FastQCFastQC Report
Thu 26 May 2016
SRR937820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170473
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120370.5545795778155269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96840.4461700283763032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66010.30412725705410754No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34780.16024156946435178No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.11555084997601904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92550.032.3464781
GTATCAA136150.030.4690911
CCGTTGC2007.185008E-1026.1255519
TACATGG93550.024.8312072
GTACATG94900.024.7356681
ATCAACG166900.024.7341043
TCAACGC168250.024.5638794
TATCAAC170000.024.3669052
CAACGCA170200.024.2545385
AACGCAG174100.023.7630466
TATTCCG2801.8189894E-1223.7521445
ACATGGG93850.023.3851663
GGACCGA8400.022.6184836
GAGTACT110100.022.0031912-13
TAAGGTG10300.021.6767145
AGTACTT115650.020.82404512-13
ACGCAGA197150.020.7447957
GTACTTT116350.020.67739514-15
CGCAGAG197900.020.5461628
TCCGTTG2801.5770638E-920.3575738
Produced by FastQC (version 0.11.5)