FastQCFastQC Report
Thu 26 May 2016
SRR937820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170473
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112250.5171683775840565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81630.37609313730232996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.2604962144196219No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30900.14236528166901868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22860.10532266469106043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72700.036.700211
GTATCAA116850.036.1430131
ATCAACG140850.029.8349113
TCAACGC142800.029.363644
CAACGCA143800.029.0919745
TATCAAC144700.028.9760672
AACGCAG147250.028.424266
ACGCAGA166950.024.900057
GTACATG97150.024.82021
CGCAGAG167450.024.7689538
TACATGG98300.023.9865362
GAGTACT96050.023.31646212-13
ACATGGG95350.023.1825713
GCAGAGT179850.022.5577589
AGGACCG6550.022.4993445
GACCGCG1100.002874913921.5951337
GTACTTT104700.021.38839514-15
TAAGGTG8250.021.3204925
AGAGTAC145750.020.922810-11
CCTATAC5800.020.4899733
Produced by FastQC (version 0.11.5)