##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937821_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2553925 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31672895641023 33.0 31.0 34.0 31.0 34.0 2 32.530748945250934 34.0 31.0 34.0 31.0 34.0 3 32.59875094217724 34.0 31.0 34.0 31.0 34.0 4 36.178141879655826 37.0 35.0 37.0 35.0 37.0 5 36.10940376087787 37.0 35.0 37.0 35.0 37.0 6 36.15603159842204 37.0 36.0 37.0 35.0 37.0 7 36.11429348943293 37.0 35.0 37.0 35.0 37.0 8 36.07976624215668 37.0 35.0 37.0 35.0 37.0 9 37.7854917431013 39.0 38.0 39.0 35.0 39.0 10-11 37.71187035641219 39.0 37.0 39.0 35.0 39.0 12-13 37.61515843260863 39.0 37.0 39.0 35.0 39.0 14-15 38.86319508207954 40.0 38.0 41.0 35.0 41.0 16-17 38.83168084419081 40.0 38.0 41.0 35.0 41.0 18-19 38.822709163346616 40.0 38.0 41.0 35.0 41.0 20-21 38.71874408997915 40.0 38.0 41.0 34.5 41.0 22-23 38.669844650880506 40.0 38.0 41.0 34.0 41.0 24-25 38.689138091369166 40.0 38.0 41.0 34.0 41.0 26-27 38.51572403261646 40.0 38.0 41.0 34.0 41.0 28-29 38.48374110437855 40.0 38.0 41.0 34.0 41.0 30-31 38.47615630842722 40.0 38.0 41.0 34.0 41.0 32-33 38.34298423015555 40.0 38.0 41.0 34.0 41.0 34-35 38.200800924067856 40.0 38.0 41.0 34.0 41.0 36-37 38.032594927415644 40.0 37.5 41.0 33.5 41.0 38-39 37.8869177834118 40.0 37.0 41.0 33.0 41.0 40-41 37.71716710553364 40.0 37.0 41.0 33.0 41.0 42-43 37.48487602415889 40.0 37.0 41.0 33.0 41.0 44-45 37.175024716857386 40.0 37.0 41.0 32.0 41.0 46-47 36.81840441673111 40.0 36.0 41.0 31.0 41.0 48-49 36.45903462317805 39.5 36.0 41.0 30.5 41.0 50-51 36.20627054435819 39.0 36.0 41.0 30.0 41.0 52-53 35.951099973570095 39.0 35.0 40.5 29.0 41.0 54-55 35.73775247902738 39.0 35.0 40.0 28.5 41.0 56-57 35.46544123261255 38.5 35.0 40.0 28.0 41.0 58-59 35.21443131650303 38.0 35.0 40.0 28.0 41.0 60-61 34.88199183609542 38.0 34.0 40.0 27.0 41.0 62-63 34.484731344890704 37.5 34.0 40.0 26.0 41.0 64-65 34.10132247423084 37.0 34.0 40.0 26.0 41.0 66-67 33.68527188543125 36.5 33.0 39.0 25.0 40.5 68-69 33.22404005599225 36.0 33.0 39.0 23.5 40.0 70-71 32.80048768072672 35.5 32.0 38.5 23.0 40.0 72-73 32.332382509274936 35.0 32.0 38.0 22.0 40.0 74-75 31.8541047603199 35.0 31.0 37.0 20.5 39.0 76-77 30.59118983525358 34.0 29.5 35.5 20.0 38.5 78-79 31.183283769100502 34.0 31.0 36.0 20.0 38.5 80-81 31.016590933563045 34.0 31.0 36.0 19.0 37.5 82-83 30.634054249831145 34.0 31.0 35.0 18.0 37.0 84-85 30.233377839991384 34.0 30.5 35.0 14.5 36.5 86-87 29.914846951261296 34.0 30.0 35.0 8.5 36.0 88-89 29.504684945720804 34.0 30.0 35.0 4.5 36.0 90-91 29.01237272042053 34.0 29.0 35.0 2.0 35.0 92-93 28.56793210450581 34.0 29.0 35.0 2.0 35.0 94-95 28.093002339536206 33.5 29.0 35.0 2.0 35.0 96-97 27.590625997239542 33.0 27.0 35.0 2.0 35.0 98-99 26.79570053936588 33.0 26.0 35.0 2.0 35.0 100-101 24.360433646250378 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.225910970717905 1101 2 0.21753938641116832 1101 3 0.22792580814994778 1101 4 0.11171275507901157 1101 5 0.11125986682481681 1101 6 0.13701468153174545 1101 7 0.12431583161168902 1101 8 0.1553487468563688 1101 9 0.19483874958817893 1101 10-11 0.2529225378254054 1101 12-13 0.25540462947049747 1101 14-15 0.2787866882286565 1101 16-17 0.30157572088453577 1101 18-19 0.2238458365472482 1101 20-21 0.2784448624118241 1101 22-23 0.2639815572798696 1101 24-25 0.2048320873836218 1101 26-27 0.178844710995115 1101 28-29 0.16054080368407142 1101 30-31 0.19161673692001102 1101 32-33 0.24195248082577336 1101 34-35 0.248528113949547 1101 36-37 0.09802878737585985 1101 38-39 0.2376704716499063 1101 40-41 0.09302412003106042 1101 42-43 0.07874952736273144 1101 44-45 0.14569377824548013 1101 46-47 0.17670099998811395 1101 48-49 0.10622902729715378 1101 50-51 0.042334506258399074 1101 52-53 0.004107020719452237 1101 54-55 0.10405362330881474 1101 56-57 0.10306962560660793 1101 58-59 0.10629358358666252 1101 60-61 0.01098670687179748 1101 62-63 0.05015929596383728 1101 64-65 0.260713197076619 1101 66-67 0.16365298231016823 1101 68-69 0.14921002929587246 1101 70-71 0.15782099138993289 1101 72-73 0.2375820025858033 1101 74-75 0.23200409919263976 1101 76-77 0.20391760809966897 1101 78-79 0.24770320690130632 1101 80-81 0.21227625782546866 1101 82-83 0.22432603928041672 1101 84-85 0.3248765508473852 1101 86-87 0.4076894265034561 1101 88-89 0.608287840388261 1101 90-91 0.6153174353270394 1101 92-93 0.7725843213890968 1101 94-95 0.7129722315932163 1101 96-97 0.7795104231989818 1101 98-99 0.806798151855407 1101 100-101 1.2746134162321 1102 1 0.138518263365917 1102 2 0.14009794622072747 1102 3 0.16968541755303335 1102 4 0.08362343907450764 1102 5 0.026215018487974362 1102 6 0.08474688674355946 1102 7 0.05820428755920659 1102 8 0.12880525101240892 1102 9 0.18305596086317166 1102 10-11 0.1741503515752285 1102 12-13 0.13766911634719037 1102 14-15 0.16583946677455685 1102 16-17 0.10400960953999316 1102 18-19 0.1570084672825729 1102 20-21 0.2207345756051211 1102 22-23 0.14961101612709626 1102 24-25 0.1258877221455066 1102 26-27 0.15967059554221663 1102 28-29 0.24400711186730462 1102 30-31 0.19483905770123755 1102 32-33 0.2322454165393566 1102 34-35 0.21226338845632142 1102 36-37 0.2772661162592769 1102 38-39 0.22229786075806146 1102 40-41 0.3143110292805673 1102 42-43 0.23773776931351165 1102 44-45 0.38743048365342503 1102 46-47 0.23948448181835147 1102 48-49 0.24628196984204465 1102 50-51 0.31084155282812986 1102 52-53 0.4197183678534273 1102 54-55 0.4364837651260416 1102 56-57 0.43775245914204675 1102 58-59 0.3653686931288078 1102 60-61 0.3705275575836353 1102 62-63 0.3024849093739306 1102 64-65 0.3787914720785892 1102 66-67 0.3985440267283522 1102 68-69 0.35493459159613394 1102 70-71 0.36201190377098413 1102 72-73 0.2822144672831257 1102 74-75 0.36061601733041115 1102 76-77 0.326544959699536 1102 78-79 0.1581745224405502 1102 80-81 0.30959449527126637 1102 82-83 0.5107412966929914 1102 84-85 0.3373388779651165 1102 86-87 0.35639066224182514 1102 88-89 0.48701597435508504 1102 90-91 0.48699680493247754 1102 92-93 0.7034828882134967 1102 94-95 0.7777989173811264 1102 96-97 0.8869812355685802 1102 98-99 0.7624049022293917 1102 100-101 1.0544643247904943 1103 1 0.21900289114253724 1103 2 0.22824114463981715 1103 3 0.13523215579439807 1103 4 0.12110196854538202 1103 5 0.15878809349391787 1103 6 0.12599746071854412 1103 7 0.17625295785224182 1103 8 0.12395705129181778 1103 9 0.17673105947823586 1103 10-11 0.19576774309030043 1103 12-13 0.23304701089066526 1103 14-15 0.30382938932675785 1103 16-17 0.2500681196826875 1103 18-19 0.34349487133506074 1103 20-21 0.27934854562347056 1103 22-23 0.34169810684655033 1103 24-25 0.1894012832750036 1103 26-27 0.23943859892819574 1103 28-29 0.09177216435685409 1103 30-31 0.13150306844969606 1103 32-33 0.23447780840749033 1103 34-35 0.1961563656765577 1103 36-37 0.1631603772893797 1103 38-39 0.16594977753855034 1103 40-41 0.24073554918539486 1103 42-43 0.23176327643442818 1103 44-45 0.20058406132287843 1103 46-47 0.22727839983529208 1103 48-49 0.08733578024904176 1103 50-51 0.16793421234094552 1103 52-53 0.12702315960618904 1103 54-55 0.19552833650304535 1103 56-57 0.1711710409040066 1103 58-59 0.1351799001008871 1103 60-61 0.1983271244753766 1103 62-63 0.10952632373184912 1103 64-65 0.24790059466321424 1103 66-67 0.370222635277905 1103 68-69 0.37855993805279553 1103 70-71 0.2903535732335385 1103 72-73 0.3262435885140249 1103 74-75 0.3425733218087963 1103 76-77 0.3404943100719713 1103 78-79 0.42316914444991127 1103 80-81 0.3024138367066094 1103 82-83 0.4134860147652546 1103 84-85 0.45042549362757 1103 86-87 0.6177973574119129 1103 88-89 0.6107965255966903 1103 90-91 0.5801184920853686 1103 92-93 0.7382697065585795 1103 94-95 0.6324305699374442 1103 96-97 0.603248114820115 1103 98-99 0.721667267255615 1103 100-101 1.2018576880032583 1104 1 0.1874149076219851 1104 2 0.15540318913267015 1104 3 0.1546854025065869 1104 4 0.1083688209984004 1104 5 0.11366151225809773 1104 6 0.08108415235757604 1104 7 0.09825534229344157 1104 8 0.11731234713038674 1104 9 0.22318224781777474 1104 10-11 0.17927753486799958 1104 12-13 0.12639825498985147 1104 14-15 0.22077704337707615 1104 16-17 0.14443059184924323 1104 18-19 0.24781913099841546 1104 20-21 0.2580145052393519 1104 22-23 0.27247554955191333 1104 24-25 0.18257477029541747 1104 26-27 0.1733349162533031 1104 28-29 0.25797859498669595 1104 30-31 0.29138069701561875 1104 32-33 0.21267945056410298 1104 34-35 0.17338165737582756 1104 36-37 0.058892129583547614 1104 38-39 0.15366135662308267 1104 40-41 0.3072742372856183 1104 42-43 0.2549910968346225 1104 44-45 0.06559435318613538 1104 46-47 0.0012377966574490529 1104 48-49 -0.03941346175660243 1104 50-51 -0.08465122036478334 1104 52-53 0.00866198280890984 1104 54-55 0.02654060199378705 1104 56-57 0.04523006895851722 1104 58-59 0.02082489916363528 1104 60-61 -0.07123754506965696 1104 62-63 -0.017520713697166457 1104 64-65 0.09685872624441316 1104 66-67 0.28683234754949893 1104 68-69 0.26158666751103254 1104 70-71 0.3500323548977704 1104 72-73 0.3263682574179043 1104 74-75 0.277874761571379 1104 76-77 0.2833906833496691 1104 78-79 0.25815448917500916 1104 80-81 0.3157749296492085 1104 82-83 0.2996910591956201 1104 84-85 0.3371858341339653 1104 86-87 0.3525563983933999 1104 88-89 0.32056791102589344 1104 90-91 0.35813088981419483 1104 92-93 0.41588193194749223 1104 94-95 0.4806797955431925 1104 96-97 0.6746574240549528 1104 98-99 1.0329808070258117 1104 100-101 1.591968876339827 1105 1 0.16518902918772937 1105 2 0.17974316824429337 1105 3 0.16543066856476685 1105 4 0.11729562789732029 1105 5 0.057030252220840794 1105 6 0.058588215229924856 1105 7 0.13661023235444958 1105 8 0.13858350652481022 1105 9 0.11881295718473694 1105 10-11 0.26626025814690024 1105 12-13 0.20461678219942314 1105 14-15 0.15908246265079384 1105 16-17 0.26486333812070484 1105 18-19 0.37591753085668955 1105 20-21 0.336898585623139 1105 22-23 0.21696042018916017 1105 24-25 0.19973740798697293 1105 26-27 0.22216733212258788 1105 28-29 0.1978532807176805 1105 30-31 0.26045082209644477 1105 32-33 0.13309440078312207 1105 34-35 0.2775884970719318 1105 36-37 0.22712318598151882 1105 38-39 0.3047352916260806 1105 40-41 0.32610735348472275 1105 42-43 0.34657649006501856 1105 44-45 0.4386303117649817 1105 46-47 0.39480525121116017 1105 48-49 0.3050537567315885 1105 50-51 0.2591438094040299 1105 52-53 0.273780792644736 1105 54-55 0.3253244904424335 1105 56-57 0.24815783137594138 1105 58-59 0.2985783466091618 1105 60-61 0.3484315059162739 1105 62-63 0.31396561963808267 1105 64-65 0.3503658117275066 1105 66-67 0.3172534440421586 1105 68-69 0.32594769808954993 1105 70-71 0.2722353047253705 1105 72-73 0.2359192212828063 1105 74-75 0.28048788722334095 1105 76-77 0.26887530839179874 1105 78-79 0.38157802754829007 1105 80-81 0.3869568209746497 1105 82-83 0.3511267673186147 1105 84-85 0.3276483971876374 1105 86-87 0.517855663079061 1105 88-89 0.6254083733331193 1105 90-91 0.6961111833643194 1105 92-93 0.7143519511154217 1105 94-95 0.9685216380438106 1105 96-97 1.0763437479245574 1105 98-99 1.369735923111076 1105 100-101 1.5670441697908188 1106 1 -0.13167791912641746 1106 2 -0.007011082707265359 1106 3 -0.03313232664036292 1106 4 0.008363534101803793 1106 5 0.01276472848586252 1106 6 0.04721115137440535 1106 7 0.00726575004251373 1106 8 0.08955646335609657 1106 9 -0.18820863766949714 1106 10-11 -0.1355893315366501 1106 12-13 -0.1625654857918306 1106 14-15 -0.056303710168982946 1106 16-17 0.03706948565586288 1106 18-19 0.05165893770818997 1106 20-21 0.07574421806709353 1106 22-23 0.09411425144580221 1106 24-25 7.135304745133908E-4 1106 26-27 0.03654907776363103 1106 28-29 0.1104551826845892 1106 30-31 0.07001518378164207 1106 32-33 0.036874617564549794 1106 34-35 -0.17395055358331035 1106 36-37 0.04983221578743979 1106 38-39 0.08240668228702219 1106 40-41 0.07099676543106881 1106 42-43 -0.1223162818783905 1106 44-45 -0.12104958790910558 1106 46-47 0.20789102458267905 1106 48-49 0.2248924843206126 1106 50-51 0.19271877654553293 1106 52-53 0.33477215429676477 1106 54-55 0.2670322316400444 1106 56-57 0.11391267618429879 1106 58-59 0.16882647650196247 1106 60-61 0.21584239523206605 1106 62-63 0.23008292362301574 1106 64-65 -0.12958034841610555 1106 66-67 0.015966850240879182 1106 68-69 0.12312672593851914 1106 70-71 0.191633295092295 1106 72-73 0.24216521342655994 1106 74-75 0.08990505435712848 1106 76-77 -0.05407952531136928 1106 78-79 0.1828955161231498 1106 80-81 0.1246541835597732 1106 82-83 0.04619196629449718 1106 84-85 0.08166624133171041 1106 86-87 0.3462056553257433 1106 88-89 0.14275413815905225 1106 90-91 0.14226100050111867 1106 92-93 0.1346796625592539 1106 94-95 0.076384264813953 1106 96-97 -0.23111172805132796 1106 98-99 -0.4886856874529357 1106 100-101 -0.04944994886001197 1107 1 0.13657473567981526 1107 2 0.1642312569876978 1107 3 0.055310646742618985 1107 4 0.10007431493132657 1107 5 0.15508444389205067 1107 6 0.14200338162381598 1107 7 0.15137655350375212 1107 8 0.08994739086742243 1107 9 0.15563783443514012 1107 10-11 0.14536555001814833 1107 12-13 0.11589610571278541 1107 14-15 0.1245352722525368 1107 16-17 0.09452327629128376 1107 18-19 0.21795244629586108 1107 20-21 0.15614753997848396 1107 22-23 0.14423810704131768 1107 24-25 0.038994545648364465 1107 26-27 0.12540428429570483 1107 28-29 0.09524826162329703 1107 30-31 0.18268213688094903 1107 32-33 0.18629043642596343 1107 34-35 0.06614550743744729 1107 36-37 -0.021781160263252275 1107 38-39 0.14529721197523315 1107 40-41 0.11479620600336915 1107 42-43 0.11603457335288425 1107 44-45 0.10729734213413877 1107 46-47 0.17352865546648388 1107 48-49 0.2354592850094619 1107 50-51 0.35128328930946395 1107 52-53 0.21456548076985626 1107 54-55 0.344914415883423 1107 56-57 0.3735624503975785 1107 58-59 0.38200715184647294 1107 60-61 0.23491995836217683 1107 62-63 0.21482941305507097 1107 64-65 0.16393646093644776 1107 66-67 0.3242365318763021 1107 68-69 0.23410372645834343 1107 70-71 0.17913201321783134 1107 72-73 0.2619012180094771 1107 74-75 0.36147988576853507 1107 76-77 0.11627938185970166 1107 78-79 0.2837238722473252 1107 80-81 0.327586935540932 1107 82-83 0.3434526447508759 1107 84-85 0.4958971696917267 1107 86-87 0.6679693201076127 1107 88-89 0.655832086735554 1107 90-91 0.7491071826369158 1107 92-93 0.6539045970510493 1107 94-95 0.762320510566088 1107 96-97 0.7020802454695705 1107 98-99 0.5355206112361905 1107 100-101 1.0630889051972545 1108 1 0.16637606018313278 1108 2 0.07970360656600661 1108 3 0.06171241274703476 1108 4 0.08909339004311079 1108 5 0.04227808174793779 1108 6 0.0972080004045921 1108 7 0.0894843250552313 1108 8 0.09949909743899354 1108 9 0.1623622358615293 1108 10-11 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2316 9 -0.3818561181531521 2316 10-11 -0.36762429033017696 2316 12-13 -0.2395102803539757 2316 14-15 -0.3211952550817543 2316 16-17 -0.31697486916912254 2316 18-19 -0.39787967755513165 2316 20-21 -0.31306307121325716 2316 22-23 -0.4232147231473675 2316 24-25 -0.47415655901217235 2316 26-27 -0.6632548029482876 2316 28-29 -0.5101944089137049 2316 30-31 -0.4285539540721217 2316 32-33 -0.4906729565751675 2316 34-35 -0.3818830363458119 2316 36-37 -0.38748427266447294 2316 38-39 -0.37393355725553334 2316 40-41 -0.5618688480201754 2316 42-43 -0.4268617336943592 2316 44-45 -0.6095225256291243 2316 46-47 -0.5860746343254846 2316 48-49 -0.41880212444062437 2316 50-51 -0.19229032784905797 2316 52-53 -0.3246015890575933 2316 54-55 -0.26781336995756533 2316 56-57 -0.31897101194720534 2316 58-59 -0.15062468172875043 2316 60-61 -0.3236713978706902 2316 62-63 -0.27973194801572276 2316 64-65 -0.2649338825565337 2316 66-67 -0.0489250549645277 2316 68-69 -0.1276403470781844 2316 70-71 0.026015580384409986 2316 72-73 0.2667996214927868 2316 74-75 0.012443440033973019 2316 76-77 0.024338565862283446 2316 78-79 0.020645336527280733 2316 80-81 0.019193128090524425 2316 82-83 0.051975463651050546 2316 84-85 0.06902247206231138 2316 86-87 0.12998754297489157 2316 88-89 0.2922294420008349 2316 90-91 0.16882577641210972 2316 92-93 0.14601877218408887 2316 94-95 0.23086993807777745 2316 96-97 0.38748179360696255 2316 98-99 0.09906278185383144 2316 100-101 0.33606906968685024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 12.0 5 41.0 6 108.0 7 226.0 8 547.0 9 1056.0 10 1845.0 11 3208.0 12 5145.0 13 7420.0 14 9763.0 15 13827.0 16 18806.0 17 27042.0 18 25511.0 19 15268.0 20 12297.0 21 12360.0 22 12530.0 23 13507.0 24 15498.0 25 18229.0 26 22083.0 27 27455.0 28 34526.0 29 43033.0 30 54610.0 31 69175.0 32 89297.0 33 119045.0 34 166271.0 35 241644.0 36 375837.0 37 580661.0 38 473289.0 39 42738.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6858365706017 19.914283061757953 15.493017332590103 21.906863035050243 2 15.503196060964985 20.835850700392534 40.32984523821177 23.33110800043071 3 19.38780504517556 25.001164873674835 30.140587527041713 25.47044255410789 4 12.942295395981315 17.196773118667334 37.280792945435664 32.58013853991568 5 13.127795499632292 38.304894390713244 34.241585991546245 14.32572411810822 6 31.577043178636803 37.082999696545514 17.068159793259394 14.27179733155829 7 28.242606967706568 33.64738588642971 20.457805143064107 17.652202002799612 8 26.400853588104585 35.434909012598254 19.377546325753496 18.78669107354366 9 26.395841694646478 17.234766095323863 20.491831200994547 35.87756100903511 10-11 24.677388725197492 27.520169934512563 26.868114764529103 20.93432657576084 12-13 25.79077216939324 24.711707384978105 27.821189367705312 21.67633107792334 14-15 22.65213348081874 25.975743218771107 26.655931556329964 24.71619174408019 16-17 21.435711698659908 29.42329943126756 27.325959063010856 21.815029807061677 18-19 21.311980578912852 27.76802372818309 30.22165881926838 20.69833687363568 20-21 22.019109820286246 26.556698568574415 30.685543511989888 20.738648099149444 22-23 21.82931351308809 26.362364505555647 30.65558499651707 21.152736984839187 24-25 21.77358379748818 26.525289505369187 30.687275468151963 21.01385122899067 26-27 21.277852478286167 26.933472780083186 30.742056664664503 21.046618076966148 28-29 21.194430995438125 27.12592244249008 30.654917771372286 21.024728790699506 30-31 21.37425024672822 26.74449724120554 30.56446287447529 21.316789637590947 32-33 21.347024677701967 26.81425648756326 30.47432872930881 21.36439010542596 34-35 21.840774494160947 26.771714126295798 30.48656479732177 20.900946582221483 36-37 21.869233127597234 26.607774169815094 30.366350694755784 21.156642007831884 38-39 21.433107863386745 27.036952925399138 30.3466820677976 21.183257143416505 40-41 21.820744008335414 26.770912827050775 30.275149250780075 21.133193913833736 42-43 21.445241209690685 26.971954820669076 29.981044732863893 21.601759236776353 44-45 21.613144473702242 27.124171618195525 29.899625086876082 21.36305882122615 46-47 22.175435848742623 27.361786270152805 29.225133862584062 21.237644018520513 48-49 21.942480691484676 27.533580665054767 29.058899536987187 21.46503910647337 50-51 22.108793327916835 27.80473193222197 28.906271719020722 21.180203020840473 52-53 22.291688283720156 27.843339174016464 28.525426549330934 21.339545992932447 54-55 22.041817986041092 28.033242949577613 28.465029317618963 21.45990974676234 56-57 22.174731051225073 28.20537016552953 28.22702311148526 21.392875671760134 58-59 22.186536409643978 27.682488718345294 28.423230909286683 21.707743962724045 60-61 21.928071497792615 27.996084458235853 28.749062717190206 21.326781326781326 62-63 21.83891461182298 27.94794287224566 29.008408625938507 21.204733889992855 64-65 21.89827422496745 27.990504811221946 28.705208649431757 21.40601231437885 66-67 21.869925702595026 27.979972003876384 28.45545581800562 21.69464647552297 68-69 21.90081932711415 27.909629296083477 28.667404093698917 21.522147283103457 70-71 22.171853128028427 27.980402713470443 28.301672915218735 21.546071243282398 72-73 22.036962714253548 27.953835762600704 28.263476805309473 21.74572471783627 74-75 21.82029621073446 28.488033125483327 28.00088099689693 21.690789666885284 76-77 22.058850592715135 27.999784645202975 28.30762453870024 21.633740223381658 78-79 22.02919036385172 28.087414469884592 28.15010229352859 21.733292872735106 80-81 22.134949146901338 28.300615718942414 27.98721575614006 21.577219378016192 82-83 22.035659307194294 28.21269425289247 28.140099952249862 21.611546487663375 84-85 21.883685794985237 28.41419959172542 27.91443814671455 21.787676466574794 86-87 21.953111387374335 28.453830868173497 27.889092279530526 21.70396546492164 88-89 22.062785712188102 28.31226445569075 27.75559188308192 21.869357949039223 90-91 22.0442450100669 28.190838545283807 27.83466439333406 21.930252051315236 92-93 21.97456288337703 28.047981364726777 27.96910096906005 22.008354782836143 94-95 21.913309905341777 28.032616462895348 27.974157424356626 22.079916207406246 96-97 21.87597521462063 28.155584051998396 27.91998590404965 22.048454829331323 98-99 21.63199732488982 28.353113760509117 27.62581818060792 22.389070733993144 100-101 21.536052869736295 28.440059257588413 27.935104873635115 22.088782999040177 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 732.0 1 510.0 2 366.0 3 461.0 4 416.5 5 428.5 6 787.0 7 1711.5 8 2839.5 9 3499.0 10 3442.0 11 3236.5 12 3340.5 13 3652.5 14 4014.5 15 4333.0 16 4588.5 17 4729.5 18 5039.5 19 5454.0 20 5868.5 21 6685.5 22 7794.5 23 9137.0 24 10990.0 25 13478.5 26 16783.0 27 20857.5 28 24403.5 29 28197.0 30 33102.5 31 38383.0 32 45205.5 33 51543.0 34 56791.5 35 62266.0 36 69532.0 37 75787.5 38 78996.0 39 80997.5 40 82283.0 41 84472.0 42 84949.0 43 87611.0 44 93764.5 45 96636.5 46 94320.0 47 95028.5 48 99440.5 49 97424.0 50 92254.5 51 87230.5 52 82050.5 53 79019.0 54 78956.5 55 72827.5 56 61912.0 57 54517.5 58 49741.0 59 44137.5 60 35168.0 61 28338.5 62 22965.0 63 16254.0 64 11037.0 65 8197.5 66 6013.0 67 4613.5 68 3616.5 69 2785.5 70 2132.5 71 1327.5 72 876.0 73 647.5 74 446.5 75 314.5 76 224.0 77 135.0 78 85.5 79 57.5 80 36.0 81 27.0 82 17.0 83 9.0 84 4.5 85 1.5 86 0.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043227581076186655 2 0.0 3 0.0 4 1.5662167056589366E-4 5 0.010454496510273402 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005462180760985542 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.788854410368354E-5 22-23 0.00148790587037599 24-25 0.0 26-27 9.005746057538886E-4 28-29 0.0015466389968382 30-31 5.873312646221013E-5 32-33 0.0 34-35 0.0 36-37 2.3493250584884052E-4 38-39 0.0 40-41 7.831083528294683E-5 42-43 0.021339702614603013 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.153547970281038E-4 84-85 2.5451021466957725E-4 86-87 0.0 88-89 0.0 90-91 0.010121675460320879 92-93 0.002290591932026195 94-95 0.0 96-97 0.0 98-99 4.502873028769443E-4 100-101 0.012842976986403283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2553925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.693289728667494 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5038995489637 32.920590086406314 2 15.35207457493851 14.336777321274274 3 5.690500203857655 7.971245241593008 4 2.8069404391760044 5.242611327102334 5 1.5162915161887809 3.540031953926163 6 0.9505361082219665 2.6630194739260995 7 0.6250994571507442 2.0431565043380733 8 0.4326591720257611 1.61618240585314 9 0.31711209989262135 1.3326306441077085 >10 1.5881959544163267 13.514045718747274 >50 0.13438769390443508 4.325227072967156 >100 0.07547437961876981 6.577366542253219 >500 0.005479942677793174 1.735945254252379 >1k 0.0010959885355576265 0.8689174020836785 >5k 8.430681042750973E-5 0.30154067412499336 >10k+ 1.6861362085501946E-4 1.0107123770442825 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14249 0.5579255459733548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11426 0.44738980197147526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7660 0.29993049913368636 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3777 0.1478900124318451 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2935 0.11492115077772448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.915541764147342E-5 0.0 0.0 0.0 0.0 7 3.915541764147342E-5 0.0 0.0 0.0 0.0 8 3.915541764147342E-5 0.0 0.0 0.0 0.0 9 3.915541764147342E-5 0.0 0.0 0.0 0.0 10-11 3.915541764147342E-5 0.0 0.0 0.0 0.0 12-13 7.831083528294684E-5 0.0 0.0 0.0 0.0 14-15 1.1746625292442026E-4 3.915541764147342E-5 0.0 3.915541764147342E-5 3.915541764147342E-5 16-17 1.3704396174515698E-4 3.915541764147342E-5 0.0 7.831083528294684E-5 7.831083528294684E-5 18-19 1.7619937938663039E-4 3.915541764147342E-5 0.0 7.831083528294684E-5 7.831083528294684E-5 20-21 1.957770882073671E-4 3.915541764147342E-5 0.0 7.831083528294684E-5 7.831083528294684E-5 22-23 1.957770882073671E-4 3.915541764147342E-5 0.0 1.5662167056589368E-4 7.831083528294684E-5 24-25 1.957770882073671E-4 3.915541764147342E-5 0.0 2.9366563231105066E-4 7.831083528294684E-5 26-27 1.957770882073671E-4 3.915541764147342E-5 0.0 5.285981381598912E-4 7.831083528294684E-5 28-29 3.3282104995252407E-4 3.915541764147342E-5 0.0 0.0015857944144796734 1.1746625292442026E-4 30-31 4.6986501169768105E-4 3.915541764147342E-5 0.0 0.0043070959405620765 1.1746625292442026E-4 32-33 4.894427205184177E-4 3.915541764147342E-5 0.0 0.006519377037305324 1.1746625292442026E-4 34-35 5.090204293391544E-4 3.915541764147342E-5 0.0 0.00851630333702047 1.5662167056589368E-4 36-37 5.090204293391544E-4 3.915541764147342E-5 0.0 0.011433381951310238 1.5662167056589368E-4 38-39 5.090204293391544E-4 3.915541764147342E-5 0.0 0.017169650635786092 1.5662167056589368E-4 40-41 5.090204293391544E-4 3.915541764147342E-5 0.0 0.023571561420166996 1.5662167056589368E-4 42-43 6.460643910843114E-4 3.915541764147342E-5 0.0 0.029503607192850223 1.5662167056589368E-4 44-45 7.047975175465215E-4 3.915541764147342E-5 0.0 0.03381070313341229 1.5662167056589368E-4 46-47 8.026860616502051E-4 3.915541764147342E-5 0.0 0.0388813297179831 1.7619937938663039E-4 48-49 8.222637704709418E-4 3.915541764147342E-5 0.0 0.04608592656401421 1.957770882073671E-4 50-51 8.222637704709418E-4 3.915541764147342E-5 0.0 0.05446518593928952 1.957770882073671E-4 52-53 8.222637704709418E-4 3.915541764147342E-5 0.0 0.0713020155251231 2.3493250584884052E-4 54-55 8.222637704709418E-4 3.915541764147342E-5 0.0 0.08347935041162133 2.3493250584884052E-4 56-57 8.222637704709418E-4 3.915541764147342E-5 0.0 0.11456875201895123 2.3493250584884052E-4 58-59 8.222637704709418E-4 3.915541764147342E-5 0.0 0.16259287175621837 2.3493250584884052E-4 60-61 8.222637704709418E-4 3.915541764147342E-5 0.0 0.2083068218526386 2.3493250584884052E-4 62-63 8.222637704709418E-4 3.915541764147342E-5 0.0 0.23405150895190735 2.3493250584884052E-4 64-65 9.005746057538886E-4 3.915541764147342E-5 0.0 0.27765106649568805 2.3493250584884052E-4 66-67 9.005746057538886E-4 3.915541764147342E-5 0.0 0.3590943351899527 2.3493250584884052E-4 68-69 9.397300233953621E-4 3.915541764147342E-5 0.0 0.47409379680296015 2.3493250584884052E-4 70-71 9.397300233953621E-4 3.915541764147342E-5 0.0 0.5808901984200789 2.9366563231105066E-4 72-73 9.397300233953621E-4 3.915541764147342E-5 0.0 0.7015280401734585 3.1324334113178736E-4 74-75 9.397300233953621E-4 3.915541764147342E-5 0.0 0.8463835077380895 3.5239875877326077E-4 76-77 9.397300233953621E-4 3.915541764147342E-5 0.0 0.9608347935041162 3.5239875877326077E-4 78-79 9.397300233953621E-4 3.915541764147342E-5 0.0 1.1107021545268556 3.5239875877326077E-4 80-81 9.98463149857572E-4 3.915541764147342E-5 0.0 1.2704562585040673 3.5239875877326077E-4 82-83 0.0010180408586783088 3.915541764147342E-5 0.0 1.393247648227728 3.5239875877326077E-4 84-85 0.0010376185674990455 3.915541764147342E-5 0.0 1.568057010288086 3.5239875877326077E-4 86-87 0.0010571962763197822 3.915541764147342E-5 0.0 1.7965680276437248 3.5239875877326077E-4 88-89 0.0010571962763197822 3.915541764147342E-5 0.0 2.1177599185567315 3.915541764147342E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16725 0.0 31.14689 1 GGTATCA 11355 0.0 30.76596 1 ATCAACG 20270 0.0 25.591309 3 TCAACGC 20430 0.0 25.367636 4 TATCAAC 20525 0.0 25.296509 2 CAACGCA 20615 0.0 25.139984 5 AACGCAG 21080 0.0 24.650135 6 GAGTACT 12740 0.0 23.340117 12-13 GTACATG 11595 0.0 23.324469 1 TACATGG 11560 0.0 23.17634 2 ACATGGG 11330 0.0 22.724428 3 GTACTTT 13445 0.0 22.026638 14-15 ACGCAGA 23535 0.0 21.999376 7 GCGGTCG 240 5.9353624E-9 21.771057 9 CGCAGAG 23625 0.0 21.75472 8 AGTACTT 13465 0.0 21.607166 12-13 GGACCGA 975 0.0 21.434858 6 CGAGCGG 225 7.163908E-8 21.110088 6 AGAGTAC 19305 0.0 20.619223 10-11 GCAGAGT 25270 0.0 20.000206 9 >>END_MODULE