FastQCFastQC Report
Thu 26 May 2016
SRR937819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621905
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98970.6102083660880262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76170.469632931645195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.31370518001979153No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22060.13601289841266906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.11844096910731516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.043.5867161
GTATCAA91450.038.011221
TCAACGC113600.030.1773664
ATCAACG113950.030.0818843
CAACGCA115850.029.591275
TATCAAC115700.029.5857962
AACGCAG118650.028.9435636
GTACATG75300.026.3341431
ACGCAGA132150.025.8446127
TACATGG76350.025.4059372
CGCAGAG133250.025.345298
GAGTACT75800.024.1581412-13
ACATGGG77650.024.0009633
GCAGAGT138400.023.9567369
GTACTTT82400.022.16481214-15
AGTACTT77700.021.58052412-13
ACCGTGC2701.0022632E-921.1111728
GGACCGC2257.158269E-821.1105216
AGAGTAC121700.020.86245510-11
CATGGGA50300.020.7014394
Produced by FastQC (version 0.11.5)