FastQCFastQC Report
Thu 26 May 2016
SRR937819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621905
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92430.5698854125241614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.4936170737496956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.31913089854214644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.12392834352196955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72050.036.2114751
GTATCAA107500.031.033911
TCAACGC131000.025.3887234
ATCAACG131100.025.3693583
TATCAAC132550.025.0918352
CAACGCA133050.024.9261195
AACGCAG136500.024.3574736
GAGTACT81800.023.43134512-13
GTACTTT87050.022.07072414-15
CGTATAG1100.002869643321.6015641
GTACATG77700.021.468121
AGTACTT85400.021.21991512-13
CGCAGAG155150.021.0947538
ACGCAGA155850.021.0304857
TACATGG79800.020.7795752
GTATAGG5300.019.7267131
GCAGAGT163600.019.714859
ACATGGG79600.019.6976783
ACTTTTT100400.019.20698416-17
GTGTAGC6200.019.162681
Produced by FastQC (version 0.11.5)