##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2170473 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770885885242524 33.0 31.0 34.0 30.0 34.0 2 31.875152098183207 33.0 31.0 34.0 30.0 34.0 3 31.91694943913147 33.0 31.0 34.0 30.0 34.0 4 35.51693985596688 37.0 35.0 37.0 33.0 37.0 5 35.52432672509633 37.0 35.0 37.0 33.0 37.0 6 35.589816136851276 37.0 35.0 37.0 33.0 37.0 7 35.50925305221489 37.0 35.0 37.0 33.0 37.0 8 35.516811773286285 37.0 35.0 37.0 33.0 37.0 9 37.08833097670416 39.0 37.0 39.0 33.0 39.0 10-11 37.00974557158739 39.0 37.0 39.0 33.0 39.0 12-13 36.90973327012131 39.0 37.0 39.0 33.0 39.0 14-15 38.03701128740141 40.0 38.0 41.0 33.0 41.0 16-17 37.94729927531925 40.0 38.0 41.0 33.0 41.0 18-19 37.88865307239482 40.0 38.0 41.0 33.0 41.0 20-21 37.90171911836728 40.0 38.0 41.0 33.0 41.0 22-23 37.90377627365095 40.0 38.0 41.0 33.0 41.0 24-25 37.83797103212065 40.0 38.0 41.0 33.0 41.0 26-27 37.724739031538284 40.0 38.0 41.0 32.5 41.0 28-29 37.57571851849804 40.0 37.0 41.0 32.0 41.0 30-31 37.32458408835309 40.0 37.0 41.0 32.0 41.0 32-33 37.16566711495605 39.5 36.5 41.0 31.5 41.0 34-35 37.128153402507195 40.0 36.5 41.0 31.0 41.0 36-37 36.95926463955092 40.0 36.0 41.0 31.0 41.0 38-39 36.78404315557024 39.5 36.0 41.0 30.5 41.0 40-41 36.54402588744481 39.0 35.5 41.0 30.0 41.0 42-43 36.26197308144354 39.0 35.0 41.0 30.0 41.0 44-45 35.98405416699494 39.0 35.0 41.0 29.5 41.0 46-47 35.5864267834707 39.0 35.0 40.0 27.5 41.0 48-49 35.19378034188861 39.0 35.0 40.0 26.0 41.0 50-51 34.315964308240645 38.0 34.0 39.5 24.0 40.5 52-53 34.411346512949024 38.0 34.0 39.5 24.0 40.5 54-55 34.67042736767516 38.0 34.0 40.0 24.0 41.0 56-57 34.41973086050829 38.0 34.0 40.0 23.0 41.0 58-59 34.135270745132516 38.0 34.0 40.0 22.5 41.0 60-61 33.7828031032867 38.0 33.5 40.0 21.5 41.0 62-63 33.40464175320311 37.0 33.0 40.0 19.5 41.0 64-65 32.96412993849728 36.5 32.5 39.5 18.0 41.0 66-67 32.443435140635245 36.0 32.0 39.0 13.0 40.5 68-69 31.970502973315035 35.5 31.0 39.0 10.0 40.0 70-71 31.489889761356164 35.0 31.0 38.0 7.5 40.0 72-73 31.00217786629919 35.0 30.5 37.5 6.0 39.5 74-75 30.4510473062784 34.5 30.0 37.0 2.0 39.0 76-77 29.89552715007282 34.0 29.0 36.5 2.0 39.0 78-79 29.349631393710034 34.0 29.0 36.0 2.0 38.5 80-81 28.77866253116256 34.0 28.5 35.5 2.0 37.0 82-83 28.215154024030706 34.0 27.0 35.0 2.0 37.0 84-85 27.648767572782525 33.0 26.0 35.0 2.0 36.0 86-87 27.05127085202166 33.0 25.5 35.0 2.0 36.0 88-89 26.4744260813196 32.0 25.0 35.0 2.0 35.5 90-91 25.884961711110897 32.0 23.5 34.5 2.0 35.0 92-93 25.184153638400478 32.0 19.5 34.0 2.0 35.0 94-95 24.392742273228002 31.0 14.0 34.0 2.0 35.0 96-97 23.48862621189022 31.0 2.0 34.0 2.0 35.0 98-99 22.276002742259408 30.5 2.0 34.0 2.0 35.0 100-101 19.025715823233 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18678224933788812 1101 2 0.05835870832224899 1101 3 0.06798586431942155 1101 4 0.135985500395023 1101 5 0.037400466793570786 1101 6 0.08911841106520058 1101 7 0.17392411918936546 1101 8 0.24623194112550806 1101 9 0.3620939777109129 1101 10-11 0.4300211682629609 1101 12-13 0.3450201171244274 1101 14-15 0.537512049770136 1101 16-17 0.49829204797093496 1101 18-19 0.5737601064971471 1101 20-21 0.4695818600786268 1101 22-23 0.45960457246049913 1101 24-25 0.3876148249260538 1101 26-27 0.4367437104541949 1101 28-29 0.4553036380536426 1101 30-31 0.4670644630032399 1101 32-33 0.40193303878935893 1101 34-35 0.4666816345292588 1101 36-37 0.5555622246259517 1101 38-39 0.5241174265832953 1101 40-41 0.5760017671284601 1101 42-43 0.4052434718385527 1101 44-45 0.5121047349574539 1101 46-47 0.6089861177677633 1101 48-49 0.8694117126530045 1101 50-51 0.6602895483130382 1101 52-53 0.9014716510367577 1101 54-55 0.9444068215130699 1101 56-57 1.0965350273770085 1101 58-59 1.0825819445646374 1101 60-61 0.9006665616335638 1101 62-63 1.0336733668795333 1101 64-65 1.134033043376462 1101 66-67 1.2434713892291498 1101 68-69 1.257774279220449 1101 70-71 1.2068587989697335 1101 72-73 1.25655478058167 1101 74-75 1.2674642843529398 1101 76-77 1.2199825256003471 1101 78-79 1.5979209466220148 1101 80-81 1.7529691738198316 1101 82-83 1.7097079362500835 1101 84-85 1.7991539808505834 1101 86-87 2.1268301336670596 1101 88-89 2.2113944022348946 1101 90-91 2.206721590367227 1101 92-93 2.260761181652132 1101 94-95 2.465388260675315 1101 96-97 2.7272290258118517 1101 98-99 2.9916871083717247 1101 100-101 3.8281572700954456 1102 1 0.1247974741782798 1102 2 0.18410542534224916 1102 3 0.13657208669716425 1102 4 0.21214337393509908 1102 5 0.0754434211784698 1102 6 0.16892404016273588 1102 7 0.11901433924310822 1102 8 0.18637408805248157 1102 9 0.3617452126659799 1102 10-11 0.34209342353322825 1102 12-13 0.30818669906193463 1102 14-15 0.5397909891810784 1102 16-17 0.4708336918047422 1102 18-19 0.3957198425382984 1102 20-21 0.42988594424439697 1102 22-23 0.5216331702038417 1102 24-25 0.4510137243272325 1102 26-27 0.3861859009561641 1102 28-29 0.3803009146162353 1102 30-31 0.519700420299742 1102 32-33 0.4897293157678888 1102 34-35 0.5691583945300493 1102 36-37 0.5159977765429247 1102 38-39 0.6661818251492875 1102 40-41 0.6621577920322252 1102 42-43 0.7703976679128459 1102 44-45 0.8909570784340772 1102 46-47 0.8398640384480629 1102 48-49 0.8805853161073074 1102 50-51 0.5970306996173207 1102 52-53 0.7247109900172362 1102 54-55 0.821881683360516 1102 56-57 0.9053941216772685 1102 58-59 0.8230981102288624 1102 60-61 0.8941641485029663 1102 62-63 0.9625024373455915 1102 64-65 0.9589042165342647 1102 66-67 1.02859399342951 1102 68-69 0.9553232680824237 1102 70-71 1.0018901762697965 1102 72-73 1.0337832126079753 1102 74-75 1.0296626430279936 1102 76-77 1.0831192322547771 1102 78-79 1.3766763192212643 1102 80-81 1.3572675833323906 1102 82-83 1.5011158213441291 1102 84-85 1.711714556700791 1102 86-87 1.8249643314579416 1102 88-89 1.6924946544417026 1102 90-91 1.6687465568606932 1102 92-93 1.952592107356761 1102 94-95 2.129454814883271 1102 96-97 2.4695340772105396 1102 98-99 2.947449406754828 1102 100-101 3.6277820134321033 1103 1 -0.09187262108954286 1103 2 -0.12455433446650943 1103 3 -0.012325026893574176 1103 4 -0.07980816219644993 1103 5 -0.12214118842208421 1103 6 -0.061075037661787235 1103 7 -0.07614920503196032 1103 8 -0.21599767635296274 1103 9 -0.17464507327935763 1103 10-11 -0.07286710026244947 1103 12-13 -0.02105264803783058 1103 14-15 0.1730561642880204 1103 16-17 0.17981798644689917 1103 18-19 0.22297128077737227 1103 20-21 0.17450088277785625 1103 22-23 -0.15489170015955978 1103 24-25 -0.04562911356141086 1103 26-27 0.03629186246597982 1103 28-29 0.03642584456636655 1103 30-31 0.05608520866046263 1103 32-33 0.16389394891938736 1103 34-35 -0.02696908913679863 1103 36-37 0.12429912930342368 1103 38-39 0.24590389564605886 1103 40-41 0.2998800895260416 1103 42-43 0.19533648511637836 1103 44-45 0.37281389650303964 1103 46-47 0.32636755690220554 1103 48-49 0.40989391503901373 1103 50-51 0.18348021760328947 1103 52-53 0.328263493723675 1103 54-55 0.5299345807649516 1103 56-57 0.5898127255035845 1103 58-59 0.538850995574073 1103 60-61 0.7455546827263149 1103 62-63 0.7374257371732114 1103 64-65 0.7527014336923941 1103 66-67 0.8387966237577373 1103 68-69 1.0669816056128312 1103 70-71 1.0725040791292457 1103 72-73 1.006554562743819 1103 74-75 0.8596564006093388 1103 76-77 0.890564203206246 1103 78-79 1.166355579494656 1103 80-81 1.239750860034686 1103 82-83 1.372469060662855 1103 84-85 1.5555759714598594 1103 86-87 1.6444804021391484 1103 88-89 1.7118437581580714 1103 90-91 1.8327984717935557 1103 92-93 1.9084331136630226 1103 94-95 2.0126792901299524 1103 96-97 2.154056071095809 1103 98-99 2.412388117553501 1103 100-101 3.088147531872064 1104 1 0.3578190170521829 1104 2 0.3171473378019165 1104 3 0.22503230897008564 1104 4 0.29981733019752 1104 5 0.29426491842450275 1104 6 0.406162902073234 1104 7 0.4216966062965213 1104 8 0.4016871278465075 1104 9 0.438632251091164 1104 10-11 0.39105864857641137 1104 12-13 0.44967547134626074 1104 14-15 0.5740746315889282 1104 16-17 0.6997202087685324 1104 18-19 0.6569251516671315 1104 20-21 0.6149464826532878 1104 22-23 0.4786651586013235 1104 24-25 0.5240040609957433 1104 26-27 0.5060955414737194 1104 28-29 0.5030655562744855 1104 30-31 0.6003085746523027 1104 32-33 0.4680190038687968 1104 34-35 0.5827722034796352 1104 36-37 0.5992507179111115 1104 38-39 0.7721558172621812 1104 40-41 0.9205663570356535 1104 42-43 0.77448625116741 1104 44-45 0.7551599758039558 1104 46-47 0.8451695007561781 1104 48-49 0.8592823959234934 1104 50-51 0.7950646271993662 1104 52-53 0.7088781183945159 1104 54-55 0.9299749271763105 1104 56-57 0.9941981825578878 1104 58-59 0.9847641793876747 1104 60-61 1.0309426577751992 1104 62-63 1.0990996573045209 1104 64-65 1.0412230086993048 1104 66-67 1.3348637519277062 1104 68-69 1.3138829754122128 1104 70-71 1.3923315444541373 1104 72-73 1.3577199202310872 1104 74-75 1.316389820944103 1104 76-77 1.5335225500529042 1104 78-79 1.8173234202218183 1104 80-81 1.9107515972814788 1104 82-83 1.9230314586677295 1104 84-85 1.9170167598751888 1104 86-87 2.0067522922179712 1104 88-89 2.022695824470709 1104 90-91 2.183235443214702 1104 92-93 2.31338350650287 1104 94-95 2.4128540098772397 1104 96-97 2.6181978323844177 1104 98-99 3.153683314782075 1104 100-101 3.3284790606569494 1105 1 0.24877943789599044 1105 2 0.20905474199085106 1105 3 0.11894389277217243 1105 4 0.1095847385406401 1105 5 0.059682646597046585 1105 6 0.03708440927734813 1105 7 0.10523545847131999 1105 8 0.08346766000536121 1105 9 0.345994050444304 1105 10-11 0.21638473046461115 1105 12-13 0.24450394188761493 1105 14-15 0.24164617461223514 1105 16-17 0.3080378932798027 1105 18-19 0.30285002884330936 1105 20-21 0.2902948190739991 1105 22-23 0.2471264590290403 1105 24-25 0.1961354517556586 1105 26-27 0.2679945101350967 1105 28-29 0.4212587425724763 1105 30-31 0.35826870300797253 1105 32-33 0.3518736584572508 1105 34-35 0.3919008007658533 1105 36-37 0.5152375262720312 1105 38-39 0.45887643174162207 1105 40-41 0.5011612524817366 1105 42-43 0.4378781810380872 1105 44-45 0.6610228794057988 1105 46-47 0.7455777124667193 1105 48-49 0.7113304487578773 1105 50-51 0.794612754215521 1105 52-53 0.7668026394981666 1105 54-55 0.9838508189472179 1105 56-57 0.8726151010893446 1105 58-59 0.8784722931782767 1105 60-61 1.0208034927652534 1105 62-63 1.1400632573672596 1105 64-65 1.0835210329207143 1105 66-67 1.2603750763055288 1105 68-69 1.0947326322685171 1105 70-71 1.0612294576148287 1105 72-73 1.078138135883865 1105 74-75 1.1918301427658378 1105 76-77 1.35943699939099 1105 78-79 1.5939095325457444 1105 80-81 1.7068131808716238 1105 82-83 1.6527223961981967 1105 84-85 1.9830474414573374 1105 86-87 2.080593678866098 1105 88-89 2.1782469526695323 1105 90-91 2.0748630761378486 1105 92-93 2.1325944667541528 1105 94-95 2.13328621409331 1105 96-97 2.09355973347256 1105 98-99 2.598056733120657 1105 100-101 2.9606374959725628 1106 1 -0.04886806849617287 1106 2 0.025843353763054466 1106 3 0.1290121053547857 1106 4 0.09957668750949011 1106 5 -0.02867874738149112 1106 6 0.15624851760352954 1106 7 0.21998123167996653 1106 8 0.15783618489720652 1106 9 0.18898655679222998 1106 10-11 -0.11523989684342695 1106 12-13 -0.13248691886493447 1106 14-15 -0.10853298294909308 1106 16-17 0.0022730010839993042 1106 18-19 0.2918723151592104 1106 20-21 0.39164668452445284 1106 22-23 0.25383712620294574 1106 24-25 0.12703637098878318 1106 26-27 0.18518390400745943 1106 28-29 0.42543377729807474 1106 30-31 0.4986901877596779 1106 32-33 0.4166756119382171 1106 34-35 0.4007666670479537 1106 36-37 0.22569148056695099 1106 38-39 0.09191574023766691 1106 40-41 0.39584403300708715 1106 42-43 0.4042452195857109 1106 44-45 0.3331256141022081 1106 46-47 0.477299659931532 1106 48-49 0.6793236186523259 1106 50-51 0.7097186120042309 1106 52-53 0.6820491691498489 1106 54-55 0.7563100993089051 1106 56-57 0.6457759713084883 1106 58-59 0.49969251099847867 1106 60-61 0.7337851169502017 1106 62-63 0.5389803494409762 1106 64-65 0.8363915351811926 1106 66-67 1.0524142874814189 1106 68-69 0.9021829326339059 1106 70-71 0.9925488535220737 1106 72-73 0.8567967102320431 1106 74-75 1.084817783105926 1106 76-77 1.419554314416338 1106 78-79 1.349466071133186 1106 80-81 1.2955939362529882 1106 82-83 1.2033191457049597 1106 84-85 1.278292024705884 1106 86-87 1.6339180469486259 1106 88-89 1.7259400225896435 1106 90-91 2.1555556417626 1106 92-93 2.101626308139995 1106 94-95 2.164084504797465 1106 96-97 2.168876687528595 1106 98-99 2.4905259224437515 1106 100-101 3.0088366307078935 1107 1 0.07685106134556463 1107 2 0.22662701885537828 1107 3 0.2253037983135897 1107 4 0.19816487931144167 1107 5 0.18347268259218907 1107 6 0.22175323106701939 1107 7 0.30777689873544034 1107 8 0.32761329129591843 1107 9 0.3824970194701436 1107 10-11 0.3297057979383027 1107 12-13 0.41342202428668173 1107 14-15 0.5035755828704893 1107 16-17 0.4186346789367761 1107 18-19 0.6109873376907728 1107 20-21 0.5073030956633957 1107 22-23 0.5521703639285036 1107 24-25 0.43760150856837043 1107 26-27 0.4904891796446975 1107 28-29 0.5596967369324659 1107 30-31 0.5667390242130068 1107 32-33 0.6693983621278434 1107 34-35 0.8008898417361152 1107 36-37 0.8344668405302329 1107 38-39 0.705380661743682 1107 40-41 0.6911680158996703 1107 42-43 0.8301896650924689 1107 44-45 0.9175501508825121 1107 46-47 0.866483551932987 1107 48-49 1.0190768549756442 1107 50-51 0.8866393722772941 1107 52-53 1.0807175121395645 1107 54-55 1.0775224378114245 1107 56-57 1.156623629873991 1107 58-59 1.1004293282792688 1107 60-61 1.349566933625475 1107 62-63 1.4338689982481085 1107 64-65 1.369919111651484 1107 66-67 1.3737861316280018 1107 68-69 1.5798319054876728 1107 70-71 1.6990455614275604 1107 72-73 1.6583425286664983 1107 74-75 1.7732903532342341 1107 76-77 1.7928803815584935 1107 78-79 1.9637951276118883 1107 80-81 2.0869476467908754 1107 82-83 2.290590085401945 1107 84-85 2.2344075762671594 1107 86-87 2.328608787653959 1107 88-89 2.3899717388767456 1107 90-91 2.44708033087797 1107 92-93 2.6113991759271826 1107 94-95 2.87557018593915 1107 96-97 2.8470371619981307 1107 98-99 2.999987746257734 1107 100-101 3.448844502415003 1108 1 0.12876602665656023 1108 2 0.1457613281741743 1108 3 0.03447336826623726 1108 4 0.04711819206604417 1108 5 0.06394966389682821 1108 6 0.18635961769444975 1108 7 0.15575693375538435 1108 8 0.26912336103494994 1108 9 0.3048812039325952 1108 10-11 0.28986925444316824 1108 12-13 0.3736834102823181 1108 14-15 0.45468108798207396 1108 16-17 0.4233076154977127 1108 18-19 0.4581434712960828 1108 20-21 0.3756386252648767 1108 22-23 0.3703416247067608 1108 24-25 0.4952467781678038 1108 26-27 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-1.6797764194549387 2316 76-77 -1.6656812941702306 2316 78-79 -1.9490450577842289 2316 80-81 -2.099503858657105 2316 82-83 -2.1604932425586227 2316 84-85 -2.0746643327827243 2316 86-87 -2.375650559161425 2316 88-89 -2.288323734391973 2316 90-91 -2.561382127841309 2316 92-93 -2.4571130345704795 2316 94-95 -2.447931708855002 2316 96-97 -2.3461052233650292 2316 98-99 -2.7439243768429904 2316 100-101 -2.7354614799766637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8933.0 3 1709.0 4 1571.0 5 1790.0 6 2123.0 7 2607.0 8 3124.0 9 3862.0 10 4872.0 11 6462.0 12 8603.0 13 11227.0 14 12997.0 15 16354.0 16 20367.0 17 26231.0 18 22784.0 19 16302.0 20 16014.0 21 17202.0 22 18585.0 23 20761.0 24 23234.0 25 26131.0 26 30057.0 27 35205.0 28 40914.0 29 49039.0 30 59998.0 31 73706.0 32 92712.0 33 120185.0 34 161443.0 35 221636.0 36 311480.0 37 398691.0 38 260799.0 39 20763.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.149765610515374 19.072169953297795 15.242043726503834 22.536020709682997 2 14.819904661388971 19.980120846033135 41.55949236533282 23.64048212724507 3 18.925041347940553 24.6889373290455 30.246313113085172 26.139708209928774 4 12.193968978936988 16.512528661166556 38.17080530369053 33.12269705620592 5 12.92488180687722 37.754148138461204 34.867144506832815 14.453825547828764 6 31.109936257794622 36.57260401107235 17.831287472498044 14.48617225863498 7 27.84241901557361 33.242812003109925 21.09750264546356 17.81726633585291 8 25.843505418057795 34.9097082712127 20.226815519160706 19.019970791568802 9 26.048726881511904 17.044019494446484 20.955606500303592 35.95164712373802 10-11 24.179065953841846 27.04793421399843 27.660447341670864 21.11255249048886 12-13 25.322444392266924 24.36810364949449 28.434741272212676 21.874710686025914 14-15 22.376495184658133 25.57394867267588 27.127056874501914 24.922499268164074 16-17 21.144878393691926 28.995623470240222 27.84275333329794 22.016744802769917 18-19 21.016138134956496 27.478455364778632 30.574996797302116 20.930409702962756 20-21 21.678591403064093 26.319093896998623 30.949763301849753 21.052551398087534 22-23 21.420313809318248 26.13089315847671 30.955509438056477 21.493283594148565 24-25 21.32478154031535 26.21373096016076 31.08689103389054 21.37459646563336 26-27 20.945207522359446 26.64189747920344 30.969064063608826 21.443830934828288 28-29 20.882002967160375 26.874988167836182 30.967739403344957 21.275269461658482 30-31 21.019239600242603 26.46777765797872 30.894735031550375 21.618247710228296 32-33 20.85023995766716 26.50523885063198 30.909599668836023 21.734921522864838 34-35 21.44964181335323 26.53155410916699 30.75582242327598 21.262981654203802 36-37 21.308048386765407 26.304203752021866 30.76954329063338 21.618204570579344 38-39 21.026183854555324 26.716713456660724 30.670039023847544 21.5870636649364 40-41 21.284772950204836 26.552928278806764 30.71527320985517 21.44702556113323 42-43 20.961511427971935 26.533133928301446 30.449854705011585 22.055499938715037 44-45 21.121238086620266 26.71177792592237 30.41385894706129 21.753125040396075 46-47 21.557489902469023 26.930235156583294 29.80505347489884 21.70722146604884 48-49 21.359372135729426 27.039129774284515 29.688976866967632 21.912521223018427 50-51 21.54214857029933 27.286115126814398 29.501921458230502 21.669814844655768 52-53 21.619268415843024 27.359378185560278 29.24434094415995 21.777012454436747 54-55 21.431308715210655 27.382186358563835 29.256871529376134 21.929633396849376 56-57 21.53672562562588 27.56963521668277 28.982706966995092 21.910932190696254 58-59 21.684379726027146 27.115841054578127 29.16366885308998 22.036110366304744 60-61 21.501600305013426 27.31220302964003 29.47016841283161 21.71602825251493 62-63 21.340991031428423 27.360870368913076 29.69944219606424 21.598696403594257 64-65 21.31194607241949 27.365274519135124 29.42820110770515 21.89457830074024 66-67 21.362568216342563 27.387462363596164 29.092299717556763 22.15766970250451 68-69 21.328879763065014 27.301596606325912 29.444729209742064 21.924794420867006 70-71 21.61188908347173 27.230368647811314 29.186349331489463 21.971392937227492 72-73 21.36563047963552 27.330151350749993 29.070870779793463 22.233347389821027 74-75 21.201378681222902 27.76862678475141 28.964377913031917 22.065616620993772 76-77 21.414723067906365 27.415216589910685 29.052287709940174 22.117772632242776 78-79 21.387248946922202 27.310590838448206 29.07909676636042 22.223063448269176 80-81 21.52036522446029 27.455262704159463 28.887705573209114 22.13666649817113 82-83 21.457045866346764 27.371733727459684 29.05737164833547 22.113848757858083 84-85 21.385321746675913 27.392044346542754 28.899886802772585 22.32274710400875 86-87 21.420660276398742 27.565144521864237 28.83555126388692 22.178643937850094 88-89 21.524884760823458 27.431090192269565 28.690267606937407 22.35375743996957 90-91 21.47035349434508 27.163473189610325 28.906479721816908 22.45969359422769 92-93 21.512606903886205 27.00290154502888 28.99313248696474 22.491359064120175 94-95 21.32681383090929 26.972802633650026 29.074123566819594 22.62625996862109 96-97 21.257670510019384 26.968689223917263 29.071746533527275 22.70189373253607 98-99 21.128244283971302 27.19859775010352 28.83339419166821 22.839763774256973 100-101 21.44827409549792 26.409815406137188 29.494495137827514 22.647415360537376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2924.0 1 1717.5 2 501.5 3 501.0 4 435.0 5 421.5 6 702.5 7 1526.5 8 2585.0 9 3070.0 10 2827.0 11 2581.5 12 2711.5 13 2921.0 14 3150.5 15 3352.5 16 3523.0 17 3774.0 18 4044.5 19 4385.0 20 4858.5 21 5525.0 22 6479.0 23 7703.5 24 9413.0 25 11746.0 26 14766.0 27 18208.0 28 21264.5 29 24600.0 30 28803.5 31 33451.0 32 39652.0 33 45213.0 34 49610.5 35 54283.0 36 60519.5 37 65978.5 38 67894.0 39 68837.5 40 70141.0 41 72113.5 42 72543.5 43 75035.5 44 79998.0 45 81953.5 46 80951.5 47 81990.0 48 84536.0 49 82636.5 50 77450.0 51 72551.5 52 68662.5 53 65560.0 54 64189.0 55 58798.5 56 50938.5 57 45459.5 58 41319.0 59 36409.5 60 29363.5 61 23933.5 62 19043.0 63 13608.0 64 9696.0 65 7361.0 66 5263.0 67 3764.5 68 2870.5 69 2149.0 70 1605.0 71 1086.5 72 742.0 73 523.5 74 358.5 75 255.0 76 179.0 77 118.5 78 82.5 79 58.5 80 41.0 81 21.5 82 15.5 83 15.5 84 12.0 85 9.0 86 10.5 87 8.5 88 4.0 89 3.5 90 8.5 91 9.0 92 5.5 93 4.5 94 2.0 95 2.0 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35107554897020143 2 0.294728384089551 3 0.30039535161229836 4 0.294636238276173 5 0.2962487900102881 6 0.21737197375871525 7 0.20737415300720166 8 0.2146997451707531 9 0.21783270282560527 10-11 0.21762537474550478 12-13 0.22066618658697898 14-15 0.2159437136513562 16-17 0.20675216876690014 18-19 0.19951872241672666 20-21 0.19891977462976962 22-23 0.20769666335402467 24-25 0.20527783575285202 26-27 0.21239609983630298 28-29 0.2195834732797874 30-31 0.22158764472075904 32-33 0.22015938461339993 34-35 0.21988294717326592 36-37 0.20583071063312006 38-39 0.1950496504678934 40-41 0.18880677161153353 42-43 0.20099305543077478 44-45 0.20368832047208144 46-47 0.21179715204934593 48-49 0.22032063978681143 50-51 0.21516047423764312 52-53 0.22757712259032936 54-55 0.21267253727643695 56-57 0.1853282671565138 58-59 0.16659963058743416 60-61 0.1847984287295903 62-63 0.17744980011269434 64-65 0.18484450163627927 66-67 0.18521308488979132 68-69 0.18297854891537466 70-71 0.18498272035634628 72-73 0.19412819233411335 74-75 0.20018677956371722 76-77 0.2031584820451579 78-79 0.19915013916321467 80-81 0.19551037953478342 82-83 0.18399215286253273 84-85 0.17970737254045546 86-87 0.18436073611604475 88-89 0.19431248396086936 90-91 0.19028110462558162 92-93 0.18618061593026036 94-95 0.1865952720904614 96-97 0.19451981204096988 98-99 0.19534912436137195 100-101 0.17496186315148818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2170473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.38207840344188 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98009281666337 38.01250929399704 2 14.162623513272186 14.554100635147616 3 4.999045240913049 7.705840035328418 4 2.316326375125244 4.7607065365858245 5 1.248098085796304 3.2064936849785703 6 0.7509628358812096 2.315161878679162 7 0.486527393220459 1.7499152064713075 8 0.3270594190128569 1.3443994168242208 9 0.24014187559048758 1.1105089811586013 >10 1.3089173293029943 12.431235370448542 >50 0.11458277806666523 4.062812706608858 >100 0.06139964057258351 5.857048296272397 >500 0.0032344058929459516 1.083977517855914 >1k 7.187568650990824E-4 0.6492508756494726 >5k 1.796892162747706E-4 0.6378483609818517 >10k+ 8.98446081373853E-5 0.5181912030123245 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11225 0.5171683775840565 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8163 0.37609313730232996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5654 0.2604962144196219 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3090 0.14236528166901868 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2286 0.10532266469106043 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.214581337800562E-5 0.0 4 0.0 0.0 0.0 9.214581337800562E-5 0.0 5 4.607290668900281E-5 0.0 0.0 9.214581337800562E-5 0.0 6 4.607290668900281E-5 0.0 0.0 9.214581337800562E-5 0.0 7 9.214581337800562E-5 0.0 0.0 9.214581337800562E-5 0.0 8 1.3821872006700844E-4 0.0 0.0 9.214581337800562E-5 0.0 9 1.3821872006700844E-4 0.0 0.0 9.214581337800562E-5 4.607290668900281E-5 10-11 1.3821872006700844E-4 0.0 0.0 9.214581337800562E-5 4.607290668900281E-5 12-13 1.3821872006700844E-4 0.0 0.0 9.214581337800562E-5 6.910936003350421E-5 14-15 1.3821872006700844E-4 0.0 0.0 9.214581337800562E-5 9.214581337800562E-5 16-17 1.3821872006700844E-4 0.0 0.0 1.1518226672250703E-4 9.214581337800562E-5 18-19 1.3821872006700844E-4 0.0 0.0 1.3821872006700844E-4 9.214581337800562E-5 20-21 1.3821872006700844E-4 0.0 0.0 1.6125517341150983E-4 9.214581337800562E-5 22-23 1.3821872006700844E-4 0.0 0.0 2.3036453344501406E-4 1.3821872006700844E-4 24-25 2.3036453344501406E-4 0.0 0.0 4.146561602010253E-4 1.3821872006700844E-4 26-27 2.534009867895155E-4 0.0 0.0 6.910936003350421E-4 1.3821872006700844E-4 28-29 4.376926135455267E-4 0.0 0.0 0.0018429162675601125 1.3821872006700844E-4 30-31 5.06801973579031E-4 0.0 0.0 0.004031379335287746 1.3821872006700844E-4 32-33 5.06801973579031E-4 0.0 0.0 0.006588425656527402 1.8429162675601124E-4 34-35 5.298384269235324E-4 0.0 0.0 0.00868474291087703 1.8429162675601124E-4 36-37 5.528748802680338E-4 0.0 0.0 0.01211717445920774 1.8429162675601124E-4 38-39 6.910936003350421E-4 0.0 0.0 0.0179914700620556 1.8429162675601124E-4 40-41 6.910936003350421E-4 0.0 0.0 0.025270989318918042 1.8429162675601124E-4 42-43 7.37166507024045E-4 0.0 0.0 0.030707592308220375 2.0732808010051265E-4 44-45 8.753852270910534E-4 0.0 0.0 0.03374840414969456 2.3036453344501406E-4 46-47 9.675310404690591E-4 0.0 0.0 0.03812533028514983 2.3036453344501406E-4 48-49 0.0011057497605360676 0.0 0.0 0.0442299904214427 3.2251034682301966E-4 50-51 0.0011057497605360676 0.0 0.0 0.051624691945027654 3.2251034682301966E-4 52-53 0.0011057497605360676 0.0 0.0 0.0672664437659441 3.2251034682301966E-4 54-55 0.0011057497605360676 0.0 0.0 0.07742551969086922 3.2251034682301966E-4 56-57 0.0011518226672250704 0.0 0.0 0.10605983119808447 3.2251034682301966E-4 58-59 0.0011518226672250704 0.0 0.0 0.15515051327521695 3.2251034682301966E-4 60-61 0.0011518226672250704 0.0 0.0 0.19926532142993714 3.2251034682301966E-4 62-63 0.0011518226672250704 0.0 0.0 0.2229467954680846 3.2251034682301966E-4 64-65 0.0011518226672250704 0.0 0.0 0.2642050834080866 3.2251034682301966E-4 66-67 0.001243968480603076 0.0 0.0 0.34690595091484666 3.2251034682301966E-4 68-69 0.001243968480603076 0.0 0.0 0.46330914966461223 3.2251034682301966E-4 70-71 0.001243968480603076 0.0 0.0 0.5684014498222276 3.2251034682301966E-4 72-73 0.001243968480603076 0.0 0.0 0.6959773284440764 3.685832535120225E-4 74-75 0.001243968480603076 0.0 0.0 0.8511739146259825 3.685832535120225E-4 76-77 0.0012670049339475772 0.0 0.0 0.9704105971371217 3.685832535120225E-4 78-79 0.0012900413872920786 0.0 0.0 1.127265807959832 3.685832535120225E-4 80-81 0.0015664788274260956 0.0 0.0 1.2911701735059593 3.685832535120225E-4 82-83 0.0016586246408041012 0.0 0.0 1.4111900954308116 4.6072906689002813E-4 84-85 0.0016586246408041012 0.0 0.0 1.5925791290654159 4.6072906689002813E-4 86-87 0.0016586246408041012 0.0 0.0 1.8399215286253274 4.6072906689002813E-4 88-89 0.0016586246408041012 0.0 0.0 2.190835822422117 4.6072906689002813E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7270 0.0 36.70021 1 GTATCAA 11685 0.0 36.143013 1 ATCAACG 14085 0.0 29.834911 3 TCAACGC 14280 0.0 29.36364 4 CAACGCA 14380 0.0 29.091974 5 TATCAAC 14470 0.0 28.976067 2 AACGCAG 14725 0.0 28.42426 6 ACGCAGA 16695 0.0 24.90005 7 GTACATG 9715 0.0 24.8202 1 CGCAGAG 16745 0.0 24.768953 8 TACATGG 9830 0.0 23.986536 2 GAGTACT 9605 0.0 23.316462 12-13 ACATGGG 9535 0.0 23.182571 3 GCAGAGT 17985 0.0 22.557758 9 AGGACCG 655 0.0 22.499344 5 GACCGCG 110 0.0028749139 21.595133 7 GTACTTT 10470 0.0 21.388395 14-15 TAAGGTG 825 0.0 21.320492 5 AGAGTAC 14575 0.0 20.9228 10-11 CCTATAC 580 0.0 20.489973 3 >>END_MODULE