##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937820_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2170473 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30257137499522 33.0 31.0 34.0 31.0 34.0 2 32.5348580701073 34.0 31.0 34.0 31.0 34.0 3 32.639813994461115 34.0 31.0 34.0 31.0 34.0 4 36.225961345752744 37.0 35.0 37.0 35.0 37.0 5 36.0864226369091 37.0 35.0 37.0 35.0 37.0 6 36.1771157715392 37.0 36.0 37.0 35.0 37.0 7 36.141855715321036 37.0 36.0 37.0 35.0 37.0 8 36.13543775941926 37.0 36.0 37.0 35.0 37.0 9 37.81674086708289 39.0 38.0 39.0 35.0 39.0 10-11 37.77504465616481 39.0 37.5 39.0 35.0 39.0 12-13 37.70225453161592 39.0 37.0 39.0 35.0 39.0 14-15 38.960367855301584 40.0 38.0 41.0 36.0 41.0 16-17 38.93924550086548 40.0 38.0 41.0 35.5 41.0 18-19 38.93328159345912 40.0 38.0 41.0 35.5 41.0 20-21 38.85564206511668 40.0 38.0 41.0 35.0 41.0 22-23 38.715813557689955 40.0 38.0 41.0 34.5 41.0 24-25 38.66886733905467 40.0 38.0 41.0 34.5 41.0 26-27 38.59187329213494 40.0 38.0 41.0 34.0 41.0 28-29 38.487819475294096 40.0 38.0 41.0 34.0 41.0 30-31 38.52982921234219 40.0 38.0 41.0 34.5 41.0 32-33 38.441301504326475 40.0 38.0 41.0 34.0 41.0 34-35 38.309890286587304 40.0 38.0 41.0 34.0 41.0 36-37 38.15514337197468 40.0 38.0 41.0 34.0 41.0 38-39 38.01269976636429 40.0 38.0 41.0 33.5 41.0 40-41 37.838109250840716 40.0 37.0 41.0 33.0 41.0 42-43 37.62717596579179 40.0 37.0 41.0 33.0 41.0 44-45 37.32504804252345 40.0 37.0 41.0 32.5 41.0 46-47 36.954063699479335 40.0 37.0 41.0 31.5 41.0 48-49 36.598630805359015 40.0 36.0 41.0 31.0 41.0 50-51 36.3400095278771 39.5 36.0 41.0 30.0 41.0 52-53 36.12610338852407 39.0 36.0 41.0 30.0 41.0 54-55 35.92633472058856 39.0 35.0 40.5 29.0 41.0 56-57 35.65596669481721 39.0 35.0 40.0 28.5 41.0 58-59 35.40637294267194 38.5 35.0 40.0 28.0 41.0 60-61 35.06568406978571 38.0 34.5 40.0 27.5 41.0 62-63 34.70954464764132 38.0 34.0 40.0 26.5 41.0 64-65 34.31395691169621 37.0 34.0 40.0 26.0 41.0 66-67 33.89771423095335 36.5 33.5 39.0 26.0 41.0 68-69 33.45738647750974 36.0 33.0 39.0 25.0 40.0 70-71 33.04789048285788 35.5 33.0 39.0 24.5 40.0 72-73 32.60552400329329 35.0 32.0 38.0 23.0 40.0 74-75 32.12623884286974 35.0 32.0 37.0 22.0 39.0 76-77 30.901136065733137 34.0 30.5 36.0 20.5 38.5 78-79 31.433764207156692 34.5 31.0 36.0 21.0 39.0 80-81 31.229233443585798 35.0 31.0 36.0 20.0 37.5 82-83 30.87952833322506 34.0 31.0 35.5 18.5 37.0 84-85 30.489614015009632 34.0 31.0 35.0 17.5 37.0 86-87 30.088420127778598 34.0 30.5 35.0 13.0 36.0 88-89 29.66273434408076 34.0 30.0 35.0 7.0 36.0 90-91 29.179546117367046 34.0 29.0 35.0 2.0 35.0 92-93 28.757711337574804 34.0 29.0 35.0 2.0 35.0 94-95 28.328326820927973 34.0 29.0 35.0 2.0 35.0 96-97 27.819369556774028 33.5 28.5 35.0 2.0 35.0 98-99 27.07087118798529 33.0 26.5 35.0 2.0 35.0 100-101 24.687319768548146 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16456624542295373 1101 2 0.10269481534180613 1101 3 0.13086256381004802 1101 4 0.12292395949547341 1101 5 0.027781220909012916 1101 6 0.03057862089580965 1101 7 0.07522778885142145 1101 8 0.12360686895733863 1101 9 0.24512440497537824 1101 10-11 0.19403099725169426 1101 12-13 0.22280253817221052 1101 14-15 0.19843159243158226 1101 16-17 0.040318401224361367 1101 18-19 0.21914877619455808 1101 20-21 0.27753618727344076 1101 22-23 0.14750424049903899 1101 24-25 0.15289705319608515 1101 26-27 0.14831875050460042 1101 28-29 0.1710135556853487 1101 30-31 0.14688967748323734 1101 32-33 0.14459824391286702 1101 34-35 0.025295317778500248 1101 36-37 0.051591847667289414 1101 38-39 0.09367096870788316 1101 40-41 0.02558679383808027 1101 42-43 0.04274165897771098 1101 44-45 -0.09050113806218008 1101 46-47 -0.261775927551561 1101 48-49 -0.1363872060021336 1101 50-51 -0.016379592401278842 1101 52-53 -0.028985693899166165 1101 54-55 0.04086242305671561 1101 56-57 0.06075154210342504 1101 58-59 -0.11147710572090119 1101 60-61 0.06416366967052767 1101 62-63 0.10364880382583408 1101 64-65 -0.1477239997457076 1101 66-67 -0.10896505031438153 1101 68-69 -0.2863766298961963 1101 70-71 -0.10766038573824233 1101 72-73 -0.16007648236031713 1101 74-75 -0.29227409961726636 1101 76-77 -0.3360572945917255 1101 78-79 -0.265934589328495 1101 80-81 -0.2691842086092002 1101 82-83 -0.2963734246980003 1101 84-85 -0.001572118595998262 1101 86-87 0.1417422851973349 1101 88-89 0.09623940257269581 1101 90-91 -0.019018543165348234 1101 92-93 0.11442753646269921 1101 94-95 0.27896187479828427 1101 96-97 0.39356196140205313 1101 98-99 0.6235793456491017 1101 100-101 1.3592149245060519 1102 1 0.24710372982508488 1102 2 0.2446854335125792 1102 3 0.20693724871659214 1102 4 0.11035801929885736 1102 5 -0.018728817846330514 1102 6 0.05497929960942116 1102 7 0.08284648120532978 1102 8 0.09028107321844203 1102 9 0.20619889181374873 1102 10-11 0.20458728822704586 1102 12-13 0.16832520707485088 1102 14-15 0.18309046951707586 1102 16-17 0.22653912839864887 1102 18-19 0.28909168304309674 1102 20-21 0.22434283312094294 1102 22-23 0.3119981267946983 1102 24-25 0.21257928582055285 1102 26-27 0.18215366398181487 1102 28-29 0.127174631327577 1102 30-31 0.19820724348945618 1102 32-33 0.2584546807984438 1102 34-35 0.35113768522137434 1102 36-37 0.2992368995941703 1102 38-39 0.32345514041978873 1102 40-41 0.39250148587507283 1102 42-43 0.4302684807097492 1102 44-45 0.372630781944693 1102 46-47 0.39385911202906243 1102 48-49 0.32436063340418997 1102 50-51 0.49709490302004156 1102 52-53 0.3599701071875714 1102 54-55 0.4182325563566067 1102 56-57 0.4417902281191459 1102 58-59 0.4530751281579981 1102 60-61 0.40852631929453054 1102 62-63 0.34194850408265864 1102 64-65 0.41362736989572113 1102 66-67 0.5757666993538564 1102 68-69 0.5876486270972592 1102 70-71 0.5270964525655017 1102 72-73 0.45605237419211164 1102 74-75 0.47348288393646953 1102 76-77 0.3875978224995791 1102 78-79 0.6080267906785011 1102 80-81 0.6223270020495093 1102 82-83 0.634827347107656 1102 84-85 0.7455349306503543 1102 86-87 0.8577376933168104 1102 88-89 0.8511391140533924 1102 90-91 0.8597641463363779 1102 92-93 1.1296860484425224 1102 94-95 0.9659092645898504 1102 96-97 1.1481200878091613 1102 98-99 1.3816431962264666 1102 100-101 1.9929269531863234 1103 1 0.11721265293692795 1103 2 0.16536397732655672 1103 3 0.10294777570441482 1103 4 0.05687308268068847 1103 5 -0.03363720325893382 1103 6 0.011604421088307504 1103 7 0.06196878295533281 1103 8 -0.018868148962006615 1103 9 0.13092578599765403 1103 10-11 0.08413458873566526 1103 12-13 0.08446469675712365 1103 14-15 0.14467591971605032 1103 16-17 0.03621381326291129 1103 18-19 0.025355081188813244 1103 20-21 0.08166118228495378 1103 22-23 0.0531556166330347 1103 24-25 0.1587380702513954 1103 26-27 0.10406464212626076 1103 28-29 0.13277560599613025 1103 30-31 -0.01976569066012246 1103 32-33 -0.010209707621868347 1103 34-35 0.10825572723001642 1103 36-37 -0.03602576081116382 1103 38-39 0.028847738897489705 1103 40-41 0.05937623316353324 1103 42-43 0.13307561834798065 1103 44-45 0.10537404119659044 1103 46-47 -0.04909830474459653 1103 48-49 0.04639034164205924 1103 50-51 0.0636771256048263 1103 52-53 -0.11478897446752256 1103 54-55 -0.08737967949332415 1103 56-57 0.04904127620705623 1103 58-59 0.041716231647370705 1103 60-61 -0.0667409342540708 1103 62-63 0.039739273106079054 1103 64-65 0.03089537182519564 1103 66-67 0.017595692551040543 1103 68-69 -0.08344001879444818 1103 70-71 -0.013879882992881676 1103 72-73 0.08566892653266223 1103 74-75 -0.08596161173994687 1103 76-77 -0.04943940501530264 1103 78-79 0.2089087983254636 1103 80-81 0.15710286020473063 1103 82-83 0.032280635372618605 1103 84-85 0.0896285740238163 1103 86-87 0.24867226496629868 1103 88-89 0.42567306075164524 1103 90-91 0.4612579819479592 1103 92-93 0.458617530804144 1103 94-95 0.614576987029217 1103 96-97 0.7641752516371838 1103 98-99 0.7816075505972258 1103 100-101 1.5818585419528084 1104 1 0.20365703295711057 1104 2 0.2387008492150855 1104 3 0.1632550794371852 1104 4 0.1170905747856068 1104 5 0.015607785484505143 1104 6 0.07059706840647806 1104 7 0.11085710598126042 1104 8 0.1009620692097073 1104 9 0.21142246838709156 1104 10-11 0.13127511210346654 1104 12-13 0.17719330993310933 1104 14-15 0.12362309344563016 1104 16-17 0.0836896677852792 1104 18-19 0.13889409806118636 1104 20-21 0.1826528093292623 1104 22-23 0.20548026674190112 1104 24-25 0.15413161121246333 1104 26-27 0.2102510631931125 1104 28-29 0.19879979835818062 1104 30-31 0.209612174558778 1104 32-33 0.2540031115255559 1104 34-35 0.15478098933114381 1104 36-37 0.20645221426352833 1104 38-39 0.020860869976871754 1104 40-41 0.10828872962569136 1104 42-43 0.17146148660877714 1104 44-45 0.07625330907697503 1104 46-47 -0.03802988187635492 1104 48-49 0.06890484355313475 1104 50-51 0.17250025777674693 1104 52-53 0.07955220476603131 1104 54-55 0.12322351226051609 1104 56-57 0.15015927612618896 1104 58-59 0.2898845992005974 1104 60-61 0.1027152838677452 1104 62-63 0.23296185023524885 1104 64-65 0.1720567118766212 1104 66-67 0.27830800887378615 1104 68-69 0.029501200183339904 1104 70-71 0.12970783346045778 1104 72-73 0.22847276985093856 1104 74-75 0.3011578179390142 1104 76-77 -0.028719845264330246 1104 78-79 0.09293170545215546 1104 80-81 0.22718922217140047 1104 82-83 0.2691945048171327 1104 84-85 0.07920259678469677 1104 86-87 0.17400525189973948 1104 88-89 0.23936141956458457 1104 90-91 0.28678521663589507 1104 92-93 0.6041507357286449 1104 94-95 0.7446250844249391 1104 96-97 0.8470189080027524 1104 98-99 1.0122554244961925 1104 100-101 1.9069958186818745 1105 1 0.16287062136271402 1105 2 0.15373940254330165 1105 3 0.08501407768650182 1105 4 0.053422264815999654 1105 5 -0.027961926308698537 1105 6 -0.0033659763526827646 1105 7 0.04945663884802798 1105 8 0.015415646254901105 1105 9 0.04097903093080646 1105 10-11 0.12358436956509422 1105 12-13 0.0862566272573062 1105 14-15 0.12033705276011375 1105 16-17 0.12845985264626592 1105 18-19 0.20062520396308514 1105 20-21 0.19822080858320845 1105 22-23 0.22110845920757782 1105 24-25 0.08851168471600346 1105 26-27 -0.0026120565956233577 1105 28-29 0.08135775551926372 1105 30-31 0.04490277636372042 1105 32-33 0.13087026680285163 1105 34-35 0.011134976283628362 1105 36-37 0.1139267699146913 1105 38-39 0.08321255941380912 1105 40-41 0.16950522595686834 1105 42-43 0.08293634746496537 1105 44-45 0.06694201267627875 1105 46-47 0.14268116103462347 1105 48-49 0.07614328921180658 1105 50-51 0.013264202890709953 1105 52-53 0.06593370255965425 1105 54-55 -0.03868266263042841 1105 56-57 0.023015104906178863 1105 58-59 0.03607610687100049 1105 60-61 -0.007133088584112102 1105 62-63 -0.07181333659684697 1105 64-65 -0.008122341733482585 1105 66-67 0.07443232905080777 1105 68-69 0.01990377407898336 1105 70-71 0.06654819851956972 1105 72-73 -0.011512902469078767 1105 74-75 0.17136641709036837 1105 76-77 0.0020609285151671486 1105 78-79 0.18938624050198527 1105 80-81 0.13562220635584765 1105 82-83 0.1867467605789379 1105 84-85 0.21296039116976928 1105 86-87 0.36027229781189973 1105 88-89 0.4388518967441506 1105 90-91 0.33721398729551666 1105 92-93 0.5783770198151679 1105 94-95 0.6415187755828917 1105 96-97 0.5816842634182358 1105 98-99 0.9080168898193932 1105 100-101 1.4817320494846946 1106 1 0.030870869086747632 1106 2 0.1526370305856659 1106 3 0.048692425721071686 1106 4 0.1302981108378205 1106 5 0.0989594832410603 1106 6 0.04423950542632582 1106 7 0.04618668158042283 1106 8 0.13356408603583958 1106 9 0.1940954877693173 1106 10-11 0.14719335486974217 1106 12-13 -0.01276840171755822 1106 14-15 0.22336724855056644 1106 16-17 0.15100539405599278 1106 18-19 0.3205315996668432 1106 20-21 0.2679931678892231 1106 22-23 0.3158036259346986 1106 24-25 0.1915471888786513 1106 26-27 0.030158294376249728 1106 28-29 0.18727039740763018 1106 30-31 0.08333981464593165 1106 32-33 0.12444448226967353 1106 34-35 -0.07285497319836054 1106 36-37 0.16304779195328223 1106 38-39 0.04283088745351904 1106 40-41 0.014281345359854924 1106 42-43 0.034899118081355596 1106 44-45 0.10962486397992421 1106 46-47 0.023601986663443597 1106 48-49 0.09367418836158237 1106 50-51 -0.10048094216055858 1106 52-53 0.08689381014266218 1106 54-55 -0.10173462274686074 1106 56-57 -0.25435884337258585 1106 58-59 -0.1752006451130086 1106 60-61 -0.014785991821327116 1106 62-63 0.07488175935197461 1106 64-65 -0.04372647872487789 1106 66-67 -0.08176121419916171 1106 68-69 -0.34340210317375863 1106 70-71 -0.05099420210328276 1106 72-73 0.28390154868947093 1106 74-75 0.16202545149747039 1106 76-77 0.04762799537411766 1106 78-79 0.2165270627688365 1106 80-81 0.18250840370343013 1106 82-83 0.2530660237768778 1106 84-85 0.34023038382652615 1106 86-87 0.3897319939960653 1106 88-89 0.39529670294433217 1106 90-91 0.39994302143719906 1106 92-93 0.7287862068379667 1106 94-95 0.616435832267463 1106 96-97 0.8137309063151932 1106 98-99 1.0376614943014282 1106 100-101 1.9759813129480541 1107 1 0.17449223678789139 1107 2 0.14131420136028083 1107 3 0.1857844195256888 1107 4 0.13031659500835957 1107 5 -0.0668779452921271 1107 6 0.008372565958801204 1107 7 0.03675975460564729 1107 8 0.08425692373845095 1107 9 0.20577142839051277 1107 10-11 0.1311318854419241 1107 12-13 0.19425064335194975 1107 14-15 0.2501339209537079 1107 16-17 0.22648183948626155 1107 18-19 0.13703589245609749 1107 20-21 0.16112231487662854 1107 22-23 0.25483040248656863 1107 24-25 0.2447844682637026 1107 26-27 0.16429935409286855 1107 28-29 0.17862888833445822 1107 30-31 0.29274937287426184 1107 32-33 0.22455506860031704 1107 34-35 0.2636861604549381 1107 36-37 0.2835022794637254 1107 38-39 0.2681272715673302 1107 40-41 0.41190039297890735 1107 42-43 0.44779007422287975 1107 44-45 0.4862567294151603 1107 46-47 0.5354288282286035 1107 48-49 0.6110872330868986 1107 50-51 0.7128383368293143 1107 52-53 0.6345801018993598 1107 54-55 0.6547894927218394 1107 56-57 0.759851659460594 1107 58-59 0.6123118635713638 1107 60-61 0.721028522714235 1107 62-63 0.5858648622705687 1107 64-65 0.6625278634617402 1107 66-67 0.7766304398791561 1107 68-69 0.8771801800674695 1107 70-71 0.952836801587182 1107 72-73 0.9510550182957616 1107 74-75 0.9686662084561277 1107 76-77 0.9157883742358699 1107 78-79 0.9184511693141495 1107 80-81 0.9324164608896268 1107 82-83 0.961627541008589 1107 84-85 0.9481261522262407 1107 86-87 1.016522727690159 1107 88-89 1.1201722534857872 1107 90-91 1.12119328435859 1107 92-93 1.2836169492004998 1107 94-95 1.2247604015544162 1107 96-97 1.5640970841074164 1107 98-99 1.7922592723766542 1107 100-101 2.4619072105457462 1108 1 0.14648289813537474 1108 2 0.21855935904029877 1108 3 0.08880263350839357 1108 4 0.09947316786729488 1108 5 -0.07635860308948139 1108 6 0.054188524705061525 1108 7 0.10154665642343019 1108 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-0.07780478220959708 2316 76-77 0.02252617627465625 2316 78-79 -0.13830830286569196 2316 80-81 -0.0693456260345009 2316 82-83 -0.1531581215962703 2316 84-85 -0.2509212570278052 2316 86-87 -0.03540663123185084 2316 88-89 -0.19272918139991546 2316 90-91 -0.3243091763665156 2316 92-93 -0.42610954138283574 2316 94-95 -0.3430900637267911 2316 96-97 -0.706834123044807 2316 98-99 -0.9648402956532607 2316 100-101 -1.1918949142026314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 15.0 5 31.0 6 85.0 7 189.0 8 433.0 9 820.0 10 1512.0 11 2532.0 12 4158.0 13 6016.0 14 7927.0 15 11283.0 16 15644.0 17 23134.0 18 21281.0 19 12440.0 20 10077.0 21 10136.0 22 10249.0 23 11076.0 24 12285.0 25 14177.0 26 17509.0 27 21459.0 28 27055.0 29 34195.0 30 43387.0 31 54734.0 32 71344.0 33 96043.0 34 135479.0 35 200555.0 36 318199.0 37 508409.0 38 426710.0 39 39885.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7598118253549 19.996321689036144 15.405715855548397 21.838150630060557 2 15.45709161090693 20.979712716997632 40.365993956156096 23.197201715939336 3 19.427193980298306 25.063799457537595 30.20369292776275 25.30531363440135 4 12.89009354182245 17.304200512975743 37.34149192364982 32.46421402155198 5 13.11415580663311 38.385143999413856 34.24287122375655 14.257828970196481 6 31.46668951882838 37.22962690620892 17.11074037778862 14.19294319717407 7 28.216292024825922 33.62497483267472 20.53400341768822 17.624729724811136 8 26.340018972822975 35.548795124380725 19.409455911223038 18.701729991573266 9 26.45925565533411 17.286646735527235 20.446510967885803 35.807586641252854 10-11 24.629147655833545 27.63768542617208 26.8497696124301 20.883397305564273 12-13 25.75245134571467 24.81515414629494 27.800033127694363 21.632361380296025 14-15 22.635711202120458 26.124582061145198 26.667297865488305 24.572408871246036 16-17 21.36273982675666 29.50677110473155 27.36412754270613 21.766361525805667 18-19 21.24730415904736 27.835591596854698 30.33172953545149 20.585374708646455 20-21 21.918744825431897 26.69033587640655 30.743442321966064 20.647476976195488 22-23 21.747800183877604 26.464138683614184 30.718502551641574 21.06955858086664 24-25 21.71059012104108 26.668337586269097 30.71849005384339 20.90258223884644 26-27 21.203079574093614 27.032592619895595 30.825758029514887 20.938569776495903 28-29 21.145622376514023 27.29323860813018 30.65514784360712 20.905991171748674 30-31 21.288961940227164 26.9695584015886 30.576408077983196 21.16507158020104 32-33 21.256495703931815 26.964606332352442 30.53977174560568 21.23912621811006 34-35 21.72871996104075 26.903928314243025 30.54276648454047 20.824585240175757 36-37 21.798681669848 26.733573741760434 30.426063811897226 21.04168077649434 38-39 21.366218331211677 27.212432497432587 30.385542690464245 21.03580648089149 40-41 21.72079542109024 26.94177720708804 30.31790766344479 21.01951970837693 42-43 21.410678522117347 27.110726926600968 30.03268972956385 21.445904821717832 44-45 21.539682824895774 27.292253808271283 29.93909622464781 21.228967142185137 46-47 22.05894751973418 27.58398745342605 29.260580527838865 21.09648449900091 48-49 21.869680019055753 27.71018114484723 29.040628471305563 21.379510364791454 50-51 22.03111948409402 28.03464959020453 28.8670257589014 21.067205166800047 52-53 22.223865489227464 28.05123583661257 28.540000267222858 21.184898406937105 54-55 21.89902846061665 28.27236275226644 28.461169523877977 21.367439263238936 56-57 22.08343526963938 28.37950529677172 28.305235771189047 21.231823662399854 58-59 22.101242448074682 27.840060668803527 28.44978951592579 21.608907367195997 60-61 21.896816961095578 28.16775421762906 28.73832109406567 21.19710772720969 62-63 21.759750063695794 28.18519281281085 29.00377014595436 21.051286977538997 64-65 21.82984999122311 28.215877368665726 28.71947727522987 21.234795364881297 66-67 21.796493206780273 28.19903772127089 28.460570576091015 21.543898495857817 68-69 21.82738509071525 28.15298785103523 28.67156145227331 21.348065605976206 70-71 22.065950601550906 28.156857975197113 28.33628891029743 21.440902512954548 72-73 21.912640159670413 28.147623257809016 28.32405961655327 21.615676965967296 74-75 21.737954572993605 28.707340480476358 28.028317356002454 21.526387590527584 76-77 21.994445450369575 28.233730620007712 28.27307688232012 21.498747047302594 78-79 21.956181901364356 28.265221451729644 28.1472748106058 21.6313218363002 80-81 22.036371795456567 28.523552239534883 28.031355377376265 21.40872058763228 82-83 21.992965575642867 28.435375027240656 28.175154770697024 21.396504626419453 84-85 21.810242176048327 28.652918623715223 27.952401016094925 21.58443818414152 86-87 21.810430261053696 28.734474006357143 27.9155741628668 21.53952156972236 88-89 21.94468671114545 28.565547693981912 27.737364159793742 21.752401435078898 90-91 21.935925912017527 28.42505210722373 27.828217913365737 21.810804067393008 92-93 21.902083879987938 28.322310395994098 27.935860626516284 21.83974509750168 94-95 21.766660999699145 28.289041144487857 28.015782734915383 21.92851512089761 96-97 21.795871222539972 28.423942615273262 27.96825853166568 21.81192763052109 98-99 21.443661996165343 28.6705645998222 27.669374302713262 22.216399101299192 100-101 21.377370558169797 28.80568012371415 27.920332288251025 21.896617029865023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 680.0 1 482.0 2 322.5 3 403.0 4 383.0 5 380.5 6 651.5 7 1438.0 8 2419.0 9 2989.5 10 2931.5 11 2807.5 12 3065.0 13 3349.5 14 3609.5 15 3821.0 16 3943.0 17 4133.5 18 4410.5 19 4759.0 20 5172.0 21 5814.0 22 6734.0 23 7961.5 24 9668.0 25 11936.0 26 14855.0 27 18323.0 28 21487.5 29 25043.0 30 29167.5 31 33284.0 32 39094.0 33 44663.5 34 49272.0 35 53850.0 36 60246.5 37 65891.5 38 68284.0 39 69610.0 40 70420.0 41 72295.0 42 72662.5 43 74910.5 44 80280.5 45 82094.0 46 80055.0 47 80826.0 48 84239.5 49 82173.0 50 77351.0 51 73109.5 52 68461.5 53 65833.0 54 65729.0 55 60705.5 56 51286.0 57 44996.0 58 41136.5 59 36262.0 60 28941.0 61 23070.0 62 18677.5 63 13376.5 64 9100.5 65 6599.5 66 4842.5 67 3784.0 68 2974.5 69 2302.0 70 1700.5 71 1016.5 72 705.0 73 550.5 74 354.0 75 231.0 76 167.0 77 108.0 78 65.0 79 35.0 80 18.5 81 11.0 82 7.5 83 4.0 84 3.0 85 3.0 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04602683378231381 2 0.0 3 0.0 4 0.0 5 0.016586246408041013 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003847087708531735 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.214581337800562E-5 22-23 3.4554680016752107E-4 24-25 5.298384269235323E-4 26-27 0.001981134987627121 28-29 0.002510973414550653 30-31 2.534009867895155E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010113003018236116 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.214581337800562E-5 74-75 9.214581337800562E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8429162675601124E-4 84-85 2.994738934785183E-4 86-87 0.0 88-89 0.0 90-91 0.00868474291087703 92-93 0.0034324315483307095 94-95 0.0 96-97 0.0 98-99 2.994738934785183E-4 100-101 0.014236528166901869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2170473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.91162819676477 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17692594765272 35.30290966337014 2 14.862073970265136 14.538564725328479 3 5.3806173482282835 7.895242656168127 4 2.4889436447499897 4.869531446188483 5 1.4266083812905581 3.48888693640361 6 0.8286624519614282 2.431873785057409 7 0.5552881471362743 1.90120331763599 8 0.3699716457342117 1.4476732463597548 9 0.2770126187056371 1.219422439074806 >10 1.4427710545595458 13.095337300429682 >50 0.11882109250891473 4.038180811759002 >100 0.06642307096324662 6.173188159548613 >500 0.004742440519464776 1.558635299259537 >1k 8.536392935036556E-4 0.7254332283990025 >5k 1.8969762077859016E-4 0.7554953075703623 >10k+ 9.484881038929508E-5 0.5584216774470034 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12037 0.5545795778155269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9684 0.4461700283763032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6601 0.30412725705410754 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3478 0.16024156946435178 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.11555084997601904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.607290668900281E-5 0.0 9 0.0 0.0 0.0 4.607290668900281E-5 0.0 10-11 0.0 0.0 0.0 4.607290668900281E-5 0.0 12-13 1.1518226672250703E-4 0.0 0.0 4.607290668900281E-5 0.0 14-15 1.8429162675601124E-4 0.0 0.0 4.607290668900281E-5 0.0 16-17 2.3036453344501406E-4 0.0 0.0 6.910936003350421E-5 0.0 18-19 2.534009867895155E-4 0.0 0.0 9.214581337800562E-5 0.0 20-21 2.764374401340169E-4 0.0 0.0 1.1518226672250703E-4 0.0 22-23 2.764374401340169E-4 0.0 0.0 1.8429162675601124E-4 0.0 24-25 3.685832535120225E-4 0.0 0.0 3.685832535120225E-4 0.0 26-27 3.685832535120225E-4 0.0 0.0 6.910936003350421E-4 0.0 28-29 3.916197068565239E-4 0.0 0.0 0.001819879814215611 0.0 30-31 4.146561602010253E-4 0.0 0.0 0.003962269975254242 0.0 32-33 4.376926135455267E-4 0.0 0.0 0.006450206936460394 9.214581337800562E-5 34-35 4.6072906689002813E-4 0.0 0.0 0.00852348773746552 9.214581337800562E-5 36-37 4.6072906689002813E-4 0.0 0.0 0.011932882832451728 9.214581337800562E-5 38-39 4.6072906689002813E-4 0.0 0.0 0.01785325134198859 9.214581337800562E-5 40-41 4.6072906689002813E-4 0.0 0.0 0.025109734145506532 9.214581337800562E-5 42-43 4.8376552023452954E-4 0.0 0.0 0.030707592308220375 9.214581337800562E-5 44-45 6.450206936460393E-4 0.0 0.0 0.03377144060303906 9.214581337800562E-5 46-47 6.910936003350421E-4 0.0 0.0 0.038079257378460823 1.1518226672250703E-4 48-49 6.910936003350421E-4 0.0 0.0 0.04413784460806469 1.3821872006700844E-4 50-51 6.910936003350421E-4 0.0 0.0 0.05134825450489364 1.3821872006700844E-4 52-53 6.910936003350421E-4 0.0 0.0 0.0673125166726331 1.3821872006700844E-4 54-55 6.910936003350421E-4 0.0 0.0 0.07772499358434774 1.3821872006700844E-4 56-57 6.910936003350421E-4 0.0 0.0 0.106543596718319 1.3821872006700844E-4 58-59 6.910936003350421E-4 0.0 0.0 0.15637144530247554 1.3821872006700844E-4 60-61 7.141300536795436E-4 0.0 0.0 0.20092394607074127 1.3821872006700844E-4 62-63 7.37166507024045E-4 0.0 0.0 0.22488185754902273 1.8429162675601124E-4 64-65 7.37166507024045E-4 0.0 0.0 0.2668773119960488 1.8429162675601124E-4 66-67 8.293123204020506E-4 0.0 0.0 0.3506378563566559 2.0732808010051265E-4 68-69 8.293123204020506E-4 0.0 0.0 0.4685384245738141 2.3036453344501406E-4 70-71 8.293123204020506E-4 0.0 0.0 0.5743678912384536 2.3036453344501406E-4 72-73 8.293123204020506E-4 0.0 0.0 0.7032568477009389 2.3036453344501406E-4 74-75 8.52348773746552E-4 0.0 0.0 0.8602963501504051 2.3036453344501406E-4 76-77 8.753852270910534E-4 0.0 0.0 0.9810534385822814 2.534009867895155E-4 78-79 8.753852270910534E-4 0.0 0.0 1.1403965863661978 2.764374401340169E-4 80-81 0.001013603947158062 0.0 0.0 1.307318727300455 2.764374401340169E-4 82-83 0.001013603947158062 0.0 0.0 1.4289051280527332 2.764374401340169E-4 84-85 0.001013603947158062 0.0 0.0 1.611883676968108 2.764374401340169E-4 86-87 0.001013603947158062 0.0 0.0 1.8618983051159816 2.764374401340169E-4 88-89 0.001013603947158062 0.0 0.0 2.219447097475988 2.764374401340169E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9255 0.0 32.346478 1 GTATCAA 13615 0.0 30.469091 1 CCGTTGC 200 7.185008E-10 26.125551 9 TACATGG 9355 0.0 24.831207 2 GTACATG 9490 0.0 24.735668 1 ATCAACG 16690 0.0 24.734104 3 TCAACGC 16825 0.0 24.563879 4 TATCAAC 17000 0.0 24.366905 2 CAACGCA 17020 0.0 24.254538 5 AACGCAG 17410 0.0 23.763046 6 TATTCCG 280 1.8189894E-12 23.752144 5 ACATGGG 9385 0.0 23.385166 3 GGACCGA 840 0.0 22.618483 6 GAGTACT 11010 0.0 22.00319 12-13 TAAGGTG 1030 0.0 21.676714 5 AGTACTT 11565 0.0 20.824045 12-13 ACGCAGA 19715 0.0 20.744795 7 GTACTTT 11635 0.0 20.677395 14-15 CGCAGAG 19790 0.0 20.546162 8 TCCGTTG 280 1.5770638E-9 20.357573 8 >>END_MODULE