419731 2316 98-99 0.5211100618295994 2316 100-101 1.0953122366806767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 17.0 5 51.0 6 80.0 7 224.0 8 646.0 9 1213.0 10 2139.0 11 3589.0 12 5578.0 13 8051.0 14 10658.0 15 14864.0 16 20379.0 17 29078.0 18 26645.0 19 16122.0 20 13294.0 21 13253.0 22 14109.0 23 14946.0 24 17104.0 25 20119.0 26 24467.0 27 30276.0 28 38182.0 29 48034.0 30 60149.0 31 76256.0 32 97993.0 33 129230.0 34 178952.0 35 258260.0 36 396740.0 37 597915.0 38 480248.0 39 45075.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62902040690545 19.884099856201544 15.532906381005388 21.953973355887623 2 15.44723716207162 20.964523853626144 40.23799987453373 23.350239109768513 3 19.358035836583518 24.928701375785973 30.27100344438336 25.442259343247148 4 12.911775448932866 17.267545279337654 37.217885746697036 32.60279352503244 5 13.129486319336642 38.27849347665545 34.32115743836526 14.270862765642642 6 31.55484268273625 37.13987373935161 17.03928542130128 14.265998156610864 7 28.27130114838763 33.62637256579648 20.39428334071407 17.708042945101823 8 26.325126180839963 35.52005957049701 19.370582464194783 18.78423178446824 9 26.40070290810783 17.17102501121958 20.456830877770233 35.971441202902355 10-11 24.682724370265085 27.555549451344884 26.83038778359369 20.93133839479634 12-13 25.790288794097087 24.712224887958048 27.78094754101647 21.716538776928395 14-15 22.59528848149057 25.999953228547955 26.6510044169326 24.75375387302887 16-17 21.37577854979545 29.44121849283821 27.322768676935844 21.86023428043049 18-19 21.286949911115684 27.77177461653905 30.218070039135824 20.723205433209436 20-21 22.014580072169093 26.578490106167486 30.6835202273538 20.723409594309622 22-23 21.88949154215947 26.286781662636294 30.657140773085175 21.166586022119063 24-25 21.752701098257003 26.50663031856005 30.73209771300549 21.008570870177458 26-27 21.270219721296222 26.908977590591903 30.746597310885875 21.074205377225997 28-29 21.21490863945385 27.163167205195265 30.64952506844594 20.972399086904947 30-31 21.383258270102164 26.779254708047773 30.561079247543848 21.276407774306215 32-33 21.319578566944983 26.81073263767604 30.486597566176965 21.383091229202016 34-35 21.818102853392656 26.769343614638867 30.504897824793396 20.907655707175074 36-37 21.82387504449784 26.55829671734935 30.38332916968423 21.23449906846858 38-39 21.438766241503636 27.032101967856086 30.31546277636494 21.213669014275336 40-41 21.787484597354094 26.78084654185427 30.25780303284803 21.173865827943605 42-43 21.409758047106035 26.894912182253865 30.054186622432788 21.64114314820731 44-45 21.577772123132924 27.055415457707927 29.964229112598765 21.402583306560384 46-47 22.146226861587312 27.349847862860166 29.25864278897426 21.24528248657826 48-49 21.936367810698858 27.49348447688915 29.062945094398522 21.50720261801347 50-51 22.092254090738844 27.78365308054972 28.919456961169672 21.204635867541764 52-53 22.262332682925198 27.789533545450645 28.563601507749127 21.384532263875027 54-55 22.024212764077745 28.01424376315679 28.442183989241084 21.519359483524383 56-57 22.15029152349096 28.143997422373307 28.287244274301948 21.418466779833782 58-59 22.177500630115397 27.617280344000317 28.458164235355433 21.747054790528857 60-61 21.99589005145231 27.951083742800076 28.71867379918937 21.33435240655825 62-63 21.859510436529842 27.90353276655737 28.986978605030306 21.24997819188248 64-65 21.911497276676524 27.895997365950603 28.763013878129186 21.429491479243683 66-67 21.89258453475954 27.95850778280674 28.421823559522796 21.727084122910924 68-69 21.877278254814126 27.91100644852833 28.666199199093672 21.545516097563873 70-71 22.179282399716996 27.901472595455523 28.333347562743345 21.585897442084136 72-73 22.00513298126061 27.92203718627399 28.295317917809193 21.777511914656206 74-75 21.804405499580355 28.46907757416523 28.019403470961425 21.707113455292987 76-77 22.047542809798692 28.000787690644707 28.253854654371963 21.697814845184638 78-79 22.007675714962893 28.055818387194126 28.152423707780876 21.784082190062108 80-81 22.101016407700445 28.240313428411866 28.039808481862217 21.61886168202547 82-83 22.050590550998418 28.156591833533945 28.16997400591897 21.622843609548674 84-85 21.907626541704435 28.335419501739835 27.92152665952469 21.835427297031035 86-87 21.945350899106923 28.434036105333764 27.888183563843587 21.732429431715723 88-89 22.038745322390792 28.28549962490038 27.789536632255 21.886218420453822 90-91 22.04037992846178 28.12344890697247 27.884777802733453 21.9513933618323 92-93 21.992083161879794 28.043896560294012 27.964772724530825 21.99924755329537 94-95 21.894941667461655 27.99655598784091 28.005724677249137 22.102777667448294 96-97 21.921297009485325 28.07682842041299 27.951269343800238 22.050605226301442 98-99 21.63891238325281 28.24244030560058 27.682306210474483 22.436341100672127 100-101 21.600710913890797 28.267700588226667 27.994072252360326 22.137516245522214 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 771.0 1 547.0 2 388.5 3 488.0 4 449.0 5 448.0 6 811.0 7 1820.5 8 2985.0 9 3525.5 10 3478.5 11 3353.5 12 3553.5 13 3904.5 14 4221.0 15 4473.0 16 4741.0 17 4991.0 18 5257.5 19 5635.5 20 6218.5 21 6995.5 22 8116.0 23 9634.0 24 11604.5 25 14283.5 26 17912.5 27 21956.5 28 25672.0 29 30053.0 30 35120.5 31 40736.0 32 47760.5 33 54199.0 34 60031.0 35 65487.0 36 73039.0 37 79789.0 38 82711.5 39 85004.5 40 86517.0 41 88693.5 42 89413.0 43 92879.5 44 99320.0 45 101443.0 46 99312.0 47 101051.5 48 105663.5 49 103006.5 50 97128.0 51 91895.5 52 86763.5 53 83764.5 54 83346.0 55 76647.5 56 65159.0 57 57335.5 58 52144.0 59 46392.0 60 37382.0 61 29909.0 62 24159.0 63 17215.5 64 11802.5 65 8710.0 66 6365.0 67 4968.5 68 3884.5 69 2929.5 70 2264.0 71 1454.5 72 973.0 73 728.0 74 498.5 75 362.0 76 251.0 77 155.0 78 102.0 79 59.5 80 34.0 81 27.0 82 20.5 83 11.5 84 8.5 85 6.5 86 4.5 87 2.5 88 1.5 89 1.0 90 1.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022197879619933695 2 0.0 3 0.0 4 2.598414002333376E-4 5 0.023163004820800377 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0034892988031333906 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011321661010166852 24-25 1.8560100016666968E-5 26-27 5.75363100516676E-4 28-29 0.0013548873012166887 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4848080013333574E-4 40-41 5.382429004833422E-4 42-43 0.014458317912983569 44-45 1.8560100016666968E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.5680300050000913E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.1136060010000183E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.280050008333484E-5 82-83 0.002542733702283375 84-85 6.31043400566677E-4 86-87 0.0 88-89 0.0 90-91 0.010616377209533507 92-93 0.0026912145024167106 94-95 0.0 96-97 0.0 98-99 0.0015776085014166923 100-101 0.019172583317216982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2693951.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.359755088887916 #Duplication Level Percentage of deduplicated Percentage of total 1 70.33067703273612 32.60512962473321 2 15.339090713719195 14.222329775485134 3 5.740362409185973 7.98365386233961 4 2.8378916033941577 5.262558388086584 5 1.591781971202207 3.6897311169920775 6 0.9526738061394674 2.6499434599334633 7 0.634380388976025 2.058680362628538 8 0.44100768251490563 1.6356006522967248 9 0.31855656502053215 1.3291383902678342 >10 1.5915472097064096 13.461827068967875 >50 0.13777031155605157 4.3995423690287 >100 0.07662810892645504 6.549316592908211 >500 0.006427113197841323 2.026496326946851 >1k 9.640669796710089E-4 0.8162225143330167 >5k 8.033891497258407E-5 0.3113303215747062 >10k+ 1.6067782994516814E-4 0.998499173477246 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14919 0.553796264297309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11890 0.44135917839634053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8359 0.3102877520786384 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3972 0.1474414345324024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3008 0.1116575617002685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.712020003333394E-5 0.0 0.0 0.0 0.0 8 3.712020003333394E-5 0.0 0.0 3.712020003333394E-5 0.0 9 3.712020003333394E-5 0.0 0.0 3.712020003333394E-5 0.0 10-11 3.712020003333394E-5 0.0 0.0 3.712020003333394E-5 0.0 12-13 1.1136060010000181E-4 0.0 0.0 3.712020003333394E-5 3.712020003333394E-5 14-15 1.4848080013333577E-4 0.0 0.0 3.712020003333394E-5 5.5680300050000913E-5 16-17 1.4848080013333577E-4 0.0 0.0 7.424040006666788E-5 7.424040006666788E-5 18-19 1.6704090015000274E-4 0.0 0.0 7.424040006666788E-5 1.1136060010000181E-4 20-21 1.856010001666697E-4 0.0 0.0 7.424040006666788E-5 1.1136060010000181E-4 22-23 1.856010001666697E-4 0.0 0.0 1.670409001500027E-4 1.1136060010000181E-4 24-25 2.2272120020000363E-4 0.0 0.0 3.897621003500064E-4 1.4848080013333577E-4 26-27 2.2272120020000363E-4 0.0 0.0 6.49603500583344E-4 1.4848080013333577E-4 28-29 2.9696160026667154E-4 0.0 0.0 0.0015219282013666916 1.4848080013333577E-4 30-31 3.340818003000055E-4 0.0 0.0 0.004138902303716734 1.6704090015000274E-4 32-33 3.712020003333394E-4 0.0 0.0 0.006588835505916774 2.598414002333376E-4 34-35 4.640025004166742E-4 0.0 0.0 0.009075888908150148 2.598414002333376E-4 36-37 4.825626004333412E-4 0.0 0.0 0.01256518771128354 2.598414002333376E-4 38-39 5.196828004666752E-4 0.0 0.0 0.01733513341556695 2.598414002333376E-4 40-41 5.196828004666752E-4 0.0 0.0 0.023682687621267053 2.598414002333376E-4 42-43 5.196828004666752E-4 0.0 0.0 0.02995600142690049 2.598414002333376E-4 44-45 5.568030005000091E-4 0.0 0.0 0.03324113912985054 2.598414002333376E-4 46-47 5.939232005333431E-4 0.0 0.0 0.03806676513418396 2.598414002333376E-4 48-49 6.31043400566677E-4 0.0 0.0 0.04521240364060074 2.598414002333376E-4 50-51 6.31043400566677E-4 0.0 0.0 0.05308188604766753 2.598414002333376E-4 52-53 6.31043400566677E-4 0.0 0.0 0.06839396856141777 2.598414002333376E-4 54-55 6.31043400566677E-4 0.0 0.0 0.07949290837138463 2.598414002333376E-4 56-57 6.31043400566677E-4 0.0 0.0 0.11106363849973515 2.7840150025000457E-4 58-59 6.31043400566677E-4 0.0 0.0 0.16078614644438594 2.9696160026667154E-4 60-61 6.68163600600011E-4 0.0 0.0 0.2068894348857867 2.9696160026667154E-4 62-63 6.867237006166779E-4 0.0 0.0 0.2324652527087538 2.9696160026667154E-4 64-65 7.238439006500119E-4 0.0 0.0 0.2753205236472378 2.9696160026667154E-4 66-67 7.424040006666788E-4 0.0 0.0 0.3553887951191391 2.9696160026667154E-4 68-69 7.424040006666788E-4 0.0 0.0 0.4654687483179909 2.9696160026667154E-4 70-71 8.352045007500136E-4 0.0 0.0 0.5649137642072926 3.155217002833385E-4 72-73 8.908848008000145E-4 0.0 0.0 0.6827518392131111 3.712020003333394E-4 74-75 9.280050008333484E-4 0.0 0.0 0.8256460492414301 3.712020003333394E-4 76-77 9.280050008333484E-4 0.0 0.0 0.9381202553424319 3.712020003333394E-4 78-79 9.280050008333484E-4 0.0 0.0 1.0847264853740843 3.712020003333394E-4 80-81 0.0010950459009833513 0.0 0.0 1.23521177630922 3.712020003333394E-4 82-83 0.0011878464010666862 0.0 0.0 1.3512495216134222 3.712020003333394E-4 84-85 0.0012064065010833531 0.0 0.0 1.5155620870609747 3.712020003333394E-4 86-87 0.00122496660110002 0.0 0.0 1.7371696812599784 3.712020003333394E-4 88-89 0.00122496660110002 0.0 0.0 2.0471047914383003 3.712020003333394E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12275 0.0 30.930462 1 GTATCAA 17900 0.0 29.971056 1 ATCAACG 21500 0.0 24.682386 3 TCAACGC 21710 0.0 24.465519 4 TATCAAC 21755 0.0 24.414911 2 CAACGCA 21790 0.0 24.332088 5 AACGCAG 22315 0.0 23.839485 6 GTACATG 11905 0.0 23.549612 1 TACATGG 11840 0.0 23.393183 2 ACATGGG 11685 0.0 22.76836 3 GGACCGA 990 0.0 22.549576 6 GAGTACT 13435 0.0 22.432919 12-13 AGGACCG 1180 0.0 21.33862 5 ACGCAGA 25020 0.0 21.186964 7 GTACTTT 14140 0.0 21.17929 14-15 CGCAGAG 25095 0.0 20.991148 8 AGTACTT 14110 0.0 20.922132 12-13 AGAGTAC 20070 0.0 20.507515 10-11 TATTCCG 305 2.0918378E-10 20.249577 5 CATGGGG 5610 0.0 19.901115 4 >>END_MODULE