##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937819_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1621905 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780611688107502 33.0 31.0 34.0 30.0 34.0 2 31.910965192166003 33.0 31.0 34.0 30.0 34.0 3 31.926143023173367 34.0 31.0 34.0 30.0 34.0 4 35.51324830985785 37.0 35.0 37.0 33.0 37.0 5 35.505678815960245 37.0 35.0 37.0 33.0 37.0 6 35.57334985711247 37.0 35.0 37.0 33.0 37.0 7 35.52076724592377 37.0 35.0 37.0 33.0 37.0 8 35.50977769968031 37.0 35.0 37.0 33.0 37.0 9 37.15024801082677 39.0 37.0 39.0 34.0 39.0 10-11 37.02866444088895 39.0 37.0 39.0 33.0 39.0 12-13 36.916184055169694 39.0 37.0 39.0 33.0 39.0 14-15 38.07380796039226 40.0 38.0 41.0 33.0 41.0 16-17 37.99685308325704 40.0 38.0 41.0 33.0 41.0 18-19 37.955408917291706 40.0 38.0 41.0 33.0 41.0 20-21 37.87381135146633 40.0 38.0 41.0 33.0 41.0 22-23 37.78870587364858 40.0 38.0 41.0 33.0 41.0 24-25 37.82083075149284 40.0 38.0 41.0 33.0 41.0 26-27 37.733541421969846 40.0 37.5 41.0 33.0 41.0 28-29 37.60734599128802 40.0 37.0 41.0 32.0 41.0 30-31 37.3535345781658 40.0 37.0 41.0 32.0 41.0 32-33 37.19351749948363 39.5 36.0 41.0 32.0 41.0 34-35 37.18280694615282 40.0 36.0 41.0 31.5 41.0 36-37 37.04274510529285 40.0 36.0 41.0 31.0 41.0 38-39 36.897842043769515 40.0 36.0 41.0 31.0 41.0 40-41 36.657235473101075 39.5 36.0 41.0 30.5 41.0 42-43 36.374035778914305 39.0 35.0 41.0 30.0 41.0 44-45 36.087677761644485 39.0 35.0 41.0 29.5 41.0 46-47 35.6742127313252 39.0 35.0 41.0 27.5 41.0 48-49 35.31190729420034 39.0 35.0 40.0 26.0 41.0 50-51 34.409985788316824 38.0 34.0 39.5 24.5 40.5 52-53 34.49533696486539 38.0 34.5 40.0 24.0 40.5 54-55 34.78526146722527 39.0 35.0 40.0 24.0 41.0 56-57 34.568581698681484 38.0 34.0 40.0 23.5 41.0 58-59 34.32307841704662 38.0 34.0 40.0 23.0 41.0 60-61 34.00710430019021 38.0 34.0 40.0 22.0 41.0 62-63 33.639853752223466 37.5 33.0 40.0 21.0 41.0 64-65 33.24383086555625 37.0 33.0 40.0 19.5 41.0 66-67 32.80742768534532 36.0 32.0 39.0 18.0 41.0 68-69 32.315813503256976 36.0 32.0 39.0 13.5 40.0 70-71 31.8701573150092 35.0 31.0 38.5 10.0 40.0 72-73 31.413986639168137 35.0 31.0 38.0 7.5 40.0 74-75 30.883083164550328 35.0 30.5 37.0 6.0 39.0 76-77 30.40740672234194 34.5 30.0 36.5 2.0 39.0 78-79 29.855726753416505 34.0 29.5 36.0 2.0 38.5 80-81 29.30300819098529 34.0 29.0 35.5 2.0 37.5 82-83 28.81396074369337 34.0 29.0 35.0 2.0 37.0 84-85 28.299655343562044 34.0 27.5 35.0 2.0 36.5 86-87 27.782703980812684 33.5 26.5 35.0 2.0 36.0 88-89 27.324470915374206 33.0 26.0 35.0 2.0 36.0 90-91 26.78693110878874 33.0 25.0 35.0 2.0 35.0 92-93 26.181656138923056 32.0 24.5 35.0 2.0 35.0 94-95 25.459446453398932 32.0 21.5 34.0 2.0 35.0 96-97 24.652862220660275 31.5 16.5 34.0 2.0 35.0 98-99 23.63944805645213 31.0 2.0 34.0 2.0 35.0 100-101 20.859326224408953 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2201025364301863 1101 2 0.31767389790441314 1101 3 0.28940508137174703 1101 4 0.15153520148010813 1101 5 0.17605942165158694 1101 6 0.23312239984444005 1101 7 0.2645046618308555 1101 8 0.3276237130727324 1101 9 0.3799381988271051 1101 10-11 0.3429496713054405 1101 12-13 0.2394975786303064 1101 14-15 0.4710883127642447 1101 16-17 0.476865163826929 1101 18-19 0.48654960004527226 1101 20-21 0.46994821825882127 1101 22-23 0.6040482463645134 1101 24-25 0.5597421225486343 1101 26-27 0.4518275457933285 1101 28-29 0.5492774668813141 1101 30-31 0.5476817946896091 1101 32-33 0.5912780114089244 1101 34-35 0.661808330455365 1101 36-37 0.7322684529927344 1101 38-39 0.5581403822726827 1101 40-41 0.6297770299311551 1101 42-43 0.7268298818003629 1101 44-45 0.8608855550239625 1101 46-47 0.7890682572438266 1101 48-49 0.7911111676251963 1101 50-51 0.7592617312413452 1101 52-53 0.6494224344961168 1101 54-55 0.7664748716920329 1101 56-57 0.7579156498109043 1101 58-59 0.8171687435345731 1101 60-61 0.9573648832905732 1101 62-63 1.0204246885363162 1101 64-65 0.8179711405949064 1101 66-67 0.965410383519334 1101 68-69 0.7856643350892014 1101 70-71 0.9001309490648701 1101 72-73 1.0710809380424955 1101 74-75 0.9648319602203479 1101 76-77 0.8079376093924822 1101 78-79 0.9003374100121846 1101 80-81 0.8399805095827411 1101 82-83 0.9234191929222071 1101 84-85 1.0349164216259048 1101 86-87 1.0826801475608399 1101 88-89 0.826162341859753 1101 90-91 1.0035941846564533 1101 92-93 1.1373031479444649 1101 94-95 1.2753387617187677 1101 96-97 1.3848294523395523 1101 98-99 1.3881467738776685 1101 100-101 1.7165392699669404 1102 1 0.23589731958976046 1102 2 0.33232847273926325 1102 3 0.3647994201542062 1102 4 0.23483640010474716 1102 5 0.1590354858842815 1102 6 0.20407950918967543 1102 7 0.2865198064599994 1102 8 0.31426944627201436 1102 9 0.37723658796006987 1102 10-11 0.34702877089090123 1102 12-13 0.3742160326753847 1102 14-15 0.48758518192548195 1102 16-17 0.5652356127488147 1102 18-19 0.4897340922939222 1102 20-21 0.4671691397904496 1102 22-23 0.5239740037107339 1102 24-25 0.5837298218412101 1102 26-27 0.4783165878576696 1102 28-29 0.5489254196708444 1102 30-31 0.4919071301530167 1102 32-33 0.6277113112303425 1102 34-35 0.6969611195185408 1102 36-37 0.8088030000999211 1102 38-39 0.7442270289240227 1102 40-41 0.6649126039718212 1102 42-43 0.7771212747481755 1102 44-45 0.7106296824059442 1102 46-47 0.9149132279323808 1102 48-49 0.9469863105098781 1102 50-51 1.0114821822290239 1102 52-53 1.006801424364511 1102 54-55 0.889701724372749 1102 56-57 0.8954906499256765 1102 58-59 0.7858959336297389 1102 60-61 0.9361106426566934 1102 62-63 0.9446671127787383 1102 64-65 0.892006640266672 1102 66-67 0.9818082663010941 1102 68-69 0.8667938586232893 1102 70-71 0.7068495706694868 1102 72-73 0.8807183667149125 1102 74-75 0.8826460081377903 1102 76-77 0.8802771424292573 1102 78-79 1.0182006355106168 1102 80-81 1.105194859566442 1102 82-83 1.1650463798039006 1102 84-85 1.1021680547577084 1102 86-87 1.2106764222299695 1102 88-89 1.287366854031923 1102 90-91 1.3847169943317539 1102 92-93 1.4737687546248104 1102 94-95 1.639571338968313 1102 96-97 1.704041589619024 1102 98-99 2.03541670084007 1102 100-101 2.194050138384494 1103 1 0.4029145305302677 1103 2 0.36716425058035895 1103 3 0.3579379566220737 1103 4 0.3288415055177154 1103 5 0.3746249143306528 1103 6 0.30165017862152865 1103 7 0.398702297067274 1103 8 0.41746194501622824 1103 9 0.5015316561546328 1103 10-11 0.4855680294016338 1103 12-13 0.5173384127730785 1103 14-15 0.5976102096116236 1103 16-17 0.4671237049164958 1103 18-19 0.5153766017600816 1103 20-21 0.4404669193176858 1103 22-23 0.47429698423790967 1103 24-25 0.4968269098758 1103 26-27 0.483002522709306 1103 28-29 0.46352225948484005 1103 30-31 0.43766887363098306 1103 32-33 0.36046203606385774 1103 34-35 0.5077432934609689 1103 36-37 0.3694415292239839 1103 38-39 0.4898978561007894 1103 40-41 0.7514503819715443 1103 42-43 0.6742561628139043 1103 44-45 0.4227014284395594 1103 46-47 0.5091910822489254 1103 48-49 0.5727314863646242 1103 50-51 0.2788845022837805 1103 52-53 0.34446221696665447 1103 54-55 0.42452340210898143 1103 56-57 0.5250383009363304 1103 58-59 0.4629722475098319 1103 60-61 0.5828157712760955 1103 62-63 0.5233374728895441 1103 64-65 0.5613064525590303 1103 66-67 0.5438061336602331 1103 68-69 0.5236080280536726 1103 70-71 0.6042238667658175 1103 72-73 0.4543878896319953 1103 74-75 0.4452316736186752 1103 76-77 0.5251326604171709 1103 78-79 0.6446737292792477 1103 80-81 0.6942616970010462 1103 82-83 0.7668660277125312 1103 84-85 0.7037460914943559 1103 86-87 0.6997417909036479 1103 88-89 0.8155009695294062 1103 90-91 0.8054705861865941 1103 92-93 0.7416086185587965 1103 94-95 0.9941894170652681 1103 96-97 1.2432058059028819 1103 98-99 1.1160838414269278 1103 100-101 1.4416423986019282 1104 1 0.30711390276767503 1104 2 0.3530136275037066 1104 3 0.25893368089202795 1104 4 0.31309674032950596 1104 5 0.29571119250967826 1104 6 0.23714887808057483 1104 7 0.2937479504335485 1104 8 0.44395580028668036 1104 9 0.2975358535295882 1104 10-11 0.3526966838602661 1104 12-13 0.511397406478558 1104 14-15 0.5666879086758243 1104 16-17 0.5918124738657511 1104 18-19 0.4143633628216037 1104 20-21 0.5913593224070368 1104 22-23 0.5408261453928276 1104 24-25 0.46607023010385973 1104 26-27 0.5331156766862861 1104 28-29 0.5611637710929145 1104 30-31 0.5119751621651787 1104 32-33 0.5667706967601731 1104 34-35 0.450159365166634 1104 36-37 0.6493366870326795 1104 38-39 0.7966511435579875 1104 40-41 0.7867236953012835 1104 42-43 0.8244926876578447 1104 44-45 0.6852092831538457 1104 46-47 0.7643804173521644 1104 48-49 0.9670661056433616 1104 50-51 0.8931840000429787 1104 52-53 0.7903388184449511 1104 54-55 1.0078231656609589 1104 56-57 1.0045077293896796 1104 58-59 0.9005038776184691 1104 60-61 0.9971209536206871 1104 62-63 1.0572572403439082 1104 64-65 1.1308722577805526 1104 66-67 1.1808161646489026 1104 68-69 1.099856140233591 1104 70-71 1.0885565348490154 1104 72-73 1.0833422485567148 1104 74-75 1.3434059998800727 1104 76-77 1.2012958110909473 1104 78-79 1.1359746236028059 1104 80-81 1.1658519828127822 1104 82-83 1.1844694986896478 1104 84-85 1.1514817060364457 1104 86-87 1.2783402486909914 1104 88-89 1.2972058227826047 1104 90-91 1.3769500994017179 1104 92-93 1.5350344723604827 1104 94-95 1.4615354685061597 1104 96-97 1.555173701822735 1104 98-99 1.4367310237930333 1104 100-101 1.5765336156102059 1105 1 0.13809267881791953 1105 2 0.28229863060143146 1105 3 0.20782551635844726 1105 4 0.12458323778115954 1105 5 0.15562052320063913 1105 6 0.17016928869855974 1105 7 0.24654320561639054 1105 8 0.06624051795459707 1105 9 0.29961691368654186 1105 10-11 0.31397989610072585 1105 12-13 0.26056235722520427 1105 14-15 0.4849652490809362 1105 16-17 0.3289879406485028 1105 18-19 0.3805900475495747 1105 20-21 0.3402541171791782 1105 22-23 0.423530721720482 1105 24-25 0.3728549635229186 1105 26-27 0.35734246260343383 1105 28-29 0.41589002099387073 1105 30-31 0.46274138369498274 1105 32-33 0.42283149808289267 1105 34-35 0.5447263662348476 1105 36-37 0.6503824674922924 1105 38-39 0.6283726098844298 1105 40-41 0.6645492356371037 1105 42-43 0.7087771513982304 1105 44-45 0.5075273168765619 1105 46-47 0.6332860374555835 1105 48-49 0.5939850006195044 1105 50-51 0.5852768305998168 1105 52-53 0.592242415719717 1105 54-55 0.7798726185235125 1105 56-57 0.7685751710125928 1105 58-59 0.8900837803050408 1105 60-61 0.9599010017905485 1105 62-63 0.9604445080742039 1105 64-65 0.8454381514956495 1105 66-67 0.9654364618586655 1105 68-69 1.0329228496669955 1105 70-71 0.987072872603175 1105 72-73 0.9108686603603395 1105 74-75 0.724438568471534 1105 76-77 0.7370012666187868 1105 78-79 0.8424826142055792 1105 80-81 0.8609833521217247 1105 82-83 0.9160976991549283 1105 84-85 0.8504969254594208 1105 86-87 0.8271678385846748 1105 88-89 0.952580351560897 1105 90-91 0.941273473239356 1105 92-93 0.9561532235716506 1105 94-95 1.087059671331243 1105 96-97 1.0509060965788457 1105 98-99 0.8452479056775992 1105 100-101 0.8673307475656493 1106 1 0.05209217600409488 1106 2 0.059745496717582114 1106 3 -0.12084711961079364 1106 4 0.06608600648035434 1106 5 0.08195829805607957 1106 6 0.09002205449690592 1106 7 0.11697195109589842 1106 8 0.1921599259235549 1106 9 0.21674932777306566 1106 10-11 -0.015825682554186926 1106 12-13 -0.25525532763945336 1106 14-15 -0.09696278407898973 1106 16-17 0.012044404062351077 1106 18-19 0.08242306178951964 1106 20-21 0.04860598371433866 1106 22-23 0.13469480018522972 1106 24-25 -0.024421139283170135 1106 26-27 0.08288024777299796 1106 28-29 0.2880141515449708 1106 30-31 0.26957062683125343 1106 32-33 0.14928788543002725 1106 34-35 0.31692591885946797 1106 36-37 0.13183798458474172 1106 38-39 0.18936940605788521 1106 40-41 0.25512663351110376 1106 42-43 0.3170884060476524 1106 44-45 0.07443862180736716 1106 46-47 0.18634256533274396 1106 48-49 0.13504812953968326 1106 50-51 0.23748598825773826 1106 52-53 0.31848768572429265 1106 54-55 0.14318944924930577 1106 56-57 0.2516471056210108 1106 58-59 0.4094276082653536 1106 60-61 -0.002792468720706154 1106 62-63 0.21513260234534926 1106 64-65 0.3288726922267955 1106 66-67 0.37115725836733304 1106 68-69 0.12261226543601111 1106 70-71 -0.11597817589257531 1106 72-73 0.3075710397011342 1106 74-75 0.34500913323460125 1106 76-77 0.44599454309548037 1106 78-79 0.41629149338199767 1106 80-81 0.20449544221564864 1106 82-83 0.14083613927173744 1106 84-85 0.30203179688734494 1106 86-87 0.34162695917619246 1106 88-89 0.6131504006174744 1106 90-91 0.495501621788609 1106 92-93 0.37911634411159056 1106 94-95 0.5934415390912129 1106 96-97 1.0563145683471404 1106 98-99 0.8319426783477333 1106 100-101 0.5960385160392221 1107 1 0.3392643379314393 1107 2 0.45422618353926225 1107 3 0.4628482284387445 1107 4 0.41986396751529753 1107 5 0.3495522132636424 1107 6 0.29950708549518623 1107 7 0.37874172446241516 1107 8 0.3910495838380541 1107 9 0.5642981813522283 1107 10-11 0.6098461922157199 1107 12-13 0.574990449673642 1107 14-15 0.5422165346952212 1107 16-17 0.6702902402795345 1107 18-19 0.6902138639159574 1107 20-21 0.7169943678279083 1107 22-23 0.6316133800076287 1107 24-25 0.7243013782776089 1107 26-27 0.8066495204547621 1107 28-29 0.7103417466382567 1107 30-31 0.8624108347626915 1107 32-33 0.8129160828176296 1107 34-35 0.7243728683639361 1107 36-37 0.720889923265581 1107 38-39 0.7450595410955074 1107 40-41 0.8388624781221097 1107 42-43 0.8792138128540188 1107 44-45 1.0028664716608375 1107 46-47 0.9179976229851192 1107 48-49 1.046651498853599 1107 50-51 0.6494206930746174 1107 52-53 0.7042027077396966 1107 54-55 0.7858809243549629 1107 56-57 0.8741087075177276 1107 58-59 0.7483911908120717 1107 60-61 0.9772574524950741 1107 62-63 1.0043001403016731 1107 64-65 0.9856753782532692 1107 66-67 1.0797219129684805 1107 68-69 1.1296439411100963 1107 70-71 0.9799618597444031 1107 72-73 0.9281145771862249 1107 74-75 0.9239074795022901 1107 76-77 1.0836460370504497 1107 78-79 1.123549213499217 1107 80-81 1.282355603907373 1107 82-83 1.2852137439369393 1107 84-85 1.0864722816680015 1107 86-87 1.1626947430570844 1107 88-89 1.38917864512835 1107 90-91 1.4164928303532633 1107 92-93 1.5356981398027578 1107 94-95 1.9015798753452025 1107 96-97 2.012338984092203 1107 98-99 2.039542778484684 1107 100-101 1.9282980769092362 1108 1 -0.16373289043802686 1108 2 -0.1344409271612328 1108 3 -0.11921566946212181 1108 4 -0.029165245613775426 1108 5 -0.04827703424224694 1108 6 -0.051598343892692355 1108 7 -0.0017379753134179055 1108 8 0.05471902213525226 1108 9 0.04348230499416417 1108 10-11 0.02806339637817956 1108 12-13 0.0193304506762928 1108 14-15 0.04080923391959601 1108 16-17 0.05551465641084974 1108 18-19 0.0510987803731382 1108 20-21 0.012253590482224297 1108 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-0.6374107093543415 2316 88-89 -0.6362744938878926 2316 90-91 -0.8393003508056509 2316 92-93 -0.9255166260239598 2316 94-95 -0.8076842841421161 2316 96-97 -0.6361007223068427 2316 98-99 -1.0481545678290765 2316 100-101 -1.1909124516739666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6658.0 3 1257.0 4 1165.0 5 1210.0 6 1428.0 7 1743.0 8 2017.0 9 2619.0 10 3282.0 11 4563.0 12 6110.0 13 7897.0 14 9290.0 15 11878.0 16 15702.0 17 21076.0 18 17318.0 19 11311.0 20 10804.0 21 11468.0 22 12437.0 23 13453.0 24 15180.0 25 17403.0 26 20214.0 27 23286.0 28 27703.0 29 33438.0 30 40374.0 31 50391.0 32 63446.0 33 83373.0 34 113128.0 35 159498.0 36 230699.0 37 314267.0 38 231658.0 39 23156.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72456812988455 18.955766949115844 15.247306959038065 22.07235796196153 2 14.877044922754234 20.048158023616477 41.66794461456267 23.40685243906662 3 18.839910291441512 24.999613538399135 30.54363707978484 25.616839090374512 4 12.26125451488451 16.65274300297251 38.16297921468063 32.92302326746235 5 13.161058509947496 37.33572873054255 34.668736355821615 14.834476403688335 6 30.80625581115185 36.48152323997393 17.931739623329246 14.780481325544976 7 27.719372597183 33.501887039568835 20.984596641817095 17.794143721431073 8 25.985377649328008 34.80660662367526 20.166462994974726 19.04155273202201 9 26.36312583750296 16.889187354160963 21.045982117207476 35.7017046911286 10-11 24.508632449905594 26.980329234175905 27.495854298678317 21.015184017240184 12-13 25.524129591047178 24.4929982717693 28.448499806172517 21.534372331011006 14-15 22.454934911732444 25.533387718993144 27.27387298281662 24.737804386457785 16-17 21.151942794866663 29.06358350076239 27.908755489325316 21.87571821504563 18-19 21.0498418176056 27.019597813308327 31.21512249637209 20.715437872713984 20-21 21.89004847014652 25.979664962042065 31.329202576055632 20.80108399175578 22-23 21.537242717007242 25.911486002322654 31.31013565268958 21.24113562798053 24-25 21.42138561762034 26.107981668746955 31.388239989053385 21.082392724579314 26-27 21.147659464067306 26.367814387902598 31.321825235041945 21.162700912988154 28-29 20.91522993343451 26.358624554137243 31.62199055189523 21.104154960533016 30-31 20.994090605363095 26.21214569195585 31.53192923554756 21.261834467133493 32-33 20.934599245407988 26.30203884421935 31.348932848231115 21.414429062141547 34-35 21.440533374358328 26.337768543035 31.020950859285197 21.20074722332147 36-37 21.34964402589846 26.154522285597103 31.078027694698623 21.417805993805818 38-39 21.19054118989884 26.398093463993348 31.17616867050181 21.235196675605998 40-41 21.182617600996196 26.302943341078024 31.238366370499076 21.27607268742671 42-43 21.164742849041552 26.276936458266732 30.917078935363 21.641241757328718 44-45 21.268174944029575 26.577674101384314 30.578354889566516 21.575796065019595 46-47 21.456619216070514 26.81661936434076 30.004645800924962 21.722115618663764 48-49 21.563717899651788 26.94633048195968 29.676248159395215 21.813703458993317 50-51 21.503520265311117 27.086365736908046 29.61918983237656 21.79092416540428 52-53 21.704758335297935 27.301636640426985 29.316562267202272 21.677042757072805 54-55 21.43384195900829 27.209677055693295 29.388861588811 21.96761939648742 56-57 21.51296892713554 27.528725391526 29.31390421050454 21.644401470833927 58-59 21.561097780536535 27.034374353415664 29.587760988459532 21.816766877588268 60-61 21.639407540533572 26.947827067053897 29.84614149424169 21.566623898170835 62-63 21.515768968034656 27.064307446391933 29.79199118504125 21.62793240053216 64-65 21.497013458736568 26.97062004314751 29.69732511393697 21.83504138417896 66-67 21.284479540098836 27.269790792033135 29.459546342929123 21.98618332493891 68-69 21.32981176035309 27.10069065367654 29.605240604617677 21.96425698135269 70-71 21.373341173244327 27.236598137444673 29.499445976878025 21.89061471243297 72-73 21.535703642857033 27.077114062975276 29.391539579186936 21.995642714980754 74-75 21.348661597460744 27.354348858625734 29.261570160902302 22.035419383011224 76-77 21.432802430139063 27.287242387594336 29.17383672163469 22.106118460631922 78-79 21.488652089975353 27.1950508800587 29.18896114745658 22.12733588250936 80-81 21.294745898697947 27.554184251469525 29.08464114031669 22.066428709515836 82-83 21.455442584840238 27.42683907499342 28.991873272962422 22.12584506720392 84-85 21.36958552382717 27.10261932884041 29.03612095385874 22.491674193473678 86-87 21.38093283940416 27.05176220873664 29.25708988763698 22.310215064222216 88-89 21.46297132333369 26.910135987662976 29.202669728277886 22.424222960725448 90-91 21.276783009332302 27.089994246638543 29.3698756711869 22.263347072842247 92-93 21.330575171790663 26.858725125363257 29.451897224206235 22.35880247863984 94-95 21.06130588959363 26.93978235688123 29.37857852899587 22.620333224529272 96-97 21.237642703555508 26.898969789106463 29.27783331156244 22.585554195775583 98-99 21.16415400298376 26.9528073801563 29.06283959870261 22.82019901815733 100-101 21.36832807072306 26.415277840813832 29.662103061946897 22.554291026516207 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2159.0 1 1277.0 2 437.0 3 482.5 4 408.0 5 367.0 6 648.0 7 1484.0 8 2542.5 9 3075.5 10 2752.0 11 2344.5 12 2457.5 13 2725.0 14 2922.0 15 3079.0 16 3297.5 17 3456.5 18 3610.0 19 3721.5 20 4000.5 21 4526.5 22 5299.0 23 6195.5 24 7285.5 25 8932.0 26 11099.0 27 13570.5 28 15574.5 29 17870.5 30 20843.5 31 23934.0 32 27196.0 33 31049.5 34 35785.0 35 39458.0 36 43632.0 37 47964.0 38 50014.0 39 50615.5 40 51030.0 41 52982.5 42 55444.0 43 57666.0 44 59129.0 45 60243.0 46 60018.5 47 60172.0 48 64743.5 49 64680.0 50 59874.0 51 56473.0 52 53000.0 53 50646.0 54 48072.0 55 43998.0 56 38973.5 57 33629.0 58 29213.5 59 25529.5 60 21536.5 61 17398.0 62 13497.5 63 10025.5 64 7210.5 65 5305.5 66 3870.5 67 2901.5 68 2118.0 69 1406.0 70 1023.5 71 775.5 72 607.0 73 473.5 74 370.5 75 280.5 76 173.5 77 118.5 78 75.5 79 55.0 80 39.0 81 28.5 82 23.5 83 17.0 84 8.5 85 6.0 86 7.0 87 5.5 88 4.5 89 4.0 90 3.5 91 3.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32868756184856696 2 0.2915707146842756 3 0.28780970525400684 4 0.29311211199176274 5 0.30303871065198024 6 0.20038164997333383 7 0.19927184391194305 8 0.20266291798841488 9 0.20062827354253177 10-11 0.20722545401857692 12-13 0.19714471562761074 14-15 0.19843948936589997 16-17 0.2040193476190036 18-19 0.1975454789275574 20-21 0.18949938498247432 22-23 0.18897530989792866 24-25 0.19356867387424048 26-27 0.18774219205193893 28-29 0.20183056344237177 30-31 0.20842774391841692 32-33 0.2105240442565995 34-35 0.20836608802611745 36-37 0.18293303245257891 38-39 0.1752877018074425 40-41 0.18160743076813995 42-43 0.19227390013595125 44-45 0.19686726411226305 46-47 0.19970343515803946 48-49 0.19800789811980357 50-51 0.20204635906541998 52-53 0.22698616750056264 54-55 0.1890061378440784 56-57 0.1740854119076025 58-59 0.17386961628455427 60-61 0.16785816678535426 62-63 0.17504107823824452 64-65 0.17312974557696043 66-67 0.1710642731849276 68-69 0.18074424827594712 70-71 0.17504107823824452 72-73 0.17124924086182608 74-75 0.1755959812689399 76-77 0.17898705534541173 78-79 0.18191571022963737 80-81 0.17547266948434095 82-83 0.17282146611546298 84-85 0.16600849001636964 86-87 0.16304900718599424 88-89 0.16733409170080862 90-91 0.17581177689198813 92-93 0.1671182960777604 94-95 0.17180414389252147 96-97 0.17081764961572965 98-99 0.1730372617385112 100-101 0.15176597889518806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1621905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39749420802333 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69085125956283 39.88304297524838 2 13.80421371155709 14.742208434183718 3 4.82144813122569 7.72359745984226 4 2.3135098263950757 4.941425102205444 5 1.1794623450533002 3.1490166869282605 6 0.7416712428949123 2.3762031538043127 7 0.48856873897778597 1.8261842486851314 8 0.33629298229205584 1.4365762059311158 9 0.2217611515893142 1.0657340826799488 >10 1.2508272263893923 12.054329022980346 >50 0.09925477858010513 3.6809842160579977 >100 0.04936420090271301 4.639804230884634 >500 0.0019652032439975912 0.6884479807887115 >1k 4.624007632935509E-4 0.3972788670632249 >5k 3.4680057247016315E-4 1.3951673327164402 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9897 0.6102083660880262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7617 0.469632931645195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5088 0.31370518001979153 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2206 0.13601289841266906 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1921 0.11844096910731516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.2483838449231E-5 0.0 22-23 0.0 0.0 0.0 2.1579562304820566E-4 0.0 24-25 0.0 0.0 0.0 4.0076329994666765E-4 0.0 26-27 0.0 0.0 0.0 7.706986537435916E-4 0.0 28-29 0.0 0.0 0.0 0.0017263649843856452 0.0 30-31 0.0 0.0 0.0 0.0033602461303220595 0.0 32-33 0.0 0.0 0.0 0.004870815491659499 0.0 34-35 0.0 0.0 0.0 0.007152083506740531 0.0 36-37 0.0 0.0 0.0 0.011036404721608231 0.0 38-39 0.0 0.0 0.0 0.015938048159417476 0.0 40-41 0.0 0.0 0.0 0.02198032560476723 0.0 42-43 0.0 0.0 0.0 0.0319069242649847 0.0 44-45 0.0 0.0 0.0 0.04013798588696625 0.0 46-47 0.0 0.0 0.0 0.055675270746437064 0.0 48-49 0.0 0.0 0.0 0.07429535022088224 0.0 50-51 0.0 0.0 0.0 0.08490016369639405 0.0 52-53 0.0 0.0 0.0 0.1024412650555982 6.165589229948733E-5 54-55 0.0 0.0 0.0 0.12818260009063415 6.165589229948733E-5 56-57 0.0 0.0 0.0 0.17143420853872454 6.165589229948733E-5 58-59 0.0 0.0 0.0 0.22624629679296876 6.165589229948733E-5 60-61 0.0 0.0 0.0 0.29126243522277817 6.165589229948733E-5 62-63 0.0 0.0 0.0 0.36053283022125215 6.165589229948733E-5 64-65 0.0 0.0 0.0 0.43791097505710874 6.165589229948733E-5 66-67 0.0 0.0 0.0 0.5336625757982125 6.165589229948733E-5 68-69 0.0 0.0 0.0 0.650161384298094 6.165589229948733E-5 70-71 0.0 0.0 0.0 0.7366029453019751 6.165589229948733E-5 72-73 0.0 0.0 0.0 0.8400923605266646 6.165589229948733E-5 74-75 0.0 0.0 0.0 0.9567761367034444 6.165589229948733E-5 76-77 0.0 0.0 0.0 1.0932206263622097 6.165589229948733E-5 78-79 0.0 0.0 0.0 1.2445550140113015 6.165589229948733E-5 80-81 0.0 0.0 0.0 1.4546166390756547 6.165589229948733E-5 82-83 0.0 0.0 0.0 1.6807396240840247 6.165589229948733E-5 84-85 0.0 0.0 0.0 1.97474574651413 6.165589229948733E-5 86-87 0.0 0.0 0.0 2.334723673704687 6.165589229948733E-5 88-89 0.0 0.0 0.0 2.745906819449968 6.165589229948733E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 43.586716 1 GTATCAA 9145 0.0 38.01122 1 TCAACGC 11360 0.0 30.177366 4 ATCAACG 11395 0.0 30.081884 3 CAACGCA 11585 0.0 29.59127 5 TATCAAC 11570 0.0 29.585796 2 AACGCAG 11865 0.0 28.943563 6 GTACATG 7530 0.0 26.334143 1 ACGCAGA 13215 0.0 25.844612 7 TACATGG 7635 0.0 25.405937 2 CGCAGAG 13325 0.0 25.34529 8 GAGTACT 7580 0.0 24.15814 12-13 ACATGGG 7765 0.0 24.000963 3 GCAGAGT 13840 0.0 23.956736 9 GTACTTT 8240 0.0 22.164812 14-15 AGTACTT 7770 0.0 21.580524 12-13 ACCGTGC 270 1.0022632E-9 21.111172 8 GGACCGC 225 7.158269E-8 21.110521 6 AGAGTAC 12170 0.0 20.862455 10-11 CATGGGA 5030 0.0 20.701439 4 >>END_MODULE