FastQCFastQC Report
Thu 26 May 2016
SRR937821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553925
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123850.4849398474896483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92210.3610521060720264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63170.24734477324118756No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35900.14056794933288957No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28050.10983094648433295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97700.032.3669851
GTATCAA146050.031.4847371
ATCAACG174150.026.2267653
TACATGG112200.026.01342
TATCAAC176550.025.9286672
TCAACGC176050.025.8606974
GTACATG114450.025.8018551
CAACGCA177800.025.6066655
ACATGGG110150.025.1552643
AACGCAG182200.025.031116
GAGTACT107800.022.40452812-13
ACGCAGA205300.022.0762487
CATGGGA59300.022.0388414
CGCAGAG205050.022.0104878
GTACTTT114850.020.82327714-15
AGTACTT114850.020.38822712-13
GCAGAGT219000.020.2601669
AGAGTAC170450.020.23133310-11
CATGGGG52100.020.0676144
GTATAGG6900.019.9859161
Produced by FastQC (version 0.11.5)