FastQCFastQC Report
Thu 26 May 2016
SRR937821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553925
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142490.5579255459733548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114260.44738980197147526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76600.29993049913368636No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37770.1478900124318451No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.11492115077772448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167250.031.146891
GGTATCA113550.030.765961
ATCAACG202700.025.5913093
TCAACGC204300.025.3676364
TATCAAC205250.025.2965092
CAACGCA206150.025.1399845
AACGCAG210800.024.6501356
GAGTACT127400.023.34011712-13
GTACATG115950.023.3244691
TACATGG115600.023.176342
ACATGGG113300.022.7244283
GTACTTT134450.022.02663814-15
ACGCAGA235350.021.9993767
GCGGTCG2405.9353624E-921.7710579
CGCAGAG236250.021.754728
AGTACTT134650.021.60716612-13
GGACCGA9750.021.4348586
CGAGCGG2257.163908E-821.1100886
AGAGTAC193050.020.61922310-11
GCAGAGT252700.020.0002069
Produced by FastQC (version 0.11.5)